猪瘟病毒、猪繁殖与呼吸综合征病毒、支原体混合感染病例的实验室诊断

为查明贵州省贵定县某养殖场猪群发病的原因,分别采集2头病猪的肺脏、肝脏、扁桃体、淋巴结、脾脏、肾脏组织进行细菌分离培养及猪瘟病毒、猪繁殖与呼吸综合征病毒、支原体的核酸检测。结果:无细菌感染情况;猪瘟病毒、猪繁殖与呼吸综合征病毒、支原体核酸检测均为阳性。结论:养殖场病例存在猪瘟病毒、猪繁殖与呼吸综合征病毒、支原体混合感染。     

猪圆环病毒感染的PCR鉴别诊断

为了确诊临床疑似猪圆环病毒感染病例,为猪场疫病的防控与净化明确目标,采集病例组织样本运用RT-PCR和PCR方法进行猪瘟病毒、猪繁殖与呼吸综合征病毒、猪支原体、猪圆环病毒检测。结果:猪瘟病毒、猪繁殖与呼吸综合征病毒、猪支原体检测为阴性,猪圆环病毒检测为阳性,确诊为猪圆环病毒感染。     

猪繁殖与呼吸综合征病毒和猪瘟病毒混合感染的检测

2005年3月,江苏某猪场仔猪发生体温升高,呼吸困难,四肢末端、耳尖发绀,站立不稳和淋巴结出血为主要症状的疾病。4头发病仔猪的淋巴结、脾、肺脏组织用RT-PCR方法分别检测猪繁殖与呼吸综合征病毒和猪瘟病毒为阳性,用猪瘟ELISA试剂盒检测猪瘟病毒野毒为阳性。结合本病的临床症状和病理剖检,病例确诊为猪繁殖与呼吸综合征和猪瘟野毒的混合感染。     

猪瘟、猪繁殖与呼吸综合征混合感染的诊断与防制探讨

猪瘟、猪繁殖与呼吸综合征是全球性的高危害传染性疾病,严重危害我国的养殖业发展,并阻碍我国养猪行业的经济发展水平。由于多种因素影响,我国的养猪行业的发展一直存在阻碍,而猪瘟、猪繁殖与呼吸综合征等动物疾病正是影响我国养猪行业发展的重要原因之一。猪瘟和猪繁殖与呼吸综合征会严重影响生猪的身体健康,使母猪产生繁殖障碍,还会导致生猪的死亡。本文对猪瘟和猪繁殖与呼吸综合征混合导致猪感染的特征进行探讨和分析,并制定有效的疾病防制对策,保障养猪业的顺利发展。     

猪瘟和猪蓝耳病感染的诊断与防控

猪瘟是一种具有高度传染性疫病,是威胁养猪业的主要传染病之一.对某养猪户饲养的猪发生的一起疫病,根据流行病学调查、剖检变化、实验室检验等诊断为猪瘟病毒、猪繁殖与呼吸综合征病毒的混合感染,并提出了防控措施.     

猪瘟、口蹄疫、猪繁殖与呼吸综合征等多种疫苗免疫试验

将猪瘟、猪口蹄疫、猪繁殖与呼吸综合征3种疫苗进行不同组合,采用不同的免疫程序,即分别进行单独注射、3种疫苗同时注射、两两不同组合注射,以及根据我省在集中免疫时同时注射猪三联疫苗的实际情况,设置了一组4种疫苗同时注射组,研究其抗体影响规律,探索既提高工作效率,又提高免疫质量的多种疫苗有效结合的新型免疫程序。试验分为9组,每组30头,用ELISA方法和正向间接血凝试验检测免疫抗体。结果表明:口蹄疫、猪瘟、猪繁殖与呼吸综合征和猪三联分点同时注射组产生的口蹄疫抗体水平要优于其他组,其中猪瘟抗体水平优于单苗注射组和其他两两组合组,猪繁殖与呼吸综合征抗体与单苗注射组间无明显差异。在两两不同组合组间,口蹄疫和猪繁殖与呼吸综合征组合后,对口蹄疫抗体的产生没有影响;猪繁殖与呼吸综合征和猪瘟组合,间隔1周后注射口蹄疫疫苗,口蹄疫抗体值较低;对于猪瘟抗体而言,只要先注射猪瘟疫苗,间隔1周后注射其他疫苗,都使猪瘟抗体产生的时间推迟了2周左右;猪繁殖与呼吸综合征单苗注射组抗体阳性率与猪繁殖与呼吸综合征和口蹄疫组合组抗体阳性率值相同,而猪繁殖与呼吸综合征和猪瘟组合后,抗体值稍低于单苗注射组。     

