规模猪场猪伪狂犬病的诊断实例

天津某规模猪场发生妊娠母猪流产、产死胎及部分仔猪发病的情况,为确诊病原,分别对流产胎儿的组织和母猪血清进行了实验室检测。流产胎儿的组织经PCR或RT-PCR检测,猪伪狂犬病毒(PRV)为阳性,猪圆环病毒(PCV)、猪瘟病毒(CSFV)和猪繁殖与呼吸综合征病毒(PRRSV)为阴性。经猪伪狂犬病毒野毒株实时荧光PCR检测胎儿组织为阳性,检测母猪血清中猪伪狂犬gE抗体为阳性;用病毒的分离培养试验分离到了猪伪狂犬病毒,根据临床症状、抗原抗体检测及病毒的分离培养试验确诊该猪场存在猪伪狂犬病毒野毒感染。     

1株临床高致病性猪繁殖与呼吸综合征病毒的分离与鉴定

本研究从江苏省某猪场患有明显呼吸道临床症状和高热死亡的仔猪肺脏组织中分离到一株病毒,经病毒生物学特性测定、血清学试验、病毒基因鉴定,确定为美洲型猪繁殖与呼吸综合征病毒(PRRSV)。人工猪体感染发病试验表明,该分离株可以引起商品仔猪出现明显临床症状和死亡,证实我国已经出现临床高致病性PRRSV毒株。     

贵州省某规模化猪场暴发猪瘟与链球菌病混合感染的诊断

为了解贵州某规模化猪场发病断奶仔猪死亡原因,本研究采用流行病学调查、临床症状观察、病理解剖诊断和RT-PCR检测等方法,对该规模化猪场发病猪进行了诊断。试验结果表明,通过流行病学调查、剖检病理和猪瘟抗体快速金标检测卡检测,初步诊断该猪场发病猪疑似猪瘟病毒和细菌混合感染;RT-PCR检测核酸确诊发病猪为猪瘟病毒感染;细菌培养分离、生化特性鉴定和动物致病性试验确诊为猪链球菌。造成该猪场断奶仔猪发病死亡的原因为猪瘟病毒和猪链球菌混合感染所致。     

高致病性猪蓝耳病的实验室诊断

采用猪繁殖与呼吸综合征病毒NSP2基因RT-PCR检测方法和猪繁殖与呼吸综合征病毒变异毒株试剂盒检测方法,对六盘水市钟山区某猪场发生的一起疑似高致病性猪蓝耳病进行实验室诊断,确诊为由猪繁殖与呼吸综合征病毒变异毒株引起的高致病性猪蓝耳病。     

高致病性猪蓝耳病的诊断及综合防制

高致病性猪蓝耳病（PRRS）是由猪繁殖与呼吸障碍综合征病毒变异毒株（PRRSV）引起的一种急性高致病性传染病。主要表现为母猪呼吸困难和流产、产死胎、木乃伊胎、弱仔,流产率可达30％以上等症状;仔猪发病率可达100％,死亡率可达50％以上;育肥猪也可发病死亡。     

一例PRRS和传染性胸膜肺炎混合感染的诊断

为了解云南省宣威市某规模化猪场育肥猪发生大量死亡的原因,试验采用临床症状观察、剖检病变、细菌分离培养、ELISA和PCR/RT-PCR方法进行检测,并对所分离的细菌进行形态观察、生化鉴定和药敏试验。结果表明:所分离的细菌形态特征及生化特性与猪胸膜肺炎放线杆菌一致,分离菌对氟苯尼考和卡那霉素高度敏感。RT-PCR法检出猪繁殖与呼吸综合征病毒特异性条带,判为阳性;样品的猪繁殖与呼吸综合征病毒抗体阳性率为61%(11/18)。PCR法检出胸膜肺炎放线杆菌,抗体阳性率为78%(14/18)。说明该猪场猪大量死亡的原因为猪繁殖与呼吸综合征和传染性胸膜肺炎混合感染。     

