黑龙江省半干旱区防护林内主要气象因子观测初报

对齐齐哈尔(沙壤土区)、杜蒙(风沙土区)、青冈(黑土区)等3个代表黑龙江省半干旱区典型地点防护林区内7、8、9月主要气象因子(气温、气湿、风速、降雨量等)及土壤入渗率情况进行了定期观测,结果表明:3个月份平均气温杜蒙>齐齐哈尔>青冈,气湿为青冈>齐齐哈尔>杜蒙,风速为杜蒙>齐齐哈尔>青冈,土壤入渗率为杜蒙>齐齐哈尔>青冈,揭示了黑龙江省半干旱区防护林内不同土壤类型之间主要气象因子的变化特征及规律,为半干旱区防护林功能评价提供了重要依据。     

木材表面非极性化原理的研究 Ⅱ.木材与苯乙烯接枝共聚过程中化学官能团和表面极性的变化

通过对杉木、杨木木材及其主要成分在与苯乙烯接枝共聚反应过程中化学官能团及临界表面张力变化的研究,探讨了木材各化学组成在接枝共聚过程中的反应性能和对木材表面极性的影响。研究表明:木材可与苯乙烯发生接枝共聚反应,反应只在木素和苯乙烯之间进行。木材与苯乙烯接枝共聚可降低木材临界表面张力,即降低木材表面自由能,从而有效地改善木材的极性。     

Enhanced efficiency of electrostatically charged insecticide aerosols

The bioefficacy of a standard domestic aerosol insecticide has been compared with that of a similar spray in which the droplets were electrostatically charged. The aerosol was charged without the need for an external power supply, by enhancing natural charge separation processes that occur during atomisation. The charge-to-mass ratio achieved was 1.1 x 10(-4) C kg-1, compared with 2.2 x 10(-5) C kg-1 for the standard aerosol. Efficiency was assessed for insects in free flight. A direct space-spray application method was used to study the bioefficacy of these aerosols on houseflies, Musca domestica, and an indirect space-spray method on houseflies and mosquitoes, Culex quinquefasciatus. Two levels of concentration of active ingredients were compared. At low concentrations of 1.57 g kg-1 of bioallethrin and 0.29 g kg-1 of bioresmethrin, the charged aerosol achieved a significant reduction in KDT50 (time at which 50% of the flies were knocked down), compared with the standard aerosol. In the direct spray application to houseflies, the KDT50 was reduced by 50%, while in the indirect spray the KDT50 was reduced by 40%. In the indirect spray of mosquitoes, the KDT50 was reduced by 22%. With higher active ingredient concentrations of 2.09 g kg-1 for bioallethrin and 0.39 g kg-1 for bioresmethrin, the charged aerosol also demonstrated faster knockdown than the standard, but the improvement was less marked. In the direct spray application to houseflies, the KDT50 was reduced by 21% compared with the standard aerosol, while in the indirect spray the KDT50 was reduced by 16%. In the indirect spray of mosquitoes, the KDT50 was reduced by 24%. The percentage mortality after 24 h was also increased with the charged aerosol. Charge carried by the droplets results in increased interception of the insecticide, as the droplets are attracted to the insects in flight and space charge effects within the aerosol could cause improved dispersion in the test chamber.     

Identification of Trichoderma SKT-1, a biological control agent against seedborne pathogens of rice

Trichoderma SKT-1 was previously reported as a powerful biological control agent against seedborne pathogens of rice, but the taxonomic disposition of the fungal isolate was not clear. Trichoderma SKT-1 produced irregular pyramidal warts on conidia and had an optimum growth temperature of 30°C. Morphological characteristics and colony growth were identical to those of known species of Trichoderma, including the newly recognized species T. asperellum. The 5.8S rDNA with the internal transcribed spacer (ITS) region (ca. 514 bp) of the fungus was compared with those of known species to determine the phylogenetic placement of the fungus. The length and sequence of the regions from Trichoderma SKT-1 were completely identical to those of an isolate of T. asperellum NRRL 5242 (AJ230669). On the basis of these results, we concluded that Trichoderma SKT-1 was T. asperellum.     

