能量与吡啶羧酸铬的互作效应对肥育猪肉质性状的影响

试验旨在研究能量与吡啶羧酸铬的互作效应对肥育猪肉质性状的影响。选择体重60 kg左右,杜×长×大三元杂交肥育猪36头,分为4组,分别饲喂低能日粮、低能+吡啶羧酸铬日粮、高能日粮、高能+吡啶羧酸铬日粮进行试验,对肥育猪背最长肌的肉色、pH值(屠宰后24 h)、大理石花纹、肌内脂肪(intramuscular fatI,MF)含量、剪切力、滴水损失、熟肉率、肌肉糖原含量指标进行测定。结果表明:①能量水平对肉色评分、滴水损失有极显著的影响(P<0.01);②能量水平对肌内脂肪(IMF)有显著影响(P<0.05);③能量水平对pH值、大理石花纹、剪切力、熟肉率、肌糖原含量均无显著影响(P>0.05);④添加吡啶羧酸铬对肉色评分、pH值I、MF、大理石花纹、滴水损失有极显著的影响(P<0.01);⑤添加吡啶羧酸铬对熟肉率、肌糖原含量有显著影响(P<0.05);⑥添加吡啶羧酸铬对剪切力无显著影响(P>0.05);⑦能量与吡啶羧酸铬互作对大理石花纹、熟肉率有显著影响(P<0.05);对其他肉质性状均无显著影响(P>0.05);⑧在低能日粮中添加200μg/kg吡啶羧酸铬,提高了肉色评分p、H值I、MF含量、大理石花纹和熟肉率;并降低了肌糖原含量。综上所述,在育肥后期采用低日粮能量水平并添加200μg/kg吡啶羧酸铬可改善肉质。     

吡啶羧酸铬和天冬氨酸镁对猪肉品质的影响

选择48头体重接近(86.0±5.1)kg的杜长大肥育阉公猪,研究天冬氨酸镁和吡啶羧酸铬对猪宰前应激肉品质的影响。试验猪分为4组,Ⅰ组为对照组,Ⅱ、Ⅲ、Ⅳ组为试验组,每组3个重复,每个重复4头,采食玉米-豆粕型日粮(DE,13.18MJ/kg;CP,14.6%)。Ⅰ组饲喂基础日粮;Ⅱ组饲喂基础日粮+1.0%天冬氨酸镁(MgAsp);Ⅲ组饲喂基础日粮+0.3mg/kg吡啶羧酸铬(Crpi);Ⅳ组饲喂基础日粮+1.0%MgAsp+0.3mg/kgCrpi。每头猪每日饲喂2.8kg上述试验日粮,7d后运到屠宰场。结果表明,日粮中补充吡啶羧酸铬、天冬氨酸镁、吡啶羧酸铬+天冬氨酸镁均降低了血清中皮质醇含量,减缓了屠宰前的应激反应;给宰前的育肥猪补充吡啶羧酸铬、吡啶羧酸铬+天冬氨酸镁均可降低宰后糖原分解(P0.05),补充天冬氨酸镁也有降低宰后糖原分解的趋势。补充吡啶羧酸铬、天冬氨酸镁及吡啶羧酸铬+天冬氨酸镁均可显著降低宰后短时间内猪背最长肌和股二头肌乳酸含量(P0.05);日粮中补充吡啶羧酸铬、天冬氨酸镁及吡啶羧酸铬+天冬氨酸镁均可极显著(P0.01)降低背最长肌和股二头肌pH和滴水损失,分别降低PSE肉发生率75.1%、49.8%和75.1%(P0.05),而对系水力无显著影响(P0.05));复合添加吡啶羧酸铬、天冬氨酸镁组猪的滴水损失、背最长肌和股二头肌色值都显著低于单独添加吡啶羧酸铬、天冬氨酸镁组,这说明吡啶羧酸铬、天冬氨酸镁在改善肉质指标方面具有一定的加性效应,二者同时添加效果最好。     