猪圆环病毒2型、猪繁殖与呼吸综合征病毒、链球菌混合感染的实验室诊断

为确诊贵州省某猪场发病原因,对送检病料进行细菌分离鉴定和病毒核酸检测。结果:猪繁殖与呼吸综合征病毒实时定量荧光RT-PCR核酸检测为阳性;猪圆环病毒2型PCR扩增出大小为325 bp目的基因条带;细菌分离与生化鉴定显示存在链球菌感染,且药敏试验结果表明分离菌对硫酸安普霉素、延胡索酸泰妙菌素高度敏感。实验结果诊断为猪圆环病毒2型、猪繁殖与呼吸综合征病毒、链球菌混合感染。     

PRV、PRRSV和链球菌混合感染的诊断

为了解贵州某猪场猪只死亡原因,采用流行病学调查、临床症状观察、病理剖检诊断、细菌分离鉴定、生化鉴定和PCR/RT-PCR检测诊断等方法对发病死亡猪只进行诊断。结果表明:根据流行病学调查、病理剖检初步诊断送检病猪疑似猪瘟(CSF)、猪圆环病毒2型(PCV-2)、猪繁殖与呼吸综合征(PRRS)、猪细小病毒病(PP)、猪伪狂犬病(PR)、猪流感病毒(SI)和细菌感染,经病毒核酸检测,结果猪伪狂犬病病毒(PRV)PCR检测和猪繁殖与呼吸综合征病毒(PRRSV)RT-PCR检测均检出特异性条带,判断为阳性;细菌分离培养、生化鉴定确诊为链球菌感染。说明造成该猪场猪只发病死亡的原因为PRRSV、PRV和链球菌混合感染。     

一起猪高致病性蓝耳病的病例报告

<正>猪高致病性蓝耳病又称猪繁殖与呼吸综合征(PRRS),是以母猪流产及仔猪呼吸系统障碍为主要病征的传染病,病原为猪繁殖与呼吸综合征病毒(PRRSV)。该病毒传染性强,而且感染机体后致免疫抑制及各种并发症,给养猪业造成巨大危害和经济损失。本研究通过山东某猪场送检病料,通过临床症状、剖检变化及实验室诊断,经PCR病原学鉴定确定是猪蓝耳病。猪繁殖与呼吸综合征(PRRS),是由猪繁殖与呼吸综合征病毒(PRRSV)引起的,主要以引起母猪发热、厌食和     

猪繁殖与呼吸综合征与猪圆环病毒2型混合感染的病因分析及综合防控

甘肃省某规模猪场,500头不同胎次母猪,日流产2～4头,主要表现为产死胎、木乃伊胎,平均死亡淘汰率高达20.05%。为确定流产原因,分别采集7份流产胎儿组织样品,进行常见母猪流产疫病的病原学检测;同时随机抽取2份饲料样品进行黄曲霉毒素检测。检测结果表明:猪繁殖与呼吸综合征病毒、猪圆环病毒2型核酸检测均为阳性,猪瘟病毒核酸检测为阴性;饲料中黄曲霉毒素未超标。结果显示,猪繁殖与呼吸综合征病毒与猪圆环病毒2型的混合感染是导致该规模场母猪流产的主要原因。     

某规模化猪场伪狂犬病的诊断

河南平顶山某猪场母猪出现较严重的流产和产死胎现象,且50日龄~70日龄仔猪出现神经症状,根据临床表现初步诊断为伪狂犬病。为排除猪繁殖与呼吸综合征和猪瘟,进行了实验室诊断。应用ELISA方法检测发病保育猪及母猪血清的伪狂犬病病毒野毒株gE抗体,并对发病仔猪病料进行了伪狂犬病病毒(PRV)、猪繁殖与呼吸综合征病毒(PRRSV)和猪瘟病毒(CSFV)的实时荧光定量PCR检测。结果显示,伪狂犬病病毒野毒抗体阳性,实时荧光定量PCR检测确定仔猪病料中PRV核酸阳性,PRRSV和CSFV核酸阴性。结合临床症状及实验室检测,确诊该猪场发生的是猪伪狂犬病。     

Sow performance in an endemically porcine reproductive and respiratory syndrome (PRRS)-infected farm after sow vaccination with an attenuated PRRS vaccine

