相同储藏条件下同年度不同品种小麦品质的变化

选用5个同一生产年度的不同品种小麦,在同一储藏条件下对其容重、水分、面筋吸水量、粘度、降落数值和发芽率等品质指标进行测定,讨论各指标与储藏时间的关系及品种差异对各指标的影响,分析各指标之间的相关性。结果显示:随着储藏时间的延长,小麦容重、水分和粘度的变化趋于稳定,面筋吸水率和发芽率呈降低趋势,降落数值呈增大趋势;不同小麦品种的容重、发芽率变化差异不大;储藏1年后,不同小麦品种的水分、面筋吸水率、降落数值变化差异较大;容重与其它五个指标都存在一定的相关性,这也是选择容重作为小麦等级鉴定的必要性。     

小麦水分含量对容重及硬度的影响

容重是国内外粮食定等及评价粮食工艺品质的主要指标,小麦籽粒硬度是国内外小麦市场分类和定价的重要依据之一,通过改变小麦水分含量,判定其对小麦容重及硬度的影响。结果表明,在一定的水分范围之内,小麦容重与水分含量呈负相关,小麦籽粒硬度与水分含量呈正相关。     

高大平房仓空调控温结合氮气气调对小麦品质的影响

对4个高大平房仓储粮小麦在度夏期间进行空调控温。在此基础上选取2个仓进行氮气气调，研究了单独空调控温以及空调控温结合氮气气调储粮过程中小麦的品质变化情况。研究结果表明：仅单独空调控温时，随着储藏时间的增加，小麦的害虫数量不断增加，而小麦的害虫数量在氮气气调后则开始呈现显著降低的趋势；两种储粮方式下小麦的水分含量、容重和面筋吸水率均随着储藏时间的增加而降低，其中氮气气调的小麦水分含量、容重和面筋吸水率降低程度稍低于仅空调控温的小麦；小麦的色泽、气味和呕吐毒素含量在储藏期间未发现明显改变，表明应用氮气气调未对其产生显著影响。     

快速谷物分析仪测定小麦水分和容重的适用性验证

对快速谷物分析仪测定小麦的水分含量和容重进行了适用性验证。通过快检设备对小麦的水分和容重进行了测定,将结果与国标法进行比较和分析,验证设备的准确性、重复性和稳定性是否达到仪器技术指标和国标规定的要求。准确性验证通过SPSS配对T检验,重复性验证通过■X■2检验,稳定性通过单因素方差分析。结果表明:快速谷物分析仪校准后,测定小麦水分含量和容重时,其准确性、重复性、稳定性均能达到仪器的技术指标和国标规定的要求,适用于小麦水分和容重的快速测定。     

伴生麦与普通小麦的形态、产量和品质性状差异分析

为研究伴生麦与普通小麦在形态、产量和品质性状间的差异,以普通小麦品种矮抗58和3个伴生麦品种W14、W15、W18为供试材料进行研究。设置水分处理(主区)和施氮量(副区)的裂区试验,其中水分处理设正常水分和渍水2个水平,施氮量设置不施氮(N_0)、正常施氮(225 kg/hm~2,即N_(225))和过量施氮(300 kg/hm~2,即N_(300))3个水平,结果表明,正常水肥管理下,伴生麦的分蘖数较多且茎粗较小,其株高是普通小麦的1.5～2.4倍,而产量却不足普通小麦的1/2,此外,伴生麦品种W14、W15、W18的籽粒蛋白质含量、湿面筋含量和吸水量等品质指标均高于普通小麦。施氮可提高普通小麦及伴生麦的穗粒数、产量、籽粒蛋白质含量、湿面筋含量、吸水量、形成时间、稳定时间、出粉率、拉伸能量、恒定变形拉伸阻力、延伸度和最大拉伸阻力,正常施氮(225 kg/hm~2)使普通小麦和伴生麦千粒质量、产量、容重和硬度达到最高。水分对普通小麦和伴生麦的形态及产量影响较小,但对籽粒品质影响显著。综上所述,水分、品种及氮素对普通小麦和伴生麦的籽粒品质具有显著影响,是决定普通小麦及伴生麦籽粒品质的关键因素。     

