利用多时相Sentinel-1 SAR数据反演农田地表土壤水分

土壤水分是陆面生态系统水分和能量循环的重要变量,在农田干旱监测、作物长势监测和作物估产等应用研究中具有重要的作用。该文结合基于变化检测的Alpha近似模型,利用Sentinel-1卫星获取的多时相C波段合成孔径雷达(synthetic aperture radar,SAR)数据,实现了农田地表土壤水分的反演。该文首先利用微波辐射传输模型验证了Alpha近似模型在土壤水分反演中的合理性。研究发现,对于土壤散射占主导的区域,Alpha近似模型对辐射传输模型有较好的近似,能够有效地消除地表粗糙度和植被对雷达后向散射系数的影响。在此基础上,结合怀来研究区多时相Sentinel-1 SAR数据,利用Alpha近似模型构建了土壤水分观测方程组,通过求解方程组得到了农田地表土壤水分。地面验证结果表明,土壤水分反演的均方根误差(root mean square error,RMSE)为0.06 cm3/cm3,平均偏差为0.01 cm3/cm3,精度较好。该文研究为利用高重访周期、多时相的Sentinel-1 SAR数据获取农田地表土壤水分提供了参考。     

双极化雷达反演裸露地表土壤水分

为了快速高效地获取大面积地表土壤水分,本文提出一种适用于双极化SAR(synthetic aperture rader)的裸露地表土壤水分反演经验模型。首先通过AIEM(advanced integral equation model)模型数值模拟和回归分析,提出一种新的粗糙度参数,将2个传统的粗糙度参数简化为1个参数;然后模拟地表土壤水分与雷达后向散射系数的关系,从而建立裸露地表的经验散射模型,模型的未知参数仅为粗糙度参数和土壤体积含水量,通过双极化的雷达数据即可实现土壤水分的反演。通过2008年甘肃张掖黑河流域实测数据对模型进行了初步验证,发现在入射角大于25°时,模型反演值与实测值有着良好的相关关系(相关系数为0.745)。该模型仅需双极化的雷达数据就能实现土壤水分的反演,无需测量地面粗糙度,尤其适用于大面积干旱区域的地表土壤水分的获取。     

光学与微波遥感协同反演藏北表层土壤水分研究

表层土壤水分是定量干旱监测的重要参量,对干旱区生态环境具有十分重要的意义。在采用归一化植被指数阈值法划分地表覆盖类型的基础上,利用MODIS数据选择适用的光学遥感算法估算土壤水分基准值,以及利用风云三号B星搭载的微波成像仪(Fengyun-3B/MicrowareRadiationImagery,FY3B/MWRI)数据采用微波遥感算法反演土壤水分日变化量,最后构建藏北表层土壤水分协同反演的遥感模型并应用于区域土壤水分的估算。结果表明:光学遥感与微波遥感协同反演的土壤水分含量与实测数据呈显著相关,决定系数达到0.89,均方根误差为0.97,协同反演模型具有较高的反演精度,并且协同反演的结果优于单一遥感源的反演结果。该模型可以较好地适用于藏北地区表层土壤水分的动态监测。     

基于Sentinel多源数据的农田地表土壤水分反演模型构建与验证

土壤水分是影响水文、生态和气候等环境过程的重要参数,而微波遥感是农田地表土壤水分测量的重要手段之一。针对微波遥感反演农田地表土壤水分受植被覆盖影响较大的问题,该文基于Sentinel-1和Sentinel-2多源遥感数据,利用Oh模型、支持向量回归(support vector regression,SVR)和广义神经网络(generalized regression neural Network,GRNN)模型对土壤水分进行定量反演,以减小植被影响,提高反演精度。结果表明:通过水云模型去除植被影响后的Oh模型反演精度有所提高。加入不同植被指数的SVR和GRNN模型的反演效果总体优于Oh模型,基于SVR模型的多特征参数组合(双极化雷达后向散射系数、海拔高度、局部入射角、修改型土壤调整植被指数)反演效果最优,其测试集相关系数和均方根误差分别达到了0.903和0.015 cm~3/cm~3,为利用多源遥感数据反演农田地表土壤水分提供了参考。     