The objective of this field study was to evaluate in an endemically porcine reproductive and respiratory syndrome (PRRS) virus-infected farm the reproductive performance of sows after their vaccination with a PRRS attenuated vaccine. In a farrow-to-finish pig farm with history of endemic PRRS virus infection, a total of 200 gilts and sows were used. They were divided in 2 groups of 100 animals. The first group was used as untreated controls, while the animals of the second group were vaccinated against PRRS virus using the attenuated Porcilis PRRS vaccine (Intervet International, The Netherlands) based on European strain. All health and reproductive parameters were recorded from the time of vaccination up to next weaning. No adverse systemic or local reactions or side effects relative to vaccination were noted. Compared to controls, vaccinated sows showed significantly improved farrowing rate (89% versus 78%) and a tendency for fewer returns to oestrus, particularly those at irregular intervals. Fewer sows farrowed prematurely and showed post-partum dysgalactia syndrome, but more live pigs were born and weaned in each litter after vaccination. It was concluded that vaccination of sows with Porcilis PRRS attenuated vaccine in farms with endemic PRRSV infection has beneficial effects on their health and fertility.     

猪繁殖与呼吸综合征的研究进展及防控措施

猪繁殖与呼吸综合征（Porcine reproductive and respiratory syndrome,PRRS）是由猪繁殖与呼吸综合征病毒（Porcine reproductive and respiratory syndrome virus,PRRSV）感染引起的一种以妊娠母猪发热流产、仔猪高死亡率和各年龄段猪呼吸功能障碍为主要特征的高度接触性传染病。目前,该病在全球范围内均有分布,对养猪业造成的经济损失不计其数。自1996年我国首次分离PRRSV到如今,该病已在我国横行了25年之久,却依旧无法有效防治。文章对目前国内外猪繁殖与呼吸综合征病原、流行病学、临床症状以及疫苗免疫和综合防控措施等方面的研究进展进行综述,以期为该病的防控提供科学依据。     

Experimental inoculation of late term pregnant sows with a field isolate of porcine reproductive and respiratory syndrome vaccine-derived virus.

The use of a live attenuated porcine reproductive and respiratory syndrome virus (PRRSV) vaccine in piglets has been associated with reproductive disorders in non-vaccinated sows. Vaccine-derived virus (VDV) has been isolated from foetuses, stillborn pigs, and dead piglets, indicating that the live vaccine spread from vaccinated piglets to non-vaccinated sows, and that the virus might be implicated in the severe reproductive problems observed. In the present study, one such VDV isolate was used to experimentally infect pregnant sows in the last trimester. The chosen isolate, which had more than 99.6% identity to the attenuated vaccine virus, originated from the lungs of a stillborn pig from a swine herd with a sudden high level of stillborn pigs and increased piglet mortality in the nursing period. Intranasal inoculation of sows with the virus isolate resulted in congenital infection, foetal death, and preweaning pig mortality. As such, the present study showed that vaccine-derived PRRSV can cause disease in swine consistent with PRRS.     

猪繁殖与呼吸综合征病毒、圆环病毒2型、奇异变形杆菌混合感染病例的病原学诊断

为确诊贵州省都匀市某养殖场的猪死亡病因,采集病死猪病料进行猪繁殖与呼吸综合征病毒、猪瘟病毒、猪伪狂犬病病毒、猪圆环病毒2型核酸检测,细菌分离鉴定及药物敏感试验。结果:猪繁殖与呼吸综合征病毒实时荧光定量RT-PCR检测和猪圆环病毒2型PCR检测核酸均为阳性,其余病毒核酸检测均为阴性。细菌分离培养表明存在细菌感染,分子生物学鉴定为奇异变形杆菌;分离菌对硫酸安普霉素、硫酸粘菌素、盐酸大观霉素+盐酸林可霉素、氟苯尼考较敏感,对多种药物产生耐药。结论:确诊病例为猪繁殖与呼吸综合征病毒、猪圆环病毒2型、奇异变形杆菌混合感染。     

