Genetic variation,heritability and path-analysis in landraces of bread wheat from southwestern Iran

Summary Nine pure lines of bread wheat collected from landraces of southwestern Iran and one local Iranian cultivar from the same area were used to estimate genetic variation and heritability for 12 developmental and yield component characters. Path-analysis was used to partition the genetic correlations between some of the characters into direct and indirect effects. Mean values for these lines were also compared with those of five improved cultivars from Iran and California. The goal of the experiment is to breed improved landraces and/or modern cultivars for areas of low input agriculture in southern California, southwestern Iran, and regions with similar environmental and agronomic conditions.Moderate genetic variation was displayed by the number of effective heads per plant, number of grains per head, and grain weight in the landrace genotypes. The heritability estimates ranged from 43 to 97%. Expected genetic advance with selection of the highest 5%, expressed as percent of the mean, was around 20% for number of heads per plant, number of grains per head, and 1000-grain weight.Days to booting, to heading, and to anthesis were positively correlated but none of them were significantly associated with days to maturity. Plant height had a negative genetic correlation with number of grains per head, 1000-grain weight, grain yield, and harvest index. The genetic correlation between number of heads per plant and number of grains per head, 1000-grain weight, and harvest index was also significantly negative. Harvest index had a negative genetic correlation with days to booting, to maturity, plant height, number of heads per plant, and straw yield and a positive correlation with number of grains per head, 1000-grain weight, and grain yield.Days to maturity, plant height, number of heads per plant, number of grains per head, 1000-grain weight, and harvest index each had a positive direct effect on grain yield. The first two characters exhibited the highest and lowest direct effects, respectively. The positive direct effects of days to maturity, plant height, and number of heads per plant, however, were partially or completely counter-balanced by their strong negative indirect effects through number of grains per head, 1000-grain weight, and harvest index. Pathanalysis indicated that late and tall landrace genotypes tend to produce more heads per plant, but with fewer number of grains per head, smaller grains, and lower harvest index.Comparisons between the local lines and the improved cultivars revealed that, in general, the former were much taller and produced a larger number of non-effective tillers. Mean number of grains per head, grain weight, harvest index, and grain yield of local lines were smaller than those of improved cultivars.Our observations indicate that the landraces could be improved by selecting for shorter genotypes with smaller numbers of tillers per plant, but with larger numbers of grains per head and heavier grains.     

长江中下游地区小麦产量育种的方向及其策略探讨

摘要：长江中下游地区近期育成的和大面积应用的小麦品种,在目前中高产水平下（400-450kg/666.7m2）,总体以千粒重对产量的作用较大,666.7平方米穗数对产量的作用也较大,但其与环境相关值较高,每穗粒数对产量的作用似较小,但其表型相关值较大;千粒重和666.7平方米穗数对产量的影响主要由本身的直接作用所至,而每穗粒数对产量的影响似主要被通过666.7平方米穗数和通过千粒重的间接作用所分解;不同产量水平下试验品种的三因素表现为,在相对产量由低中产到较高产量时,666.7 平方米穗数增加较多,每穗粒数变化较小,千粒重提高较多,而在高产组次中,666.7平方米穗数基本稳定（或略有下降）,每穗粒数增幅较大,千粒重急剧提高。至此,作者初步认为,本地区未来高产小麦育种,虽选育穗粒重齐头并进类型,或以大穗大粒为主类型,或穗粒并重类型似均有可行性,但根据本地区小麦生态条件的特点,在策略上宜稳定穗数,增加粒数,主攻（提高）粒重。同时,还探讨了相适宜的产量构成因素。     