CCI多传感器组合土壤水分产品在青藏高原不同地区的适用性

青藏高原的土壤水分通过对水循环和对生态系统的作用,对区域乃至全球气候的变化起着重要的影响。由于该地区缺失长期和大规模土壤水分的现场观测,遥感产品成为地球系统模型的有用数据集。其中,欧洲航天局(European Space Agency, ESA)发布的气候变化倡议(Climate Change Initiative, CCI)土壤水分产品已在全球范围内广泛应用。研究使用3个网络的原位测量土壤水分数据评估CCI(主动、被动、主被动组合)产品在青藏高原地区的适用性,这3个网络分别代表青藏高原的半湿润(玛曲)、半干旱(那曲)和干旱(狮泉河)气候条件。结果表明,CCI3种产品都能捕捉到青藏高原生长季土壤水分时间变化规律和东南高西北低的空间分布特征。其中主被动组合产品在3个网络的相关性都是最高的。在以高寒草甸为主的半干旱地区,CCI主被动组合产品反演土壤水分具有较高的精度,与实测数据相关系数高达0.870,均方根误差小于0.06,但在高密度植被区有所低估,裸露地表有所高估。从空间分布来看,主动产品过高估计了青藏高原土壤水分,被动产品显示土壤水分空间分布变化范围过大,最大值达0.98 m~3/m~3,主被动组合产品在空间上(0～0.65 m~3/m~3)最接近青藏高原土壤水分实际分布情况。研究结果可以为CCI产品在青藏高原的应用与研究提供参考。     

基于AMSR-E与MODIS数据的新疆土壤水分协同反演与验证

针对当前遥感在大范围土壤水分估算中面临的问题,提出将被动微波遥感数据与光学/热红外遥感数据在模型中协同反演陆表土壤水分的新方法:利用MODIS的光学与热红外波段反演土壤水分的基准值;利用AMSR-E传感器的X波段反演土壤水分的日变化量,然后集成二者建立土壤水分协同反演模型。以新疆为实验区,采用在典型地区获取的365个土壤水分实测值,对该模型进行了验证与精度分析。结果表明,协同反演模型的估算结果与地面实测值之间有着更好的相关性和较小的均方根误差,明显优于单一数据源或单一模型的反演结果。     

基于L波段的裸土区土壤水分微波遥感反演研究

以北京市大兴区为研究区,探讨利用ALOS/PALSAR数据反演裸土土壤水分的方法。由于PALSAR的后向散射系数主要与土壤水分及地表粗糙度有关,本研究使用AIEM理论模型计算地表的同极化后向散射系数,Oh半经验模型计算交叉极化的后向散射系数;由分析可知,同极化与交叉极化的差异不随土壤水分的变化而变化,仅随地表粗糙度的增加而减少,为此可建立后向散射系数与粗糙度之间的函数关系。本文利用BP神经网络算法反演研究区的裸土土壤水分含量,并利用实测数据对反演结果进行验证,结果表明估测裸土土壤水分含量误差为0.035 m3/m3,相对误差为13.9%。因此,可以利用L波段主动微波遥感反演裸土土壤水分含量,且具有较高的精度。本研究成果可为农业灌溉、灾害监测、环境评估等提供信息支持,具有重要的现实意义与应用价值。     

基于改进粒子群神经网络的麦田土壤水分反演

作物区土壤水分遥感反演往往受到作物冠层、地表粗糙度等因素影响,较难得到满意的土壤水分反演结果。为解决麦田土壤水分反演的问题,提出一种改进粒子群神经网络优化算法。该算法将多源光学和雷达影像数据进行联合,利用改进粒子群算法对神经网络权值进行优化,建立遥感影像与土壤水分之间一种隐式映射,并将影像数据作为该优化模型的输入变量,对麦田土壤水分进行反演。结果表明改进粒子群神经网络优化算法反演精度高于其他的反演方法,同时主被动遥感联合反演地表土壤水分的精度也比仅使用单一数据源作为输入的方法精度高,R~2达到0.807,RMSE为0.043 cm~3cm~(-3)。由此可见,改进粒子群神经网络的优化方法是可行的,可以有效建立后向散射系数与土壤水分之间的隐式关系,获取较高精度的土壤水分值。该方法可为利用雷达影像数据进行大范围土壤水分估测提供支持。     