猪圆环病毒2型和猪繁殖与呼吸综合征病毒混合感染病例的实验室诊断

为确诊贵阳市某养猪场生猪发病死亡的原因,采集3头病猪的血液、肺脏、肾脏、脾脏、淋巴结病料,采用PCR、qRT-PCR方法进行猪圆环病毒2型、猪繁殖与呼吸综合征病毒、猪瘟病毒核酸检测。结果:在血液及组织样品中猪圆环病毒2型核酸检测均为阳性,猪瘟病毒核酸检测均为阴性;在血液样品中猪繁殖与呼吸综合征病毒核酸检测结果为阴性,而组织样品中检测结果为阳性。结论:猪场病例存在猪圆环病毒2型、猪繁殖与呼吸综合征病毒混合感染。与血液样品相比较,猪繁殖与呼吸综合征病毒核酸在病猪的组织病料中更容易检出。     

一步法RT-PCR同时检测CSFV和PRRSV方法的建立及应用

猪瘟(CSF)和猪繁殖与呼吸综合征(PRRS)是严重危害云南养猪业的传染病,为了建立一种能快速诊断猪瘟和猪繁殖与呼吸综合征混合感染的方法,根据NCBI上已发表的猪瘟病毒(CSFV)保守基因Erns和猪繁殖与呼吸综合征病毒(PRRSV)保守基因M基因的核苷酸序列,利用Oligo 6.0设计了2对特异性引物,经过PCR反应...     

一起猪附红体病、弓形虫病、链球菌病混合感染的诊治

2009年1月,某养猪户饲养的仔猪发病.其共有存栏仔猪120余头,体重20～50 kg,日粮包括米皮、玉米粉和一些预混剂.饲养仔猪均免疫接种过伪狂犬病、气喘病、口蹄疫、猪瘟和猪繁殖与呼吸综合征等疫苗;其中,对口蹄疫、猪瘟和猪繁殖与呼吸综合征都进行了二免;此外,在3日龄时均注射了生血素,并用长效土霉素进行了"三针保健",即在仔猪1日龄、7日龄、21日龄时分别注射0.5、1和1 mL.     

河南省部分猪场猪繁殖与呼吸综合征和猪瘟血清抗体检测

为了解河南省部分猪场猪繁殖与呼吸综合征和猪瘟的抗体保护水平,2018年采用ELISA方法,对河南省部分一级监测网点猪场开展了猪繁殖与呼吸综合征和猪瘟血清抗体检测。检测结果显示:猪繁殖与呼吸综合征抗体总阳性率为91.28%,猪瘟抗体总阳性率为95.87%。其中:后备猪、种公猪、经产母猪、保育猪、育肥猪血清样品的猪繁殖与呼吸综合征抗体阳性率分别为93.68%、93.87%、92.16%、88.93%、88.65%,猪瘟抗体阳性率分别为98.51%、98.77%、98.45%、85.71%、91.82%;祖代场、父母代场、商品场血清样品的猪繁殖与呼吸综合征抗体阳性率分别为94.63%、88.02%、89.46%,猪瘟抗体阳性率分别为98.40%、96.16%、91.03%。检测结果表明:河南省一级监测网点猪场的猪繁殖与呼吸综合征和猪瘟抗体水平较高,但保育猪群和育肥猪群偏低,需要对其加强抗体监测,以确定最佳免疫方案;商品代猪场抗体水平略低于祖代和父母代猪场,这要求畜牧兽医部门需同时加强对所有猪场的日常监督与监管。     

一例猪圆环病毒2型和大肠杆菌、溶血葡萄球菌混合感染的诊断

山东德州某猪场发生猪高热、呼吸系统疾病甚至死亡的疫情。采集病料提取病变组织总DNA或RNA进行猪繁殖与呼吸综合征病毒、猪圆环病毒2型、猪细小病毒、猪伪狂犬病病毒、猪瘟病毒的PCR或RT-PCR检测。PCR扩增出353 bp的猪圆环病毒2型特异性条带。同时进行细菌分离培养、生化鉴定等试验,诊断为猪圆环病毒2型和大肠杆菌、溶血葡萄球菌混合感染。     

