多营养层次生态养殖模式简析

系统介绍了多营养层次生态养殖模式在淡水养殖及海水养殖中的应用与发展,对比了国内外有关该养殖模式发展方向的异同,简述了该养殖模式通过提高物质和空间利用率、改善养殖环境的原理以及在我国推广应用情况,并汇总了我国沿海省份近年来开展该养殖模式选用的品种搭配及产量产值。指出了多营养层次生态养殖模式还存在的问题,对今后发展提出了建议。     

寨卡病毒SYBR GreenⅠreal-time PCR方法的建立及应用

为建立一种针对寨卡病毒的快速诊断方法,本研究根据寨卡病毒的3’端保守基因序列,设计合成1对引物,建立了检测寨卡病毒的荧光定量PCR方法。结果显示:所建立的检测方法的Ct值与标准品在1.41×10^1~1.41×10^10^ copies/μL具有良好的线性关系,相关性为1,斜率为-3.502;灵敏性结果显示,该方法的检测限度为1.41×10^1 copies/μL,是普通PCR的10000倍;特异性结果显示,对CHIKV、DENV和JEV无特异性扩增,特异性强;重复性试验结果显示,组内和组间变异系数均小于1%,重复性好。本研究建立的SYBR Green I real-time PCR检测方法,可用于寨卡病毒感染的快速诊断。     

弘扬茶文化 促进社会主义和谐社会建设

中国是茶的故乡,是世界上茶历史最悠久、茶资源最丰富、茶文化最深厚的国家.茶在中国始终扮演着社会交往的最佳礼物和媒介,是人与人之间友谊的桥梁.茶文化的本质是"和谐文化",它引导人们与自然和谐相处,启示人们在人与人的关系上,要彼此尊重,加强交流,启发人们感悟身心的统一.大力弘扬茶文化精神,是建设和谐文化的一笔重要资源,是构建和谐社会的重要载体.     

青饲牧草地化学除草试验报告

对６种青饲牧草用７种除草剂进行除草试验。证明播后苗前墨西哥类玉米用４０％阿特拉津胶悬剂０．９０ｋｇ（ａｉ）／ｈｍ＾２；Ｍ－８１Ｅ饲用高粱与串叶松香草适宜用６０％丁草胺乳油０．９０ｋｇ（ａｉ）／ｈｍ＾２；苏丹草适宜用６０％丁草胺乳油０．９０ｋｇ９ａｉ）／ｈｍ＾２与２５％绿麦隆可湿性粉剂０．９４ｋｇ（ａｉ）／ｈｍ＾２，紫苏用１２％恶草灵乳油０．２７ｋｇ（ａｉ）／ｈｍ＾２与８４％ｃｈｉｎｃｈ乳油１．８９     

汝城白毛茶种群个体间亲缘关系的RAPD分析

采用RAPD技术,对汝城白毛茶种群个体间的亲缘关系进行了分析。研究结果:汝城白毛茶种群个体单株扩增的谱带数最多时为13条,最少时为10条;DNA多态性最高为92.31%,最低为70.00%,平均为85.40%。结果表明汝城白毛茶种群呈现丰富的遗传多样性。汝城白毛茶种群单株间的遗传距离最小为0.0882,最大为0.5791。聚类分析结果表明汝城白毛茶种群中有些单株聚类很近,遗传基础比较一致,有些聚类较远,显示其丰富的遗传多样性。研究发现种群单株间的遗传变异呈现连续性,当结合距离I为0.14时,汝城白毛茶种群可分为6个类型,这与其表观形态类型相符。本文运用分子生物学方法证明汝城白毛茶的核心种质基因群在汝城县白毛茶示范场得到了有效保护。     

红豆草茬地放牧结合补饲青干草对草地羔羊育肥效果的研究

1986～1987年期间在甘肃农大牧草站进行了以红豆草茬地放牧结合补饲青干草对草地羔羊育肥效果的试验。比较了在放牧基础上3种补饲处理(即单燕麦草、单红豆草和燕麦+红豆草混合干草)对两种类型羔羊(即甘肃高山细毛羊和边甘杂种一代羔羊)增重和肉用性能的影响。试验结果表明,7月龄边甘杂种羔羊或9月龄甘高羔羊的期末活重均已超过35公斤,胴体重超过17公斤,屠宰率为47％～49％。     

Effects of three methods of restraint an in rhesus and african green monkeys intravenous glucose tolerance testing

The intravenous administration of 0.75 gm glucose per kg and the measurement of serum glucose pretest and at 10, 20, 30, 60, 90 and 120 minutes constitute a satisfactory protocol for intravenous glucose tolerance testing of Rhesus (Macaca mulatto) and African Green (Cercopithecus aethiops) monkeys. No significant differences were noted between animals restrained with ketamine hydrochloride and those restrained with sodium pentobarbital, but the African Green males and females and the male Rhesus monkeys yielded significantly different results while being manually restrained.     

Impact of environmental factors on infestation of tea leaves byHelopeltis theivora, and associated changes in flavonoid flavor components and enzyme activities

Attack of tea plant (Camellia sinensis (L.) O. Kuntze) leaves by the tea mosquito bugHelopeltis theivora (Hemiptera: Miridae) was positively correlated to temperature and rainfall, and partially to humidity, as determined in 12 varieties during the period 2000–2002. The insect attack was severe during the months of May–September, which had high temperature and rainfall, and led to severe loss of biomass due to curling and drying up of the leaves. The biochemical response of these 12 varieties of tea to attack by the insect was determined with special reference to oxidative enzymes and flavonoid flavor components. Insect attack led to an increase in the activities of the oxidative enzymes peroxidase, ascorbate peroxidase and polyphenol oxidase. Activities of phenyl alanine ammonia lyase generally decreased as a result of insect attack, which was significant in the United Planters Association of South India (UPASI) varieties. A significant decrease in polyphenols was also obtained in UPASI varieties. HPLC analysis of catechins revealed a decrease in some of the catechins in the infected leaves. Analysis of theaflavins from infusion of healthy andHelopeltis-infested tea leaves revealed no changes. http://www.phytoparasitica.org posting Dec. 19, 2004.     

根癌农杆菌介导绿色荧光蛋白基因转化印度酸桔的研究

 通过根癌农杆菌介导将绿色荧光蛋白基因转入印度酸桔的胚性愈伤组织中, 经潮霉素筛选,获得抗性愈伤组织, 并再生植株。对这些植株进行GUS 染色、PCR 分析、绿色荧光检测和Sourthern 杂交验证, 结果表明绿色荧光蛋白已经在转基因植株中表达。