仔猪PRRS母源抗体消长规律的测定

##正##为消除或降低母源抗体对仔猪猪繁殖与呼吸障碍综合征疫苗免疫的抑制,同时防止猪繁殖与呼吸障碍综合征病毒在免疫空白期对仔猪的侵袭,试验通过酶联免疫吸附试验(ELISA)分别对产前1个月免疫猪繁殖与呼吸系统综合征灭     

一例猪繁殖与呼吸综合征高致病性毒株与链球菌混合感染的实验室诊断

某规模化自繁自养猪场产房母猪出现流产,保育猪关节肿胀、倒地不起、大量死亡,为研究发病原因,采集流产胎儿、死亡仔猪的肺脏、脾脏、淋巴结和关节液,进行分子生物学检测,病毒和细菌分离鉴定,并检测不同胎龄母猪猪繁殖与呼吸综合征病毒抗体,结果显示猪繁殖与呼吸综合征病毒检测为阳性,使用PAM分离到一株猪繁殖与呼吸综合征病毒,经序列鉴定为高致病性毒株;抗体结果表明四胎及以上母猪猪繁殖与呼吸综合征病毒抗体阳性率异常升高;病料分离的细菌经鉴定为猪链球菌,经实验室诊断确定猪场发病的原因为猪繁殖与呼吸综合征高致病性毒株与猪链球菌混合感染。     

猪繁殖与呼吸综合征灭活疫苗的研制

选用在美国成功使用的PRRSV疫苗株 ,在Marc -14 5传代细胞上增殖出较高滴度的PRRSV。该病毒经甲醛灭活后 ,与一定量的油佐剂乳化制成安全性好、保护率高的PRRS油佐剂灭活疫苗。经实验室检验和田间试验 ,证明该疫苗安全可靠、免疫效果好 ,适于在国内推广使用     

猪繁殖与呼吸综合征重组核衣壳蛋白(N)-ELISA诊断方法的建立与应用

将猪繁殖与呼吸综合征病毒国内分离株ＣＨ－ｌａ的核衣壳蛋白基因定向克隆到ｐＢｌｕｅＢａｃＨｉｓＢ转移载体ＰＨ启动子下游,与苜蓿银蚊夜蛾多核型多角体病毒线性化ＤＮＡ共转染Ｓｆ９细胞。获得能隐定表达核衣壳蛋白（Ｎ 蛋白）的重组杆状病毒。利用昆虫杆状病毒表达系统表达的猪繁殖与呼吸综合征病毒重组Ｎ蛋白作为抗原,包被聚苯乙烯微量反应板,建立了猪繁殖与呼吸综合征重组Ｎ蛋白－ＥＬＩＳＡ诊断方法。试验证明,该方法对其他８种猪疫病阳性血清均无交叉反应,对标准阳性样品的检出率为１００％,具有敏感性高、特异性强的特点。     

猪繁殖与呼吸综合征病毒HuB-20株特异性抗体IgG的制备

应用猪繁殖与呼吸综合征病毒HuB-20株活疫苗免疫兔,制备兔抗猪繁殖与呼吸综合征病毒毒株的特异性抗体IgG,应用15%SDS-PAGE和Western-blot进行纯化鉴定.结果表明:抗猪繁殖与呼吸综合征病毒HuB-20株特异性抗体IgG经15%SDS-PAGE纯化分析和Western-blot的鉴定显示该特异性抗体IgG具有很好的免疫原性,特异性强,仅与该病毒毒株的ORF5发生特异性结合.说明兔抗猪繁殖与呼吸综合征病毒HuB-20株特异性抗体具有良好的免疫原性和特异性,可与猪繁殖与呼吸综合征病毒ORF5特异性结合,可以作为诊断试剂和诊断试剂盒的一抗.     

