Metallothionein inhibits homocysteine-induced proliferation of rat vascular smooth muscle cells

AIM:To investigate the effect of metallothionein(MT) on proliferation of rat vascular smooth muscle cells (VSMCs) stimulated by homocysteine and its mechanism. METHODS:VSMCs proliferation was measured by [³-H]-TdR incorporation, mitogen-activated protein kinase(MAPK)activity were determined by immunoprecipitation method, the intracellular contents of MT and malondialdehyde (MDA)were assayed by -hemoglobin saturation method and TBA reaction, respectively, and lactate dehydrogenase (LDH) leakage was measured by NADH oxidation. RESULTS:Hcy(10^-6-10^-4 mmol/L) stimulated [³-H]-TdR incorporation by the VSMCs in a concentration-dependent manner. Compared with control, [³-H]-TdR incorporation in VSMCs treated with 0.1 mmol/L Hcy was increased by 4.2 fold (P＜0.01). Meanwhile, Hcy enhanced MAPK activity, MDA formation and LDH release (P＜0.01)in a concentration-dependent manner. Treatment of VSMCs with MT alone did not change above parameters, compared with control. However, MT (10^-6-10^-4 mol/L)attenuated significantly Hcy-stimulated proliferation of VSMCs (P＜0.01)in a concentration-dependent manner. And MT inhibited obviously Hcy-induced activation of MAPK activity, MDA formation and LDH release. Preincubation of VSMCs with 0.5 mmol/L ZnCl₂ for 6 h induced an increase cellular MT content by 5.7-fold (P＜0.01). The MT-overexpressed VSMCs resisted Hcy-stimulating action on MAPK activity, MDA formation and LDH leakage (P＜0.01). CONCLUSION:These results show that MT has an inhibitory effect on Hcy-induced VSMCs proliferation, and that MT could inhibit Hcy-stimulated MAPK activity and lipid peroxidation.     

Effect of Jiawei Sini Decoction on glucocorticoid receptor in thymocytes of chronic psychological stress rats

AIM: To observe the effect of Jiawei Sini Decoction (JWSND) on glucocorticoid receptor (GCR) in thymocytes of chronic psychological stress rats. METHODS: The rats were randomly divided into 4 groups, control group (C), model group (M), group treated by JWSND C₁, group treated by ginsenosides C₂. The number of thymocyte GCR sites and the GCR nuclear thanslocation rate were detected by radioimmunoassay. RESULTS: Compared with the control group, in the model group, the thymocyte weight index lowered significantly ( P<0.05 ), and the GCR nuclear thanslocation rate was increased significantly ( P<0.01 ), but the number of thymocyte GCR sites was unchanged. Compared with the model group, thymus gland weight indexes of C₁ and C₂ were increased significantly ( P<0.05 ), while the GCR nuclear thanslocation rate lowered significantly ( P<0.01 ). Moreover, no significant difference was found in all indexes between C₁ and control group. CONCLUSION: The inhibitory effect of glucocorticoid on the thymus could be significantly reversed by JWSND via suppressing the thanslocation of GCR from cytoplasm to nucleus in chronic psychological stress rats.     

Change of central noradrenergic system in Alzheimer's disease

The abnormality of the central noradrenergic system in Alzheimer's disease has two cases: decrease or increase. The former is easy to be understood, because it is resulted from the outstanding forfeit of the noradrenergic neurons in the nucleus locus ceruleus (nLC); but the noradrenalin concentration does not change and enen increase, it seems to disagree with the loss of the noradrenergic neurons in the nLC. This article put emphasis on it, enumerated the related evidence and analyzed the possible causes. At present time, the drugs to increase noradrenalin is used mostly in clinic. This paper summarized it and put forth our own opinions.     

