应用氧化酶检验肉品

我们采用氧化酶法对肉品的新鲜度进行了检验，并与氢离子浓度测定法、过氧化物酶反应法、硫酸酮肉浸液反应等方法进行对比，取得了满意效果。１材料及方法１．１试剂０．２％联苯胺酒精液；１０％过氧化氢液。１．２被检肉样由市场购新鲜猪肉样８份、鲜牛肉样６份，在室温...     

病死畜禽肉的三种检验方法

运用硫酸铜沉淀反应原理、过氧化物酶反应原理、细菌毒素氧化呈色反应原理对随机抽取的牛肉、猪肉、羊肉共５００份肉样进行检验 ,检出阳性肉样５２份 ,占１０．４ % ,都属病死畜禽肉和劣质肉。综合判定阳性检出率高 ,检验结果准确。目前 ,对上市畜禽肉的检疫（验）多凭感官检查 ,方法简单 ,科技含量不高 ,对一些隐性感染和无临床症状的染疫肉品很难检出 ,肉品检出合格率低下 ,很难保证人民群众真正吃上“放心肉”。为此 ,我们建立了《综合检验病、死畜禽肉的研究》课题 ,采用“硫酸铜沉淀、过氧化物酶、细菌毒素的氧化呈色反应”综合检验方…     

肉品新鲜度理化检验方法的比较

为了快速、准确地对市场肉品的新鲜度进行检验,我们对微量扩散法、球蛋白沉淀法、测定pH,纳勒氏试剂氨反应对肉品新鲜度检验的灵敏度进行了试验比较,现将结果介绍如下:一、材料与方法1.肉样经市场检疫员感官检查判为疑似变质的肉样150份,每份采取不含肌腱、脂肪的肌肉50g待检.2.试剂注射用生理盐水及无氨蒸馏水;2%硼酸、混合指示剂、饱和碳酸钾溶液、0.01N盐酸溶液、水溶性胶;pH 5-8的试纸或25型酸度计;10%硫酸铜溶液;新配制的纳勒氏试     

检查肉品鲜度的试验——过氧化物酶反应直接滴肉法

在农贸市场,对肉品进行兽医卫生检验时,多以感观为主,有时辅以必要的实验室检验。但实验室检验因受时间、药械、场地等条件的限制,在农贸市场检验时较少应用。为了寻求快速、简便、准确,适合于农贸市场应用的检验手段,我们以直接滴肉法代替肉浸液进行常规的过氧化物酶反应法,取得了良好的试验结果,现将具体情况报告如     

一起染疫、病死动物产品的鉴定

在实际工作中 ,染疫、病死的动物产品多已被不法经营者分割 ,其胴体完整性遭到破坏 ,且往往不带皮肤 ,脱离脏器 ,因而从感官上不易得出全面准确的结论 ,给依法办案带来被动。笔者曾对一起疑似染疫、病死动物产品进行理化检验 ,鉴定为染疫、病死动物产品。１材料与方法１ １材料待检肉为河南省某县兽医卫生监督所查获的该县一个体冷库贮存的分割猪肉。对照用健康新鲜肉购自集贸市场 ,盖有全国统一动物检疫合格验讫印章 ,且经感官检疫为正常。１ ２感官检疫按常规方法进行检疫。１ ３过氧化物酶反应取１∶１０肉浸液２ｍｌ,置于试管中后滴加４…     

球蛋白沉淀试验快速检验肉质新鲜度

感官检查肉质新鲜度，其结果往往难以令人信服。经］临床应用，找们证明球蛋白沉淀试验是一种简便、快速检验肉质新鲜度的可靠方法，从而解决了这一难题。现将操作介绍如下：称取肉检样5克，剪碎放入三角瓶中，加蒸馏水25毫升，浸泡并不断探病．25分钟后过滤，留滤液备用。再备2支试管，一支加肉浸液2毫升，另一只加蒸馏水2毫升作对照。给两试管各加5滴10％硫酸铜溶液，充分摇晃后观察。对照试管内液体呈淡蓝色透明状；新鲜肉也呈淡蓝色透明状；次鲜肉轻度浑浊；变质肉则浑浊共有白色沉淀。球蛋白沉淀试验快速检验肉质新鲜度@崔守龙$滁县地…     

对肉新鲜度的几种简易理化检验方法的评价

目前,我国规定用挥发性盐基氮作为肉新鲜度的理化指标。由于在测定此项指标时,有相对费时,麻烦等缺点。所以在实际工作中,一些基层单位经常采用感官检验结合其它几项简易理化检验方法,如肉pH值测定、粗氨测定、硫酸铜反应、过氧化物酶     

