Identification of an H1N1 subtype of swine influenza virus and serological analysis |
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Authors: | Fa-chao SUN Min TAN Yuan-chao ZHANG Yu-chao WANG Sheng-liang CAO Guo-fei DING Fang-yuan CONG Li-hong GUO Si-dang LIU Yi-hong XIAO |
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Institution: | Department of Basic Veterinary Medicine, College of Animal Science and Veterinary Medicine/Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention/Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Shandong Agricultural University, Tai''an 271018, P.R.China |
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Abstract: | To investigate the epizootic of swine influenza virus (SIV), 60 nasal swabs were collected from a clinical cases of pig farm in Tai'an City, Shandong Province of China in April 2017. SIV was isolated by inoculating into 10-day-old Special Pathogen Free embryonated eggs and the whole genome was sequenced. An H1N1 subtype SIV was isolated and designated as A/swine/Shandong/TA04/2017(H1N1). Phylogenetic analysis showed that apart from the polymerase A (PA) fragment belonging to the 2009 pandemic H1N1 branch, seven genome segments belonged to avian-like H1N1 influenza virus lineage. The cleavage site sequence of the hemagglutinin (HA) protein was PSIQSR↓G, which is a typical molecular biological characteristic. Five potential N-glycosylation sites (N14, N26, N277, N484 and N543) were found in the HA gene. To further investigate the epidemiology of SIV in this farm, the 995 serum samples were assessed with EAH1N1 2009 pandemic H1N1 and H3N2 antigens. The results showed that the total positive rate was 65.43%. The positive rates of single virus infection detected by EAH1N1, 2009pdmH1N1 and H3N2 for serum HI (Hemagglutination inhibition) were 48.35, 30.85 and 7.47%, respectively. The results showed that SIV in Shandong Province has been reassorted in some segments and the SIV-positive rate was high on the SIV outbreak farm. These data provide evidence of an epizootic of SIV. |
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Keywords: | Correspondence XIAO Yi-hong Tel:+86-538-8242478 SIV H1N1 subtype genetic analysis molecular feature serology |
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