Expression profiling of transgenes (Cry1Ac and Cry2A) in cotton genotypes under different genetic backgrounds

Transgenic cotton carrying the Cry1Ac gene has revolutionized insect pest control since its adoption, although the development of resistance in insect pests has reduced its efficacy.  After 10 years of cultivating Bacillus thuringiensis (Bt) cotton with a single Cry1Ac gene, growers are on the verge of adopting Bt cotton that carries the double gene (Cry1Ac+Cry2A) due to its better effectiveness against insect pests.  Thus, the current study was designed to evaluate the role of each gene in the effectiveness of Bt cotton carrying the double gene.  The expression levels of the Cry1Ac and Cry2A genes were evaluated in the leaves of 10 genotypes (2 parents and 8 F₁ hybrids) at 30 days after sowing (DAS), while samples of leaves, bolls and flowers were taken from the upper and lower canopies at 70 and 110 DAS.  The F₁ hybrids were developed through reciprocal crosses between two Bt (CKC-1, CKC-2) and two non-Bt (MNH-786, FH-942) parents.  The differential expression of transgenes was evaluated through Enzyme Linked Immuno-Sorbent Assay (ELISA).  The results showed that the MNH786×CKC-1 hybrid had the highest concentrations of Cry1Ac gene at 30 DAS (3.08 µg g^–1) and 110 DAS (1.01 µg g^–1) in leaves.  In contrast, the CKC-2×MNH-786 hybrid showed the lowest concentrations of Cry1Ac gene at 30 DAS (2.30 µg g^–1) and 110 DAS (0.86 µg g^–1).  The F₁ hybrid FH-942×CKC-2 showed the highest concentrations of Cry2A gene at 30 DAS (8.39 µg g^–1) and 110 DAS (7.74 µg g^–1) in leaves, while the CKC-1×MNH-786 hybrid expressed the lowest concentrations of Cry2A gene at 30 DAS (7.10 µg g^–1) and 110 DAS (8.31 µg g^–1).  A comparison between the two stages of plant growth showed that leaves had the highest concentrations at 30 DAS, whereas the lowest concentrations were observed at 110 DAS for both genes in leaves.  When the expression pattern was compared between various plant parts in genotype CKC-2, it was found that leaves had higher concentrations of Cry1Ac (3.12 µg g^–1) and Cry2A (8.31 µg g^–1) at 70 DAS, followed by bolls (Cry1Ac (1.66 µg g^–1) and Cry2A (8.15 µg g^–1)) and flowers (Cry1Ac (1.07 µg g^–1) and Cry2A (7.99 µg g^–1)).  The genotype CKC-2 had higher concentrations of Cry1Ac (3.12 µg g^–1) and Cry2A (8.31 µg g^–1) in the upper canopy but less accumulation (2.66 µg g^–1 of Cry1Ac, 8.09 µg g^–1 of Cry2A) in the lower canopy at 70 DAS.  Similarly, at 110 DAS, the expression levels of Cry1Ac and Cry2A in upper and lower canopy leaves were 1.52 and 7.92 µg g^–1, and 0.99 and 7.54 µg g^–1, respectively.  Hence, the current study demonstrates that different genotypes showed variable expression for both of the Cry1Ac and Cry2A genes during plant growth due to different genetic backgrounds.  The Cry2A gene had three-fold higher expression than Cry1Ac with significant differences in expression in different plant parts.  The findings of this study will be helpful for breeding insect-resistant double-gene genotypes with better gene expression levels of Cry1Ac and Cry2A for sustainable cotton production worldwide.     

