Effects of feeding embrittlement feed on growth and flesh quality of

为考察脆化饲料对罗非鱼(Tilapia)生长和肌肉品质的影响,实验分别以配合饲料(对照组)、脆化饲料(试验组)喂养体质量为(115.30±23.69)g的罗非鱼99d.结果显示:配合饲料组和脆化饲料组罗非鱼的增重率分别为185.25％、94.96％;饲料系数为1.87、2.94;肥满度为15.23、12.50;肌肉粗蛋白含量为19.40％、18.26％;粗脂肪含量为0.75％、1.32％;胶原蛋白为2.03、2.91;肌原纤维耐折力为33.93 μm、64.84 μm;滴水损失为2.59％、1.82％.与对照组相比,在生长方面,脆化饲料组罗非鱼增重率、肥满度显著降低,饲料系数显著增加(P＜0.05);在肌肉品质方面,肌肉的粗蛋白含量和滴水损失显著降低,而粗脂肪含量、胶原蛋白含量和肌原纤维耐折力显著提高(P＜0.05).结果表明,脆化饲料在一定程度上改善了罗非鱼的肌肉品质.     

投饲蚕豆对草鱼生长和肉质影响的初步研究

分别以配合饲料(对照组)、蚕豆(蚕豆组)饲喂平均体重为496.5 g的草鱼(Ctenopharyngodon idella)66d,考察蚕豆对草鱼生长和肉质的影响。结果表明:配合饲料组和蚕豆组增重率为82.76%,36.96%;饵料系数为2.17,4.45;肥满度为13.09,7.72;粗脂肪含量为0.89%,1.43%;肌肉失水率为27.14%,15.87%;肌原纤维长度为28.32μm,33.31μm;羟脯氨酸0.12mg/g,0.16mg/g。在生长方面,与配合饲料组相比,蚕豆组草鱼的增重率、肥满度极显著降低,饲料系数极显著增加(P<0.01);在肌肉品质方面,失水率极显著降低;粗脂肪含量、肌原纤维长度、羟脯氨酸含量极显著提高(P<0.01)。结果表明,投喂蚕豆对草鱼肌肉具有特殊的改变作用。     

桑叶黄酮对吉富罗非鱼肌肉抗氧化指标及营养组成的影响

为研究饲料中添加桑叶黄酮对吉富罗非鱼(Oreochromis niloticus)肌肉抗氧化指标及营养组成的影响,选取初始体重为(1.51±0.02)g的吉富罗非鱼840尾,随机分为6组,每组4个重复,每个重复35尾鱼,在56 d饲养期中分别投喂添加桑叶黄酮0、50、100、300、500和1 000 mg/kg的试验饲料。结果显示:100、300 mg/kg组肌肉超氧化物歧化酶(SOD)活性显著高于其他各组,且分别比对照组提高了6.2%和8.7%;500 mg/kg组肌肉总抗氧化力(T-AOC)显著高于1 000 mg/kg组及对照组;50、100、300、1 000 mg/kg组肌肉丙二醛(MDA)含量显著低于对照组。饲料中添加桑叶黄酮对吉富罗非鱼肌肉粗蛋白、粗脂肪、灰分和水分含量没有显著影响,对肌肉滴水损失影响不显著。500 mg/kg组胶原蛋白含量显著高于对照组,与其他组差异不显著。50 mg/kg组肌肉苯丙氨酸含量与对照组相比显著升高。100 mg/kg组肌肉丙氨酸含量显著高于对照组和50、300、500 mg/kg组。300 mg/kg组肌肉亮氨酸、蛋氨酸和谷氨酸和1 000 mg/kg组肌肉组氨酸含量显著高于对照组。结果表明,添加适量的桑叶黄酮可以显著提高吉富罗非鱼肌肉抗氧化指标和胶原蛋白含量,改善肌肉氨基酸组成。综合肌肉抗氧化性能、胶原蛋白含量和氨基酸组成的指标来评价,桑叶黄酮添加水平在300~500 mg/kg时效果较好。     