冬小麦水旱品种间杂交主要性状的遗传分析

利用水地和旱地两种生态型的品种 ,采用 4× 4不完全双列杂交试验 ,对水旱品种间杂交主要性状的配合力和遗传表现进行了研究。结果表明 ,水地亲本的一般配合力效应对杂种后代主要性状的影响 ,除千粒重外 ,均达到显著或极显著水平 ;旱地亲本的一般配合力效应主要对株高、穗下节长、单株穗数、收获指数等与抗旱性有关的性状影响明显 ,且除株高和收获指数外 ,其余性状P1的方差均大于P2 的方差。说明水地亲本对杂种后代产量性状影响大 ,旱地亲本主要影响抗旱性状 ,水旱两种生态型杂交 ,实现了抗旱与丰产的有机结合。株高、穗下节长、穗粒数、单株产量、单株生物产量和收获指数以加性效应为主 ,而显性基因对单株穗数、千粒重、单穗重的影响较大。旱地小麦产量性状遗传力均较低 ,而株高、穗下节长、收获指数等与抗旱性有关的性状遗传力较高     

Evaluation of grain yield and its components in wheat cultivars and landraces under near optimal and drought conditions

In a 2-years experiment, 30 wheat cultivars and 21 landraces from different countries were tested under near optimum and drought stress conditions. Plant height, number of sterile spikelets per spike, spikelets per spike, number of kernels per spike, kernel weight per spike, 1000 kernel weight and grain yield were evaluated. The number of kernels per spike, 1000 kernel weight and especially yield were more sensitive to drought stress in the cultivars than plant height and number of spikelets per spike, while in the landraces these traits did not differ under drought stress compared to near optimum conditions. The average yield of cultivars was significantly better than the average yield of landraces under near optimum as well as drought stress conditions. Path coefficient analysis showed that for cultivars under near optimum conditions there was no significant direct association of any of the analysed characters with yield, while under drought stress conditions, number of kernels per spike had a significant positive direct effect. Under drought stress conditions, the number of sterile spikelets displayed a negative direct effect, while kernel weight per spike had a positive direct effect on yield. Hierarchical cluster analysis was used as a tool to classify cultivars and landraces according to their yield ability under near optimum and drought stress conditions. Among the cultivars, two groups out of five and among one of three in the landraces were characterised by high yields in both near optimum as well as under drought stress conditions. These genotypes may serve as sources of germplasm for breeding for drought tolerance. This revised version was published online in August 2006 with corrections to the Cover Date.     

冬小麦种质材料主要农艺性状研究

小麦种质资源是小麦育种的重要基础,小麦遗传多样性评价不仅有助于种质资源的搜集、管理和利用,也有利于核心种质的研究。为了合理利用小麦种质资源,降低组配小麦杂交组合的盲目性,选取73份国内外种质材料,在旱地条件种植,以株高、穗粒数、千粒重、有效穗数和产量为指标对其进行了聚类分析。结果表明,在雨养条件下各性状的变异系数大小为：产量>有效穗数>穗粒数>千粒重>株高;产量构成因素与产量的相关程度为：有效穗数>穗粒数>千粒重;供试材料可以分为六类,并对各类种质材料的特点及在育种中的应用作了评述。试验中综合表现较好的4个亲本材料为第Ⅵ组的18th FAWWON-IR-023、18th FAWWON-IR-111、18th FAWWON-IR-027和18th FAWWON-IR-169,可为选育抗旱优质丰产小麦新品种提供优异基因。     

水分胁迫对甜玉米主要农艺性状及产量的影响

在大棚内采用人工供水的方法,研究不同水分胁迫下甜玉米品种主要农艺性状与产量的关系。结果表明,轻度干旱胁迫下可用株高、茎粗、散粉至吐丝间隔期(ASI)、穗粗、每穗粒数、千粒重等6个性状耐旱系数作为品种耐旱性鉴定的指标,而中度干旱胁迫下可用穗位叶面积、散粉至吐丝间隔期(ASI)、穗粗、每穗粒数、出籽率、千粒重这6个性状耐旱系数预测品种产量的耐旱系数。在不同程度水分胁迫下,散粉至吐丝间隔期(ASI)、穗粗、每穗粒数、千粒重等4个性状耐旱系数是不同品种耐旱性鉴定的重要指标,除此之外,株高、茎粗、穗位叶面积等决定植株形态的性状指标也对甜玉米耐旱性起一定作用。     