贵阳市规模化猪场猪繁殖与呼吸综合征血清学调查研究

试验应用ELISA检测方法对贵阳市3个未免疫猪繁殖与呼吸综合征（PRRS）猪场和9个免疫猪场的后备母猪、生产母猪和5～10周龄断奶仔猪3个猪群进行猪繁殖与呼吸综合征病毒（PRRSV）抗体检测。结果表明：贵阳市3个未免疫猪场中2个有PRRSV感染,猪群抗体阳性率在0％-10％之间,平均阳性率为5％;9个免疫猪场猪群PRRSV抗体阳性率在65％～90％之间,平均阳性率为82．80％,未免疫猪场与免疫猪场猪群抗体平均阳性率相比,差异极显著（P〈0．01）。     

Correlation of cell-mediated immunity against porcine reproductive and respiratory syndrome virus with protection against reproductive failure in sows during outbreaks of porcine reproductive and respiratory syndrome in commercial herds

OBJECTIVE: To determine whether cell-mediated immunity against porcine reproductive and respiratory syndrome (PRRS) virus is correlated with protection against reproductive failure in sows during clinical outbreaks of PRRS in commercial herds. DESIGN: Outbreak investigation in 4 swine breeding herds. ANIMALS: 97 sows. PROCEDURES: On each farm, blood samples were collected from sows with clinical signs (abortion or increased fetal death; case sows) and from clinically normal sows (control sows). The intensity of the cell-mediated immune (CMI) response was determined by use of an interferon-gamma enzyme-linked immunospot (ELISPOT) assay. Multiple logistic regression analyses and t tests were used to compare ELISPOT assay values between case and control sows. Multiple linear regression was used to investigate associations between cell-mediated immunity and the magnitude of clinical signs. RESULTS: In 2 farms, case sows had lower ELISPOT assay values than control sows. A negative association between the intensity of the CMI response and the number of pigs born dead per litter was detected on 1 farm. In 1 farm, no association was detected between the intensity of the CMI response and protection against reproductive failure. CONCLUSIONS AND CLINICAL RELEVANCE: Evidence that a strong CMI response was correlated with protection against clinical PRRS was detected in 3 of 4 farms. However, farms and sows within farms varied considerably in their immune responsiveness and in the degree to which they were protected clinically. Increasing cell-mediated immunity within infected herds has the potential to decrease clinical reproductive disease, but only if the sources of intra- and interfarm variation in the intensity of cell-mediated immunity to PRRS virus can be identified.     

Swine reproductive and respiratory syndrome in Québec: Isolation of an enveloped virus serologically-related to Lelystad virus

Sera were collected from convalescent sows and sick piglets from six pig farms in southern Quebec that have experienced outbreaks of the so-called porcine reproductive and respiratory syndrome. By indirect immunoperoxidase, a few of these sera (4 of 14) (28.6%) were found to be positive for antibody to the Lelystad virus, whereas by indirect immunofluorescence 30 of 36 (83.3%) were positive for antibody to the antigenically-related American isolate ATCC-VR2332. Pregnant sows inoculated intranasally with filtered homogenates prepared from the lungs of necropsied piglets obtained from a seropositive farm developed fever, inappetence, and reproductive failure characterized by stillbirths and various stages of mummification. Lesions of interstitial pneumonia were induced in experimentally-infected specific pathogen-free piglets. A virus, having morphological and biological characteristics of viruses assigned to the family Togaviridae, was isolated from lung tissues of experimentally-infected animals; it could only be propagated in primary cultures of porcine alveolar macrophages. Identification of the virus was confirmed by indirect immunofluorescence using a monoclonal antibody directed against the nucleocapsid protein of the ATCC-VR2332 isolate and porcine sera that were found positive for antibody to both the Lelystad and ATCC-VR2332 isolates.     

一例猪圆环病毒2型和大肠杆菌、溶血葡萄球菌混合感染的诊断

山东德州某猪场发生猪高热、呼吸系统疾病甚至死亡的疫情。采集病料提取病变组织总DNA或RNA进行猪繁殖与呼吸综合征病毒、猪圆环病毒2型、猪细小病毒、猪伪狂犬病病毒、猪瘟病毒的PCR或RT-PCR检测。PCR扩增出353 bp的猪圆环病毒2型特异性条带。同时进行细菌分离培养、生化鉴定等试验,诊断为猪圆环病毒2型和大肠杆菌、溶血葡萄球菌混合感染。