基于AMSR-2蒙古高原土壤水分反演及 对气象因子响应分析

土壤水分是地表水文过程研究的一个重要参数,是众多环境因子综合作用的结果,科学判定土壤水分对环境因子的响应特性,对在蒙古高原地区开展干旱监测预警,调整农业生产结构,改善区域生态环境具有重要意义。本研究基于AMSR-2观测亮温、SPOT-NDVI数据,利用微波辐射传输模型及粗糙地表发射率Qp模型,构建适合蒙古高原的土壤水分反演方程,同时将模型应用于2013年蒙古高原植被生长期土壤水分反演。在此基础上,结合TRMM 3B43降雨量及气象站点气温数据,探讨了蒙古高原土壤水分对气象因子及植被的响应特性。结果表明:1)构建的蒙古高原表层土壤水分反演模型精度较高,土壤水分反演值与实测值的判定系数为0.680 6,均方根误差(RMSE)达0.031 6 cm3·cm-3,反演结果明显优于JAXA提供的AMSR-2土壤水分产品数据(RMSE=0.044 1 cm3·cm-3)。2)TRMM 3B43降雨数据与实测降雨量线性拟合,其判定系数为0.859 8,直线拟合斜率K=0.941 5,在数值上较站点实测值略微偏低,表明TRMM 3B43数据精度较高,在蒙古高原具有很好的适用性。3)蒙古高原植被生长期土壤水分、植被指数及降水量在空间格局上均表现出由北向南、由东北向西南逐渐减少的趋势。干旱区,土壤水分对气温变化最敏感,二者表现出显著正相关关系,其次为降水和植被;半干旱区,植被是影响土壤水分的关键因子,而气温与降水对土壤水分影响呈现出季节性变化;半湿润区3个因子对土壤水分的影响程度表现为植被降水气温。总之,利用土壤水分对气象因子和植被的响应特性,可以采取适当措施降低蒙古高原灾害发生风险,为区域生态环境建设提供科学依据。     

水分对苹果园土壤呼吸季节性与年际变化的影响

[目的]了解果园土壤呼吸的季节和年际变化及其影响因素,为退耕还果条件下黄土高原地区土壤碳源汇功能变化研究提供依据。[方法]在长武农田生态系统国家野外站,以盛产期果园为对象,利用土壤碳通量监测系统(Li-COR,Lincoln,NE,USA)连续3 a原位监测了土壤呼吸、土壤水分和温度变化,分析了土壤呼吸的季节性和年际间的变化及其与水分、温度变化之间的关系。[结果]土壤呼吸具有明显的季节和年际变异特征:最高值出现在雨季(7—9月),3 a分别为3.14,3.98,4.71μmol/(m~2·s),最低值出现在11月后,3 a依次为0.99,0.88,0.69μmol/(m~2·s);年际间累积呼吸量变异约21%。土壤呼吸与温度呈显著指数关系,而不同水分状况下土壤呼吸及温度敏感性(Q_(10))不同,当土壤水分含量11.12%时,土壤呼吸为2.01μmol/(m~2·s),当土壤含水量变化于11.12%～23.63%之间时,土壤呼吸为2.24μmol/(m~2·s),当土壤含水量23.63%时,土壤呼吸则为1.38μmol/(m~2·s);相应地不同水分条件下Q_(10)值分别为1.57,1.63和1.38。[结论]土壤水分显著影响黄土区苹果园土壤呼吸和Q_(10),研究结果为黄土区果园生态系统碳汇功能的估算提供了依据。     

Soil-profile distribution of primary and secondary plant-available nutrients under conventional and no tillage