半胱胺和酵母铬对生长肥育猪生产性能和胴体品质的影响

试验选择25 kg左右的"杜长大"三元杂交猪96头,采用2×2析因设计分成4组,每组6个重复,每个重复4头。对照组喂基础饲粮,酵母铬(Cr)组在基础饲粮中添加酵母铬(生长期和肥育期分别添加Gr 200、300μg/kg),半胱胺(CS)组在基础饲粮中添加半胱胺(生长期和肥育期分别添加CS 120、170 mg/kg),复合组在基础饲粮中同时添加酵母铬和半胱胺(生长期和肥育期分别添加Gr 200μg+CS 120 mg/kg、Gr300μg+CS 170 mg/kg)。试验分生长期和肥育期两阶段进行,研究半胱胺和酵母铬对生长肥育猪生长性能、胴体和肌肉品质的影响。结果表明,生长肥育猪饲粮中分别添加半胱胺和酵母铬,一定程度上可改善生产性能,提高日增重、降低饲料/增重比;二者同时添加时有提高日增重的趋势,但统计分析差异不显著(P>0.05)。饲粮中分别或同时添加半胱胺和酵母铬对胴体肌肉品质也有一定的改善作用,但统计分析差异不显著(P>0.05)。     

吡啶羧酸铬对猪胴体品质的影响及其作用机理

选体重35 kg左右的杜长大杂种猪144头,按体重随机分成3组,分别喂以添加0、200、400 μg/kg吡啶羧酸铬的相同饲粮,试验期63 d.结果表明,与对照组比较,200μg/kg吡啶羧酸铬使肥育猪的日增重提高5.09%(P>0.05),400μg/kg吡啶羧酸铬使猪的采食量减少5.04%(P<0.05),两水平的吡啶羧酸铬均降低了料重比(P<0.05);瘦肉率、眼肌面积较对照组明显提高(P<0.05),脂肪率、背膘厚显著降低(P<0.05);血糖浓度、胰岛素水平明显下降(P<0.05),血清HDL、FFA浓度及脂肪酶活性显著升高(P<0.05);脂肪组织中的苹果酸脱氢酶、异柠檬酸脱氢酶活性显著下降(P<0.05),激素敏感脂酶的活性明显升高(P<0.05);下丘脑、腺垂体和脂肪组织中cAMP含量显著增加(P<0.05).     

蛋氨酸铬与其他有机铬对肥育猪生长性能影响的比较研究

为探讨蛋氨酸铬、吡啶羧酸铬及酵母铬对肥育猪生长性能的影响,试验选择96头杜×长×大三元杂交肥育猪,采用单因子随机方差试验设计,随机分为4组,每组3个重复,每重复8头猪。对照组采用基础日粮饲养,试验组在基础日粮中分别添加200μg/kg(以含铬量计算)蛋氨酸铬、吡啶羧酸铬和酵母铬,其余营养水平一致。试验结果表明:日粮中添加蛋氨酸铬、吡啶羧酸铬和酵母铬明显提高肥育猪的生产性能,增强机体抗病能力。蛋氨酸铬在提高肥育猪生长性能方面优于吡啶羧酸铬和酵母铬。     

吡啶羧酸铬对育成期笼养蛋鸭生长性能和血液生化指标的影响

试验旨在研究饲粮铬水平对育成期笼养蛋鸭生长性能、血液生化指标的影响。试验采用单因素完全随机分组设计,选择健康28日龄金定蛋鸭180只,随机分成5组,每组6个重复,每个重复6只鸭。对照组(Ⅰ组)饲喂玉米-豆粕型基础饲粮,试验组(Ⅱ～Ⅴ组)分别在基础饲粮中添加0.15、0.30、0.45、0.60 mg/kg铬(吡啶羧酸铬),试验期6周。结果表明:①在饲粮中添加铬(吡啶羧酸铬)对笼养蛋鸭的日增重影响差异显著(P<0.05),但对采食量和料重比影响差异不显著(P>0.05);②一定量的铬(吡啶羧酸铬)对笼养蛋鸭的血糖、总胆固醇、甘油三酯产生显著影响(P<0.05),并促进了血清总蛋白、白蛋白的提高,同时显著降低了血清尿素氮的含量(P<0.05)。最终通过二次曲线回归方程对添加量和测定指标结果进行回归分析,得出结论:铬(吡啶羧酸铬)在育成期笼养蛋鸭饲粮中最适宜的添加量为0.297 0～0.422 5 mg/kg。     

