鸡ADSL基因和GARS-AIRS-GART基因对鸡肉肌苷酸(IMP)含量的影响

以鸡ADSL基因、GARS-AIRS-GART基因为侯选基因,以隐性白羽鸡、丝羽乌骨鸡、萧山鸡、白耳鸡、藏鸡为试验材料,采用PCR-SSCP方法对ADSL外显子2、GARS-AIRS-GART基因5′侧翼序列进行SNPs检测。结果各发现了一个单核苷酸多态性位点。各种基因型与胸肌IMP含量的最小二乘分析结果表明:ADSL基因TT型个体的胸肌肌苷酸含量极显著地高于CC型、显著地高于CT型个体,CT型个体也高于CC型,但差异不显著;GARS-AIRS-GART基因TT型个体的胸肌肌苷酸含量极显著地高于CT型和CC型个体,CT型个体也高于CC型,但差异不显著。ADSL和GARS-AIRS-GART基因的合并基因型对IMP含量也有显著的影响,合并基因型为TTTT的个体IMP含量显著高于CCCC合并基因型的个体,高出1.584 mg/g,因此可以利用该合并基因型对鸡的肉质风味性状进行标记辅助选择。     

地下长廊蜜蜂越冬技术的研究

依据蜜蜂室内外越冬原理和蜜蜂生物学特性,按养蜂生产的需要,研究设计了一种蜜蜂越冬的地下长廊,两侧放置1～3层蜂箱,中间为通道,上部封闭,留有进出气孔调节温度,人可以进入长廊管理越冬蜂群.挖建长廊的成本低,越冬期温度稳定,越冬蜂群势削弱率降低20.1%～24.3%,饲料消耗量降低10.5%～16.2%.     

公鸡和母鸡氮代谢及氮维持需要量的比较研究

试验采用氮平衡试验研究产蛋高峰期罗曼公、母鸡的氮维持需要量(Nm),并分析公、母鸡氮代谢的变化规律。试验选取27周龄健康的公、母鸡各30只,分别分为5个处理,每处理6个重复,每个重复1只试鸡,对公、母鸡分别饲喂5个不同粗蛋白质水平的试验日粮。结果表明:相同蛋白水平处理组的公鸡氮食入量和氮沉积率均高于母鸡的结果。母鸡和公鸡的氮食入量(NI)与氮沉积量(NB)间的回归方程分别为NB=0.3743NI-0.1589(R2=0.79)和NB=0.6228NI-0.3151(R2=0.85)。母鸡和公鸡的氮维持需要量分别为每天0.4245g和0.5059g;每千克体重0.2364g和0.2754g;每千克代谢体重0.2739g和0.3208g。     

梅花鹿巴氏杆菌病的诊治

2006年7月14日,临夏州某养殖场饲养的79只梅花鹿鹿群中个别发生一种以咳嗽、呼吸困难、流粘脓性鼻液为主要特征的疫病.发病8只,发病率为10.13%;死亡2只,死亡率为25%,不分年龄大小均发病,但以幼鹿和体弱者为主.     

香蕉A基因组品种间遗传关系的SSR检测

应用SSR技术,对32个香蕉A基因组类型品种(系)的遗传关系进行了检测。40对SSR引物在32个品种(系)中分别扩增带数在3～15个,平均每个SSR座位可检测2.99个多态性带;引物的多态信息量(PIC)在0.00～0.88,平均0.62。依据SSR数据计算的品种间遗传距离在0.00%～34.27%,平均12.45%,大多数品种间的遗传变异非常有限,但也存在着遗传差异突出的品种:FHIA25、Yangambi KM5、Pisang Jari Buaya、Rose和皇帝蕉。依据26%的遗传距离,除了FHIA25和Pisang Jari Buaya单独化成1组外,其它30个品种可以分为2组:品种间遗传差异相对较高的组I和品种间遗传差异相对较低的组Ⅱ。Williams与引进的洪都拉斯3号、M931之间,洪都拉斯1号和洪都拉斯2号之间,高脚青芽蕉和高脚顿地雷分别没有区分开来,这可能是同物异名,也可能是同一品种未能分辨的突变体。     