猪繁殖—呼吸综合征和猪瘟混合感染的诊断与防制

以耳部发绀为特征的病猪，经流行病学调查，临床观察、病理剖检、猪瘟荧光抗栓检验和猪瘟兔体交互免疫试验、猪繁殖－呼吸综合征病毒抗体检测等检验，诊断为猪繁殖－呼吸综合征和猪瘟混合染，采取加强猪瘟免疫等综合措施，疫情得到控制。     

PRRSV缺失变异毒株JL/07/SW株的分离鉴定及序列分析

分离并鉴定了1株猪繁殖与呼吸综合征病毒,经病毒生物学特性测定、血清学试验、病毒基因鉴定,确定为美洲型猪繁殖与呼吸综合征病毒,将其命名为JL/07/SW株.根据猪繁殖与呼吸综合征病毒VR-2332株及变异毒株的核苷酸序列,设计合成了针对NSP2变异序列、N基因以及GP5基因的引物,扩增出正确的序列,经测定的序列比对后,发现该毒株为美洲型变异毒株,NSP2上缺失了30个氨基酸,而GP5和M基因相对保守.     

一起猪高致病性蓝耳病的病例报告

<正>猪高致病性蓝耳病又称猪繁殖与呼吸综合征(PRRS),是以母猪流产及仔猪呼吸系统障碍为主要病征的传染病,病原为猪繁殖与呼吸综合征病毒(PRRSV)。该病毒传染性强,而且感染机体后致免疫抑制及各种并发症,给养猪业造成巨大危害和经济损失。本研究通过山东某猪场送检病料,通过临床症状、剖检变化及实验室诊断,经PCR病原学鉴定确定是猪蓝耳病。猪繁殖与呼吸综合征(PRRS),是由猪繁殖与呼吸综合征病毒(PRRSV)引起的,主要以引起母猪发热、厌食和     

某猪场爆发高致病性猪繁殖与呼吸综合征的诊断

2007年6月份,某猪场爆发一起以高热、高发病率和高死亡率为特征的传染性疾病,临诊症状主要表现为高烧、皮肤发红、怀孕母猪流产,剖检主要特征是间质性肺炎。经过病原分离,获得一株能够引起Marc-145细胞发生病变的病毒。进一步通过血清中和试验、血凝试验、RT-PCR,确定此次分离的病毒为高致病性猪繁殖与呼吸综合征病毒。综合该病的流行病学特征、临床症状、剖检病理变化以及实验室检验,确诊该病为高致病性猪繁殖与呼吸综合征病毒引起的猪繁殖与呼吸综合征。     

高致病性猪蓝耳病的临床诊断与防治

高致病性猪蓝耳病是由猪繁殖与呼吸综合征病毒变异株(NVDC-JXA1株)引发的一种以体温高热不退、皮肤发红、呼吸困难、死亡率高为特征的烈性传染病。近年来,猪繁殖与呼吸综合征病毒的变异现象引起了人们的关注,引发高致病性猪蓝耳病的NVDC-JXA1株就是猪繁殖与呼吸综合征病毒的     

高致病性猪蓝耳病弱毒疫苗与灭活疫苗的免疫效果评估试验

在相同饲养条件下,对35日龄仔猪分别使用高致病性猪蓝耳病弱毒疫苗与灭活疫苗进行免疫接种。在接种当天、接种后28d、58d分别检测高致病性猪蓝耳病免疫抗体水平。结果显示,使用弱毒疫苗免疫的试验猪,其高致病性猪蓝耳病抗体上升速度和抗体水平均优于使用灭活疫苗的试验猪。弱毒疫苗可在接种后21～28d产生免疫力,免疫期为4个月。猪繁殖与呼吸综合征流行地区应该首先考虑使用弱毒疫苗。     

猪蓝耳病卵黄抗体的制备与初步应用试验

为了制备猪蓝耳病(PRRS)高免卵黄抗体,并对其治疗效果进行研究,采用高致病性猪蓝耳病灭活疫苗(NVDC-JXA1株)免疫产蛋鸡,收集高免蛋,通过水稀释提取法制备PRRS高免卵黄抗体,对其进行无菌检验、安全性检测、效力检验及动物保护性试验,并将其应用于临床实践,观察其治疗效果。结果发现,该方法制备的PRRS高免卵黄抗体琼脂扩散效价达到1:32,保护率达100%,临床试验治愈率为84.5%。研究表明,PRRS特异性卵黄抗体的制备具有操作简便、成本低廉等特点,可以用于PRRS临床治疗。     

