喷施ABA对小麦籽粒谷蛋白组分含量及GMP粒度分布的影响#br#

【目的】探讨喷施外源激素ABA引起小麦籽粒谷蛋白组分及谷蛋白大聚合体(glutenin macro-polymer, GMP)粒度分布的变化,为激素调控改善小麦籽粒品质提供理论依据。【方法】以冬小麦品种山农8355和山农15为试验材料,通过大田试验研究喷施外源ABA对小麦籽粒谷蛋白组分含量及GMP粒度分布的影响。【结果】在孕穗末期和籽粒形成期喷施ABA(浓度12 mg&#8226;kg-1,用量45 mL&#8226;m-2)均能提高籽粒粗蛋白及谷蛋白含量,同时,籽粒中高、低分子量不溶性谷蛋白含量均有所增加。特别是在孕穗期喷施ABA,山农8355和山农15两品种籽粒中高、低分子量不溶性谷蛋白含量与对照相比均达到显著水平,但喷施ABA对籽粒中可溶性谷蛋白含量影响相对较小。不论是孕穗期还是籽粒形成期喷施ABA均能增加GMP含量与产量,并改变籽粒中GMP的粒度分布,尤其是对其体积及表面积分布影响相对较大。相关分析表明,高、低分子量不溶性谷蛋白、GMP含量及GMP/Pr.比值均与GMP小颗粒(d<15 um)粒度分布呈现负相关,与大颗粒(d≥15 um)粒度分布的关系呈正相关。【结论】 喷施外源ABA可以改变籽粒中谷蛋白组分及GMP粒度分布,从而影响小麦籽粒品质。     

喷施ABA对小麦籽粒谷蛋白组分含量及GMP粒度分布的影响

【目的】探讨喷施外源激素ABA引起小麦籽粒谷蛋白组分及谷蛋白大聚合体(glutenin macro-polymer,GMP)粒度分布的变化,为激素调控改善小麦籽粒品质提供理论依据。【方法】以冬小麦品种山农8355和山农15为试验材料,通过大田试验研究喷施外源ABA对小麦籽粒谷蛋白组分含量及GMP粒度分布的影响。【结果】在孕穗末期和籽粒形成期喷施ABA(浓度12 mg.kg-1,用量45 mL.m-2)均能提高籽粒粗蛋白及谷蛋白含量,同时,籽粒中高、低分子量不溶性谷蛋白含量均有所增加。特别是在孕穗期喷施ABA,山农8355和山农15两品种籽粒中高、低分子量不溶性谷蛋白含量与对照相比均达到显著水平,但喷施ABA对籽粒中可溶性谷蛋白含量影响相对较小。不论是孕穗期还是籽粒形成期喷施ABA均能增加GMP含量与产量,并改变籽粒中GMP的粒度分布,尤其是对其体积及表面积分布影响相对较大。相关分析表明,高、低分子量不溶性谷蛋白、GMP含量及GMP/Pr.比值均与GMP小颗粒(d15 um)粒度分布呈现负相关,与大颗粒(d≥15 um)粒度分布的关系呈正相关。【结论】喷施外源ABA可以改变籽粒中谷蛋白组分及GMP粒度分布,从而影响小麦籽粒品质。     

小麦蛋白质组分含量与面团流变学性状的关系

利用国内外60份小麦种质材料,测定了面粉中清蛋白、球蛋白、醇溶蛋白、麦谷蛋白及剩余蛋白5种蛋白质组分含量和面团形成时间、稳定时间和吸水率3个主要流变学指标,揭示了蛋白质组分含量与不同小麦种质面团流变学性状的关系.结果表明:小麦5种蛋白质组分的变异幅度都较大,介于11.06%～37.41%之间,在小麦种质资源中存在丰富的...     

