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101.
Cui Xudong Su Xiaohua Zhang Bingyu Chu Yanguang Huang Qinjun Zhang Weixi Liu Boyang 《中国林业科技(英文版)》2012,(3):54-55
Compared with the species of Leuce section,the difficulty in plant regeneration during tissue culture for the species of Aigeiros section is a key limiting factor for their application in genetic engineering of forest trees.In this study,the plant growth regulators combination,copper concentration,light intensity,and the selection pressures of kanamycin were investigated using leaves of Populus×euramericana cl.’Bofeng’ as explants,and a stable and high efficient regeneration system was established.Using this system,both the shoot regeneration rate and rooting rate were up to 100%,and the average number of differentiated shoots in each leaf explant was up to 20,and the survival rate of seedlings reached 98%.The optimal selection pressure of kanamycin in shoot inducement for leaf-explant was 40 mg·L-1,and the optimal selection pressure of kanamycin in root inducement of adventitious bud was 20 mg·L-1.The authors also found that differentiation rate of adventitious buds of P.×euramericana cl.’Bofeng’ could be significantly promoted by increased copper concentration,and this is the first report about the key role of copper(Cu) in explant regeneration of Poplars.This regeneration system with high frequency which was established by the authors was as good as that of species of Leuce section,and it provides a good technological platform for genetic engineering of forest tree which uses the species of Aigeiros section as model materials. 相似文献
102.
概述了用于建筑节能的单盘管、双风机的空调(SCTF)在整个建筑模拟程序EnergyPlus中的模拟模型的概念及发展。 相似文献
103.
Youzun Xu Lixiao Nie Roland J. Buresh Jianliang Huang Kehui Cui Bo Xu Weihua Gong Shaobing Peng 《Field Crops Research》2010,115(1):79-84
In double rice-cropping system in China, zero tillage in late-season rice with straw return from early season rice is an emerging technology for saving inputs, shortening the lag time between rice crops, avoiding straw burning, and conserving natural resources. The objective of this 2-year field study was to determine the effects of tillage and straw return on N uptake, grain yield, and N use efficiency of late-season rice. Treatments were arranged in a split-plot design with four combinations of tillage and straw return as main plots and three N management practices as subplots. Tillage was either conventional soil puddling or zero tillage with newly harvested crop residue from early season rice either removed or placed on the soil surface without incorporation. The N treatments were zero-N control, site-specific N management (SSNM), and farmers’ N-fertilizer practice (FFP). Straw return regardless of tillage or N management did not reduce rice yield. In the second year, straw return significantly increased grain yield in the zero-N control. Chlorophyll meter readings at heading and total N uptake at maturity were higher with straw return in the zero-N control, suggesting that straw provides nutrients to rice in the late growing period. Zero tillage did not reduce N uptake, grain yield, and N use efficiency compared with conventional tillage. Despite large differences in timing and rate of N application between FFP and SSNM, these two N treatments resulted in comparable N uptake and grain yield of late-season rice regardless of tillage and straw return. These results suggest that zero tillage after early rice with straw return could replace conventional tillage for late rice in the double rice-cropping system in China. 相似文献
104.
Cang Jing Yu Jing Liu Li-jie Yang Yang Cui Hong Hao Zai-bin Li Zhuo-fu 《东北农业大学学报(英文版)》2012,19(1):27-35
The overwintering survival ratio of the cultivar Dongnongdongmai 1 with strong cold-resistance in paramos of Heilongjiang Province in China are over 85%. The tillering nodes are the most important organs for overwintering survival of winter wheat, because there are more substances associated with cold resistance in tillering nodes than those in leaves and roots. Proteins in the tillering nodes of the cold-resistant cultivar Dongnongdongmai 1 grown under field conditions with or without any low-temperature stress were analyzed by 2–dimensional electrophoresis and identified by mass spectrometry. In the range of pH 4–7, the expression of 37 proteins showed obvious difference (±more than two fold) in the proteomic maps of cold-stressed and non-stressed tillering nodes, including a new protein spot. All proteins exhibiting the difference in expression were identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, followed by a database search for protein identification and function prediction. Five groups of proteins were confirmed, namely stress-related proteins (22%), metabolism-associated proteins (35%), and signaling molecules (24%), cell wall-binding proteins (5%), unclear proteins (14%). This indicated that tillering node cells supported the energy requirements of plant growth and stress resistance by signal transduction adapting to metabolism and structure. 相似文献
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107.
新兽药喹烯酮亚慢性经口毒性研究 总被引:8,自引:1,他引:8
研究了新兽药喹烯酮对大鼠的亚慢性毒性作用.SD大鼠连续6个月经口染毒,剂量分别为32.8、164、820 mg/(kg bw·d),观察染毒期间大鼠一般情况、体重和进食量,于染毒第3和第6个月进行血常规和生化指标检测,取染毒第6个月大鼠的尿分析尿常规指标;染毒结束时处死全部实验动物,计算脏器系数,进行组织病理检查并分析喹烯酮对脏器的损害情况.研究结果表明,820 mg/(kg bw·d)剂量组雄、雌性动物分别有2、3只死亡,且动物的体重明显低于对照组,脑、肺、肝、脾等主要脏器的脏器系数及雄性动物总蛋白、白蛋白含量均显著高于对照组,病理组织学检查发现肝胆管增生;164 mg/(kg bw·d)剂量组雄性大鼠肝、脾、睾丸和雌性大鼠脑、心、肝脏的脏器系数显著高于对照组.因此,新兽药喹烯酮对大鼠的最大无作用剂量为32.8 mg/(kg bw·d). 相似文献
108.
1牛羊生产稳步快速发展,养殖效益稳定我国肉牛肉羊生产稳步快速发展,养殖效益稳定。据行业统计,2005年前3季度牛存栏14326.37万头,同比增长2.26%;出栏3948.55万头,同比增长6.82%;牛肉产量526.24万t,同比增长6.9%。羊存栏38139.68万只,同比增长3.39%;出栏21451.20万只,同比增长3 相似文献
109.
猪圆环病毒病自1991年加拿大首次报道以来,包括我国在内的世界上许多国家和地区都报道有该病发生。近年来,该病发生呈上升趋势,给世界的养猪业造成了相当大的经济损失。目前猪圆环病毒在猪场中的感染率是很高的,它主要侵害断奶后2-3周的仔猪,发病率为20%。60%,死亡率为5%-35%。已成为阻碍当今养猪业发展的重要病原之一。 相似文献
110.
《动物免疫标识管理办法》由农业部于2002年7月1日颁布实施。我市自实行猪、牛、羊免疫标识制度以来.防疫工作取得了明显成效.进一步加强和规范了动物强制免疫工作。有效控制了重大动物疫病.促进了我市无疫区项目的规范建设和成果巩固。 相似文献