吡啶羧酸铬对热应激期奶牛体温、呼吸率、产奶量及乳成分的影响

试验旨在研究奶牛日粮中添加不同梯度的吡啶羧酸铬对热应激期奶牛体温、呼吸率、产奶量和牛乳中铬及乳成分的影响,为吡啶羧酸铬在奶牛热应激期的安全应用提供依据。选择20头荷斯坦泌乳牛,采用单因子区组设计,分为对照组和处理Ⅰ、Ⅱ、Ⅲ三个试验组。对照组饲喂基础日粮,试验Ⅰ、Ⅱ、Ⅲ组在此基础上分别添加吡啶羧酸铬4、8和12mg/(头·d),试验期60d。结果表明,奶牛在热应激状态下,日粮中添加吡啶羧酸铬4、8、12mg/(头·d)对牛奶中铬含量影响不显著(P>0.05);对牛奶常规成分(乳脂率、乳蛋白率、乳糖率、总固形物)无显著影响(P>0.05);添加8、12mg/(头·d)水平的吡啶羧酸铬对减缓奶牛热应激、提高产奶量有显著作用(P<0.05)。     

复合酶对生长肥育猪生长性能、胴体性状及部分肉品质的影响

试验旨在研究日粮中添加复合酶对生长肥育猪生长、胴体性状及部分肉品质的影响。试验选择60头日龄、胎次相近,体重在60 kg左右的杜×大×长三元肉猪,随机分成对照组、复合酶Ⅰ组、复合酶Ⅱ组及阳性对照组,每组15头。试验期为58 d,在相同饲养管理条件下,对照组饲喂基础日粮,复合酶Ⅰ组饲喂基础日粮+1.0‰复合酶,复合酶Ⅱ组饲喂基础日粮+2.0‰复合酶,阳性对照组饲喂基础日粮+0.2‰酶制剂。试验表明:①生长性能方面,复合酶Ⅰ组试末均重(BW)、平均日增重(ADG)显著高于其它各组(P<0.05),料重比(F/G)显著低于其它各组(P<0.05),BW、ADG比对照组分别提高了4.9%、12.45%,F/G降低了11.2%;②胴体性状方面,各组间屠宰率、胴体重、胴体长、皮率及骨率差异不显著(P>0.05);复合酶Ⅰ组的背膘厚显著低于对照组(P<0.05)、瘦肉率显著高于对照组和阳性对照组(P<0.05),皮厚、板油重及肥肉率略低于对照组(P>0.05)),眼肌面积略高于对照组(P>0.05);③肉品质方面,各组间肉的pH值、L*值及a*值差异不显著(P>0.05),但与对照组相比,复合酶Ⅰ组有提高的趋势;复合酶Ⅰ组肉的失水率显著低于对照组(P<0.05),大理石纹评分显著高于对照组(P<0.05)。结果表明,日粮中添加复合酶可改善生长肥育猪的生长性能、胴体性状和肉品质,以1.0‰添加效果最好。     

包膜半胱胺对肥育猪生长性能、免疫功能、抗氧化能力及肠内容物消化酶活性的影响

本试验旨在研究日粮中添加不同水平包膜半胱胺(CCS)对肥育猪生长性能、免疫和抗氧化能力及十二指肠消化酶活性的影响。选择体重(62.12±1.43)kg的健康杜×长×大肥育猪160头,随机分为4个处理,每个处理组4个重复,每个重复10头(公、母各半)。对照组饲喂基础日粮,试验组在基础日粮的基础上分别添加100、200、400 mg/kg CCS。试验猪自由采食饮水,预试期5 d,正试期40 d。结果表明:CCS对肥育猪的平均日增重(ADG)、平均日采食量(ADFI)和耗料增重比(F/G)无显著影响,但100 mg/kg CCS组生长性能指标相对最佳;100、200 mg/kg CCS组血清IgM和补体C3水平均上升(P<0.05),C3水平随着CCS添加剂量的提高呈线性上升(P<0.05);CCS有提高血清Ig G水平的趋势(P=0.07),并呈二次升高(P<0.05);CCS提高了血清中总抗氧化能力(T-AOC)并呈二次变化(P<0.05);血清谷胱甘肽过氧化物酶(GSH-Px)水平和过氧化氢酶(CAT)活性随着CCS水平的提高呈线性及二次变化(P<0.05);肝脏GSH-Px和总超氧化物歧化酶(T-SOD)活性随着CCS水平的提高呈线性及二次增高(P<0.05);CCS提高了十二指肠总蛋白酶和总脂肪酶活性(P<0.05),使总淀粉酶活性呈二次上升(P<0.05)。可见,日粮中添加低剂量的CCS对肥育猪生产性能有一定改善趋势,CCS可提高肥育猪的免疫和抗氧化性能,促进肠道消化酶的分泌。在本试验中,CCS在肥育猪饲粮中的适宜添加水平为100 mg/kg。     