谷蛋白溶涨指数与面团特性和面条品质的关系

 采用谷蛋白溶涨指数 (SIG)法及新的蛋白质组分分离方法 ,分析了 2 5个中国小麦品种的蛋白质组分含量 ,并对面团物理特性、面条制作特性和蒸煮品质进行了评价。结果表明 ,不同溶涨时间 (min)SIG与不溶性谷蛋白含量呈极显著正相关 (r=0 .80 8～ 0 .86 7;P <0 .0 1) ,说明SIG可间接反映不溶性谷蛋白的含量。蛋白质含量、面筋指数、稳定时间、拉伸长度、拉伸能量与SIG5、SIG2 0显著正相关 (r=0 .5 16～ 0 .734;P <0 .0 5 ,0 .0 1) ,说明SI     

Swelling Index of Glutenin (SIG) Related to Protein Quality,Dough Characters and Noodle Quality

The swelling index of glutenin (SIG) and the protein fraction of 25 Chinese wheat varieties were studied with new protein fractions extracting method. The protein fractions compose of monomeric protein, soluble glutenin and insoluble glutenin. The relations between other protein index, dough character, and fresh noodle quality were also discussed. The SIG results at different time is positively and highly significantly related to the insoluble glutenin content (r=0.808 -0.867, P＜ 0.01). The SIG result can reflect the insoluble glutenin content. The protein content, gluten index, farinograph stability time, extensibility length and extensigramenergy were positively and significantly correlated with SIG5 and SIG20 (r=0.516- 0.734, P＜0.05, 0.01).SIG proved to be applicable in Chinese wheat dough evaluation. Fresh noodle making quality parameters were evaluated by fresh noodle length, thickness, maximum resistance to extension, extension area and extension distance, while cooked noodle texture was determined by cutting firmness, compression recovery, surface firmness and TPA by using a texture analyzer of TA.XT2i.The noodle cooking quality was significantly correlated with SIG value. The surface firmness and cutting firmness were more desirable for predicating the quality difference than TPA test and compression. Cooking loss and water absorption were negatively related to SIG value and insoluble content (r=-0.556 - - 0.787, P＜ 0.05, 0.01) . The results showed that SIG test was also suitable in evaluating noodle making and cooking quality in very small sample size, which was very important in wheat breeding programs. Therefore,SIG test, as a small scale test, is suitable to evaluate dough rheological properties for Chinese wheat varieties, and will be helpful in cereal research and wheat breeding program, especially, in early generations.     

Coupling effects of irrigation and nitrogen fertilization on grain protein and starch quality of strong-gluten winter wheat

Effects of irrigation and nitrogen fertilization on the grain yield, protein composition, protein quality, starch composition and starch pasting properties of a strong-gluten winter wheat were investigated in a high fertility field. Compared with non-irrigation treatment, grain yields under irrigation treatments were significantly increased, but the content of grain protein, monomeric protein and flour wet gluten was reduced. There were no significant differences in the above parameters between the irrigation treatments. Nitrogen application could significantly increase grain yield under low irrigation frequency (W₀ and W₁), while the neglected effect on yield was observed with high irrigation frequency (W₂ and W₃). With the increase of irrigation frequency, the glutenin content leveled off, but the changes of glutenin composition were not uniform, in which the soluble glutenin content was increased, while the insoluble glutenin content and polymerization index (the ratio of insoluble glutenin to total glutenin) were reduced. Both dough development time and stability time became shorter with the increased irrigation frequency. Nitrogen application improved the content of all grain protein fractions and grain quality, in which the increased degree in non-gluten protein (albumin and globulin) was higher than gluten protein (gliadin and glutenin), and the increased degree in soluble glutenin was found higher than that of insoluble glutenin. The interactive effects of irrigation and nitrogen on starch composition were significant. Starch content and amylopectin content was increased as irrigation frequency added in non-nitrogen treatment. Compared to non-irrigation treatment, irrigation significantly increased the starch content and the amylopectin content in nitrogen application treatment, but the starch and amylopectin content had no significant difference between irrigation treatments. Amylose content and the ratio of amylose to amylopectin were reduced while RVA indexes (peak viscosity, breakdown, final viscosity and setback) were increased as irrigation frequency was increased. Nitrogen application significantly improved the amylopectin content and decreased the amylose content in lower frequency irrigation, while the amylopectin content was decreased and the amylose content was enhanced by nitrogen application in higher frequency irrigations. __________ Translated from Plant Nutrition and Fertilizer Science, 2007, 13(3): 361–367 [译自: 植物营养与肥料学报]     