三明市某规模化猪场几种疫病的检测情况与分析

通过对三明市某大型猪场进行猪瘟、普通蓝耳病、高致病性蓝耳病、伪狂犬病的病原学和血清学检测,以更好地了解这些疫病在猪场的流行情况,为猪场这几种疫病的防控和净化奠定基础。本次调查分别检测45份后备母猪血清、92份生产母猪血清、13份生产公猪血清,共计150份血清,150份扁桃体样品。通过ELISA方法分别检测了猪瘟、普通蓝耳病、伪狂犬病gE和伪狂犬病gB。荧光定量PCR方法检测了猪瘟、高致病性蓝耳病。结果猪瘟和高致病性蓝耳病病原学阳性率分别为8%和5.3%。一个月后对病原学检测阳性猪采样复查,结果猪瘟阳性率4.6%,高致病性蓝耳病阳性率2.6%。猪瘟免疫抗体合格率93.3%,普通蓝耳病免疫抗体合格率96%,伪狂犬病gB免疫抗体合格率100%,伪狂犬病gE感染抗体2%。生产母猪胎次越多,猪瘟和普通蓝耳病的免疫抗体水平越高。     

Clinical signs and epidemiology of classical swine fever: a review of new knowledge

Although classical swine fever (CSF) has been well known for decades and epidemics still occur, clinical diagnosis continues to cause problems for veterinary practitioners. This is due to the extensive differential diagnosis, further complicated by the emergence of new diseases such as porcine reproductive and respiratory syndrome (PRRS) and porcine dermatitis and nephropathy syndrome (PDNS). In addition, acute, chronic and prenatal courses of CSF have to be distinguished. As a cause of considerable economical losses within the EU, control of CSF requires knowledge of the primary outbreaks and spread of the disease. Genetic typing of CSF virus isolates has proved to be a potent method of supporting epidemiological investigations. Phylogenetic analysis of CSF virus strains and isolates originating from different continents has allowed three genetic groups and several subgroups within these groups to be distinguished. Whereas isolates belonging to group 3 seem to occur solely in Asia, all CSF virus isolates of the 1990s isolated in the EU belonged to one of the subgroups within group 2 (2.1, 2.2, or 2.3) and were clearly distinct from former CSF reference viruses, which belong to group 1. Within the EU, different strategies are followed for the eradication of CSF in domestic pigs and in wild boar. While a strict non-vaccination policy is followed for domestic pigs, eradication of the disease in wild boar is more complex, and oral immunisation together with special hunting strategies have been applied. Recently, marker vaccines with a companion discriminatory test designed to allow differentiation between vaccinated animals and animals having recovered from field virus infection have been developed. Preliminary studies indicated that the discriminatory tests had a reduced sensitivity and specificity. Further improvements are therefore necessary before marker vaccines can be considered for emergency use in EU Member States. Prevention of CSF remains the main objective within the EU.     

A review of viral diseases of the European wild boar: effects of population dynamics and reservoir rôle

There has been a worldwide increase in the number and geographical spread of wild boar populations in recent decades leading to an increase in both the circulation of disease agents and greater contact with domestic animals and humans. Diseases affect the population dynamics of wildlife but the effects of most viral diseases on the European wild boar are largely unknown. Many viral diseases present in domestic pig populations are also present in wild boars where they can provide a disease reservoir, as is clearly the case with classical swine fever, but little is known about other viral diseases such as porcine circovirus diseases or hepatitis E. This review considers the current scientific knowledge of the effects of viral diseases on wild boar populations and their r?le as potential disease reservoirs. The focus is on those viral diseases of domestic swine and wild boars that are included as notifiable by the Office International des Epizooties (OIE).     

Prevalence of antibodies to classical swine fever,Aujeszky's disease,porcine reproductive and respiratory syndrome,and bovine viral diarrhoea viruses in wild boars in Croatia

During the hunting season in February 1999, a total of 44 blood samples were collected from wild boars shot in the area of Moslavacka gora. These blood samples were examined by enzyme immunoassay for the presence of antibodies to classical swine fever (CSFV), Aujeszky's disease (ADV), bovine viral diarrhoea (BVDV), and porcine reproductive and respiratory syndrome (PRRSV) viruses. Out of 44 serum samples examined, 17 (38.63%) were positive for CSFV, 24 (54.54%) were positive for ADV and two (4.54%) were positive for BVDV. All sera were negative for PRRSV. The results, recorded for the first time in Croatia, supported the hypothesis that wild boar act as a potential reservoir of CSFV, ADV and BVDV, and thus have a role in the epidemiology of these diseases.     

