内蒙古桦木沟林场不同地貌野生花卉资源分布

内蒙古桦木沟林场地形地貌类型广泛,野花资源丰富,尚未得到有效开发。为了解野生花卉资源习性和生物学特征,开发利用野花资源,在路径调查的基础上,采用典型样地调查法,调查分析了其野花资源分布。共调查野花资源177种,隶属于40科129属,菊科、豆科、毛茛科等种类和种群数量较丰富。不同地貌Margalef丰富度指数1.35~8.28,Shannon-Wiener指数变异范围1.42~2.59,Simpson指数为0.59~0.96,湿地草甸、疏林草地物种多样性丰富,沙地多样性最低。湿度、光照等是影响野花多样性分布的重要因素。林场分布着丰富而有较大开发价值的喜光、耐荫、耐涝、喜湿、耐旱等不同生态型野生花卉。部分野生花卉资源濒危,亟需保护。     

不同密度紧凑型油菜的源库特征及与收获指数的相关研究

旨在为油菜株型育种和机械化栽培提供理论和技术依据。选用3个不同类型的紧凑型油菜常规品种,以松散型油菜杂交种‘秦优7号’为对照,研究了在15万、30万、45万、60万、75万、90万株/hm²密度下各品系的库源特征及其与收获指数的关系。结果表明,紧凑型油菜单株在不同密度下的库源特征与对照松散型相同,单株根、茎、枝、壳干重,角粒数和单株角果数随种植密度的增加而降低,且低密度显著高于高密度;千粒重随密度的变化不大。紧凑型油菜的生物学产量和经济产量随种植密度的增加而增加,松散型油菜随种植密度的增加而下降。说明紧凑型油菜适宜密植和机械化收获。收获指数对密度的响应在两类型品种表现一致,最低密度15万株/hm²增加到30万株/hm²时达到峰值,以后随着种植密度的继续增加而降低。收获指数与茎+枝+壳干重之和,与库性状角粒数、角果数、千粒重、单株产量,与库源关系的粒根比、粒茎比、粒枝比、粒壳比显著正相关,说明要提高收获指数不仅要扩“源”增“库”,还要协调库源的合理分配比例。     

异噻菌胺原药高效液相色谱分析方法研究

本文采用高效液相色谱法,以乙腈+磷酸溶液为流动相,使用ZORBAX SB-C18色谱柱和二极管阵列检测器,在280nm波长下对异噻菌胺原药进行分离和定量分析。结果表明,该分析方法的线性相关系数为1.000 0,标准偏差为0.39,变异系数为0.39%,平均回收率为100.08%。     

Infection of human enterovirus 71 in immunosuppressed rhesus monkeys

AIM: To investigate the infection process of human enterovirus 71 (EV71) in a rhesus monkey model of immunosuppression.METHODS: The monkey immunosuppression model was established by oral administration of cyclosporin A at a daily dose of 15 mg/kg for 7 days. Fourteen days after inoculation of EV71 FY23 strain via respiratory and digestive tracts, the infectious monkeys were sacrificed, and then the pathological changes of systemic organs were observed and the viral analysis in different tissues was performed.RESULTS: Compared with the experimental group without cyclosporin A, more positive results were observed in the immunosuppressed monkeys, such as more severe clinical symptoms, higher viral load/antigen expression and more pathological changes in the organs.CONCLUSION: The findings of the viral multiplication in the host provide evidence for evaluation of EV71 vaccine, and also indicate the delimitation for the population using EV71 vaccine in future.     

Nutrient cycling and biomass flows in a low-quality forest stand improvement system in the Lesser Khingan Range of China

The material flow and bulk internal flow analyses were used to establish a material accumulation and cycling model for a low-quality forest stand improvement system and a series of processes were considered. The model was applied in a one-hectare low-quality forest plot in the Lesser Khingan Range of China. Results showed that during 1997–2007, the stands absorbed 270.19 kg of N, 74.28 kg of P, and 124.39 kg of K from soils, 51.82 kg of N and 2.38 kg of P were directly absorbed by foliage, and 16.25 kg of K was released to soils by eluviation. Until 2007, the accumulated nutrients in the stands included 236.91 kg of N, 65.28 kg of P, and 108.55 kg of K. When horizontal strip clearcutting was applied in 2007, 50% accumulated nutrients in the stands were shifted due to harvesting operations, and 212.74 kg of N, 26.97 kg of P, and 98.88 kg of K were accumulated in soils, declining by 9.47% for N, 3.68% for P, and 17.60% for K, respectively, compared with year 1997. 94.61 t per hectare of biomass was generated, of which the biomass in stands accounted for 87.36%. The felled tree biomass was 36.89 t per hectare, of which 84.90% and 10.03% of biomass were utilized in terms of logs and other means, and the rest was left on site.     