灌水时期和灌水量对小麦耗水特性、籽粒产量及蛋白质组分含量的影响

 【目的】研究高产条件下灌水时期和灌水量对小麦的耗水特性和籽粒蛋白质组分含量的影响,为小麦节水高产优质栽培提供理论依据。【方法】设置不同灌水时期和灌水量的处理,采用反相高效液相色谱（RP-HPLC）分析方法对籽粒蛋白质进行分离量化,研究不同水分处理对小麦耗水量、水分利用率、籽粒产量、籽粒品质和籽粒蛋白质组分含量的影响。【结果】随着灌水量的增加,灌水量占农田耗水量的百分率提高,降水量和土壤贮水消耗量占农田耗水量的百分率降低。减少灌水量,促进小麦对土壤贮水的利用,提高小麦在0～100 cm各土层的土壤耗水量。降低农田耗水量、提高水分利用率是实现节水高产栽培的有效途径。拔节期和开花期分别灌水60 mm的处理在两年度生长季均获得了最高的水分利用率;在2004－2005年生长季W1处理的籽粒产量与W2无显著差异,但显著高于W0处理;在2005－2006年生长季W'2处理的籽粒产量与W'3无显著差异,但显著高于W'0、W'1处理。水分对小麦籽粒蛋白质组分含量具有重要的调控作用。在2004－2005年生长季,与W2处理相比,W1处理的籽粒醇溶蛋白含量降低,HMW-GS含量和谷蛋白含量升高,籽粒蛋白质含量和湿面筋含量升高,面团形成时间和面团稳定时间延长,有利于强筋小麦济麦20籽粒品质的改善。在2005－2006年生长季,随灌水量增加,籽粒HMW-GS含量、谷蛋白含量有先升高后降低的趋势,以W'2处理最高,与籽粒蛋白质含量、面团形成时间和面团稳定时间的变化趋势一致。【结论】本试验条件下,拔节期和开花期分别灌水60 mm是兼顾节水、高产、优质的最优处理。     

氮肥与硫肥施用对相同HMW-GS组分春小麦谷蛋白大聚合体含量的影响

黑龙江是中国重要的强筋春小麦产区,虽然培育出了多个优质强筋麦品种,但是如何通过合理的施肥技术等保证小麦品质已成为黑龙江小麦生产的重要问题。作为品质鉴定指标,麦谷蛋白大聚合体的含量比蛋白质和湿面筋含量更具代表性。为此,在相同HMW-GS背景下研究了氮肥与硫肥施用对小麦谷蛋白大聚合体的含量的影响。试验分设4个施肥处理（未施肥、单施纯硫50 kg.hm-2、单施纯氮150 kg.hm-2、纯硫50 kg.hm-2＋纯氮150 kg.hm-2）。结果表明：氮肥与硫肥的不同处理对小麦籽粒的蛋白质和麦谷蛋白大聚合体有明显影响。施硫肥、氮肥以及2种肥料混施提高了上述2个品质指标及其比值。综合研究表明,在3种处理方式上,氮肥与硫肥混合施用对蛋白质、麦谷蛋白大聚合体影响最大。因此,生产上在提高氮肥用量的同时,可通过增施硫肥,达到小麦高产优质的目的。     

小麦谷蛋白大聚合体研究现状与进展

小麦是重要的粮食作物。蛋白质含量水平及其组分影响小麦品质。其中麦谷蛋白大聚合体是其组成中分子量最大的一部分蛋白质,其含量反映着麦谷蛋白聚合体的粒度分布。详细介绍了麦谷蛋白大聚合体的分离方法,麦谷蛋白大聚合体含量及粒度分布对小麦品质的影响及其影响的作用机制。     

硫肥施用方式对冬小麦灌浆期籽粒蛋白含量动态的影响

[目的]为冬小麦品质改良和优质高效栽培提供理论依据。[方法]在大田条件下,研究2种硫肥施用方式对冬小麦中筋力型品种豫麦49和强筋力型品种豫麦66灌浆期籽粒蛋白质含量和麦谷蛋白含量影响。[结果]施用硫肥能提高2种筋力型冬小麦品种籽粒蛋白质含量和麦谷蛋白含量,尤其对中筋型小麦品种豫麦49效果更明显。[结论]针对不同筋力型品种,结合不同要求,采取合理硫肥施用技术可以起到提高小麦籽粒蛋白质含量和麦谷蛋白含量的作用。     

Studies on Single Cell Culture in vitro in Wheat--The variation of grain protein content and its fractions from regenerated plants