羧酸吡啶铬对肥育猪胴体组成及肉质的影响

取体重６０ｋｇ左右健康“杜长宁”三元杂交肥育猪６０头,按饲养试验要求分成对照和试验２个处理组,饲喂相同的玉米－豆粕型日粮,其中对照组不添加羧酸吡啶铬,而试验组添加２００μｇ／ｋｇ羧酸吡啶铬。结果表明：①羧酸吡啶铬能明显改善肥育猪的胴体组成。与对照组相比,试验组胴体瘦肉率和眼肌面积分别提高了７．２６％（Ｐ〈０．０５）和７．０９％（Ｐ〈０．０５）;脂肪率、板油重和平均背膘厚度分别降低了１２．７７％（Ｐ     

癸氧喹酯干混悬剂含量测定的改进

采用高效液相色谱法测定癸氧喹酯干混悬剂的含量,在2-250μg/mL范围内,峰面积的常用对数与进样量浓度的常用对数呈良好的线性关系,R^2=1(n=5),平均回收率为99.24%~99.51%,RSD在0.05%~0.28%。此方法分析时间短,样品前处理简便、定量结果准确,重现性好,结果满意,为其质量控制提供了依据。     

商会自治的基石——商会自治规范研究

在现代法律秩序中,商会自治规范是制定法的基础和必要的补充,甚至在某些方面替代了制定法;商会自治规范主要包括商会组织规范、行为规范、惩罚规范以及争端解决规范等;其效力仅及于其内部成员;商会自治规范和制定法之间存在冲突,但也存在整合的基础。     

猪毛色遗传的研究进展

本文概述了猪的毛色类型、猪的毛色遗传模式,着重综述了猪毛色基因分子基础的研究进展,指出存在问题并就未来发展方向做了思考。     

中华人民共和国农业部公告 第267号

为贯彻落实《兽药生产质量管理规范》(简称《兽药GMP》),进一步推动兽药GMP实施进程,我部制定了《兽药生产质量管理规范检查验收办法》,现予公告。本公告自2003年6月1日起施行。附件:兽药生产质量管理规范检查验收办法二○○三年四月十日第一章 总则 第一条 为推动《兽药生产质量管理规范》(以下简称兽药GMP)的实施,规范兽药GMP检查验收工作,制定本办法。 第二条 农业部负责全国兽药GMP管理和检查验收工作;负责制修订兽药GMP检查验收管理规定;负责兽药GMP检查员队伍建设和监督管理工作,负责国际兽药贸易中GMP互认工作。 …     

鸡败血支原体垂直传播模式及其阻断方法

以国际标准强毒Ｒ株人工感染非免疫产蛋鸡,定时扑杀,分别从鼻窦、眶下孔、气管、肺、气囊、卵巢和输卵管分离ＭＧ,并收集感染鸡所产蛋分离ＭＧ。结果表明,人工感染４８小时后上、下呼吸道及肺已被全面感染,９６小时气囊已被感染,１２０小时输卵管已能分离到ＭＧ,卵巢始终分离不到ＭＧ。人工感染鸡自１４４小时便能在其所产蛋中分离出ＭＧ。药物治疗能在７２小时内消除感染,油乳剂苗则需２４天后逐渐降低蛋内ＭＧ分离率,药物卵内注射、种蛋药浴、高温处理均能杀死卵内ＭＧ,但以研制的种蛋浸泡剂药浴效果为最好。     

Comparison of 2 methods of centesis of the bursa of the biceps brachii tendon of horses

