2019年山东省兽医系统实验室检测能力比对

2019年山东省动物疫病预防控制中心组织全省98个兽医系统实验室开展检测能力比对工作,比对项目包括非洲猪瘟病毒核酸检测、禽流感病毒核酸检测、H5亚型禽流感病毒抗体检测和口蹄疫非结构蛋白抗体检测。比对结果显示:总体实验室正确率为70.41%(69/98),其中市级兽医系统实验室正确率为93.75%(15/16),国家动物疫情测报站正确率为68.75%(11/16),县级兽医系统实验室正确率为65.15%(43/66);非洲猪瘟病毒核酸检测、禽流感病毒核酸检测、H5亚型禽流感病毒抗体检测以及口蹄疫非结构蛋白抗体检测结果正确率分别为87.76%、85.71%、93.88%和97.96%。比对结果提示基层兽医系统实验室应提升检验人员的素质,加大实验室设施设备的投入和管理,结合开展社会化服务来推进实验室建设,确保检测结果客观准确。     

2022年湖南省兽医实验室检测能力比对及非洲猪瘟检测能力验证

实验室检测能力比对与能力验证是评估实验室检测质量的重要手段。2022年4月,按照农业农村部关于检测能力比对的工作部署和要求,湖南省组织全省14个市级77个县级兽医系统实验室开展了检测能力比对,267个实验室开展了非洲猪瘟能力验证。市级开展禽流感病毒H5/H7亚型核酸鉴别检测、非洲猪瘟病毒核酸检测、O型口蹄疫病毒抗体检测3个项目,县级开展非洲猪瘟病毒核酸、O型口蹄疫病毒抗体以及布鲁氏菌抗体检测3个项目,非洲猪瘟检测实验室开展非洲猪瘟病毒核酸检测项目,每个项目各5份盲样。结果显示:12个市级和59个县级系统实验室所有比对项目检测结果全部准确,实验室整体准确率为78.02%,1 355份样品中1 317份样品检测结果准确,样品整体准确率为97.20%;267个参加非洲猪瘟能力验证的实验室中,224个实验室核酸检测结果准确,实验室整体准确率为83.90%,1 335份样品中1 265份样品结果准确,样品整体准确率为94.76%,其中屠宰企业样品准确率最低(93.30%)。结果表明:湖南省兽医实验室检测能力整体良好,但部分县级兽医系统实验室及屠宰企业实验室检测能力有待加强。各地应继续加强实验室检...     

2014年辽宁省市县兽医系统实验室检测能力比对结果分析

2014年,对全省14个市和58个县级兽医实验室开展了检测能力比对,比对项目包括H7N9亚型流感抗体检测、猪瘟抗体检测、高致病性猪蓝耳病病毒核酸检测、鸡白痢抗体检测。比对结果表明,有11个市级实验室和47个县级实验室比对结果全部正确。其中H7N9亚型流感抗体检测项目实验室正确率为95.77%,猪瘟抗体检测项目实验室正确率为100%,高致病性猪蓝耳病病毒核酸检测项目实验室正确率为75.68%,鸡白痢抗体检测项目实验室正确率为100%。比对结果说明市县级兽医实验室HI、ELISA和平板凝集方法检测抗体的技术掌握较好,而用荧光RT-PCR检测病毒核酸的技术能力有待提高。     

2016～2020年内江市兽医实验室检测能力比对结果分析

为确保兽医实验室检测结果的科学性和准确性,内江市市级兽医实验室积极参与全省兽医系统实验室检测能力比对试验。2016～2020年,开展了16个比对项目检测,项目类型包含:禽流感病毒抗体检测、口蹄疫病毒抗体检测、猪瘟病毒抗体检测、禽流感病毒核酸检测、口蹄疫病毒核酸检测、非洲猪瘟病毒核酸检测。结果显示:内江市兽医实验室比对结果 5年的准确率均为100%。结果表明,内江市兽医实验室检测水平较高,稳定性也较好,具备较强实验室检测能力。     

2021年青海省兽医系统实验室检测能力比对结果分析

为评估青海省兽医系统实验室检测能力，2021年9月，青海省动物疫病预防控制中心组织全省开展能力比对工作，共有8个市（州）、39个县（市）的47个兽医实验室参加，分别开展了非洲猪瘟病毒核酸检测、高致病性禽流感H5/H7亚型鉴别诊断、口蹄疫病毒O型抗体检测、布鲁氏菌抗体检测和细菌染色镜检。全省8个市（州）、39个县（市）动物疫控中心兽医实验室全部参加了能力比对，共检测盲样700份，检测结果准确559份，总准确率为79.86%(559/700)，其中准确率为100%的实验室有12个，占参与比对实验室总数的25.53%(12/47)。结果表明，青海省兽医系统实验室检测能力总体良好，部分县（市）级兽医实验室因人员不固定、基础设施薄弱、资金支持不足等原因导致检测能力较弱。     

