河南省平原区林业生态屏障建设分析与评价

通过对河南省平原区林业生态屏障建设现状的调查,论述了林业生态屏障对平原区的重要影响,同时指出了目前林业生态屏障建设存在的主要问题,以期引起有关部门的关注。     

祁连山林区土壤水分条件的分析与评价

通过对祁连山林区云杉林、圆柏林、灌丛林和牧草地等4种主要植被类型土壤水分动态进行长期定位研究,揭示出生长季节内各植被类型的土壤水分动态变化规律.从土壤水分的变化特征,土壤水分有效性等几个方面对祁连山林区主要植被类型的土壤水分条件进行了分析与评价,表明祁连山林区主要植被类型的土壤水分垂直变化可分为土壤水分弱利用层、土壤水分利用层、土壤水分调节层;林地土壤水分的季节变化可分为土壤水分消耗期、土壤水分补偿期、土壤水分消退期、土壤水分稳定期4个时期.根据土壤水分对植物的有效性,将土壤水分划分为易效水、中效水和难效水三种状态,评价土壤水分有效性认为云杉林水分供应状况最好.     

Effects of NK cells on the etiology of severe pre-eclampsia

AIM: To detect the effects of NK cells on the etiology of severe pre-eclampsia. METHODS: The peripheral blood and umbilical blood from severe pre-eclamptic patients and normal late pregnant women were collected. NK cells were seperated in percoll and counted by SAP. The proliferating ability of NK cells was measured by MTT and killing power of NK cells was determined by ［51Cr］ releasing test. The immunohistochemistry method was used to detect the quantity of NK cells and the expression of HLA-G in placenta of them. RESULTS: (1) Compared with that in normal late pregnant women, the counts of the NK cells in peripheral blood, umbilical blood and decidua of severe pre-eclamptic patients were significantly higher (all P<0.05). (2) Compared with that in normal late pregnant women, proliferating and killing ability of NK cells in peripheral blood and umbilical blood of severe pre-eclamptic patients were significantly higher. (3) The expression of HLA-G protein in placenta of severe pre-eclampsia group was lower than that in normal group (P<0.05). CONCLUSION: The quantity of NK cells in severe pre-eclamptic patients is increased with stronger biologic function. They may be related with the pathogenesis of severe pre-eclampsia.     

新疆三工河流域气候暖湿化特征分析

对三工河流域近40a中山区、近山区、近沙漠区的气温、降水、蒸发资料进行月线性趋势分析，得出流域增温主要在2月和9月。2月增温趋势大于9月。流域春夏季气温多年变化较为稳定。降水增湿在1，2月和夏秋季节，夏秋季节降水增湿趋势大于冬季。整个流域夏季蒸发呈减小趋势，平原区蒸发减小趋势大于中山区。夏季降水增大与蒸发减小有较好的反相关性。流域冬季气候增温增湿显著，夏季增湿作用显著。各地暖湿化在空间和时间上有所差异，中山区相对较弱，平原区较强，近山平原区是气候暖湿化最为显著的地域。     

鸡病毒性关节炎病毒C-98分离株S1基因的克隆与序列分析

提取鸡病毒性关节炎病毒（AVAV）内蒙古分离株（C-98株）总RNA，参考GenBank中禽呼肠孤病毒（ARV）S1133株S1基因序列设计了2对引物，应用RT—PCR技术扩增了病毒S1基因，将S1基因cDNA克隆到pGEM—T Easy载体后测序。将测定序列拼接后与参考毒株ARV-176、ARV-138、ARV-S1133、MDRV—YJL的S1基因序列进行了比较。结果显示，AVAV C-98分离株的S1基因与强毒株ARV-176株的同源性最高，达99．9％；与MDRV—YJL的同源性为99．3％，与弱毒疫苗株ARV—S1133的同源性也达97．9％；与ARV-138株的同源性最低，为81．2％。表明，AVAVC-98株与参考毒株的差异较小，与疫苗株ARV-S1133有很高的同源性。     