吉富罗非鱼对饲料中苯丙氨酸的需要量

分别用含6种水平(质量比分别为0.78%、0.95%、1.09%、1.34%、1.51%和1.72%)苯丙氨酸(phenylalanine,Phe)的等氮等能(粗蛋白30.10%,总能17.73 MJ/kg)饲料,在池塘网箱中(实验期间水温为24~32℃)饲喂初始体重为(52.70±1.80)g的吉富罗非鱼60 d,考察饲料Phe对吉富罗非鱼(GIFT,Oreochromis niloticus)生长性能、饲料系数、体成分、部分血清生化指标及前肠消化酶活性的影响,以期获得吉富罗非鱼对饲料苯丙氨酸的需要量。结果表明,随着饲料中Phe水平的升高,吉富罗非鱼的增重率、特定生长率、蛋白质效率、蛋白质沉积率、肥满度、肝体比和脏体比均呈现先上升后下降的趋势,饲料系数呈现先下降后上升的趋势;全鱼粗脂肪和全鱼灰分含量显著上升(P0.05),肌肉灰分含量显著下降(P0.05)。饲料中Phe对全鱼水分和粗蛋白质、肌肉水分、肌肉粗蛋白质和肌肉粗脂肪含量无显著性影响(P0.05);各实验组的肌肉氨基酸含量差异不显著(P0.05)。饲料中Phe显著影响血清中谷丙转氨酶活性,甘油三酯、总胆固醇和葡萄糖的含量(P0.05),对谷草转氨酶活性无显著性影响(P0.05);Phe显著影响肠蛋白酶、肠脂肪酶和肠Na~+-K~+-ATP酶活性(P0.05),而对肠淀粉酶活性影响不显著(P0.05)。以增重率、饲料系数和蛋白质效率为评价指标,通过二次回归分析可知,吉富罗非鱼对饲料中Phe需要量为1.17%~1.21%,占饲料蛋白质的3.89%~4.02%。本研究结果为合理配制吉富罗非鱼配合饲料提供了理论依据。     

不同饲料原料对草鱼生长和肌肉品质的影响

分别以专用配合饲料、浸泡蚕豆、浸泡豌豆喂养体质量为(1540.9±57.5)g的草鱼(Cteno-pharyngodon idellus)90d,以考察不同原料对草鱼生长和肌肉品质的影响。结果表明,配合饲料组、浸泡蚕豆组和浸泡豌豆组草鱼的增重率分别为17.3%、13.7%和12.2%,饵料系数分别为5.00、4.09和5.08,三种原料对草鱼的脏体比、肝体比没有显著影响;与普通草鱼相比,三种原料组草鱼肌肉粗脂肪含量和肌肉失水率显著降低(P0.05),胶原蛋白含量和肌原纤维长度显著增加(P0.05)。上述结果表明,三种原料均可显著改善草鱼肌肉品质;与浸泡蚕豆组相比,专用配合饲料组显著提高了草鱼的生长性能。     

蝇蛆粉替代鱼粉对罗非鱼生长、肌肉组成和肝脏非特异性免疫指标的影响

为探讨以蝇蛆粉替代鱼粉对吉富罗非鱼生长性能、肌肉组成和肝脏非特异性免疫指标的影响,将450尾初始体质量为(80.26±7.36)g的吉富罗非鱼随机分为6组,每组3个平行,进行了饲养对比试验。试验组分别用蝇蛆粉替代基础饲料中20 %、40 %、60 %、80 %和100 %的鱼粉,对照组投喂基础饲料,养殖周期60 d。试验结果,以蝇蛆粉替代鱼粉,各替代水平组试验鱼的终末体质量、增重率、特定生长率、肥满度、成活率、饲料系数、肌肉中粗蛋白、水分含量、肝脏丙二醛含量无显著差异(P＞0.05)。除20 %替代组外,其它各试验组鱼的肝体比、脏体比与对照组相比显著降低(P＜0.05)。随着蝇蛆粉替代水平的增加,罗非鱼肌肉中粗灰分、总磷的含量显著降低(P＜0.05)。替代水平不超过40 %时,各替代组试验鱼肌肉中粗脂肪、钙及肝脏超氧化歧化酶的含量无显著差异(P＞0.05);当替代水平不小于60 %时,试验鱼肌肉中粗脂肪、钙的含量显著降低(P＜0.05),但肝脏超氧化歧化酶含量显著升高(P＜0.05)。结果表明,在本试验条件下,以生长性能、肌肉成分和肝脏非特异性免疫作为评价指标,蝇蛆粉可完全替代罗非鱼饲料中的鱼粉。     

