Detect unique molecular structure associated with physiochemical properties in CDC varieties of oat grain with unique nutrient traits [Feed Type vs. Milling Type] in comparison with barley grain using advanced molecular spectroscopy as a non-destructive biological tool

The objective of this study was to determinate grain unique protein inherent molecular structure that are related physiochemical and nutrient profiles in CDC developed oat varieties [CDC Nasser (Feed Type) and CDC Seabiscuit (Milling Type)] grown in cool climate condition in western Canada in comparison with conventional barley variety of CDC Meredith as a control using advanced molecular spectroscopy. Multivariate analyses, including an agglomerative hierarchical cluster analysis (CLA) and principal component analysis (PCA), were performed to identify protein molecular structural differences among the grains. The results revealed that CDC Seabiscuit contained greater (P < 0.05) protein structural Amide I and II than CDC Nasser and CDC Meredith, while the greater (P < 0.005) structural Amide I to II area and height ratios was detected in CDC Meredith. New oat grains had greater (P < 0.05) β-sheet height than barley grains, however, there was no difference in α-helix to β-sheet ratio values among the varieties. In conclusion, CDC Nasser and CDC Meredith had no difference in protein molecular structural features, while CDC Seabiscuit contains different protein structural characteristics as compared to CDC Meredith grain. The molecular structure features are highly associated with physiochemical and nutrient profiles in grains, which indicate that it also affect nutrient utilization and availability.     

Identification of an H1N1 subtype of swine influenza virus and serological analysis

To investigate the epizootic of swine influenza virus(SIV), 60 nasal swabs were collected from a clinical cases of pig farm in Tai'an City, Shandong Province of China in April 2017. SIV was isolated by inoculating into 10-day-old Special Pathogen Free embryonated eggs and the whole genome was sequenced. An H1N1 subtype SIV was isolated and designated as A/swine/Shandong/TA04/2017(H1N1). Phylogenetic analysis showed that apart from the polymerase A(PA) fragment belonging to the 2009 pandemic H1N1 branch, seven genome segments belonged to avian-like H1N1 influenza virus lineage. The cleavage site sequence of the hemagglutinin(HA) protein was PSIQSR↓G, which is a typical molecular biological characteristic. Five potential N-glycosylation sites(N14, N26, N277, N484 and N543) were found in the HA gene. To further investigate the epidemiology of SIV in this farm, the 995 serum samples were assessed with EAH1N1 2009 pandemic H1N1 and H3 N2 antigens. The results showed that the total positive rate was 65.43%. The positive rates of single virus infection detected by EAH1N1, 2009 pdmH1N1 and H3 N2 for serum HI(Hemagglutination inhibition) were 48.35, 30.85 and 7.47%, respectively. The results showed that SIV in Shandong Province has been reassorted in some segments and the SIV-positive rate was high on the SIV outbreak farm. These data provide evidence of an epizootic of SIV.     

小叶杨基因资源异地保存及苗期生物学特性分析

分别从东北、西北和华北收集小叶杨各类资源。并建立了异地保存基因库。同时，对小叶杨资源苗期的生长、物候期、形态特征以及同工酶谱进行了观测和分析。     

新疆农田土壤有机质含量及组成特征

目前新疆农田土壤有机质含量在5．30～24．21g／kg之间，平均14．65g／kg，与第二次土壤普查相比，新疆农田土壤有机质含量呈上升趋势；胡敏素碳含量占到有机质碳量的53％～70％，平均61％，腐殖酸碳量只占39％。     

二丁酰环腺苷酸对猪胴体组成和肉品质的影响

通过皮下注射和饲料添加不同剂量dbcAMP进行饲养试验 ,研究其对肥育猪胴体组成和肉品质的影响。结果表明 :皮下注射 1.0mg/kg和饲料添加 2 0mg/kg对改善肥育猪的胴体组成和肉品质效果最好 ,眼肌面积分别提高31.99%和 36 .39% (P <0 .0 5 ) ;瘦肉率分别提高 12 .4 2 %和 11.94 % (P <0 .0 5 ) ;背膘厚分别降低 19.95 %和 2 0 .97%(P <0 .0 5 )。肌肉粗蛋白含量分别提高 10 .87%和 16 .5 4 % (P <0 .0 5 )。     

封闭型植物工厂发展现状与展望

经过多年探索和发展,封闭型植物工厂无论在材料、装置和技术都取得了快速进步,生产产量成数倍增长。在介绍发展封闭型植物工厂必要性的基础上,着重分析了封闭型植物工厂的发展现状、设施分类、栽培形式选择、栽培技术选择,最后指出了存在的问题,并对未来的封闭型植物工厂外部设施及其栽培技术进行了展望。     

甜玉米‘闽甜208’的生育特性与群体结构分析

多年生育特性观测表明,‘闽甜208’甜玉米春播采青生育期86-90d,秋播采青生育期73-76d,生育动态呈现苗期弱,中间旺长稳定期短,后期早衰快的生育特性,栽培上要注重精细播种,各项田间管理要及时到位。群体结构试验表明,‘闽甜208’叶面积系数（LAI）及叶片净同率（NAR）较高,峰值持续时间较长,大喇叭口至抽雄期LAI对NAR影响最大,通过密植保证一定的总粒数,增加抽雄后群体叶面积及干物质积累,提高成粒率,是高产的关键措施,适宜种植密度为4.95万-5.40万株·hm-2。     

东京野茉莉花色成分的初步研究

对东京野茉莉花色成分进行了初步的探究。结果表明:通过特征颜色反应和光谱扫描说明了其花瓣细胞内有异黄酮、儿茶精类,还有叶绿素a、b,可能含有胡萝卜素、黄酮类化合物,以及无色花色素,可能不含查耳酮或橙酮。     

黄连及黄连须、黄连茎叶体外抗氧化活性的谱效关系研究

测定黄连与黄连须、黄连茎叶主要生物碱含量并建立高效液相(HPLC)指纹图谱,分析其抗氧化活性的谱效关系。采用HPLC分析比较黄连及黄连须、黄连茎叶中小檗碱、表小檗碱、药根碱、黄连碱、巴马汀含量差异并建立指纹图谱,通过羟自由基(·OH)、1,1-二苯基-2-三硝基苯肼(DPPH)自由基、2,2-联氮基双(3-乙基苯并噻唑啉-6-磺酸)(ABTS)自由基体外清除试验与总还原力试验评价其体外抗氧化活性差异,应用Pearson相关系数法分析HPLC指纹图谱与抗氧化活性的谱效关系。结果发现小檗碱、巴马汀含量为黄连>黄连茎叶>黄连须;药根碱、黄连碱和表小檗碱含量为黄连>黄连须>黄连茎叶;总还原力为黄连>黄连茎叶>黄连须,·OH、DPPH、ABTS·+自由基清除力为黄连茎叶>黄连>黄连须;总还原力与各峰均有较强相关性,·OH、DPPH、ABTS·+自由基清除力与4号峰相关性较强。说明黄连茎叶体外抗氧化活性较强,黄连须与黄连茎叶均有一定药用开发价值。