鸡腿蘑液体菌种培养及应用研究

研究结果表明,培养鸡腿蘑液体菌种的适宜培养基配方为蔗糖2%,玉米粉3%,麦麸4%,酵母粉0.5%,KH2PO4 0.25%,MgSO4*7H2O 0.1%,pH6.2;最适培养条件是250mL三角瓶装液量60mL,接种量5%,摇床转速120r/min,培养6d.利用液体菌种扩繁固体生产种,可以明显缩短菌种生产周期.     

Study on the Relationship of Taiwan＇s aboriginal oral traditions and written literature

台湾原住民文学包括口传文学与书面文学两部分。口头传统与当代台湾原住民书面文学的关系不单单是源与流的关系,应该说当代台湾原住民书面文学是台湾原住民口头传统历史的叙述的文字发展＂,更是口头传统的历史延伸,两者紧紧地结合在一起,形成了当代台湾原住民文学独一无二的审美气质。     

NaCl筛选法获得无标记耐盐转基因水稻的高效转化技术研究

NaCl筛选法是培养无标记耐盐转基因水稻的有效方法。以中华11、秀水11、春江06这3个粳稻品种成熟胚愈伤组织为材料,以SKC1为目标基因,研究NaCl筛选条件下,热激与液体共培养对农杆菌介导耐盐基因转化水稻效率的影响。结果显示,热激处理和液体共培养都能提高耐盐基因对水稻的转化率,其中,热激处理最高转化提高率达到46.2%,液体共培养后最高转化提高率达到60.7%。     

食用菌发酵的应用研究进展

综述了近年来有关食用菌发酵的应用研究概况,展望了我国食用菌发酵的应用前景。     

利用RP-HPLC分析柑橘果肉水溶性蛋白质的差异

本文探索了利用超声波细胞破碎仪提取柑橘果肉中水溶性蛋白质的方法,并对流动相及波长等色谱条件进行优化,建立了反相高效液相色谱分离柑橘果肉水溶性蛋白质的技术。优化后的色谱条件为:流动相A为0.05%三氟乙酸甲醇溶液,B为0.05%三氟乙酸水溶液,A液100%～72%,梯度洗脱45 min,再用A液平衡10min,流速为1.0 mL/min,进样量为20μL,检测波长为270 nm。从7个柑橘品种的果肉中共分离出20个不同的蛋白质峰,图谱中各品种的特有蛋白质反映了不同品种不同表型性状的重要信息。     

甘南州尖顶羊肚菌菌丝体液体发酵培养研究

对甘南藏族自治州尖顶羊肚菌进行液体发酵培养,以干菌丝体得率为指标,设计正交试验优化其液体发酵培养基和培养条件。实验结果表明最佳培养基组合为白砂糖30g·L^-1、豆粕粉15g·L^-1、KH2PO4 0．75g·L^-1、MgSO4·7H2O0．5g·L^-1、维生素Bt0．03g·L^-1。最优液体发酵条件为温度25℃,培养液初始pH7,接种量10mL,转速120r·min^-1,培养时间8d。     

苏云金杆菌CF1263菌株发酵条件优化的研究

对高毒BT菌株——CF1263进行液体振荡培养和正交试验结果表明:依据碳源、氮源和无机盐对芽孢产量的影响,对液体培养基进行成分优化组合,在碳源和氮源中分别筛选出最佳因子,淀粉、蛋白胨、牛肉膏、麦麸、酵母粉,从无机盐中筛选出KH2PO4和MgSO4.7H2O。运用以上7个因素进行L18(37)因素3水平的正交试验,从而得出CF1263最优配方为1.5%淀粉、2%蛋白胨、0.2%牛肉膏、0.3%麦麸、2.0%酵母粉、0.1%KH2PO4和0.2%Mg-SO4.7H2O。     

Preparation and evaluation of self-microemulsifying drug delivery system of baicalein

The main object of this work is to prepare self-microemulsifying drug delivery system (SMEDDS) for oral bioavailability enhancement of a poorly water-soluble drug, baicalein. SMEDDS is the mixture of surfactants, cosurfactants, and oils, which are emulsified in aqueous media under conditions of gentle agitation or gastrointestinal motility. Solubility of baicalein was determined in various vehicles. Pseudo-ternary phase diagrams were constructed to identify the efficient self-emulsification region and droplet size distributions of the resultant microemulsions were determined using a particle size analyzer. Optimized SMEDDS formulations for baicalein were Cremophor RH40 (53.57%) as surfactant, Transcutol P (21.43%) as cosurfactant, and Caprylic capric triglyceride (ODO, 25%) as oil. The drug release rate of SMEDDS was significantly higher than that of the baicalein suspension. Comparison of the pharmacokinetics between baicalein-loaded SMEDDS and baicalein suspension was also performed in rats. The plasma concentrations of baicalein and baicalin, its mainly conjugated metabolite, were determined by HPLC method. The in vivo results showed that the absorption of baicalein from SMEDDS resulted in about 200.7% increase in relative bioavailability compared with that of the baicalein suspension. Our studies illustrated the potential use of SMEDDS for the delivery of hydrophobic compounds, such as baicalein by the oral route.     

高效液相色谱法测定鸡蛋中氟喹诺酮类药物残留量前处理方法的优化

建立了鸡蛋中环丙沙星、达氟沙星、恩诺沙星和沙拉沙星4种氟喹诺酮类药物残留测定的超高效液相色谱方法。样品经磷酸盐缓冲液提取后,用正己烷脱脂,C18固相萃取柱净化,反相高效液相色谱分离,荧光检测器测定。本方法的检测限为环丙沙星、恩诺沙星和沙拉沙星5 μg/kg,达氟沙星1 μg/kg。环丙沙星、恩诺沙星和沙拉沙星在2~200 ng/mL、达氟沙星在0.4～40 ng/mL范围内呈线性相关,相关系数r大于0.9999。在空白鸡蛋中添加环丙沙星、恩诺沙星和沙拉沙星5~50 μg/kg、达氟沙星1～10 μg/kg,4种氟喹诺酮类药物的平均回收率为82.2～101.3％,批内变异系数0.8～5.9％之间(n＝6),批间变异系数在1.0～6.6％之间(n＝4)。结果表明,该法灵敏、准确、特异性强,适用于鸡蛋中氟喹诺酮类药物残留的测定。