盐度对草鱼生长和肌肉品质的影响

为考察盐度对草鱼生长和肌肉品质的影响，设置盐度为0(对照组)，5.0、7.5、10.0的4个水平，饲养平均体重为(725±42) g的草鱼30 d，测定草鱼生长性能、肌肉营养成分和肌肉物理性能指标。结果表明，在0、5.0、7.5盐度中草鱼生长良好，成活率均为100%,增重率为27.6%、28.3%、26.4%，但在盐度为10.0的水体中，草鱼成活率和增重率均极显著降低（P<0.01），仅为60%、4.5%, 而饲料系数显著上升（P<0.01）；在肌肉基本营养成分方面，盐度为0，5.0，7.5各组草鱼肌肉水分、粗蛋白、灰分含量无显著差异，但盐度为10的草鱼组肌肉水分含量显著增加，粗蛋白含量降低。经过不同盐度水体暂养后，草鱼肌肉中脂肪含量显著减少（P<0.01），胶原蛋白含量增加，肌纤维耐折力提高，肌纤维直径减小，而盐度对肌肉失水率的影响则呈现不同的特点，其中5.0、10.0盐度使肌肉失水率显著上升（P<0.01），而7.5盐度则使肌肉失水率显著下降（P<0.01）。上述结果表明，半咸水暂养草鱼可改善其肌肉品质，适宜的盐度值为7.5。表4参28     

黑水虻幼虫培养基替代豆粕对吉富罗非鱼生长、体组成和血清生化指标的影响

在实用饲料(含豆粕35.0%,豆粕粗蛋白质含量为47.7%)的基础上,用黑水虻幼虫培养基分别替代0(G0)、15%(G15)、30%(G30)、45%(G45)的豆粕,配制成4种等氮(31%),等脂(6%)的实验饲料,以研究罗非鱼(Oreochromis niloticus)饲料中黑水虻幼虫培养基替代豆粕的可行性。将400尾初始体重为(2.30±0.02)g的罗非鱼随机分成4组(每组设4个重复,每个重复25尾),其中以饲喂G0实验饲料的组为对照组,实验期为8周。结果显示:黑水虻幼虫培养基替代豆粕对罗非鱼增重率、饵料系数、存活率影响不显著。与G0组相比,各替代组罗非鱼全鱼粗蛋白、灰分、水分含量差异不显著,但随着替代量的增加,全鱼粗脂肪含量显著降低,G45组全鱼粗脂肪含量显著低于对照组。黑水虻幼虫培养基替代豆粕对罗非鱼肥满度影响不显著,但肝体比随着替代量的增加显著降低,在30%的替代量时差异达到显著水平。与G0组相比,各替代组罗非鱼血清白蛋白、球蛋白、血糖、甘油三酯、胆固醇、谷丙转氨酶、谷草转氨酶以及尿素含量差异不显著。由以上研究结果得出,在豆粕用量为35%的基础饲料中,黑水虻幼虫培养基可替代豆粕用量的45%而不影响罗非鱼生长,黑水虻幼虫培养基在基础饲料中的添加量为34.6%,占饲料总蛋白质含量的23.69%;黑水虻幼虫培养基可显著降低罗非鱼全鱼粗脂肪含量以及肝体比。     

饲料组成对中国对虾肌肉组织中胶原蛋白、肌原纤维和失水率的影响↑（*）

用不同饲料喂养中国对虾,结果表明,饲料成分是影响肌肉组织中胶原蛋白含量、肌原纤维耐折断力和失水率大小的重要因素。鲜活饲料和维生素Ｃ能促进胶原蛋白的形成,加强对虾运动,也可提高肌肉组织中胶原蛋白的含量,野生虾肌肉胶原蛋白的含量大于养殖虾。饲料成分对胶原蛋白含量的影响规律和对肌原纤维耐折断力的影响相同,而对失水率的影响相反。肌原纤维耐折断力和失水率与胶原蛋白含量分别是正相关和负相关关系,其回归直线分别为＾Ｙ＝－５６７＋３０６６Ｘ和＾Ｙ＝６７３６－１５９６８Ｘ。上述３者影响肌肉组织柔韧性,即胶原蛋白含量越高,肌原纤维耐折断力越好,失水率越小,肌肉组织的柔韧性越强。     