Comparative evaluation of PRRS virus infection in vaccinated and naïve pigs

The purpose of this study was to evaluate the time-course of the immune response to a field Porcine Respiratory and Reproductive Syndrome virus (PRRSV) strain in PRRS-naïve, untreated pigs, as well as in four groups of age and breed-matched pigs injected with a live attenuated PRRS vaccine, its adjuvant, an inactivated PRRS vaccine and an irrelevant, inactivated Porcine Circovirus type 2 (PCV2) vaccine, respectively. PRRSV infection was confirmed in all groups by PCR and antibody assays. The antibody response measured by ELISA took place earlier in pigs injected with the live attenuated vaccine, which also developed a much stronger serum-neutralizing antibody response to the vaccine strain. Yet, no clear protection was evidenced in terms of viremia against the field virus strain, which showed 11.1% nucleotide divergence in ORF7 from the vaccine strain. In vitro, the interferon (IFN)-γ response to PRRSV was almost absent on PVD 60 in all groups under study, whereas the prevalence of interleukin (IL)-10 responses to PRRSV was fairly high in PCV2-vaccinated animals, only. Results indicate that distinct patterns of immune response to a field PRRSV strain can be recognized in PRRS-vaccinated and naïve pigs, which probably underlies fundamental differences in the development and differentiation of PRRSV-specific immune effector cells.     

不同类型猪蓝耳病疫苗的体液免疫效果比较

为科学评价不同猪蓝耳病疫苗对生产母猪和仔猪免疫效果,选取11个规模猪场,分别使用猪蓝耳病进口弱毒疫苗、国产弱毒疫苗、普通灭活疫苗、猪高致病性蓝耳病灭活疫苗对生产母猪和仔猪进行免疫,二免28d后,采集免疫猪群的血清,进行免疫抗体检测,结果表明:生产母猪使用不同类型的蓝耳病疫苗免疫后所产生的抗体阳性率均高于仔猪免疫后所产生的抗体阳性率。     

猪繁殖与呼吸综合征免疫防制新途径的研究进展

猪繁殖与呼吸综合征(porcine reproductive and respiratory syndrome,PRRS)是一种主要表现为母猪繁殖障碍与仔猪呼吸道症状的传染病。近年来,猪繁殖与呼吸综合征病毒(porcine reproductive and respiratory syndrome virus,PRRSV)变异株不断出现,免疫逃避及持续性感染使得猪群发病率或复发率均相继增高,给养猪业带来了巨大的损失。目前所采用的胃肠道途径接种活疫苗或灭活疫苗的方法无法诱导对猪群的全面保护作用。为减少养猪业的经济损失,亟需研制新防制方法和新疫苗接种途径。作者主要从黏膜免疫的免疫部位、呼吸道保护性黏膜免疫反应诱导、黏膜免疫途径、佐剂的选择及病毒的免疫抑制反应等方面简要论述了有效防制PRRSV的黏膜免疫方法的研究进展,为进一步了解黏膜免疫抵御PRRSV突变株感染及黏膜疫苗研制等方面提供有用的信息。     

猪口蹄疫、猪瘟、猪蓝耳病疫苗最佳免疫方法研究

为探索生猪重大疫病的科学免疫方法,确定最佳免疫程序,笔者设计并开展了口蹄疫（FMD）合成肽苗（或灭活苗）、猪瘟（HC）脾淋苗、高致病性蓝耳病弱毒疫苗（或灭活苗）的不同组合注射、单独注射、不同疫苗类型注射的试验研究.结果表明：3种疫苗不同组合注射或单独注射,以及使用不同类型疫苗注射,其免疫副反应均无明显差异;平均免疫抗体效价及群体合格率各组间存有差异;采用猪口蹄疫、猪瘟、猪蓝耳病3种疫苗同时分点注射,以及仔猪首免口蹄疫、猪蓝耳苗,再免疫口蹄疫、猪瘟苗的免疫效果最佳.     