On the basis of previous studies dealing with the variation of major agronomic and yield characteristics of regenerated plants derived from single cell culture in vitro of common wheat (Triticum aestivum L.Cultivar NE 7742),the grain protein content and its fractions from regenerated plants with stable agronomic characteristics were studied from 1992 to 1995.The results showed that the variation of grain protein content and its fractions in somaclones from single cell culture in vitro were very significant and the range was very wide (11.53-17.70%).Several types of variation were found in the studies,espercially the type with higher protein content than that of cultivar NE 7742(non-culture parent).Among them.-20.69% of lines the grain protein content was significantly higher than that of NE 7742 and combined with high yielding potential.The tendency of variation of the four protein fractions showed that the variation of albumin was not obvious and maintained the same level as NE7742,the content of gliadin increased in some somaclones and decreased in others.However,the percentages both globulin and glutenin tended to increase.The variation of total amount of structural protein and the ratio between globulin and glutenin tended to increase.the variation of total amount of structural protein and the ratio between globulin and albumm was mainly influenced by globulin under the condition of culture in vitro.The variation of total amount of storage protein and the ratio between gliadin and glutenin was mainly by glutenin.The results mentioned above bemonstrated that the induction and screening of somaclonal variation could be an effective way in wheat improvement in combining high protein content with high yield.     

影响小麦面粉白度的品质指标分析

 【目的】系统研究影响小麦面粉白度的品质指标，对于作物遗传育种筛选高白度优质资源，为工业化面制食品加工提供优质原粮信息具有重要意义。【方法】在对黄淮冬麦区56个小麦品种的白度、破损淀粉、黄色素、PPO活性、面团流变学特性、蛋白质品质等指标系统测定的基础上，以基因环境互作软件对影响小麦面粉白度的品质指标进行系统分析。【结果】面粉白度与蛋白质含量、破损淀粉含量、吸水率呈极显著负相关（r=-0.488、-0.673、-0.732），与面粉溶涨体积呈极显著正相关（r=0.762），与PPO活性和黄色素含量关系不显著。【结论】经过软件分析，筛选出白度高且面粉溶涨体积大的品种有绵阳31、绵阳26、西农2611、陕农757、中原98-68；白度高且破损淀粉少的品种有绵阳25、安农9866、绵阳26、绵阳31、滨麦3号；白度高且吸水率高的品种有绵阳26、滨麦3号、高优511、新麦9-988、绵阳31；白度高且蛋白质含量高的品种有高优511、内乡118、郑麦002、山农664、西杂5号；白度高且谷蛋白含量高的品种有高优511、西农2611、西杂5号、陕农757、中原98-68；白度高且多酚氧化酶活性低的品种有绵阳26、亿高1号、高优511、中原98-68、绵阳25等，这些品种可以作为高白度小麦优质资源。     

小麦谷蛋白大聚合体的研究进展

小麦谷蛋白大聚合体﹙GMP﹚的含量及粒度大小相对分布﹙用不溶性聚合体蛋白占总聚合体蛋白含量的百分数表示﹐即UPP%﹚与小麦烘烤品质的关系密切。在此,对近年来小麦谷蛋白大聚合体的结构特征、测定方法及其与小麦品质性状关系的研究现状进行了综述。     

麦麸添加量和粒度对面团揉混特性的影响

 【目的】研究麦麸对面团流变学特性的影响规律及机理,为麦麸膳食纤维在食品加工中的应用提供依据。【方法】以山农优麦2号小麦面粉为试验材料,通过添加4个粒度（0.16—0.43、0.43—1.0、1.5—2.0、2.0—2.5 mm）,4个添加量（5%、10%、15%、20%）的麦麸,研究麦麸添加量和粒度对面团揉混特性的影响。【结果】随着麸皮添加量的增加,中线峰值高度、中线峰值宽度、8 min中线高度、8 min中线积分面积、中线曲线尾高度和中线曲线尾积分面积显著降低,而8 min带宽、中线曲线尾宽度和中线峰值右侧切线斜率显著增加;添加量处理间对中线峰值时间、中线峰值积分面积和中线峰值左侧切线斜率的影响差异不显著。随着麸皮粒度的增大,中线峰值时间、8 min带宽和中线曲线尾宽度、中线峰值右侧斜率显著增加,但麸皮粒度处理间对其它揉混特性指标的影响差异不显著。【结论】麦麸纤维显著降低面团的中线峰值高度、8 min中线高度、中线峰值积分面积和8 min中线积分面积;而显著增加中线峰值时间和8 min带宽、中线曲线尾宽和中线峰值右侧切线斜率。面团中线峰值时间、8 min带宽和中线曲线尾宽度、中线峰值右侧切线斜率随着麸皮粒度的增大显著增加。     