剪根与水分胁迫对小麦单根和细胞导水率及TaPIP基因表达的影响

剪去小麦部分根系能瞬间打破其水分平衡,研究根系导水特性对剪根的响应有助于解释静水压对作物根系吸水的调节机制。通过对苗期小麦(Triticum aestivum)剪根与水分胁迫处理,用压力探针技术测定单根和细胞两种尺度上的根导水特性变化,以及根中TaPIP1;2和TaPIP2;5的转录调节变化。结果显示,剪根处理或水分胁迫处理使叶片蒸腾速率和气孔导度均显著低于对照,而单根导水率和细胞导水率均与对照无显著差异。剪根处理的叶片蒸腾速率、气孔导度、叶水势、单根导水率和细胞导水率均显著高于水分胁迫处理,而剪根且水分胁迫处理的各参数均显著低于其他处理。各处理的单根导水率与细胞导水率显著正相关。各处理根中TaPIP1;2和TaPIP2;5相对mRNA含量的变化规律与单根和细胞导水率的变化规律相似。剪根处理显著上调了TaPIP1;2和TaPIP2;5转录,水分胁迫处理显著下调了其转录,但TaPIP1;2和TaPIP2;5在剪根且水分胁迫处理中的转录水平最低。这些结果表明,小麦的根导水特性在单根尺度和细胞尺度上具有一致性;剪根短期内能够增加小麦幼苗的水分敏感性。推测TaPIP1;2和TaPIP2;5参与了静水压对小麦根导水特性的调节过程。     

不同储藏条件对小麦专用粉品质的影响研究

采用正交实验方法,研究储藏温度、储藏水分、储藏方式与储藏时间四个因素对小麦专用粉储藏期间面团稳定时间、面筋吸水量、脂肪酸值和降落数值的影响。结果显示:在储藏时间180 d内,储藏温度仅对小麦专用粉的降落数值指标影响显著,呈现显著正相关;储藏时间仅对小麦专用粉的脂肪酸值指标影响显著,呈现显著正相关;储藏水分与储藏方式对小麦专用粉的面团稳定时间、面筋吸水量、脂肪酸值和降落数值指标影响均不显著。     

新型农户专用粮仓储粮品质变化研究

采用PVC软体仓作为新型农户专用仓,小麦在10个月储存期内水分含量变化≤0.6%,与原始水分无显著差异。小麦容重损失率均值为1.96%,干容重损失率均值2.1%,虫蚀粒率≤0.3%,霉变粒率≤0.1%,面筋吸水率变化率均值9.4%,平均储粮损失率为0.506%,干物质平均损失率为0.318%。由此可见PVC仓具有良好的防潮、防鼠、密闭、隔热等性能,能够有效地减少农户储粮过程中由粮食生虫、发热霉变、鼠咬等因素引起的储粮损失,起到减损保质的效果。     

水分胁迫对不同筋型小麦籽粒品质性状的影响

为了了解不同水分胁迫对不同品质类型小麦主要品质性状的影响,试验选用3个有代表性的强、中、弱筋春小麦品种,在防渗池中进行种植。研究结果表明：小麦籽粒主要品质性状在不同筋型间差异达到极显著水平,在不同水分条件下也存在显著差异。在水分胁迫下,湿面筋含量、粗蛋白含量以及沉淀值均呈显著或极显著下降趋势,而膨胀势显著增大。在试验条件下品质性状间的相关分析结果表明,不同品种间粗蛋白含量与湿面筋含量、沉淀值呈极显著正相关。因此在小麦生态育种和调优栽培研究中,需要重视水分因子对小麦籽粒品质性状影响的不同步性。     