饲料牛磺酸对尼罗罗非鱼生长、体成分及组织游离氨基酸含量的影响

本文探讨了饲料牛磺酸水平对尼罗罗非鱼幼鱼生长性能、体成分及游离氨基酸含量的影响。选用体重为5.89±0.03g的罗非鱼300尾,随机分为5个处理组,每个处理3个重复,每个重复20尾。在基础饲料中分别添加0、0.4%、0.8%、1.2%、1.6%的牛磺酸,配制成5种试验料,分别饲喂不同处理组的罗非鱼,饲养周期为56d。结果表明： 0.8%牛磺酸组罗非鱼增重率最高,且显著高于0%和1.6%组(P<0.05);饲料牛磺酸水平(0.4%-1.2%)显著提高了罗非鱼摄食率、肝脏指数和内脏指数,显著降低了饲料系数(P<0.05)。随着饲料牛磺酸水平的提高,鱼体粗蛋白质和粗脂肪含量随之升高,鱼体水分和粗灰分含量随之降低(P<0.05);以增重率为指标,通过二次曲线回归分析得出罗非鱼饲料牛磺酸最适需要量为0.75%。罗非鱼血清、肝脏、肌肉和全鱼中牛磺酸含量与饲料牛磺酸含量存在正相关关系,且牛磺酸添加组血清、肝脏、肌肉和全鱼牛磺酸含量显著高于对照组相(P<0.05)。罗非鱼肝脏和肌肉中游离氨基酸含量随饲料牛磺酸含量的增加均呈逐渐下降的趋势,且牛磺酸添加组肝脏和肌肉中游离氨基酸含量显著低于对照组(P<0.05)。     

Effects of Dietary Geniposide on Growth,Flesh Quality,and Lipid Metabolism of Grass Carp,Ctenopharyngodon idella

The aim of this study was to investigate effects of dietary geniposide (GP) on growth performance, flesh quality, and lipid metabolism of grass carp, Ctenopharyngodon idella (95.2 ± 0.6 g), fed seven different diets, including a control diet; Eucommia ulmoides (EU)–supplemented diet (20 g/kg); and GP‐supplemented diets containing 100, 200, 400, 600, and 800 mg/kg GP, respectively. Weight gain rate was significantly improved (P < 0.05) and feed conversation ratio was significantly decreased (P < 0.05) by supplementation of EU. Grass carp fed 100–800 mg/kg GP‐supplemented diets showed significantly higher total collagen and alkaline‐insoluble collagen content in muscle than control (P < 0.05). Contents of total collagen and the alkaline‐insoluble collagen content in the skin of grass carp were significantly increased by dietary 600–800 mg/kg GP and EU (P < 0.05). Fish fed diets containing 600–800 mg/kg GP showed significantly lower muscle crude lipid content than the EU, control, and 100–400 mg/kg GP groups (P < 0.05). Fish fed 400–800 mg/kg GP diets had significantly higher muscle fiber density and lower muscle fiber diameter and serum triglyceride level than the control (P < 0.05). In conclusion, supplementation of GP could improve flesh quality, but not growth of grass carp. The supplemental level of GP for improving flesh quality was estimated to be a 400–600 mg/kg diet.     

投喂蚕豆对草鱼生长及肌肉营养特性分析

分别用配合饲料、浸泡蚕豆和发芽蚕豆喂养平均体重(1000±20)g草鱼70d,分析投喂蚕豆对草鱼生长及肌肉营养的影响。结果表明,配合饲料组、浸泡蚕豆组和发芽蚕豆组的草鱼增重率分别为79.5%、40.32%和50.10%,饵料系数分别为2.11、4.78和4.31,投喂蚕豆降低了草鱼增重率(P<0.05),显著增加了饵料系数(P<0.05)。与配合饲料组相比,浸泡蚕豆组和发芽蚕豆组肌肉中粗脂肪和粗蛋白含量显著降低(P<0.05)。浸泡蚕豆组和发芽蚕豆组的必需氨基酸含量、鲜味氨基酸含量、氨基酸总量比配合饲料组显著提高(P<0.05)。投喂蚕豆降低了草鱼的生长性能,但是显著提高了草鱼肌肉品质。投喂发芽蚕豆的效果要优于投喂浸泡蚕豆。     

The influence of a dietary lipid source on growth, muscle fatty acid composition and erythrocyte osmotic fragility of hybrid tilapia