某规模化猪场伪狂犬病的诊断

河南平顶山某猪场母猪出现较严重的流产和产死胎现象,且50日龄~70日龄仔猪出现神经症状,根据临床表现初步诊断为伪狂犬病。为排除猪繁殖与呼吸综合征和猪瘟,进行了实验室诊断。应用ELISA方法检测发病保育猪及母猪血清的伪狂犬病病毒野毒株gE抗体,并对发病仔猪病料进行了伪狂犬病病毒(PRV)、猪繁殖与呼吸综合征病毒(PRRSV)和猪瘟病毒(CSFV)的实时荧光定量PCR检测。结果显示,伪狂犬病病毒野毒抗体阳性,实时荧光定量PCR检测确定仔猪病料中PRV核酸阳性,PRRSV和CSFV核酸阴性。结合临床症状及实验室检测,确诊该猪场发生的是猪伪狂犬病。     

Prevalence of Antibodies to Classical Swine Fever,Aujeszky's Disease,Porcine Reproductive and Respiratory Syndrome,and Bovine Viral Diarrhoea Viruses in Wild Boars in Croatia

During the hunting season in February 1999, a total of 44 blood samples were collected from wild boars shot in the area of MoslavaC?ka gora. These blood samples were examined by enzyme immunoassay for the presence of antibodies to classical swine fever (CSFV), Aujeszky's disease (ADV), bovine viral diarrhoea (BVDV), and porcine reproductive and respiratory syndrome (PRRSV) viruses. Out of 44 serum samples examined, 17 (38.63%) were positive for CSFV, 24 (54.54%) were positive for ADV and two (4.54%) were positive for BVDV. All sera were negative for PRRSV. The results, recorded for the first time in Croatia, supported the hypothesis that wild boar act as a potential reservoir of CSFV, ADV and BVDV, and thus have a role in the epidemiology of these diseases.     

2019年猪病流行情况与2020年流行趋势及防控对策

文章结合实验室的监测数据,概述了2019年我国非洲猪瘟、猪繁殖与呼吸综合征、猪瘟、猪伪狂犬病、猪流行性腹泻等重要猪病的流行状况,着重总结了非洲猪瘟疫情发生情况。分析了2020年非洲猪瘟等疫病的流行趋势,同时提出了坚持生物安全、严格处置非洲猪瘟疫情等防控策略。     

养殖场非洲猪瘟防控措施及实践

2018年8月3日,农业农村部发布辽宁沈阳发生全国首例非洲猪瘟。至今,全国32个省、自治区、直辖市、特别行政区公布共发生149起非洲猪瘟疫情,超过116万头猪只被扑杀。面对前所未有的非洲猪瘟,目前尚无有效的药物和疫苗预防,养猪行业面临种种危机。文章对非洲猪瘟及其危害做了简要介绍,并提出了防控非洲猪瘟的措施和建议。     

共表达猪繁殖与呼吸综合征病毒GP5基因和猪瘟病毒E2基因的重组腺病毒的构建及其在小鼠模型上的免疫原性分析

本研究旨在以复制缺陷型人5型腺病毒为载体,构建一株同时表达猪繁殖与呼吸综合征病毒(PRRSV)变异株GP5蛋白和猪瘟病毒(CSFV)E2蛋白的重组腺病毒疫苗。首先利用重叠PCR将GP5和E2基因通过口蹄疫病毒(FMDV)2A序列连接,形成一个完整的ORF,并将其克隆到腺病毒穿梭载体中,通过细菌内同源重组构建共表达PRRSVGP5蛋白和CSFVE2蛋白的重组腺病毒(rAdV-GP52AE2)。间接免疫荧光试验和western blot检测证实2个外源基因均获得表达。在小鼠上进行的免疫效力评价结果显示,rAdV-GP52AE2免疫组针对CSFV的中和抗体滴度可达1∶128,针对PRRSV的中和抗体滴度为1∶16;在淋巴细胞增殖试验中,免疫组与阴性对照组在增殖指数上有显著差异,表明该重组腺病毒可以同时诱导抗PRRSV和CSFV的特异性体液免疫和细胞免疫。这些结果显示,利用具有自动剪切功能的FMDV2A多肽构建的重组腺病毒有望开发成一种同时预防PRRS和CSF的新型载体疫苗。