叶片布置方式对双吸离心泵径向力的影响

为研究叶片布置方式对双吸离心泵径向力的影响,在其他外部因素不变的情况下,对3种叶片布置方案的离心泵进行非定常模拟计算,结果表明:较对称布置方案,采用交错30°布置的叶片能够有效提高离心泵的扬程,采用交错15°布置的叶片对离心泵扬程具有消极影响.同时,采用交错布置方案会导致离心泵的效率下降;在设计工况下,与对称布置方案相比,叶片采用交错布置能够有效降低离心泵内的压力脉动水平,且交错角度越大,压力脉动水平降低的越明显.叶片采用交错布置能够有效降低作用在叶轮上的稳态径向力的大小,其中采用交错30°布置方案在1.1Q_d工况下稳态径向力相对降低42.284%.最后,叶片采用交错30°布置对改善叶轮所受瞬态径向力的脉动特性与幅值大小的效果最为明显.     

高地隙四轮独立驱动喷雾机路径跟踪模型预测控制

针对传统燃油驱动、前轮转向的高地隙喷雾机传动效率低、碳排放高、环境污染、智能化水平低、灵活性差等问题,本研究提出了一种适用于无人驾驶的高地隙四轮独立驱动（Four Wheel Independent Drive,4WID）喷雾机。其采用混合动力、前后双转向桥的4WID,转向半径小,前后轮的运行轨迹高度一致,能够减少田间植保作业时的压苗现象。考虑水田极端作业环境下驱动轮的滑移、陷坑等问题,基于喷雾机线性时变的运动学模型（LTV）,构建了考虑驱动轮滑移的分层路径跟踪控制。上层模型预测控制（Model Predictive Control,MPC）器根据预期路径、车辆当前位置,获得喷雾机的转向角和运动速度,实现路径跟踪。下层以模糊控制和积分分离PID控制构建驱动轮滑移控制器,从而实现路径跟踪、运动速度、驱动轮滑移的有效控制,提高了喷雾机在复杂作业环境中的稳定性和路径跟踪精度。采用Adams/Matlab的联合仿真结果表明,在复杂的工况条件下,喷雾机驱动轮的滑移率依然控制在±20%之内,防止驱动轮发生过度滑移对车速和转向角产生不良影响,有利于喷雾机稳定性的提升。本喷雾机能够快速准确地跟踪期望路径,与未考虑驱动轮滑移的控制相比,能够适应更加复杂的工作环境,跟踪精度有明显提升。     

双季超级稻强源活库优米栽培技术的示范推广

结合兴宁市双季超级稻生产的实践,对其强源活库优米栽培技术进行简单的总结,包括结合双季超级稻品种的特性适时与适密抛(插)秧、节水灌溉、使用超级稻专用肥、病虫害防治等,并分析了该技术示范推广后的效果。     

Functional characteristics of porcine peripheral T cells stimulated with IL-2 or IL-2 and PMA

In human or mouse, mature T cells express either CD4 or CD8, resulting in different functions in the periphery. Interestingly, porcine CD4 and CD8 double positive (DP) T cells are present in the blood, and their proportions change from youth to adulthood. However, the features of these cells in swine are poorly understood. We investigated the fate of porcine peripheral T cells based on their functional characteristics, including proliferation and the expression of CD4 and CD8 co-receptors. The results showed that all the populations changed their CD8 expression in a time-dependent manner and porcine T cells had different proliferative pattern from human T cells. The results further revealed that Th2 cytokines were increased later in porcine T cells compared to human T cells upon stimulation with IL-2 + PMA. Collectively, we found that the fate of porcine peripheral T cells is different from that of human T cells, and the changes occur in a time- and stimulation-dependent manner.     

鱼鳞坑开沟双犁体翻转机构研究设计

双犁体翻转机构是对已有的发明专利机型即鱼鳞坑开沟犁装置的进一步优化设计,解决了在单犁体鱼鳞坑开沟犁返程时因开沟翻土方向不在坡下而是在开出的沟上面的作业问题。新研制翻转式鱼鳞坑开沟工作结构装置,应用机械、液压、电控装置实现了不同土壤类型的鱼鳞坑开沟间距式作业,生产效率高,性能可靠,市场前景广阔。     

Fishing gear effects and ecology of the sea whip (Halipteris willemoesi (Cnidaria: Octocorallia: Pennatulacea)) in British Columbia,Canada: preliminary observations