掺亚硝酸盐牛乳快速检验方法研究

应用四种试剂和一种试纸,对掺亚硝酸盐牛乳的检验方法进行了研究。结果表明,由甲萘胺、对氨基苯磺酸、酒石酸制成的检验粉剂具有简便、快速等特点,最低检出量为0.2ppm,适合于现场快速检验。     

掺甲醛牛乳快速检测试纸的研制

为解决在牛乳收购环节快速检测出掺甲醛牛乳的方法,采用试管法和试纸法对牛乳掺甲醛进行检测比较,确定了试纸检测牛乳中掺甲醛的方法可行.探索了采用品红试剂制作的试纸方法,筛选出品红溶液和硫酸溶液最佳浓度,两种溶液按3:1比例加载到WFB-试纸上,自然干燥.用掺有不同浓度甲醛的牛乳测试试纸的灵敏度,检测结果表明,最低检出量为0...     

濮阳市出台病死畜禽肉鉴别技术规范

病死畜禽肉的监督监管是动物防疫工作的重要组成部分，11月1日，濮阳市《病死畜禽肉鉴别技术规》（DB4109／T042—2007）正式实施。该规范在全省尚属首创。《规范》明确了我市境内从事动物检疫监督、监测和技术仲裁中病死畜禽肉鉴别及处理等相关活动时鉴别病死畜禽肉的感官特征、生物化学检验和处理方法，并附有《病死畜禽肉生物化学检验方法》，[第一段]     

癸氧喹酯干混悬剂含量测定的改进

采用高效液相色谱法测定癸氧喹酯干混悬剂的含量,在2-250μg/mL范围内,峰面积的常用对数与进样量浓度的常用对数呈良好的线性关系,R^2=1(n=5),平均回收率为99.24%~99.51%,RSD在0.05%~0.28%。此方法分析时间短,样品前处理简便、定量结果准确,重现性好,结果满意,为其质量控制提供了依据。     

猪毛色遗传的研究进展

本文概述了猪的毛色类型、猪的毛色遗传模式,着重综述了猪毛色基因分子基础的研究进展,指出存在问题并就未来发展方向做了思考。     

商会自治的基石——商会自治规范研究

在现代法律秩序中,商会自治规范是制定法的基础和必要的补充,甚至在某些方面替代了制定法;商会自治规范主要包括商会组织规范、行为规范、惩罚规范以及争端解决规范等;其效力仅及于其内部成员;商会自治规范和制定法之间存在冲突,但也存在整合的基础。     

Effects of size of ingestively masticated fragments of plant tissues on kinetics of digestion of NDF

Ingestively masticated fragments were collected and sized via sieving. Different sizes of esophageal masticate and ruminal digesta fragments, and ground fragments of larger masticated pieces were incubated in vitro, and undigested NDF remaining at intervals of up to 168 h of incubation was determined. The ruminal age-dependent time delay (tau) for onset of digestion of NDF was positively correlated (P < 0.004) with the mean sieve aperture estimated to retain 50% of the fragments between successive sieve apertures (MRA). Degradation rate of potentially degradable NDF (PDF) and level of indigestible NDF were not related (P > 0.10) to MRA of masticated and ground fragments. Estimates of tau were positively related to MRA, with slopes of bermudagrass < corn silage < ruminal fragments of corn silage. It was concluded that fragment size-, and consequently, ruminal age-dependent onset of PDF degradation of a mixture of different fragment sizes results in an age-dependent rate of degradation of the more rapidly degrading of two subentities of PDF. Models are proposed that assume a tau before onset of simultaneous degradation of PDF from two pools characterized as having gamma-modeled age-dependency and age-constant rates. The ruminal age-dependent pool seems to be associated with the faster-degrading pool, and its rate parameter increases with range in MRA in the population of fragments. Conceptually, the ruminal age-dependent rate parameter for PDF degradation seems to represent a composite of several effects: 1) effects of the size-dependent tau; 2) range in MRA of the population of ingestively masticated fragments; and 3) subentities of PDF that degrade via more rapid age-dependent rates compared with subentities of PDF that degrade via age-constant rates. The estimated fractional rates of ruminative comminution of ingestively masticated fragments (0.060 to 0.075/h) were of a magnitude similar to the mean fractional rates of PDF digestion (0.030 to 0.085/h), which implies that ruminative comminution may be first-limiting to fractional rate of PDF digestion. The in vivo roles of ingestive and ruminative mastication of fragments on PDF degradation must be considered in any kinetic system for estimating PDF digestion in the rumen. These results and others in the literature suggest that the rate of surface area exposure rather than intrinsic chemical attributes of PDF may be first-limiting to degradation rate of PDF in vivo.     