生猪屠宰场非洲猪瘟检测能力比对

为了了解山西省屠宰场非洲猪瘟的检测能力与水平,2019年山西省动物疫病预防控制中心对全省11个市的屠宰场开展了非洲猪瘟病毒检测能力比对。本次比对采用荧光PCR检测方法进行非洲猪瘟病毒核酸检测。结果显示,共有96个屠宰场参与比对工作,其中71个屠宰场检测结果符合要求,符合率为74.0%。比对结果客观地反映了全省屠宰场非洲猪瘟病毒核酸检测能力,表明屠宰场非洲猪瘟病毒核酸检测工作仍存在多方面不足,应该进一步增强检测人员的业务能力,以确保检测结果真实准确。     

2022年四川省市级兽医系统实验室布病检测能力比对分析

为评估四川省内兽医系统实验室对布鲁氏菌病的检测能力，我们于2022年开展了全省市级兽医实验室比对工作，共20个市级兽医系统实验室参与比对。布鲁氏菌病抗体检测项目通过虎红平板凝集试验比对，11个市（州）使用A厂家试剂，9个市（州）使用B厂家试剂。结果得出：布鲁氏菌病抗体检测项目结果总体符合率为68.75%,A厂家符合率为81.82%,B厂家符合率为52.78%。本研究表明，四川省市级兽医系统实验室对动物布鲁氏菌病的检测能力有待提高，实验试剂对实验结果的影响很大。     

2016—2020年四川省内江市兽医系统实验室检测能力比对

为提升四川省内江市各兽医实验室检测能力和水平,确保兽医实验室检测结果的科学性、准确性和一致性,2016—2020年内江市动物疫病预防控制中心连续组织开展了全市兽医系统实验室检测能力比对工作。比对项目涵盖高致病性禽流感、口蹄疫、高致病性猪繁殖与呼吸综合征和猪瘟抗体检测以及非洲猪瘟病毒核酸检测。结果显示,各年度实验室比对结果平均准确率分别为100%、94.7%、100%、85.3%、96.7%;荧光聚合酶链式反应、酶联免疫吸附试验、血凝及血凝抑制试验3个检测项目比对准确率分别为100%、96.6%、91.5%。结果表明,内江市兽医系统实验室总体检测水平较高,但稳定性欠佳,实验室能力建设有待进一步加强。     

2018—2020年四川省兽医系统实验室检测能力比对

为评估四川省兽医系统实验室检测能力和水平，2018—2020年四川省动物疫病预防控制中心组织全省21个地市（州）级兽医实验室开展了检测能力比对。结果显示：2018—2020年全省整体平均正确率分别为95.59%、97.09%和97.88%，说明四川省地市（州）级兽医实验室检测能力处于较高水平，能满足非洲猪瘟、禽流感等重大动物疫病检测需要。同时，部分实验室也反映出一些问题，如设备差、检测水平不高、检测报告不规范等。建议加大兽医实验室建设力度，更新完善检测设施设备；加强实验室技术人员培训，提升其检测能力和水平；同时继续推进兽医系统实验室考核工作，加强比对考核。     

非洲猪瘟病毒核酸定性检测方法比对

为提升兽医实验室非洲猪瘟病毒核酸检测能力和质量控制水平,积累检测经验,以更好地开展非洲猪瘟检测,2020年7月,参加了北京市农业农村局组织的“非洲猪瘟实验室检测能力比对”项目。本次比对同时选用《非洲猪瘟检疫技术规范》(SNT 1559—2010标准)中的普通PCR方法、荧光定量PCR方法以及商品化试剂盒,对5份验证样品开展非洲猪瘟病毒核酸检测和结果比对。比对发现,3种检测方法结果一致,样品检测结果与预期一致,结果满意。此次比对确认了实验室现有检测方法能够满足非洲猪瘟病毒核酸的日常检测需求,同时提升了实验室人员的检测能力,积累了检测经验,可为非洲猪瘟防控提供有力的技术支撑。     

Heartworm in dogs in Canada in 1984

In late December 1984, 1853 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1984 and 35% of them responded. Veterinarians reported that 97,794 dogs were blood-tested to check for microfilariae and 1417 dogs (1.45% of those tested) were found with heartworm. Another 34 dogs were amicrofilaremic, but were diagnosed as having heartworm disease, to give the total number diagnosed in 1984 as 1451 (1.48%). Heartworm was reported from all provinces except Prince Edward Island and Newfoundland but most (1310) of the cases were in Ontario. In Quebec, 126 cases were reported mostly from west of Montreal.

Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 27% of the cases were observed with clinical signs of heartworm disease and 72% had a history of not having left Canada. Southwestern Ontario continued to be the primary focus of the infection.

     

Heartworm in dogs in Canada in 1988

In late November 1988, 1581 small and mixed animal clinics and institutional veterinarians across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1988, and 46% of them responded. Veterinarians reported that 181,577 dogs were blood-tested for heartworm disease and 367 dogs were found with D. immitis microfilariae. Another 60 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number of cases diagnosed in 1988 as 441 (0.24%).     

Heartworm in dogs in Canada in 1985

In late December 1985, 1485 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1985 and 44% of them responded. Veterinarians reported that 137,300 dogs were blood-tested to check for microfilariae and 1210 dogs were found with heartworm. Another 36 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number diagnosed in 1985 as 1247 (0.91%).

Heartworm was reported from all provinces except Prince Edward Island, Newfoundland and Saskatchewan but most (1126) of the cases were in Ontario. Southwestern Ontario continued to be the primary focus of the infection in Canada. From Quebec, 91 cases were reported mostly from and around Montreal. From Manitoba, 19 cases were reported from Winnipeg and surrounding areas. Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 28% of the cases were observed with clinical signs of heartworm disease and 78% had a history of not having left Canada.

     

Heartworm in dogs in Canada in 1989

In late November 1989, 1732 clinics and institutional veterinarians were sent a questionnaire to assess the status of Dirofilaria immitis, and 51.7% responded. Of 247,716 dogs tested, 394 had D. immitis microfilariae and 51 were amicrofilaremic for a total of 445 cases and heartworm prevalence of 0.17%. Most (408) of these dogs had no preventive medication and the prevalence among dogs tested and unprotected was 1.01%. That prevalence was considerably higher in endemic areas. Thirty-seven dogs with heartworm had preventive medication. Heartworm was most frequent in companion dogs over three years of age maintained outdoors in rural areas. About 75% of the cases had never left Canada, 26% had clinical signs and 125 were not treated.

Heartworm was reported from British Columbia, Manitoba, Ontario, Quebec, Nova Scotia and Newfoundland, but 383 cases were in Ontario. South-western Ontario was the primary focus of infection. There were 33 cases in Quebec and 24 in Manitoba, mainly found in and around Metropolitan Montreal and Winnipeg respectively.

     

Heartworm in dogs in Canada in 1986

In late December 1986, 1224 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1986; 46% of them responded. Veterinarians reported that 150,989 dogs were blood-tested for microfilariae and 869 dogs were found with heartworm. Another 65 dogs were amicrofilaremic but diagnosed with heartworm disease and one was found with heartworm at necropsy to give the total number diagnosed in 1986 as 935 (0.62%).

Heartworm was reported from Manitoba, New Brunswick, Ontario and Quebec, but most (810) of the cases were from Ontario. South-western Ontario continued to be the primary focus of the infection in Canada. There were 103 cases reported from Quebec, mostly from and around Montreal, and 21 cases from Manitoba, from Winnipeg and surrounding areas. Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 33% of the cases were observed with clinical signs of heartworm disease and 81% had a history of not having left Canada.

     

Heartworm in dogs in Canada in 1987

In late November 1987, 1246 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1987, and 50% of them responded. Veterinarians reported that 165,428 dogs were blood tested for heartworm disease and 511 dogs were found with D. immitis microfilariae. Another 78 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number of cases diagnosed in 1987 as 589 (0.35%).     

Heartworm in dogs in Canada in 1983

In late December 1983, 2 800 veterinarians across Canada were sent a questionnaire in order to assess the status of heartworm disease in Canada in 1983 and 26% of them responded. Veterinarians reported that 59 504 dogs were blood-tested to check for microfilariae and 771 dogs (1.30% of those tested) were found with Dirofilaria immitis. Heartworm disease was diagnosed in all provinces except New Brunswick and Newfoundland but most (733) of the cases were in Ontario.

Heartworm disease was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 31% of the cases were observed with clinical signs of heartworm disease and 64% had a history of not having left Canada. Southwestern Ontario continues to be the focus of the infection and most of the dogs there had not left the province previously.