ULTRASONOGRAPHIC EVALUATION OF THE EXTERNAL EAR CANAL AND TYMPANIC MEMBRANE IN DOGS

Ultrasonographic imaging of the canine external ear canal, tympanic membrane, and tympanic bulla was described in five healthy beagle dogs before and after infusion of saline into the ear canal. Saline served as an acoustic window. With this method, the external ear canal, and tympanic bulla were visible in the same imaging plane and the integrity of the tympanic membrane could be evaluated indirectly by confirming an intact tympanic membrane, which appeared at the end of the ear canal as a hyperechoic line with reverberation. Experimentally, perforated tympanic membrane could be evaluated by identifying anechoic saline in the tympanic bulla lumen. The air and fluid-filled tympanic bulla were also visualized. Ultrasonography with saline as an acoustic window appears to be helpful for the evaluation of the external ear canal, tympanic membrane, and tympanic bulla and it may have the potential to be a useful clinical tool in evaluation of integrity of the tympanic membrane.     

基因型鹅副粘病毒NA-1株全基因组的克隆及特性分析

将本实验室分离保存的NA-1株鹅副粘病毒(GPMV)经SPF鸡胚增殖,收集鸡胚尿囊液进行病毒纯化,提取病毒基因组RNA。参考GenBank已收录的GPMVZJ1株基因组序列,设计了8对特异性引物,RT-PCR法分别特异性的扩增出病毒各基因片段,并将各目的基因片段回收纯化,克隆PGEM-T载体,转化大肠杆菌DH5α,小提质粒选取阳性克隆酶切鉴定并进行序列测定,对测定结果进行拼接得到全长cDNA序列(GenBank登录号:DQ659677)。NA-1株核苷酸比新城疫病毒(NDV)核苷酸多6个碱基,位于1644~1645nt之间,符合NDV核苷酸“六碱基原则”。序列分析结果表明,GPMVNA-1株与各地代表株核苷酸同源性81.2%~91.3%。同时根据各毒株F基因开放阅读框前389个核苷酸序列绘制出系统发育进化树,结果表明GPMNNA-1株与SF02和QY97病毒株同属于基因型,不同于基因IX型NDV的国家标准株F48E9和基因型的LaSota传统弱毒株。     

高邮鸭PRL基因内含子1的SNP检测及其与产蛋性状的相关分析

利用PCR—RFLP技术对高邮鸭催乳素（Prolactin，PRL）基因内含子1进行了SNP检测和测序分析，并对该基因与高邮鸭产蛋性状的相关性进行了研究。结果表明：PRL基因内含子1存在两个Dra Ⅰ酶切位点，其中1个位点具有DraⅠ多态性。该位点由于在1326位点发生了T→C的碱基突变，产生了AA、AB和BB3种基因型。基因型BB和等位基因B的频率最高；BB基因型个体30周龄蛋重极显著高于AB基因型个体（P〈0．01）；AB基因型个体的双黄率显著高于BB基因型个体（P〈0．05）；3种基因型间产蛋数、最长连产天数和开产体重无显著差异。     

四倍体高粱与约翰逊草杂交种饲用品质分析

利用四倍体高粱品系"四沈甜"、不育系"四6A"和约翰逊草,配置"四沈甜"×约翰逊草、"四6A"×约翰逊草和"四6A"×"四沈甜"杂交种,分析了杂交种及杂种分离世代的不同生长阶段的杂种优势及饲草品质。结果表明,在孕穗期各供试材料粗蛋白质含量最高,杂交种蛋白质含量达到10%～15%,随着发育期的推进,含量有所下降。抽穗期,粗脂肪含量达到6.1%～7.9%。各材料的粗纤维含量都在30%以上,不同生长阶段差异不显著。单位能量值在抽穗期最高,达到18MJ/kg左右。综合各营养成分含量和单位能量分析,初步认为四倍体高粱与约翰逊草杂交种可以作为饲草应用,抽穗期为刈割和利用的最佳时期。     

黄土高原不同林龄土壤质地和矿质元素差异研究

对黄土高原不同林龄和不同树种条件下土壤的机械组成和矿质元素含量进行了分析研究,结果表明:①成林土壤砂粒、粉粒含量低于中幼林土壤,粘粒含量高于中幼林土壤,且呈显著性差异;②成林土壤Al2O3、Fe2O3、TiO2、MnO2、K2O含量相对于中幼林土壤有不同程度的增加,除MnO2外,差异均为极显著;成林土壤中Al2O3、Fe2O3、TiO2、MnO2在土壤中下层有不同程度的富集,SiO2、CaO含量降低;③矿质元素含量与土壤质地密切相关。