玉米秸秆基纤维素保水剂对土壤持水性能及冬小麦根系生长的影响

为探讨自制的玉米秸秆基纤维素保水剂(SAP)在农业生产上的应用潜力,利用扫描电镜表征了SAP吸水前后形貌变化,探究了SAP施用量对土壤持水性能和不同程度水分胁迫下冬小麦根系生长的影响。结果表明：SAP在土壤中吸水膨胀后降低了土壤容重,土壤最大水分含量较不施SAP显著提高19.1%~67.6%,同时降低了前3天土壤水分蒸发速率。轻度（60%~70%田间持水量）和中度（40%~50%田间持水量）水分胁迫下,施用0.4% w/w SAP处理冬小麦总根长提高15.9%和24.7%,根系活力提高29.6%和43.5%,但充足供水（75%~90%田间持水量）时施用SAP对冬小麦根系活力无显著影响。因此,玉米秸秆基纤维素保水剂施入土壤后能够改善土壤持水性能,从而缓解水分胁迫对冬小麦根系生长的抑制效应。     

A note on the origin of Kalanchoë x vadensis Boom & Zeilinga (Crassulaceae)

Summary K x vadensis is a hybrid of K. blossfeldiana and K. marmorata obtained after doubling the number of chromosomes.     

Mechanisms and diversity of resistance to sorghum shoot fly,Atherigona soccata

Sorghum shoot fly, Atherigona soccata, is one of the important pests of postrainy season sorghums. Of the 90 sorghum genotypes evaluated for resistance to this pest, RHRB 12, ICSV 713, 25026, 93046 and 25027, IS 33844‐5, Giddi Maldandi and RVRT 3 exhibited resistance in postrainy season, while ICSB 463, Phule Anuradha, RHRB 19, Parbhani Moti, ICSV 705, PS 35805, IS 5480, 5622, 17726, 18368 and 34722, RVRT 1, ICSR 93031 and Dagidi Solapur showed resistance in rainy season, suggesting season‐specific expression of resistance to A. soccata. ICSB 461, ICSB 463, Phule Yasodha, M 35‐1, ICSV 700, 711, 25010, 25019 and 93089, IS 18662, Phule Vasudha, IS 18551 and 33844‐5 and Barsizoot had fewer deadhearts than plants with eggs across seasons, suggesting antibiosis as one of the resistance mechanism. Five genotypes exhibited resistance with high grain yield across seasons. Correlation, path and stepwise regression analyses indicated that leaf glossiness, seedling vigour, trichome density, oviposition and leaf sheath pigmentation were associated with the expression of resistance/susceptibility to shoot fly, and these can be used as marker traits to select and develop shoot fly‐resistant sorghums.     

Reaction of tritordeum to Fusarium culmorum and Septoria nodorum

Summary Hordeum chilense is a wild barley extensively used in wide crosses in the Triticeae. It could be a valuable source of resistance to Fusarium culmorum and Septoria nodorum. Some H. chilense x Triticum spp. amphiploids, named tritordeums, were more resistant than the parental wheat line to these diseases, others were not. Average contents of ergosterol and deoxynivalenol (DON) suggested that resistance to colonization by Fusarium was the highest for Hordeum chilense, followed by tritordeum and wheat in decreasing order. In particular, the H. chilense genotypes H7 and H17 enhanced the wheat resistance to F. culmorum in its tritordeum offsprings. Resistance to S. nodorum in tritordeum was not associated with tall plant height. There is sufficient genetic variation for resistance to F. culmorum and S. nodorum among tritordeum to allow the breeding of lines combining short straw and resistance to both diseases.     