伊犁河流域地表水资源优势及开发利用潜力研究

伊犁河流域是新疆地表水系最发达、径流最发育、水资源最丰富的内陆河流域,具有流域降水丰沛,径流补给充分,径流量大;水系发达,河川密布;水量变化较小、泥沙含量较少、水质较好的资源优势。但受经济发展水平及产业结构层次的限制,伊犁河流域水资源总体上仍处于利用效率低下、生产经营方式落后、综合利用潜力巨大的初级开发阶段。依据部颁SL201-9《江河流域规划编制规范》和流域整体规划目标,经过水资源多目标开发平衡分析与评价,给出了伊犁河流域水资源综合开发和合理配置远景规划方案。     

塔克拉玛干沙漠及周围地区地表反射率

本文利用1988年塔克拉玛干沙漠综合科学考察期间在其腹地满西—井获得的地表反射率资料及其周围日射观测站历年反射率平均值资料,分析了沙漠地表反射率的日变化及地表性状、降水等因子对反射率的影响,并讨论了沙漠及周围地区地表反射率的时空分布状况。     

犬声带切除术的研究

犬声带切除术是目前小动物临床外科的常见手术，主要目的是消除或降低犬的吠叫声音。切除声带的手术途径有两种，即经口腔切除和经腹侧喉室切除。选取20只2岁左右的杂种犬，随机分成2组。第l组采用腹侧喉室声带切除术，术后半年的消声率为80oA；第2组采用经口腔声带切除术，术后半年的消声率为40％。但采用经口腔声带切除术，手术比较简便和快捷，损伤小，恢复较快。因此，经口腔声带切除术是一种较好的犬声带切除方法。     

柔嫩艾美耳球虫表面抗原SAG2基因的克隆与表达

根据生物信息学预测的基因序列设计引物,应用RT-PCR方法从柔嫩艾美耳球虫第二代裂殖子总RNA扩增获得了鸡柔嫩艾美耳球虫表面抗原2(surface antigen 2,SAG2)基因序列,将其与pGEM-T easy载体连接后转化E.coliDH5α,筛选阳性克隆,以带有限制酶切位点的特异性引物用PCR方法扩增不含SAG2 N端信号肽序列的ORF序列后克隆至表达载体pET-32 a(+),构建了重组表达质粒pET-32 a(+)-SAG2,并将其转化至E.coliBL21(DE3)。经IPTG诱导,获得了SAG2重组抗原在大肠杆菌的高效表达,重组蛋白的表达量约占菌体总蛋白的35%,融合蛋白的分子量约为47 ku。菌体经超声处理后进行SDS-PAGE分析表明,表达蛋白以包涵体的形式存在。     

Implantation study on the potential of murine epidermal stem cell differentiation

AIM: To investigate the potential of murine epidermal stem cell (ESC) differentiation after seeded in a biodegradable carrier and implanted subcutaneously into syngeneic recipient mice. METHODS: ES cells were induced in vitro to differentiate into ESCs. After stained with a fluorescent dye Hoechst 33342, these ESCs were seeded into a polyglycolic acid (PGA) net containing collagen gel, functioning as a cell carrier, and implanted subcutaneously into 129/J mice, which were syngeneic to these stem cells. RESULTS: The ESCs kept alive in the implant when observed under a fluorescent microscopy 3 weeks or longer after implantation, and could differentiate into hair follicle-like structure, glandular structure, and gave rise to additional structures displaying features resembling native dermis. No apparent rejection or severe side effects were observed at least 10 weeks post-implantation. CONCLUSION: It is feasible to use these ESCs as seed cells in the study to fabricate dermal equivalent having the potential to develop dermal appendages.     

牛瑟氏泰勒虫P33表面蛋白基因的克隆与序列分析

为分析吉林省流行的牛瑟氏泰勒虫基因序列，根据GenBank上发表的牛瑟氏泰勒虫P33表面蛋白基因序列设计合成一对引物，用PCR方法扩增出牛瑟氏泰勒虫的基因片段，并成功地将该基因纯化后克隆到pGEM-TEasy载体上，将经EcoRⅠ酶切鉴定和PCR鉴定为阳性的重组质粒进行测序。结果表明克隆的基因片段长度为868bp，编码283个氨基酸，有2个潜在的糖基化位点。核苷酸同源性分析表明，该基因片段与韩国株（AF521557）、日本株（AB016280）、俄罗斯株（AB016279）的核苷酸序列同源性分别为99．4％、88.0％、88．1％。