不同矮秆基因小麦农艺性状的遗传差异

对含有不同主效矮秆基因（Ｒｈｔ１，Ｒｈｔ２和Ｒｈｔ１＋Ｒｈｔ２）的三组春小麦进行了两年，两地试验。结果表明，具有半矮秆基因Ｒｈｔ１的小麦表现出较高的生物产量，穗粒数和容重，它们以此获得较高的籽粒产量；具有矮秆基因Ｒｈｔ１和Ｒｈｔ２的小麦，则表现出较高的千粒重，通过半大收获指数获得高产；在两个半矮秆组内，籽粒产量与株高之间，粒数／ｍ＾２与千粒重之间均为显著负相关，就矮秆组而言，在籽粒产量与粒数／ｍ＾     

豫东平原冬小麦超高产栽培技术路线探讨

为了探讨豫东平原冬小麦超高产栽培技术路线,选用4个半冬性品种,划分为3个产量水平,进行产量结构变化情况分析、产量构成三要素与产量的回归及通径分析。结果表明：产量水平从9000~9750 kg/hm ²提高至9750~10500 kg/hm ²,种植密度、收获穗数、穗粒数、千粒重协调增加,有效穗数增幅稍大。穗数(x₁)、穗粒数(x₂)、千粒重(x₃)与产量(y)具有极显著的回归关系。通径分析表明对产量的贡献千粒重>穗数>穗粒数。因此,豫东平原冬小麦超高产栽培,应以提高千粒重为主攻方向,适当增加有效穗数,稳定穗粒数。从而提出豫东平原半冬性多穗型冬小麦超高产栽培“一提三增一稳五防”的技术路线。     

小麦超高产单株种子生产潜力及相关性状的研究

为探讨小麦单株种子生产潜力及相关性状特征,以57个不同基因型F2代小麦超高产单株为材料,对超高产单株产量性状、相关性状间的相关、通经和回归关系进行了分析。结果表明：（1）超高产单株（≥80 g/单株）田间选择概率为0.00518%;（2）株穗数、穗粒数、千粒重、结实小穗数、穗粒重和容重与单株产量呈显著正相关,不孕小穗数呈显著负相关,株高、穗长和千粒重相关不显著;（3）株穗数对单株产量的直接贡献最大,决定单株产量的65.33%,穗粒数、穗粒重、千粒重、株粒数分别决定单株产量的19.92%、18.15%、13.72%和5.6%。≥80 g/株的单株田间选择概率为0.00518%。     

矮秆超大穗小麦亲本与多抗丰产小麦主要农艺性状的配合力评价及遗传分析

为了进一步挖掘矮秆超大穗小麦亲本在遗传育种中的应用潜力,为小麦高产、超高产育种选择优良亲本和最优杂交组合提供理论依据,选用5个矮秆超大穗小麦品种（系）作为父本,8个多抗丰产小麦品种（系）作为母本,按NCII遗传交配设计,采用8×5不完全双列杂交法,配制了40个杂交组合,对小麦亲本及杂种F1的株高、主穗长、单株穗数、结实小穗数、主穗粒数、主穗产量、单株产量、千粒重8个性状进行了考察,并在基因型方差分析显著的基础上进行了配合力评价及遗传力分析。结果表明：父本中A1、A3、A4是综合性状优良的亲本,其多数性状的一般配合力均较高,特别是穗长、主穗粒数、结实小穗数、主穗产量、单株产量的一般配合力高,而株高的一般配合力效应值较低,是很理想的矮秆超大穗多粒亲本材料。母本中B5、B6单株穗数一般配合力较高,株高的一般配合力效应值较低,可作小麦高产杂交育种的矮秆多穗型亲本使用。结合特殊配合力效应分析：组合B5×A3单株穗数、主穗产量、单株产量、千粒重特殊配合力效应最高,株高特殊配合力效应较低,可作为的矮秆、多穗、粒重高且高产的重点杂交组合。B2×A4主穗长、单株产量、单株穗数特殊配合力效应都较高,而株高特殊配合力效应低,可作为矮秆大穗高产的重点杂交组合。遗传力分析表明：单株产量、千粒重、主穗长、主穗产量皆在70%以上,表明这4个性状的广义遗传力较大,受环境影响较小,而狭义遗传力的分析除主穗长、主穗粒数、主穗产量、株高大于50%,其余4个性状均低于50%,说明受环境影响大,不宜早期选择,应该适当推迟选择的代数。     