菜籽油实罐储存品质变化规律研究

通过对2010~2014年大、中、小不同罐容实罐储存的四级菜籽油,采取定点、定罐、定层、定期跟踪观察油罐"三温"变化并取样检测,发现罐温、油温均随着气温的变化而变化,其中罐温最高,油温从上至下依次降低。菜籽油的水分及挥发物、色泽、气味、滋味、加热试验(280℃)等指标在储藏期间变化均较平稳,酸值和过氧化值均呈不断上升趋势,且不同年限的菜籽油其过氧化值上升幅度明显高于酸值。油罐容量越大,满罐存储时其存油品质的稳定性越好。罐内上、中、下层酸值变化差异不明显,上层和底层的过氧化值明显高于中层。严格控制好入罐油品质量,菜籽油储藏4~5年后酸值、过氧化值等质量指标仍可保持在国家限量标准以内。     

遵义市辣椒产业现状及发展战略

遵义市是中国辣椒之都,位于全国最大的辣椒主产区西南和华中的中心区域,也是全国辣椒消费重辣区,其交通便捷,环境优美,辣椒产业文化丰富。当前遵义辣椒产业正处在基地优化、技术升级和产业提升的关键时期,亟需理清发展思路。通过对遵义辣椒产业现状的阐述与问题的分析,从而针对性地提出了发挥种植优势、突出产品特色,优化产区布局、推广生态化栽培,培育大型加工企业、促进加工产业升级与转型等战略,以促进遵义辣椒产业的发展。     

Study on Anti-viral Effect of Rhubarb on Porcine Epidemic Diarrhea Virus in vitro

The inhibitory effects of rhubarb on cell lesion, which was caused by porcine epidemic diarrhea virus (PEDV), and its mechanism had been studied in this research.The maximum safe concentration of rhubarb solution applied to Vero cells was determined through morphological observation, and then the PEDV infected Vero cell model in vitro was established, by which the inhibitory effect of rhubarb on virus was studied.The experimental method had also been established based on this cell model.The result showed that the maximum safe concentration was 3.28 mg/mL.Both morphological observation and MTT assay were indicated that rhubarb could not only block the attachment of the virus to Vero cell and inhibit the synthesis and reproduction of the virus, but also could directly inactivate the virus.This research showed that rhubarb had an inhibitory effect on PEDV, and provided a theoretical basis for the prevention and treatment of clinical porcine epidemic diarrhea.     

苹果‘长富2号’开花调控转录因子基因MdIDD7的克隆及表达分析

以‘长富2号’苹果为试验材料,采用RT-PCR的方法,从其芽中克隆得到INDETERMINATE DOMAIN(IDD)转录因子基因MdIDD7,其开放阅读框长度为1 626 bp,编码541个氨基酸。序列比对和结构域分析表明,该转录因子含有1个核定位信号和4个高度保守的锌指蛋白结构域。系统进化树分析表明,MdIDD7与白梨(Pyrus×bretschneideri,XP_009364602.1)、桃(Prunus persica,XP_007225628.1)和梅(Prunus mume,XP_008220893.1)聚在一起。实时荧光定量PCR表明,MdIDD7在‘长富2号’不同组织(茎、叶、花、果和芽)中均有表达,其中芽的表达量最高。花后40~60 d,MdIDD7在易成花品种‘烟富6号’芽中表达量显著高于难成花品种‘长富2号’。"小年"树顶芽组织中MdIDD7的表达量显著高于"大年"树。在花芽诱导前期,外源GA处理诱导MdIDD7下调表达,而蔗糖处理诱导其上调表达,说明MdIDD7响应激素和糖信号,促进苹果成花。     

钾肥减量分期施用对莴笋产量和品质的效应研究

通过大田试验研究了紫色菜园土壤钾肥减量分期施用对莴笋产量和品质的影响。结果表明：两个试验点钾肥减量10%~30%分期施用对莴笋产量有提高作用，各处理以K2（钾肥减量10%分期等量追施）效应最好，增产最大（4.2%和4.5%）、施肥利润最高（84923元/hm²和72075元/hm²）。两个试验点莴笋叶维生素C含量均呈下降趋势，K2处理较常规施肥(CK)处理均显著降低莴笋叶维生素C含量（8.0%和8.7%）；而莴笋茎维生素C含量变化规律不一致。钾肥减量分期施用降低两个试验点莴笋叶和茎硝酸盐含量，以K4处理（钾肥减量30%分期等量追施）降低作用最大；从莴笋叶和茎糖组分的均衡性看，以K3处理最好。钾肥减量分期施用对两个试验点莴笋叶必需氨基酸含量有显著增加作用，改善叶氨基酸品质性状，但降低莴笋茎必需氨基酸含量和品质性状；莴笋氨基酸品质性状以K4处理为最优，其次是K2处理。综合考虑莴笋产量和品质，紫色菜园土壤以K2处理（钾肥减量10%分期等量追施）最优。