REASONS FOR PERFORMING STUDY: Centesis of the bicipital bursa using an 8.9 cm long spinal needle has been reported but the alternative of employing a 3.8 cm long hypodermic needle requires validation. OBJECTIVE: To compare the efficacy of 2 different methods of centesis of the bicipital bursa and to evaluate the usefulness of ultrasonographic imaging to determine the location of solution administered when centesis of the bursa is attempted. METHODS: For Trial 1, 6 clinicians, who had no previous experience of centesis of the bicipital bursa, attempted to inject a solution composed of an aqueous radiopaque contrast medium and physiological saline solution (PSS) into the bicipital bursae of 2/12 horses using the previously described distal approach to inject one bursa and a proximal approach to inject the contralateral bursa. The bicipital tendon and bursa were examined ultrasonographically before and after injection; and both shoulders were examined radiographically to identify the location of the medium. In Trial 2, another 6 clinicians, also with no previous experience of centesis, repeated Trial 1, using 6 horses, but the radiopaque contrast medium was mixed with air instead of PSS. RESULTS: Accuracy of centesis using the proximal approach was 39% and that of the distal approach 28%. Ultrasonographic examination of the shoulder allowed the location of solution and air to be accurately predicted in all 12 shoulders examined. CONCLUSIONS: Clinicians who have had no previous experience performing centesis of the bicipital bursa are unlikely to be successful in centesis using either approach. Radiographic examination after injecting a radiopaque contrast medium may be necessary to assess the success of centesis especially if bursal fluid is not obtained during centesis. Injecting air along with the radiopaque contrast medium provides more accurate ultrasonographic confirmation of centesis and better radiographic definition than does injection without air.     

不同样本商品蜂蜜中硒含量的测定

用硝酸和高氯酸消化蜂蜜,使硒游离出来,在微酸性环境下,硒和2,3-二氨基萘(DAN)生成有较强荧光的物质,用环己烷萃取,在激发波长378nm,荧光波长518nm处测定其荧光强度。蜂蜜中硒含量范围:0.10～0.82μg/g。表明:蜂蜜应视为天然富硒营养品。     

乳酸杆菌益生作用机制的研究进展

乳酸杆菌作为益生菌广泛用于人和动物。本文综述了乳酸杆菌改善宿主健康的机制。乳酸杆菌可通过产生抗菌物质如乳酸、过氧化氢、细菌素,或者通过竞争营养或肠道黏附位点来抑制致病菌;通过诱导黏附素的分泌或阻止细胞凋亡而增强肠道的屏障功能,从而保护肠道。文章重点讨论了乳酸杆菌表面成分（表面蛋白、脂磷壁酸和肽聚糖）与肠道受体（Ｃ型凝集素受体、Ｔｏｌｌ样受体和 Ｎｏｄ样受体）,阐述了他们结合后启动免疫调节信号,调控肠道免疫功能以发挥改善健康作用的机制。     

Effects of size of ingestively masticated fragments of plant tissues on kinetics of digestion of NDF

Ingestively masticated fragments were collected and sized via sieving. Different sizes of esophageal masticate and ruminal digesta fragments, and ground fragments of larger masticated pieces were incubated in vitro, and undigested NDF remaining at intervals of up to 168 h of incubation was determined. The ruminal age-dependent time delay (tau) for onset of digestion of NDF was positively correlated (P < 0.004) with the mean sieve aperture estimated to retain 50% of the fragments between successive sieve apertures (MRA). Degradation rate of potentially degradable NDF (PDF) and level of indigestible NDF were not related (P > 0.10) to MRA of masticated and ground fragments. Estimates of tau were positively related to MRA, with slopes of bermudagrass < corn silage < ruminal fragments of corn silage. It was concluded that fragment size-, and consequently, ruminal age-dependent onset of PDF degradation of a mixture of different fragment sizes results in an age-dependent rate of degradation of the more rapidly degrading of two subentities of PDF. Models are proposed that assume a tau before onset of simultaneous degradation of PDF from two pools characterized as having gamma-modeled age-dependency and age-constant rates. The ruminal age-dependent pool seems to be associated with the faster-degrading pool, and its rate parameter increases with range in MRA in the population of fragments. Conceptually, the ruminal age-dependent rate parameter for PDF degradation seems to represent a composite of several effects: 1) effects of the size-dependent tau; 2) range in MRA of the population of ingestively masticated fragments; and 3) subentities of PDF that degrade via more rapid age-dependent rates compared with subentities of PDF that degrade via age-constant rates. The estimated fractional rates of ruminative comminution of ingestively masticated fragments (0.060 to 0.075/h) were of a magnitude similar to the mean fractional rates of PDF digestion (0.030 to 0.085/h), which implies that ruminative comminution may be first-limiting to fractional rate of PDF digestion. The in vivo roles of ingestive and ruminative mastication of fragments on PDF degradation must be considered in any kinetic system for estimating PDF digestion in the rumen. These results and others in the literature suggest that the rate of surface area exposure rather than intrinsic chemical attributes of PDF may be first-limiting to degradation rate of PDF in vivo.