不同矮秆基因对冬小麦农艺性状的影响

利用以冬麦品种ＭａｒｉｓＨｕｎｔｓｍａｎ为背景的含有不同矮杆基因Ｒｈｔ１,Ｒｈｔ２和Ｒｈｔ３及其不同结合形式的６个近等基因系,研究了不同矮秆基因对小麦农艺性状的作用,结果表明,Ｒｈｔ１半矮秆基因显著提高了单株穗数,粒数和粒重,地下部生物产量,经济系数和倒二叶面积,Ｒｈｔ２半矮秆基因显著提高了单株（或单穗）粒数和粒重,经济系数和倒二叶面积,显著降低了千粒重,Ｒｈｔ３矮秆基因对单株粒数,地上部生物产量     

The Effect of Late-terminal Drought Stress on Yield Components of Four Barley Cultivars

Barley (Hordeum vulgare L.) is an important winter cereal crop grown in the semiarid Mediterranean, where late‐terminal drought stress during grain filling has recently become more common. The objectives of this study were to investigate the growth performance and grain yield of four barley cultivars under late‐terminal drought stress under both glasshouse and field conditions. At grain filling, four barley cultivars (Rum, ACSAD176, Athroh and Yarmouk) were exposed to three watering treatments: (1) well‐watered [soil maintained at 75 % field capacity (FC)], (2) mild drought stress at 50 % FC, (3) severe drought stress at 25 % FC in the glasshouse experiment and (1) well‐watered (irrigated once a week), (2) mild drought (irrigated once every 2 weeks), (3) severe drought (non‐irrigated; rainfed) in the field. As drought stress severity increased, gross photosynthetic rate, water potential, plant height, grain filling duration, spike number per plant, grain number per spike, 1000‐grain weight, straw yield, grain yield and harvest index decreased. In the glasshouse experiment, the six‐row barley cultivars (Rum, ACSAD176, and Athroh) had higher grain yield than the two‐row barley cultivar (Yarmouk), but the difference was not significant among the six‐row cultivars under all treatments. In the field experiment, Rum had the highest grain yield among all cultivars under the mild drought stress treatment. The two‐row cultivar (Yarmouk) had the lowest grain yield. In general, the traditional cultivar Rum had either similar or higher grain yield than the other three cultivars under all treatments. However, the yield response to drought differed between the cultivars. Those, Rum and ACSAD176, that were capable of maintaining a higher proportion of their spikes and grains per spike during drought also maintained a higher proportion of their yield compared with those in well‐watered treatment. In conclusion, cultivar differences in grain yield were related to spike number per plant and grain number per spike, but not days to heading or grain filling duration.     

小麦收获指数与主要农艺性状的相关性探析?

小麦收获指数是指籽粒产量占地上部生物产量的百分率。研究小麦品种主要农艺性状和经济性状间与其产量的相互关系,采用相关性分析与回归方法,对影响收获指数的10个主要农艺性状及因素进行了研究分析。结果表明：供试小麦HI具有较大的改良空间;HI与穗颈长、株高、穗长、小穗数、主茎生物产量有显著负相关,与单穗质量、产量呈正相关,与生物产量没有显著相关性;主成分分析结果显示,3个主成分累积贡献率达 87.427%,表明 3 个主成分已覆盖所有性状的主要信息;通过回归分析,小麦HI与单穗籽粒产量、生物产量、千粒质量有显著回归关系。结论: 通过对10份小麦材料农艺性状的统计分析,得出如下结论。HI受单穗质量、生物产量、千粒重影响较为明显有显著回归关系,与单穗质量、产量和穗粒数有明显正效应,生物产量对其有负效应。小麦HI提高可通过选育生物产量不宜过大,而穗粒数较高、单穗重较大的小麦品种。     