Variations in the quality parameters and gluten proteins in synthetic hexaploid wheats solely expressing the Glu-D1 locus

This study evaluated the quality potential of seven synthetic hexaploid wheats (2n=6x=42, AABBDD) expressing only allelic variation at Glu-D1 of Aegilops tauschii (SHWSD). Major quality parameters related to dough strength, gluten proteins (including high-molecular-weight glutenin subunits (HMW-GS) and low-molecular-weight glutenin subunits (LMW-GS), gliadins), and their ratios between SHWSD and the weak gluten wheat control Chuannong 16 (CN16) were measured in at least three environments (except STD7). The zeleny sedimentation value (ZSV), dough development time (DDT), dough stability time (DST), and farinograph quality number (FQN) of SHWSD were considered stable under different environments, with their respective ranges being 8.00–17.67 mL, 0.57–1.50 min, 0.73–1.80 min, and 9.50–27.00. The ZSV, DDT, DST, and FQN of SHWSD were smaller than those of CN16, suggesting that SHWSD had a weaker dough strength than CN16. Although SHWSD had a lower gluten index than CN16, its wet and dry gluten contents were similar to or even higher than those of CN16 in all environments tested. The protein content of grains (12.81–18.21%) and flours (14.20–20.31%) in SHWSD was higher than that in CN16. The amount of HMW-GS in SHWSD sharply decreased under the expression of fewer HMW-GS genes, and the LMW-GS, gliadins, and total glutenins were simultaneously increased in SHWSD in comparison with CN16. Moreover, SHWSD had higher ratios of LMW-GS/glutenin and gliadin/glutenin but a lower ratio of HMW-GS/glutenin than CN16. These results provide necessary information for the utilization of SHWSD in weak-gluten wheat breeding.     

外源蛋白添加对全麦面团特性和面包品质的影响

【目的】研究两种外源蛋白添加对全麦面团混合特性、流变学特性和微观结构及对全麦面包品质的影响,为进一步利用外源蛋白提升全麦食品品质及其新产品的开发提供依据。【方法】利用混合实验仪、动态流变仪和扫描电子显微镜分析外源蛋白（蛋清粉（EW）和酪蛋白酸钠（SC））添加对全麦面团热机械学、动态流变学和微观结构的影响,结合全麦面包的制作分析其对面包比容和质构特性的影响。【结果】随着（EW）添加量的增加,全麦面团吸水率降低,形成时间和稳定时间增加,加热过程中全麦面团的峰值黏度和回生值升高。全麦面团的弹性模量（G'）和黏性模量（G"）持续降低,损耗角正切值（tan δ）持续上升。面团微观结构得到有效改善,内部孔洞显著减少,面团结构连续均匀,很好地弥补了麸皮对面团的破坏;全麦面包的比容增大,面包的内瓤结构疏松柔软。全麦面包的硬度下降,咀嚼度降低,弹性、黏聚性和回复性变化不显著。随SC添加量的增加,全麦面团的吸水率和蛋白弱化度增加,形成时间和稳定时间先增加后降低,加热过程中,全麦面团的峰值黏度随SC添加量增加而降低,回生值变化趋势相反;当SC添加量大于1%时,全麦面团的G'和G"上升;面团微观结构逐渐变得致密,孔洞和缝隙逐渐减少;全麦面包的比容随SC添加量增加先增大后减小,内瓤结构相对紧密;全麦面包硬度和咀嚼度逐渐增加,黏聚性和回复性逐渐降低,弹性变化不显著。【结论】蛋清粉的添加一定程度上可以代替谷朊粉,有效改善全麦面包品质;而酪蛋白酸钠的使用要考虑其自身的高吸水率。     

RVA and Farinograph Properties Study on Blends of Resistant Starch and Wheat Flour