Nutrient distributions under no tillage (NT) compared with conventional disk-and-bed tillage (CT) management in the warm, humid region of the southeastern USA need to be assessed so that future placement, quantity, and type of fertilizers can be altered, if necessary, to efficiently match crop demands. We determined soil-profile distributions of pH, N, P, S, K, Ca, Mg, Na, Zn, Fe, Mn, and Cu to a depth of 0.9 m at the end of 8.5 years of continuous CT and NT management on a Weswood silty clay loam (fine, mixed, thermic Fluventic Ustochrept) in southcentral Texas. Most dramatic changes occurred within the 0–0.05 m depth, where soil under NT had lower pH, Fe, and Cu than under CT, but greater P, K, Zn, and Mn. Greater P and K under NT than under CT also occurred below the till-zone (0.15–0.3 m). At a depth of 0–0.3 m, soil under NT contained greater amounts of extractable P, K, Zn, Fe, Mn, and Cu than under CT. Nitrogen fertilization had little effect on nutrient distributions, except resulting in greater extractable K at 0–0.05 m and greater nitrate at 0–0.15 m. Few changes in soil-profile distributions were observed for extractable S, Ca, Mg, and Na. Long-term continuous use of NT on this fine-textured, high-fertility (except for N) soil had no apparent adverse effects on nutrient distributions relative to CT, but enhanced conservation and availability of P, K, Zn, Fe, Mn, and Cu near the soil surface where crop roots proliferate.     

Assessing the effectiveness of conservation measures: Resolving the “wicked” problem of the Steller sea lion

“Wicked” problems are those that are complex and that change when solutions are applied. Many conflicts in conservation fall into this category. The study approached the problem of how to constrain the apparent wickedness of a problem in the conservation management of a species by using simple empirical indicators to carry out iterative assessment of the risk to a population and to document how this risk evolves in relation to the addition of new data and the implementation of management actions. Effects of high levels of uncertainty within data and also concerning population structure were examined through stochastic simulation and by exploration of scenarios. Historical trends in the example used, the Steller sea lion, showed rapid declines in abundance in some regions during the 1980s. The current total population is 130,000-150,000 Steller sea lions through Alaska and British Columbia and this number has been stable since about 1990 in spite of regional differences in population dynamics. Regional differences in the sequence of changes in the number of pups and non-pups, suggested that an internal re-distribution of juveniles could have happened between 1980 and 1990. Current productivity also appears close to the long-term mean. Stochastic population projection using various scenarios showed that, based upon this history, the risk of extinction for the population has declined and is below reasonable thresholds for considering the population to be endangered. The trends in risk suggest that management actions taken since 1990 have probably been effective. Consequently, the conservation management objectives for the Steller sea lion are probably being met. The approach provides a mechanism, based upon experience and scenario analysis, for exploring future policy options and may help to constrain the debate amongst stakeholders about the cost-benefit trade-offs associated with different options.     

“绿色革命”以来大气CO2浓度升高对C3作物营养品质的影响

自20世纪60年代"绿色革命"以来,育种技术和农耕技术的发展促进了农作物产量的大幅提升,然而作物的营养品质出现下降趋势。在相似的遗传背景下,大气CO_2浓度升高会使单位体积农作物产品的营养元素含量下降,因此"绿色革命"至今,农作物产品的营养元素下降可能受大气CO_2浓度升高影响。通过植物生长箱模拟"绿色革命"初期和目前的大气CO_2浓度水平(310μmol/mol和400μmol/mol),针对主要C_3作物水稻、小麦和大豆,研究"绿色革命"以来大气CO_2浓度升高对其籽粒的C、N、Fe、Zn元素含量的影响,结果表明:CO_2浓度升高对3种作物籽粒的C元素含量几乎没有影响,变化幅度在±1.5%之间;籽粒的N、Fe、Zn元素含量普遍呈现下降趋势,但均未达到显著水平。     

Potential of wild species for genetic enhancement of some semi-arid food crops

Discovery and incorporation of genes from wild species provide means to sustain crop improvement, particularly when levels of resistance in the cultigens are low and virulent strains of pests and pathogens overcome the host plant resistance. The extent of utilization and the potential of the wild genepool for genetic enhancement were reviewed in five important food crops viz. sorghum, pearl millet, chickpea, pigeonpea and groundnut grown in the semi-arid tropics. Introgression from compatible wild germplasm in the primary gene pool resulted in transfer of new cytoplasmic male sterility systems in pearl millet and pigeonpea, development of high protein, cleistogamous flower and dwarf pigeonpea lines and foliar disease resistant groundnut cultivars. Utilization of wild species in secondary and tertiary gene pools has been generally limited due to sterility, restricted recombination or cross incompatibility. Nevertheless, these species are extremely important as they contain high levels of resistance to several important biotic and abiotic stresses. Several of them, like those belonging to the Parasorghum section and the rhizomatous Arachis species are sources of multiple resistances and hold great promise to sustain crop productivity.     