冬小麦品种选育若干问题探讨

为提高育种预见性和选择效率,对小麦株型与冠层、株高、单位面积穗数等几个选择指标进行了探讨。认为株高80～90cm,旗叶较宽、较短,面积较小,挺而上冲,旗叶、倒二叶、倒三叶的长度依次加长,叶基角和开张角依次加大。株型从生长前期到生长后期由紧凑到松散有一定动态变化的中间型品种更易实现高产稳产;现代品种要在保持较高收获指数的基础上,注重生物产量、单位面积粒数、穗粒数／旗叶面积、穗粒重／旗叶面积的选择。     

播期播量对不同穗型冬小麦群体及子粒产量的影响

为明确不同播期播量处理对两种穗型小麦群体结构和产量构成要素的影响,以大穗型品种山农23号和多穗型品种济麦22为材料,研究了小麦群体动态、花后叶面积指数、子粒灌浆进程及产量构成要素的变化规律。结果表明,10月10日播期处理有利于两个品种小麦群体的构建,延迟播期后,即使增大播量也不能获得高产。山农23号在延迟播期后,有效叶面积减少,子粒灌浆速率减慢,单位面积穗数和穗粒数降低,差异显著,加大播量后,穗粒数减少,千粒重降低;济麦22延迟播期后单位面积穗数和千粒重下降,加大播量后,单位面积穗数不升反降,花后叶片衰老加剧。本试验条件下,两个品种的最适播期均为10月10日,山农23号的适宜播量为180kg/hm ²,济麦22的适宜播量为105kg/hm ²。     

Analysis of Wheat Cultivar Differences in Grain Yield, Grain Nitrogen Yield and Nitrogen Utilization Efficiency.

More detailed information on the causes of yield variability among wheat cultivars is needed to further increase wheat yield. Field studies were conducted in Northern Greece over the two cropping seasons of 1985—1986 and 1986—1987 to assess the effects of nitrogen fertilizer and application timing of the various component traits that determine grain yield, grain nitrogen yield and nitrogen utilization efficiency of two bread ( Triticum aestivum L.) and two durum ( Triticum durum Desf.) wheat cultivars, using yield and yield component analysis. Nitrogen at a rate of 150 kg ha^-1 was applied before planting or 100 N kg ha^-1 before planting and then 50 N kg ha^-1 top dressed at early boot stage. Nitrogen and cultivars affected all traits examined, while split nitrogen application affected only some of the traits. Grain yields in the most cases were correlated with number of grains per unit area and grain weight and grain nitrogen yields in all cases with grain number per unit area. The contribution of the number of grains per spike to total variation in grain yield among cultivars was almost consistent (37 to 55 %), while the contribution of grain weight was more significant (up to 55 %) in high yields (>6.500kg ha^-1) and number of spikes per unit area (>500). The number of grains per spike contributed from 60 to 83 % to the total variation in grain nitrogen per spike. Increased grain nitrogen concentration resulted in a reduction of its contribution in grain nitrogen yield variation. Nitrogen utilization efficiency was higher during grain filling than during vegetative biomass accumulation. The contribution of nitrogen harvest index to the variation of utilization efficiency for grain yield was higher in plants receiving nitrogen application.     