• 1. We conducted a preliminary study of the effects of a shrimp beam trawl and prawn traps on sea whips (Halipteris willemoesi (Cnidaria: Octocorallia: Pennatulacea)) at two bays on Clio Channel, south central coast of British Columbia, Canada. Video and grabs surveys were conducted to estimate abundance and make observations on sea whips and their habitat.
• 2. No adult sea whips were caught in six beam trawls. In 600 prawn (Pandalus platyceros) trap sets at Turnour Bay, 30 sea whips were found entangled in the gear and of these 50% of the colonies were damaged. The length of broken sea whips ranged from 0.09 to 1.03 m. At Turnour Bay, the density of adults observed in video surveys ranged from 7.1 to 14.3 m^?2. Juvenile density in grab samples ranged from 53 to 123 m^?2 and a few adults were also caught. The lengths of intact adult sea whips caught in traps and grabs ranged from 0.20 to 1.98 m and juveniles from 0.3 to 0.7 cm.
• 3. Fewer adult sea whips (<0.1 m^?2) were seen in Bones Bay, where sediments were muddier (grain size <0.25 mm: 84.6 to 97.2%) relative to Turnour Bay (grain size <0.25 mm: 71.4 to 85.9%). Bottom water characteristics at the two bays were similar. Seasonal temperature range was 7.5–8.4°C, salinity 30.63–31.49 psu, and dissolved oxygen 2.76–5.97 mg L^?1. Differences in sediment characteristics and food availability may have been a factor influencing differences in abundance of sea whips between the two bays.
• 4. Further data are needed to investigate the effects of trawling and trapping on sea whips, as sample sizes were small in the present study and only two gear types were evaluated. Studies of effects of otter trawling and heavier traps, such as those used for Dungeness crab (Cancer magister), are warranted.

Copyright © 2005 John Wiley & Sons, Ltd.     

H1N1亚型猪流感病毒HA和NA基因双重RT-PCR定型检测方法的建立与应用

为了建立适用于临床诊断的H1N1亚型猪流感病毒快速检测方法,本研究根据GenBank已登录的H1N1亚型猪流感病毒HA和NA基因序列设计RT-PCR扩增引物,以H1N1亚型猪流感病毒、H3N2亚型猪流感病毒、猪瘟病毒和猪繁殖与呼吸综合征病毒为试验对照,通过优化RT-PCR反应条件和反应体系,建立了H1N1亚型猪流感病毒HA和NA基因双重RT-PCR定型检测方法。同时,运用H1N1亚型猪流感病毒血凝和血凝抑制试验方法和本研究建立的方法对165份猪病料样品进行了对比验证。结果表明,本研究建立的H1N1亚型猪流感病毒双重RT-PCR具有良好的特异性、敏感性、重复性,所扩增的目的基因片段大小分别为428 bp和678 bp左右,可检出最小基因组RNA浓度为2.9×10-5μg/μL。本研究建立的方法和H1N1亚型猪流感病毒血凝和血凝抑制试验方法均从同一份猪肺脏样品中检测出H1N1亚型猪流感病毒,其余样品中均未检出H1N1亚型猪流感病毒,两种方法符合率为100%。本研究建立的方法适用于H1N1亚型猪流感病毒双基因定型检测,可在H1N1亚型猪流感病毒流行病学调查和临床诊断中应用。     

深水抗风浪网箱监测系统研制方案的探讨

探讨一种声学监测方案,用高频、窄脉冲和分裂波束技术,实时监测养殖鱼的进食、生长情况,随时评估网箱内总量的增长情况,并且从水产行业的成本可承受性的实际出发,将成本控制在用户可接受的水平上。     

中华绒螯蟹副性征的研究

在解剖、测量性成熟和未成熟中华绒螯蟹外部形态和性腺之基础上,试用“双百分率法”和“差异显著性检测法”对中华绒螯蟹的副性征进行了研究。结果表明,性成熟后的雌、雄中华绒螯蟹均具有较明显的副性征。