An 8-week feeding trial was conducted to determine the effects of various dietary lipids on the growth, tissue proximate composition, muscle fatty acid composition and erythrocyte osmotic fragility of red hybrid tilapia, Oreochromis sp. Five isonitrogenous and isoenergetic semipurified diets were supplemented with 10% of either cod liver oil (CLO), sunflower oil (SFO), crude palm oil (CPO), crude palm kernel oil (CPKO), or a combination of 5% CLO with 5% palm fatty acid distillates (PFAD), respectively. There were no significant effects (P > 0.05) of diet on growth but fish fed the CLO diet showed a significantly (P< 0.05) poorer feed efficiency ratio compared to fish fed the CPO diet. Lipid deposition in fish muscle was mostly similar among fish fed the various diets but bone ash was significantly higher in fish fed the CPO and CPKO diets. Muscle lipids of fish fed palm oil-based diets did not increase in saturated fatty acids content but showed significantly lower polyunsaturated fatty acid (PUFA) concentrations compared to fish fed the CLO diet. The concentrations of individual PUFA in muscle lipids were strongly influenced by dietary PUFA concentrations. Dietary lipids did not markedly affect the structural integrity of erythrocyte membranes but the erythrocytes of tilapia fed the CPO diet were slightly more resistant to osmotic lysis. It was concluded that palm oil products, especially CPO, could be successfully used in the diet of hybrid tilapia based on its availability, cheaper costs and its potential ability to enhance oxidative stability due to its low PUFA content and high natural concentrations of antioxidants.     

投喂频率对绿鳍马面鲀幼鱼生长和肌肉品质的影响

为研究不同投喂频率对绿鳍马面鲀幼鱼生长性能和肌肉品质的影响,在室内循环水养殖系统中,采用人工配合饲料,以1次/d、2次/d、3次/d、4次/d等4种投喂频率,对体质量(13.8±0.83)g的绿鳍马面鲀幼鱼进行了饲养试验,试验时长为42 d。结果显示,随着投喂频率增加,绿鳍马面鲀幼鱼的终末体质量呈逐渐升高的趋势,特定生长率呈先升后降的变化趋势,其中3次/d组幼鱼的特定生长率最高,显著高于1次/d组(P<0.05);饲料系数也随着投喂频率的增加而升高,3次/d组的饲料系数显著高于1次/d组(P<0.05),4次/d组的饲料系数显著高于1次/d组和2次/d组(P<0.05);肌肉品质方面,4次/d组幼鱼的肌肉粗脂肪含量显著高于1次/d组(P<0.05),不同投喂频率组间肌肉粗蛋白质含量和灰分含量无显著性差异(P<0.05)。综合考虑生长性能和肌肉品质指标,绿鳍马面鲀幼鱼适宜的投喂频率为日投3次。     

Performance of genetically improved Nile tilapia compared with red hybrid tilapia fed diets containing two protein levels

A 10‐week feeding trial was conducted to investigate the interaction between tilapia genotype and dietary protein level on the growth, feed utilization efficiency and body composition of Nile tilapia [Oreochromis niloticus L., F₉ generation of genetically improved farmed tilapia (GIFT) strain] and red hybrid tilapia (Oreochromis sp.). Two isoenergetic diets (15.5 kJ g⁻¹ diet) containing 25% or 35% crude protein were formulated and fed to triplicate groups of tilapia (12.8±0.2 g) at a fixed daily ration of 4% of their body weight. The growth performance of the GIFT tilapia fed 25% or 35% dietary protein was significantly higher (P<0.05) compared with red tilapia at either protein level. Increasing the dietary protein level by 10% led to a further 10% increase in the final weight of GIFT tilapia but no further increase was observed in the weight gain of red tilapia. Growth was influenced by the interaction between diet and tilapia genotype. Feed conversion ratios were 14% and 33% better in GIFT tilapia compared with red tilapia fed the 25% or 35% protein diet respectively. Protein efficiency ratio and net protein utilization were influenced by both diet and tilapia genotype, but not the interaction between the two. The apparent digestibility coefficients of dry matter and protein did not differ significantly between the two diets and tilapia genotypes but lipid digestibility was 1.2–4.7% higher for the GIFT tilapia. Tilapia whole‐body protein content was significantly influenced by both diet and tilapia genotype. The higher growth potential, better feed utilization efficiency and higher body protein content of GIFT tilapia compared with red tilapia should have a positive impact on tilapia farming in terms of production costs.     