大麦种质资源成株期抗旱性鉴定及抗旱指标筛选

干旱是影响大麦生产的主要因素之一。在鉴定大麦种质资源成株期抗旱性的基础上,筛选抗旱指标,可为培育抗旱品种提供依据。本研究在2016和2017年在大麦生长主要需水期平均降雨量不足40 mm的甘肃省武威市进行田间试验,以30份大麦种质资源为研究对象,设置正常灌水和干旱胁迫处理,测定大麦株高、穗长、分蘖数、单株粒数、单株粒重、穗粒数、千粒重和产量,采用抗旱性度量值(D)、综合抗旱系数(CDC)、加权抗旱系数(WDC)、相关分析、频次分析、主成分分析、灰色关联度分析、隶属函数分析、聚类分析和逐步回归分析相结合的办法,对其进行抗旱性鉴定及抗旱指标的筛选。干旱胁迫对测定的各指标均有极显著影响。频次分析表明,各指标对干旱胁迫反应的敏感程度依次为产量、株高、单株粒重、穗长、单株粒数、分蘖数、穗粒数和千粒重。相关分析表明,产量与株高、穗长、分蘖数、单株粒数和单株粒重呈极显著正相关,与穗粒数呈显著正相关、与千粒重呈不显著正相关。主成分分析表明,5个主成分可以代表大麦抗旱性86.39%的原始数据信息量。基于D值、CDC值和WDC值的大麦种质资源抗旱性排序基本一致。灰色关联度分析表明,各指标单项抗旱系数值与D值间的关联度依次为产量、单株粒重、单株粒数、穗长、株高、分蘖、穗粒数和千粒重,各指标DC值与WDC值间的关联大小依次为单株粒重、产量、单株粒数、分蘖、穗长、穗粒数、株高和千粒重。根据D值进行聚类分析,将供试大麦种质资源依据抗旱性从强到弱分为5个抗旱等级,其中1级5份、2级1份、3级11份、4级10份、5级3份。除分蘖和千粒重外,其余指标的隶属函数值、CDC值、D值和WDC值均随着抗旱级别的升高而增大。回归分析表明, 5个测定指标均与D值密切相关。干旱胁迫对大麦种质资源成株期各指标均有极显著影响。确定了D值为适宜的抗旱鉴定指标。筛选出成株期抗旱性强的大麦种质资源甘啤7号、Z06-278-9、MERIT、NEVADA和西藏25,可为大麦抗旱育种、抗旱机制以及干旱调控缓解机制的研究提供技术支持。穗长、单株粒数、单株粒重、穗粒数、产量可作为大麦种质资源成株期简单、直观的抗旱评价指标。     

早籼稻品种更替过程中农艺性状的演变特征

以南方稻区品种更替过程中大面积推广的18个早籼稻代表品种为材料,按高秆品种、矮秆品种、矮抗品种、优超品种4个演变阶段,采用常规栽培方法研究其农艺性状的演变特征。结果表明,随着品种的演变,产量、收获指数、每穗粒数均有提高;有效穗、结实率、千粒重变化不大。进一步分析表明,每穗粒数的增加是着粒密度改良所致。农艺性状与年代的回归分析表明,生物量与株高在1980年后有增加的趋势,生育期有缩短的趋势。相关分析表明,决定早籼稻产量的农艺性状为生物量、株高、收获指数和每穗粒数。     

喀斯特山区野生燕麦农艺性状的主成分与灰色关联度分析

为进一步发掘喀斯特山区野生燕麦的优异基因资源,拓宽燕麦资源的遗传基础,对采自不同喀斯特山区的80份野生燕麦进行了农艺性状进行主成分和灰色关联度分析。结果表明,主成分分析中,筛选出的特征根累积贡献率大于85%的有穗粒数因子、穗长因子、株高因子以及千粒重因子,共4个主成分;灰色关联度分析中,株高与穗长、有效穗与穗粒数、小穗与株高、穗粒重与穗粒数、千粒重与穗粒重之间相互的关联度最大,关系最为密切。     

30余年来北部冬麦区小麦品种产量改良遗传进展

采用1965年以来北部冬麦区育成的10个主栽品种,在水、肥供应充足、病、虫、倒伏得到有效控制条件下,连续两年共进行4个点次试验;同时对1B/1R易位系和矮秆基因进行分子鉴定。结果表明,品种产量年遗传进展为64.63 kg hm^-2 或1.20%,其较大幅度的提高出现在1980年丰抗号品种育成时期。丰抗2号和丰抗8号是该地区最早育成的含有1B/1R染色体和Rht-D1b矮秆基因的品种。主要农艺性状演变的总趋势是抽穗期提前、株高显著降低、生物学产量基本保持稳定、单位面积穗数减少、每穗粒数有所增加、千粒重和收获指数大幅度提高,其年遗传进展分别为－0.1%、－0.70%、0.35%、－0.50%、0.63%、1.12%和0.79%。通过减少不孕小穗和小花数来增加每穗粒数,通过提高灌浆速率来提高千粒重,再把高穗粒重与较多的单位面积穗数结合在一起,是北部冬麦区进一步提高产量的重要途径。