| NaCl筛选法获得无标记耐盐转基因水稻的高效转化技术研究 |
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| 引用本文： | 赵潇,罗园园,赵艳.NaCl筛选法获得无标记耐盐转基因水稻的高效转化技术研究[J].华北农学报,2012(1):63-67. |
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| 作者姓名： | 赵潇 罗园园 赵艳 |
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| 作者单位： | 浙江工商大学食品与生物工程学院 |
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| 基金项目： | 国家自然科学基因资助项目(30871511;30771317);农业部转基因生物新品种培育重大专项(2008ZX0810-003;2009ZX08001-022B);国家“863”计划资助项目(2006AA10Z1A9);中国博士后基因资助项目(20090450477) |
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| 摘 要： | NaCl筛选法是培养无标记耐盐转基因水稻的有效方法。以中华11、秀水11、春江06这3个粳稻品种成熟胚愈伤组织为材料,以SKC1为目标基因,研究NaCl筛选条件下,热激与液体共培养对农杆菌介导耐盐基因转化水稻效率的影响。结果显示,热激处理和液体共培养都能提高耐盐基因对水稻的转化率,其中,热激处理最高转化提高率达到46.2%,液体共培养后最高转化提高率达到60.7%。
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| 关 键 词： | 水稻 NaCl筛选 热激 液体共培养 转化效率 |
Study on NaCl Screening to Obtain Marker-free and High Effective Salt-tolerant Transgenic Rice |
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| ZHAO Xiao,LUO Yuan-yuan,ZHAO Yan.Study on NaCl Screening to Obtain Marker-free and High Effective Salt-tolerant Transgenic Rice[J].Acta Agriculturae Boreali-Sinica,2012(1):63-67. |
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| Authors: | ZHAO Xiao LUO Yuan-yuan ZHAO Yan |
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| Institution: | (College of Food Science,Biotechnology Engineering,Zhejiang Gongshang University,Hangzhou 310012,China) |
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| Abstract: | It is an effective method to use NaCl selecting to generate marker-free salt-tolerant transgenic rice.We used the three japonica rice cultivar(ZhongHua11,Xiushui11,Chunjiang06)mature embryos callus as materials,SKC1 as the target gene to study that on the conditions of screening NaCl,heat shock treatment and liquid co-culture of Agrobacterium-mediated gene transfer salt-tolerant rice efficiency.The results showed that heat shock and liquid co-culture of rice could enhance the efficiency of salt tolerance gene transfer.Among them,the heat shock treatment increased the maximum efficiency of gene transfer was 46.2%,while the liquid co-culture increased the highest efficiency of gene transfer was 60.7%. |
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| Keywords: | Rice NaCl screening Heat shock Liquid co-culture The efficiency of gene transfer |
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