典型光活化毒素α-三噻吩对棉铃虫和亚洲玉米螟Na~+-K~+-ATPase的抑制作用

以棉铃虫 H elicoverpa armigera和亚洲玉米螟 Ostrinia furnacalis为试虫 ,建立 Na+- K+-ATPase最佳反应系统 ,研究典型光活化毒素 α-三噻吩 (简称 α- T)对 Na+- K+- ATPase的影响。棉铃虫 Na+- K+- ATPase活力最佳测试条件为酶源蛋白浓度 6μg/ m L ,反应温度 35～ 4 0℃ ,反应时间6min;亚洲玉米螟则为酶源蛋白浓度 8μg/ m L,反应温度 35℃ ,反应时间 6min。近紫外光照 (30 0～4 0 0 nm )对棉铃虫和亚洲玉米螟离体 Na+- K+- ATPase活力基本没有影响 ,但对活体活力有很强的抑制作用。光照和无光照条件下 ,α- T对两种昆虫离体和活体 Na+- K+- ATPase活力均有不同程度的抑制作用。光照组 α- T对亚洲玉米螟 Na+- K+- ATPase的抑制率高于无光照组 ,且处理浓度或剂量越高 ,其抑制率越大 ;对棉铃虫 Na+- K+- ATPase抑制作用不显著     

中生菌素对水稻白叶枯病的防治机制

对不同白叶枯病抗性水稻品种用200μg/ml中生菌素55℃温汤药液浸种，自然降温，秧田3—4叶期和移栽前5d各用30μg/ml中生菌素处理后，于成株期剪叶接种白叶枯病菌。结果表明中生菌素前期处理，于成株期接种白叶枯病菌时，高抗、中等抗性和感病品种中过氧化物酶、苯丙氨酸解氨酶和多酚氧化酶3种酶活性都有不同程度的提高，且以中等抗性品种酶活提高最多，接种24和48h，3种酶活性较清水对照分别增加26．92％、26．74％、24．06％和7．09％、1．31％、1．60％。盆栽试验表明，中生菌素对中抗品种的防治效果最好，达58．4％。说明中生菌素对水稻防御酶活性的诱激作用是其防治白叶枯病的机制之一。     

阿魏蘑与杏鲍菇高产优质栽培模式研究

试验证明，阿魏蘑和杏鲍菇最佳栽培模式为菌棒半脱袋竖向畦栽半覆土栽培模式；最适环境条件为菇房温度13～20℃，空气相对湿度85％～95％；最佳栽培设施为半地下冬暖式塑料大棚。     

黑龙江省城乡养殖专业户饲养奶牛的成本收益对比分析

大中城市郊区奶牛饲养作为畜产品匮乏时代的产物，作为国家“菜篮子工程”的重要组成部分，自然有其存在的历史合理性，但由此造成的日益严重的环境污染也不容忽视。以哈尔滨市为代表的黑龙江省大中城市专业户奶牛饲养，与农村专业户奶牛饲养相比，在生产成本、产品产量、收益水平等方面均不具有竞争优势。作为奶牛饲养基地布局，应将重点从大中城市郊区向远郊县区有条件的农村转移。     

纳米微粒材料生物学活性研究进展

综述了纳米微粒的生物学效应及其免疫调节、抗菌、抗肿瘤等多种生物学活性.     

不同培养条件对鲍鱼菇菌丝生长的影响

通过不同温度、pH值及培养基含水量处理 ,对鲍鱼菇菌丝生长状况进行了研究。结果表明鲍鱼菇菌丝生长适宜的温度范围为 2 0～ 31℃ ,最适温度为 2 5～2 8℃ ;pH值适宜的范围为 4 0～ 7 0 ,最适pH值为 5 5～6 0 ;培养基含水量适宜的范围为 6 0 %～ 80 % ,最适含水量为 80 %。     

不同培养基对杏鲍菇菌丝生长的影响

比较了杏鲍菇一级种和二级种培养基配方 ,试验表明杏鲍菇一级种培养基以配方三最好 ,菌丝干重是配方七 (对照 )的 3 5倍 ,差异极显著 ;杏鲍菇二级种培养基以配方四最好 ,菌丝生长势最强 ,与供试各培养基配方相比较 ,差异均为极显著