Enhanced establishment and growth of giant cardon cactus in an eroded field in the Sonoran Desert using native legume trees as nurse plants aided by plant growth-promoting microorganisms and compost

To evaluate the feasibility of long-term desert reforestation technology of mixed vegetation, cardon cactus (Pachycereus pringlei) seedlings from indoor and outdoor nurseries were planted in the field adjacent to one seedling of potential legume nurse trees: mesquite amargo (Prosopis articulata), yellow palo verde (Parkinsonia microphylla), and blue palo verde (Parkinsonia florida). Some of the planting holes were also supplemented with common dairy compost. Additionally, the combinations of legume tree–cactus were inoculated with either a consortium of desert arbuscular mycorrhizal (AM) fungi, plant growth promoting bacteria (PGPB; the diazotroph Azospirillum brasilense Cd, and the phosphate solubilizer Paenibacillus sp.), or a mixture of all. The field experiments were evaluated periodically during 30 months for survival and growth. Cardons reared in an outdoor screen house survived better in the field than those reared in a controlled growth chamber and hardened later outdoors. Association with any legume nurse tree increased survival and enhanced growth of untreated cardons. For cardons growing alone, application of either compost, AM fungi, and all the treatments combined increased survival. For these plants, no treatment affected plant growth during the first 3 months after transplanting. Later, all treatments, except for AM fungi, enhanced plant growth. However, only 2 years after transplanting the enhanced growth effect of AM fungi was also significant. In the presence of the legume nurse trees, transient positive effects on cardon growth were recorded. General evaluation after 30 months of cultivation showed that the treatments positively affected cardon growth when growing alone or in combination only with mesquite amargo but not with the other two legume trees. This study proposes that young legume trees have the capacity to enhance survival and growth of cardon cactus, depending on the legume cactus combination. Additional treatments such as compost or PGPB can either amplify the effect or else attenuate it.     

Karyotypic and genome size variation in Cajanus cajan (L.) Millsp. (pigeonpea) and some wild relatives

Detailed karyotypes and 4C DNA amounts have been studied in five cultivars of Cajanus cajan and 20 species belonging to Cajanus, Rhynchosia, Dunbaria, Flemingia and Paracalyx. C. cajan shows intraspecific variability and its karyotype is most similar to that of C. cajanifolius (sect. Cajanus) in the morphology and number of satellite chromosomes and the lack of any correlation between chromosome size within the complement and asymmetry. Karyotypes of C. lineatus and C. sericeus belonging to sect. Atylia are similar with respect to maximum r-index and the ratio of longest and shortest chromosomes in their respective complements. C. acutifolius (sect. Frutocosa) is distinct in having a very low ratio (1.44) between the longest and the shortest chromosomes, while in C. albicans, C. goensis, C. scarabaeoides (sect. Cantharospermum) there is no chromosome pair with r-index >2.0. C. mollis and C. volubilis (sect. Volubilis) show similarity with regard to ratio between the longest and the shortest chromosomes and a distinct chromosome pair with an arm ratio of 2.7 and C. platycarpus is distinct (sect. Rhynchosoides) in having the smallest ratio (1.36) between the longest and the shortest chromosomes. 4C DNA amounts in Cajanus and Rhynchosia vary between 3.28 pg to 11.69 pg and 4.92 pg to 11.13 pg respectively, while in Dunbaria, Flemingia and Paracalyx these vary between 4.70 and 7:06 pg. In the genus Cajanus karyotypic features and 4C DNA amounts agree with the sectional classification and bring out a close relationship between C. cajan and C. cajanifolius. This is supported by studies based on seed protein patterns, isozyme analysis, trypsin and chymotrypsin inhibitor patterns, RFLP and RAPD data and crossability relationships. A clear relation between DNA amount and abnormalities effecting the extent of pairing and recombination in interspecific hybrids shows the importance of this study in developing future breeding programme involving C. cajan and its wild relatives that are of potential value.     