VEGF induces HUVECs to produce extracellular H2O2 and its proliferation role

AIM:To study the effect of VEGF on extracellular H₂O₂ production in HUVECs and the role of H₂O₂ in the VEGF-induced proliferation. METHODS:HUVECs was stimulated with 500 μg/L VEGF. Products of extracellular H₂O₂ was detected by H₂DCFDA staining. MTT method was used to value the influences of 3×10⁶ U/L catalase and 5-20 mmol/L H₂O₂ to VEGF function. RESULTS:After treatment for 15 min with VEGF, HUVECs appeared fluorescence, and continued to become stronger, peaked at 45 min then decreased. HUVECs, which was treated simultaneity with VEGF and 3×10⁶ U/L catalase, only appeared very faint fluorescence. The proliferation of HUVECs by VEGF was restrained when treated with 3×10⁶ U/L catalase. The extrinsic H₂O₂ at concentration of 5-10 mmol/L promoted the proliferation of HUVECs but inhibited the proliferation effect of VEGF on HUVECs (P<0.01). CONCLUSION:These findings indicate that VEGF induces HUVECs to produce extracellular H₂O₂ and plays role in proliferation, but extrinsic H₂O₂ restrains VEGF function.     

Effects of PDS on rat brain cortical nuclear factor kappa B in LPS shock

AIM:To explore the molecular mechanism of brain tissue injury induced by lipopolysaccharide (LPS),the effects of panaxadiol (PDS) on the expression of nuclear factor kappa B (NF-κB) in cerebral cortex of rat with LPS shock were studied. METHODS:Rats were randomly divided into LPS roup,LPS+dexamethasone group,LPS+PDS group and control group. The DNA binding activity and protein expression of NF-κB were observed. These indices were assayed at 1 h and 4 h after intravenous injection of LPS (4 mg·kg^-1). RESULTS:EMSA showed that PDS inhibited NF-κB DNA-binding activity in nuclear extracts at both 1 h and 4 h after LPS injection,compared with the LPS group (P<0.01). Western blotting showed that PDS down-regulated the expression of p65 and p50 protein in the nuclear extracts compared with the LPS group. However,the expression of p65 and p50 protein from cytosolic extracts did not show any significant change. CONCLUSION:PDS may alleviate brain injury by inhibiting NF-κB activation.     

木豆在干热河谷退化山地的生态适应性研究

2000年木豆(Cajanus cajan(L.)Millspaugh)引入云南省元谋干热河谷地区,观测其物候特征、生长适应性、抗旱适应性、抗光热适应性等植物学指标,以了解其对干热退化山地的整体生态适应性.结果表明,木豆能适应干热河谷的退化山地生长环境,其生长势优于原产地,表现出明显的速生性;木豆的抗光热能力较强,能适应于热河谷的不同光照及高温环境,但温度高于37℃,则光合作用停止;木豆的抗旱能力较强,能够通过不同的生理适应性度过旱季,完成其生长周期.木豆适应于干热河谷的生态环境,利用价值较大,是干热河谷退化山地治理与开发的优选灌木.     

夏季覆盖盐碱地表面对土壤盐分和水分的影响

在干旱区土壤水分蒸发损失引起土壤盐分表聚是土壤次生盐碱化的一个重要原因.在夏季高温时节,采用塑料薄膜、麦草和沙子覆盖盐碱地表面,结果表明,具有较好地改良盐碱地的作用.塑料薄膜宜采用全地面覆盖,麦草的覆盖量应为2 934.8 kg/hm2;沙子的覆盖厚度以4 cm为宜.覆盖后对土壤中各离子的抑制作用顺序为:K Na ＞ Cl-＞ SO42-＞Mg2 ＞HCO3-＞Ca2 .3种覆盖材料以塑料薄膜效果最佳.