中华人民共和国农业部公告 第267号

为贯彻落实《兽药生产质量管理规范》(简称《兽药GMP》),进一步推动兽药GMP实施进程,我部制定了《兽药生产质量管理规范检查验收办法》,现予公告。本公告自2003年6月1日起施行。附件:兽药生产质量管理规范检查验收办法二○○三年四月十日第一章 总则 第一条 为推动《兽药生产质量管理规范》(以下简称兽药GMP)的实施,规范兽药GMP检查验收工作,制定本办法。 第二条 农业部负责全国兽药GMP管理和检查验收工作;负责制修订兽药GMP检查验收管理规定;负责兽药GMP检查员队伍建设和监督管理工作,负责国际兽药贸易中GMP互认工作。 …     

鸡败血支原体垂直传播模式及其阻断方法

以国际标准强毒Ｒ株人工感染非免疫产蛋鸡,定时扑杀,分别从鼻窦、眶下孔、气管、肺、气囊、卵巢和输卵管分离ＭＧ,并收集感染鸡所产蛋分离ＭＧ。结果表明,人工感染４８小时后上、下呼吸道及肺已被全面感染,９６小时气囊已被感染,１２０小时输卵管已能分离到ＭＧ,卵巢始终分离不到ＭＧ。人工感染鸡自１４４小时便能在其所产蛋中分离出ＭＧ。药物治疗能在７２小时内消除感染,油乳剂苗则需２４天后逐渐降低蛋内ＭＧ分离率,药物卵内注射、种蛋药浴、高温处理均能杀死卵内ＭＧ,但以研制的种蛋浸泡剂药浴效果为最好。     

Mechanism of Action of Probiotic Function of Lactobacilli

乳酸杆菌作为益生菌广泛用于人和动物.本文综述了乳酸杆菌改善宿主健康的机制.乳酸杆菌可通过产生抗菌物质如乳酸、过氧化氢、细菌素,或者通过竞争营养或肠道黏附位点来抑制致病菌;通过诱导黏附素的分泌或阻止细胞凋亡而增强肠道的屏障功能,从而保护肠道.文章重点讨论了乳酸杆菌表面成分(表面蛋白、脂磷壁酸和肽聚糖)与肠道受体(C型凝集素受体、Toll样受体和Nod样受体),阐述了他们结合后启动免疫调节信号,调控肠道免疫功能以发挥改善健康作用的机制.     

獭兔睾丸的组织学观察

家兔作为一种实验动物 ,推动了繁殖技术的发展。本试验通过对不同年龄公獭兔的睾丸进行组织学观察、测定 ,研究精子的发生规律 ,为系统地进行繁殖生理工作提供依据。1　材料与方法选 60日龄、75日龄、90日龄 3个年龄公獭兔各5只 ,用外科手术法摘取两侧睾丸 ,放入 Bouin氏液中固定 ,二甲苯透明 ,石蜡包埋 ,切成 5～ 8μm切片 ,H.E.染色。在显微镜下观察 ,并进行定量组织学指标测定及差异性比较。2　结果和讨论2 .1　睾丸定量组织学指标的测定结果　见表 1。表 1　獭兔睾丸定量组织学指标　　　μm,个 /精细管60日龄 75日龄 90日龄曲细精管…     

Comparison of 2 methods of centesis of the bursa of the biceps brachii tendon of horses

REASONS FOR PERFORMING STUDY: Centesis of the bicipital bursa using an 8.9 cm long spinal needle has been reported but the alternative of employing a 3.8 cm long hypodermic needle requires validation. OBJECTIVE: To compare the efficacy of 2 different methods of centesis of the bicipital bursa and to evaluate the usefulness of ultrasonographic imaging to determine the location of solution administered when centesis of the bursa is attempted. METHODS: For Trial 1, 6 clinicians, who had no previous experience of centesis of the bicipital bursa, attempted to inject a solution composed of an aqueous radiopaque contrast medium and physiological saline solution (PSS) into the bicipital bursae of 2/12 horses using the previously described distal approach to inject one bursa and a proximal approach to inject the contralateral bursa. The bicipital tendon and bursa were examined ultrasonographically before and after injection; and both shoulders were examined radiographically to identify the location of the medium. In Trial 2, another 6 clinicians, also with no previous experience of centesis, repeated Trial 1, using 6 horses, but the radiopaque contrast medium was mixed with air instead of PSS. RESULTS: Accuracy of centesis using the proximal approach was 39% and that of the distal approach 28%. Ultrasonographic examination of the shoulder allowed the location of solution and air to be accurately predicted in all 12 shoulders examined. CONCLUSIONS: Clinicians who have had no previous experience performing centesis of the bicipital bursa are unlikely to be successful in centesis using either approach. Radiographic examination after injecting a radiopaque contrast medium may be necessary to assess the success of centesis especially if bursal fluid is not obtained during centesis. Injecting air along with the radiopaque contrast medium provides more accurate ultrasonographic confirmation of centesis and better radiographic definition than does injection without air.