Resistant starch （RS） is the undigested starch that passes through the small intestine to the large intestine. As a functional low calorie additive, it has special applications in the food industry. Rapid visco analysis （RVA） and the Brabender farinograph were used to study the pasting properties and the viscoelasticity of blends of RS （RS3 and RS2） and three wheat flours. The wheat flours represented strong gluten wheat （SGW）, intermediate gluten wheat （IGW）, and weak gluten wheat （WGW） flours, at different levels of RS substitution （0, 5, 10, 15, and 20%）. The influence of RS3 on the control wheat flours and RS-wheat flour blends were consistent with those of RS2. The peak, trough, and final viscosities of RS3-wheat flour blends were higher than those of the corresponding RS2-wheat flour blends. The peak, trough, breakdown, final, and setback viscosities ofwheat-RS blends decreased with an increase in resistant starch contents from 0 to 20% in the blends. The 0-20% RS-wheat flour blends were all able to form doughs. The dough development times, dough stabilities, dough breakdown times, and farinograph quality numbers for the RS-wheat flour blends decreased as the RS proportion in the blends increased. The values for RS-SGW flour blends were the highest, followed by RS-IGW and then RS-WGW flour blends. The water absorption values for RS-wheat flour blends and the mixing tolerance index for RS-WGW flour blends were found to increase significantly with an increasing proportion of RS from 0 to 20%, but the mixing tolerance index for RS-SGW and RS-IGW flour blends showed no significant differences amongst the different ratios. Correlation analysis showed that the Farinograph quality number was highly positively correlated with dough breakdown time, dough stability, and dough development time （r= 1.000, 0.958, 0.894）, and highly negatively correlated with the mixing tolerance index （r =-0.890）. Data from this study can be used for the development of dough-based products. It also pr     

磷氮配施对小麦籽粒蛋白质组分含量和面团特性的影响

 【目的】试图阐明磷氮配施对小麦籽粒蛋白质品质的影响。【方法】以蛋白质品质差异较大的小麦强筋型品种藁城8901和弱筋型品种山农1391为材料,进行磷氮配施田间试验,测定籽粒蛋白质及其组分含量的动态变化和面团的流变学特性。【结果】藁城8901在施225 kg•ha-1氮肥水平下,与不施磷肥相比,增施磷肥的清蛋白、球蛋白、醇溶蛋白、谷蛋白和总蛋白及谷蛋白大聚合体含量提高,吸水率、面团形成时间、稳定时间和评价值增加,公差指数降低。施P2O5 240 kg•ha-1处理较施P2O5 120 kg•ha-1处理的相应效果降低。藁城8901在不施氮肥、山农1391在不施氮肥及施225 kg•ha-1氮肥水平下,随磷肥用量增加清蛋白和球蛋白含量提高,醇溶蛋白、谷蛋白、总蛋白和谷蛋白大聚合体含量降低;吸水率、面团形成时间、稳定时间和评价值下降,公差指数升高。【结论】不同品种在不同供氮水平条件下,增施磷肥时,小麦籽粒蛋白质及其各组分含量、面团流变学特性等变化的趋势不同。对强筋型品种藁城8901,在保证氮肥供应的基础上适量增施磷肥可提高其加工品质;对弱筋型品种山农1391,增加磷肥用量有利于其加工品质的改善。     

Changes in Activities of Antioxidant-Related Enzymes in Leaves of Resistant and Susceptible Wheat Inoculated with Rhizoctonia cerealis

In this study, the changes in activities of peroxidase (POD), glutathione S-transferase (GST), catalase (CAT), and superoxide distmuase (SOD) in leaves of four wheat cultivars, including resistant cultivars Shannong 0431 and Shanhongmai, a mild-susceptible cultivar Lumai 21, and a susceptible cultivar Wenmai 6, were investigated to explore if these antioxidantrelated enzymes were involved in wheat defense response to Rhizoctonia cerealis attack. Results showed that the activities of these enzymes in different wheat cultivars varied greatly after challenging with R. cerealis. The PODactivities in resistant cultivars Shannong 0431 and Shanhongmai increased much quicker after the inoculation and displayed much higher activity than that in susceptible cultivar Wenmai 6. In contrast, POD activity showed a slow accumulation and a delayed peak time. The activities of GST and CAT in Shannong 0431 and Lumai 21 increased obviously within 48 h post inoculation (hpi), whereas SOD activity decreased dramatically within 24 hpi in Shannong 0431 and 24-48 hpi in Lumai 21. In susceptible cultivar Wenmai 6, the SOD and CAT activities didn't change obviously, whereas the activity of GST was decreased. The activities of CAT and GST in Shanhongmai did not show significant difference compared with those in Wenmai 6. Results suggested that POD activity may be positively associated with wheat defense to R. cerealis.