Disease resistance in protected crops and mushrooms

Summary Cultivars of tomatoes, cucumbers, lettuce and peppers have been bred for resistance to one or more pathogens. Some tomato and cucumber cultivars have resistance to a wide range of diseases. Resistance has been transient in many cases and a succession of cultivars with new genes or new combinations of resistance genes has been necessary to maintain control. There has been a number of notable exceptions and these have included durable resistance to such pathogens asFulvia fulva and tomato mosaic virus. With lettuce the resistance situation is complicated by the occurrence of fungicide resistant pathotypes. There are no strains ofAgaricus bisporus purposely bred for disease resistance.In protected flower crops only resistance to Fusarium wilt in carnations has been purposely bred but differences in disease resistance are apparent in cultivars of many ornamental crops. This is particularly so in chrysanthemums where there are cultivars with resistance to many of the major pathogens. Similar situations occur with other flower crops and pot plants. Cultivars of some species have not been systematically investigated for resistance.The need for genetic resistance will increase with the further reduction, in the limits on pesticide use and an increasing public awareness and importance of pesticide pollution.ADAS is an executive agency of the Ministry of Agiculture, Fisheries and Food and the Welsh Office.     

Genetic constitution and diversity in four narrow endemic redwoods from the family Cupressaceae

The genetic constitution and diversity of four relictual redwoods are discussed in this review. These include monotypic genera of the family Cupressaceae: coast redwood (Sequoia sempervirens), giant sequoia (Sequoiadendron giganteum), dawn redwood (Metasequoia glyptostroboides), and alerce (Fitzroya cupressoides). All four species are narrow endemics, share a number of common phenotypic traits, including red wood, and are threatened species. Fossil history suggests that the ancestors of redwoods probably originated during the Cretaceous and Tertiary periods and flourished thereafter for millions of years. Towards the end of the Tertiary period began their decline and struggle for existence that continued during the subsequent geologic upheavals and climate changes, until the survival of the present-day redwoods in the current restricted locations in the world (USA, China, and South America). Although two species, Sequoiadendron and Metasequoia, are diploids (2n = 22), and the other two are polyploids: Fitzroya a tetraploid (2n = 4x = 44), and Sequoia a hexaploid (2n = 6x = 66); they all share the same basic chromosome number x = 11. The genome size in the hexaploid Sequoia is one of the largest (31,500 MB) in the conifers, while the genome sizes of diploid Metasequoia and Sequoiadendron are about one-third (~10,000 MB) of Sequoia. Genetic diversity in the redwoods is lower than most other gymnosperms, except in Sequoia, which seems to rank near the upper quarter of the coniferous forest trees. Genomic research is sparse in the redwoods, and should be pursued for a better understanding of their genome structure, function, and adaptive genetic diversity.     

The induction in vitro of adventitious shoots in Rosa

Summary Adventitious shoots were formed on excised leaves, roots and callus of Rosa persica x xanthina and on excised leaves of R. laevigata and R. wichuraiana on culture media that included BAP and NAA as growth regulators. Shoots formed freely on freshly cultured callus of R. persica x xanthina but their production declined in successive cultures and ceased after twelve weeks. Transplantation to soil was improved by rooting plantlets in cellulose plugs in vitro and transferring plantlets to soil while still in the plugs.     

Distribution,ecology and reproductive biology of wild tomatoes and related nightshades from the Atacama Desert region of northern Chile

Over the past 20 years, several expeditions were made to northern Chile to collect populations of wild tomatoes (Solanum chilense, S. peruvianum) and allied nightshades (S. lycopersicoides, S. sitiens), and obtain information about their geographic distribution, ecology and reproductive biology. Restricted mainly to drainages of the Andean and the coastal cordillera, populations are geographically fragmented. The two nightshade species are rare and threatened by human activities. Adaptation to extreme aridity and soil salinity are evident in S. chilense and S. sitiens (the latter exhibits several xerophytic traits not seen in the tomatoes) and to low temperatures in S. lycopersicoides and S. chilense. All tested accessions are self-incompatible, with the exception of one S. peruvianum population collected at the southern limit of its distribution. Several distinguishing reproductive traits—anther color, attachment, and dehiscence, pollen size, and flower scent—suggest S. sitiens and S. lycopersicoides attract different pollinators than S. chilense and S. peruvianum. The four Solanum spp. native or endemic to Chile provide a variety of novel traits which, through hybridization and introgression with cultivated tomato, could facilitate development of improved varieties, as well as research on a variety of basic topics, including plant-pollinator interactions, abiotic stress responses, and evolution of reproductive barriers.     