不同饲料料型及投喂频率对斑点叉尾鮰幼鱼生长及肌肉品质的影响

本研究旨在探究不同饲料料型及投喂频率对斑点叉尾鮰(Ictalurus punctatus)生长及肌肉品质的影响。研究采用双因素试验设计,以饲料料型(膨化饲料,EF;颗粒饲料,PF)和投喂频率(2次/日,F2;3次/日,F3)为影响因素,共4个组,分别命名为EFF2、PFF2、EFF3和PFF3,每组3个平行,投喂8周,每天定时投喂两次(7:30和16:30)或3次(7:30, 12:00, 16:30)。结果显示,斑点叉尾鮰的末重和增重率受到投喂频率的影响,F2组显著高于F3组(P0.05);而饲料料型则对斑点叉尾鮰的生长无显著影响(P0.05)。颗粒饲料组的肌肉粗脂肪含量和内聚性、回复性显著低于膨化饲料组(P0.05),而硬度显著高于膨化饲料组(P0.05)。F3组的肌肉硬度、胶着性和咀嚼性则显著高于F2组(P0.05)。颗粒饲料组生肌调节因子5 (myogenic factor 5, Myf5)和肌肉生长抑制素(myostatin,MSTN)基因的相对表达量显著低于膨化饲料组(P0.05)。F3组肌肉中MSTN基因相对表达量显著高于F2组,而肌细胞生成素(myogenin,MyoG)基因相对表达量则呈现相反趋势。综上,投喂同种配方的膨化饲料和颗粒饲料对斑点叉尾鮰的生长效果相似,但是投喂颗粒饲料的斑点叉尾鮰硬度更大,而投喂膨化饲料则肌肉脂肪含量更高;斑点叉尾鮰的最佳投喂频率是每天2次。     

饲料中添加浒苔对凡纳滨对虾生长性能和肌肉品质的影响

试验旨在探究饲料中添加浒苔(Enteromorpha prolifera)对凡纳滨对虾(Litopenaeus vannamei)生长性能和肌肉品质的影响。在鱼粉含量分别为18% (A)和15% (B)的基础饲料中添加0%、3%、6%的浒苔(记为A0、A3、A6、B0、B3、B6),共6组等氮等能饲料,饲喂初始体重为(3.0 ± 0.1) g的凡纳滨对虾8周。结果显示：A0组具有最大增重率(654.2%)和最低饲料系数(1.61),在18% 、15%鱼粉饲料中添加3%浒苔,对虾体生长性能没有显著影响(P> 0.05),添加6%浒苔, 显著降低了增重率,升高了饲料系数 (P < 0.05),降低了虾体粗脂肪含量(P < 0.05);在18%、15%鱼粉饲料中添加3%、6%浒苔,显著降低了凡纳滨对虾的蛋白质效率、蛋白质沉积率、脂肪沉积率和蛋白质消化率(P < 0.05)。在肌肉品质方面,A3、A6、B6组肌肉硬度和咀嚼性均较A0、B0组显著提高(P < 0.05);饲料中添加6%浒苔显著提高了肌肉胶原蛋白含量,降低了肌肉煮失水率和冷冻失水率(P < 0.05);以上结果表明,在凡纳滨对虾饲料中添加3%浒苔,对虾体生长性能没有显著影响,添加6%浒苔,降低了虾体增重率和饲料利用,但可改善肌肉品质。     

The supplementation of nutrient additives in broad bean-based diet improved the growth of “crisped” grass carp,Ctenopharyngodon idellus

To investigate the effects of supplemental nutrient additives in broad bean-based diets on growth, flesh quality, and collagen gene expression of grass carp, five diets were prepared, including complete formula diet (control), soaked broad bean (SBB), and three broad bean-based diets containing 80% broad bean without (BBD1) or with the supplementation of methionine (BBD2), and methionine+vitamins+minerals (BBD3), and were fed to grass carp (171.9 ± 1.1 g), Ctenopharyngodon idellus, for 84 days. The results showed that broad bean-based diets significantly increased weight gain (WG) and reduced the feed conversion ratio (FCR) of grass carp when compared to the SBB (p < .05). The BBD3 group reached levels similar to the WG and FCR of the control group (p > .05). Grass carp fed the BBD3 diet had lower steaming loss of flesh, higher muscle fiber density, and higher collagen content in muscle and skin than the control (p < .05). The relative expressions of COL1A1 and COL1A2 mRNA in muscle and skin were significantly higher in the BBD3 and SBB groups than in other groups (p < .05). In conclusion, the combination of methionine, vitamins, and minerals in broad bean-based diets promoted the growth of “crisped” grass carp and improved flesh quality and collagen gene expression when compared to the control formula diet.