猪肌生成抑制素在COS-7细胞中的表达及检测

本研究室构建的猪肌生成抑制素(MSTN)成熟蛋白编码序列的真核表达质粒pef-dhfr1a-MSTN,转染到COS-7细胞中,使猪MSTN成熟蛋白编码序列全长358个氨基酸在COS-7细胞中进行表达,利用Trizol试剂提取转染细胞的总RNA,借助RT-PCR和SDS-PAGE电泳方法,分别从mRNA水平和蛋白质水平上检测到了猪MSTN在COS-7细胞中的表达,并应用Western-blotting方法证实了猪MSTN表达的特异性。     

猪群HEV血清抗体调查及一株新的猪HEV ORF2部分基因序列分析

戊型肝炎病毒(Hepatitis E virus,HEV)主要引起人的戊型肝炎,新近研究发现猪在病毒传播中可能发挥重要作用.本研究对我国部分省区HEV感染血清学调查,在被检的1 138份血清中,有666份(57.5%)为HEV抗体阳性,猪群抗体阳性率随着月龄增长而升高.通过RT-PCR方法从一份猪粪中扩增并克隆了HEVORF2 N端主要抗原决定区339bp基因片段,序列分析显示,该段基因与我国人群HEV基因4型毒株核苷酸序列同源性为85.9%,但氨基酸序列完全一致.这一结果提示我国猪群存在广泛的HEV感染,并在一定程度上与人群HEV毒株密切相关.     

F18ac菌毛A亚单位基因的克隆与序列分析

根据已经发表的F18ab菌毛A亚单位(FedA/ab)的基因(fedA/ab)[1],设计一对引物,利用PCR技术从表达F18ac菌毛的大肠杆菌2134P株[2]、8199株[3]、8813株[3]中分别扩增到一段序列,并克隆至pGEM-T载体,获得重组质粒T2134PA、T8199A、T8813A.琼脂糖凝胶电泳、序列测定及分析表明,该3个序列大小均为516bp,与fedA/ab(513bp)具有较高的同源性,分别为96.3%、96.5%、95.9%,推导的Fed/ac氨基酸序列与FedA/ab同源性分别为93.0%、93.6%、92.4%.数据表明该实验所克隆的序列均为F18ac菌毛A亚单位(FedA/ac)的基因(fedA/ac).     

Effects of antibiotic treatment on the inflammatory mediator expression in peritoneum and the peritoneal transport function in the rat with acute bacterial peritonitis

AIM: To observe the effect of antibiotic treatment on the inflammatory mediator expression in peritoneum and the peritoneal transport function in rats with acute peritonitis, and explore its mechanisms. METHODS: Eighty-six SD rats were randomly divided into three groups. Control group (n=28) were treated with PBS (ip), peritonitis group (n=28) and treatment group (n=28) were challenged with the E.coli (ip), but at 3 h and 9 h gentamicin was given (ip) in treatment group. Seven rats of every group were randomly sacrificed at 24 h, 48 h, 72 h and 7 d. Peritoneal equilibration test (PET) was did before they were killed. Leukocyte count, pathological changes and the expression of CD45, NF-κB， IL-1β， TNF-α in peritoneum were examined. RESULTS: (1)The blood leukocytes in peritonitis group decreased strikingly, but did not change obviously in other two groups. The peritoneal fluid leukocytes in peritonitis group increased significantly from 24 h to 72 h, while in treatment group, it enhanced more strikingly than peritonitis group at 24 h, and recovered earlier. (2) Both in peritonitis group and treatment group, the expression of activated NF-κB, IL-1β, TNF-α and CD45 increased significantly, but the treatment group was lower than model group at 48 h and 72 h. The mRNA level of IL-1β and TNF-α had the same trend as their protein expression. (3) Compared with the control group, UF and D/D0 Glu decreased significantly in model group and treatment group, and D/PTP increased dramatically. The D/P TP in treatment group lowered obviously compared with peritonitis group, while the net UF and D/D0 Glu had not significant difference between treatment group and model group. CONCLUSION: Antibiotic treatment can partly decrease the expression of inflammatory mediators in peritoneum of rats with acute peritonitis and also can improve the protein transport ability to some extent, but can not improve the peritoneal ultrafiltration and the glucose transport function.     

硒对硒蛋白-谷胱甘肽过氧化物酶基因表达及其酶活性的调节

本文根据国内外研究现状详细综述了硒调节细胞型谷胱甘肽过氧化物酶(GPX1)mRNA水平、蛋白水平及酶活3个水平的可能分子机理,初步概述硒对其余GPX基因表达的影响及其作用机制,为深入研究硒调节GPX基因表达的作用机制提供参考。     

内蒙古旅游业发展的实践分析及总体评价

内蒙古自治区的旅游业经过多年的培育发展取得初步成绩,按照旅游地生命周期理论其发展实践历程基本经历了探索阶段、初步增长阶段,刚刚步入发展阶段,开始了规模扩张。在这个阶段,内蒙古旅游业的发展还存在提高对国民经济的贡献度、深度开发旅游资源、完善产业链条、优化行业结构、提升旅游企业竞争能力等诸多问题。需要优化旅游空间与布局;聚集具有创新能力的企业群体和企业家群体;深入旅游发展研究,重视科研队伍培养;进行营销策划,树立良好形象;加强生态保护与建设。     

内蒙古入境旅游市场结构研究

旅游业的发展离不开客源市场。通过对内蒙古入境旅游市场的年际变化、地域构成、境内分布情况和消费构成的分析,阐明了内蒙古入境旅游市场的基本特点。提出了确定市场重点,定位客源结构;合理进行空间布局,扩大入境游客在自治区境内的流向;深度开发旅游资源,打造名牌产品;优化旅游消费结构等进一步开拓入境旅游市场的建议。     

紫花苜蓿品种比较试验

通过对26个紫花苜蓿（Medicago sativa）品种的生育期、株高、产量、越冬率等指标的试验比较，结果表明：公农一号、保丰、陇东、德福等几个品种的表现优于其他参试品种，适宜在辽西北风沙地地区推广种植。     

铬对肉仔鸡生长性能、免疫功能和胴体品质的影响

用21日龄Arbor Acress(AA)肉公鸡336只,研究饲粮中添加铬对肉仔鸡生长性能、免疫功能和胴体品质的影响。将试验鸡只共分为8个处理组,分别添加源于氯化铬的铬2．0、4．0、6．0、8.0、10．0、12．0和14．0mg/kg。结果表明:饲粮中添加2．0mg/kg铬显著提高了肉仔鸡胸腺重(P<0．10),降低了腹脂率(P<0．10),4．0、6．0mg/kg铬显著提高了全净膛率(P< 0．10),各试验组显著提高了T淋巴细胞转化率(P<0．10);饲粮中添加铬对其余指标则无显著影响(P>0．10)。     

大兴安岭高寒地区滑菇代料栽培技术

通过多年实践总结出一套适合本地的滑菇种植技术．包括菌种选择、培养料的制作、装袋、灭菌、接种、培养、出菇管理、采收全过程栽培技术．以期促进林区滑菇生产尽快发展．使大兴安岭地区成为滑菇夏季出菇基地．