The exploration of genetic resources of Portuguese cabbage and kale for resistance to several Brassica diseases

Summary Twenty three accessions of nine Portuguese cabbage and kale land races from different geographic origins were tested at the seedling stage for resistance to several important brassica diseases. Resistance to downy mildew (Peronospora parasitica), expressed as necrosis of the cotyledon mesophyll, was found in all the accessions. Type A resistance to cabbage yellows (Fusarium oxysporum f. sp. conglutinans race 1) was present in most of the landraces. Resistance to clubroot (Plasmodiophora brassicae race 6) was found in one accession of the Portuguese tree kale. High resistance to blackleg (Leptosphaeria maculans) and white rust (Albuco candida) was not detected, although several accessions showed 20 to 30% of plants with intermediate expression of resistance. All Portuguese cole accessions were susceptible to blackrot (Xanthomonas campestris pv. campestris).     

Production and characterization of intergeneric diploid cybrids derived from symmetric fusion between Microcitrus papuana Swingle and sour orange (Citrus aurantium)

Plants were regenerated from intergeneric somatic hybridization between embryogenic protoplasts of Microcitrus papuana Swingle and leaf-derived protoplasts of sour orange (Citrus aurantium L.) via electrofusion. The regenerated plants were morphologically similar to the leaf parent in growth vigor, leaf and branch structure. FCM analysis showed that they were diploids. Simple-sequence-repeat (SSR) and cleaved-amplified-polymorphic-sequence(CAPS) were employed for hybridity characterization. SSR banding patterns of the regenerated plants were identical to the leaf parent, sour orange, indicating that they possessed nuclear component derived from sour orange. DNA amplification with chloroplast and mitochondrial universal primers, followed by restriction endonuclease digestion, revealed polymorphism between the fusion parents. Therefore, this method was used to determine the cytoplasmic compositions of the regenerated plants. Banding patterns for all the polymorphic primer/enzyme combinations of the regenerated plants were similar to those of the embryogenic parent, M. papuana, suggesting that only the cytoplasmic components derived from the embryogenic parent were present in the regenerated plants. FCM, SSR and CAPS demonstrated that intergeneric diploid cybrids have been successfully obtained by symmetric fusion. Related results concerning nuclear and cytoplasmic composition of previous diploid somatic hybrids and potential mechanism for regeneration of such kind of plants are discussed herein. This revised version was published online in July 2006 with corrections to the Cover Date.     

Breeding barriers between the cultivated strawberry, Fragaria × ananassa, and related wild germplasm

Five-hundred interspecific and intergeneric crosses were performed among accessions of the wild strawberries Fragaria vesca(2x), Duchesnea indica (8x), Potentilla tucumanensis (2x) and 9 genotypes of the cultivated strawberry, Fragaria×ananassa (8x), following an incomplete diallele mating design. Crosses between D. indica and F.×ananassa produced many putative hybrids when D. indica was used as female but a few achenes and plants when used as male; therefore, pollen-pistil compatibility relations were analyzed by fluorescence microscopy in this direction of the cross. Of the genotypic combinations, 78.6% were incompatible at the stigma level and 17.2% at the first third of the style. Only 3.6% were pollen-pistil compatible and produced fruits with achenes (seven did not germinate or originated short-lived plants and nine produced normal plants). F.vesca×F.×ananassa crosses produced 35 hybrid achenes but only 14% germinated, yielding short-lived plants; histological analyses revealed that inviable seeds had less developed (or collapsed) endosperms and smaller embryos than control plump F. vesca seeds. P.tucumanensis was only used as male, with negative results. These species and genera are partially isolated by a complex system of pre- and post-zygotic barriers. Knowledge of their nature would allow the breeder to devise strategies to put the genetic variability available in the group into a useful form. This revised version was published online in July 2006 with corrections to the Cover Date.