猪瘟苗与猪蓝耳苗混合免疫的效果报告

本试验主要研究猪瘟、猪蓝耳病弱毒疫苗等量混合注射后再注射猪口蹄疫苗的免疫效果,结果表明,用这种方式免疫后,猪瘟、猪蓝耳病的抗体合格率均超过70%的标准,适用于春秋季集中免疫,而口蹄疫抗体效价则因疫苗不同而差异较大,合成肽苗明显好于O型灭活苗。     

Preparation and evaluation of inactivated avian Metapneumovirus vaccine from recently isolated Egyptian strain

Inactivated avian Metapneumovirus (aMPV) or turkey rhinotracheitis (TRT) virus vaccine was prepared from an Egyptian strain (Giza TRT-4). The virus was propagated in Vero cells and inactivated by binary ethyleneimine. The inactivated virus solution was tested for its sterility, purity, and safety. Then, it was mixed withNigella sativa oil as nonspecific immune-stimulant adjuvant. Physical characterization of oil prepared vaccine like viscosity and emulsion stability was investigated. An experiment was designed to evaluate the locally prepared aMPV vaccine in a comparison to commercial vaccines either inactivated or live attenuated. The obtained results showed that the locally prepared aMPV vaccine gave significantly higher humoral immune response when measured by ELISA and significantly higher cell mediated immunity by evaluating phagocytic activity of inoculated turkey poults with higher protection rate reached up to 100% after challenge with wild-type virus.     

Shedding of porcine reproductive and respiratory syndrome virus in mammary gland secretions of sows.

OBJECTIVE: To document shedding of porcine reproductive and respiratory syndrome (PRRS) virus in mammary gland secretions of experimentally inoculated sows, to evaluate effects of vaccination during gestation on virus shedding during the subsequent lactation, and to evaluate shedding of PRRS virus in milk of sows in commercial herds. ANIMALS: 6 sows seronegative for PRRS virus were used for experiment 1, and 2 sows were retained for experiment 2. For experiment 3, 202 sows in commercial herds were used. PROCEDURE: In experiment 1, 2 sows were inoculated with PRRS virus, 2 sows were vaccinated with modified-live PRRS virus vaccine, and 2 sows served as control pigs. Mammary gland secretions were assayed for PRRS virus. In experiment 2, pregnant vaccinated sows from experiment 1 were vaccinated with another modified-live PRRS virus vaccine. Mammary gland secretions were assayed in the same manner as for experiment 1. For experiment 3, milk collected from 202 sows in commercial herds was assayed for PRRS virus. RESULTS: In experiment 1, PRRS virus was detected in mammary gland secretions of both vaccinated and 1 of 2 virus-inoculated sows. In experiment 2, virus was not detected in samples from either vaccinated sow. In experiment 3, all samples yielded negative results. CONCLUSIONS AND CLINICAL RELEVANCE: Na?ve sows inoculated late in gestation shed PRRS virus in mammary secretions. Previous vaccination appeared to prevent shedding during the subsequent lactation. Results for samples obtained from sows in commercial herds suggested that virus shedding in mammary gland secretions of such sows is uncommon.     

板蓝根多糖对PRRSV疫苗的免疫增强试验

选取健康长白二元杂交断奶仔猪18只，随机分为3组，即对照组、试验Ⅰ组、试验Ⅱ组。试验Ⅰ组注射猪繁殖障碍与呼吸道综合征疫苗，试验Ⅱ组注射猪繁殖障碍与呼吸道综合征疫苗之外，再注射板兰根多糖。再对试验猪进行猪繁殖与呼吸综合征病毒接种2．0mL／头。结果表明板蓝根多糖和猪繁殖障碍与呼吸道综合征疫苗联合使用，能提高PRRSV保护水平，两者联合应用，明显降低猪繁殖障碍与呼吸道综合征的发病率和死亡率。