Faculty positions:Center for Agricultural Resources Research,Chinese Academy of Sciences

正The Center for Agricultural Resources Research(CARR),the Institute of Genetics and Developmental Biology(IGDB),Chinese Academy of Sciences,invites applicants for several research group leader positions.CARR is one of the research organizations in Chinese Academy of Sciences(CAS).We seek nominations and applications     

Effect of some fungicide seed treatments on the survival of Bradyrhizobium japonicum and on the nodulation and yield of soybean [Glycine max. (L) Merr.]

Several commercial fungicide seed treatments were evaluated for their possible effect on the survival of Bradyrhizobium japonicum on seeds and on the nodulation and yield of soybeans in a greenhouse and a field experiment. quinolate Pro (carbendazim and oxine copper), Vitavax 200FF (carboxin and thiram), and Monceren (pencycuron) had a small effect or no effect on the survival of B. japonicum and on the nodulation and yield of soybeans. They can thus be considered compatible with soybean seed inoculation. Germipro UFB (carbendazim and iprodione), Apron 35J (metalaxyl), and Tachigaren (hymexazol) decreased B. japonicum survival and the nodulation and yield of soybeans and thus cannot be considered compatible with soybean seed inoculation.     

Einfluss der düngungsmethoden auf die entwicklung von zuckerrübenkrankheiten

Während neunjähriger (1994-2002) Untersuchungen in der Versuchsanstalt Ba?cyny (Fahlerde, Fall-Gleyboden, ausgebildet aus leichtem Lehmboden) wurde der Befall mit Krankheiten an der Zuckerrübe in Abhängigkeit von der Düngungsmethode bewertet. Folgende Methoden wurden angewendet: A - Mineraldünger, B - Stalldung und Mineraldünger, C - Stroh-, Gründung und Mineraldünger, D - Kompostdünger und Biopräparate (ökologische Düngung). Auf den Objekten A, B, C wurden bei konstanter Phosphor-Kalium-Düngung die Stickstoffgaben von 0 bis 180 kg.ha^?1 differenziert. Auf dem ökologischen Objekt D ohne Mineraldünger wurden statt Stickstoff die Biopräparate P₅₀₀ und P₅₀₁ verabreicht. Der geringste Befall von Krankheiten wurde an der Zuckerrübe auf jenem Objekt festgestellt, das mit Kompost und den Biopräparaten gedüngt worden war. Im Rahmen der konventionellen Düngungsmethoden wurde allgemein die höchste Intensität der Krankheiten an der mit Stroh, Gründung und Mineraldünger gedüngten Zuckerrübe beobachtet. Ausnahmen von dieser Regel bildete das Vorkommen von echtem Mehltau und Keimlingsfäule. Auf den Objekten A, B, C nahm die Intensität der Krankheiten mit der Steigerung der Stickstoffgabe zu. Auch die auf Objekt D verabreichten Biopräparate stimulierten die Entwicklung der Krankheiten. The effect of various fertilization methods on the diseases of sugar beet was estimated on the basis of nine-year studies (1994-2002) conducted in the Experimental Production Farm in Ba?cyny on loess-based, sedimentary-gley, light-clay soil. The following fertilization methods were applied: A - mineral fertilization, B - mineral and manure fertilization, C - straw, green manure and mineral fertilization, D - ecological compost and biodynamic preparations fertilization. In the objects A, B and C fertilized with phosphorus and potassium, various doses of nitrogen ranging from 0 to 180 kg/ha^?1 were used. In the ecological object D, where mineral fertilization was not used, biodynamic preparations P₅₀₀ and P₅₀₁ were applied instead of nitrogen. The lowest ratio of the diseases of sugar beet was observed in the object fertilized with the compost and biodynamic preparations. In view of the conventional fertilization methods, generally the highest incidence of diseases was noted in the sugar beet fertilized with straw, green manure and mineral fertilizers. The exception was the occurrence of powdery mildew and the black leg. In objects A, B and C the incidence of the diseases increased with the increase in nitrogen doses. The applied biodynamical preparations in the object D stimulated the development of diseases too.     

Prospects for control of Pythium damping-off of lettuce with Trichoderma,Gliocladium and Enterobacter spp.

Summary Damping-off of lettuce (Lactuca sativa) caused by Pythium ultimum was studied in pots containing a non-sterile potting mix in the glasshouse. Fifty P. ultimum sporangia g^-1 compost reduced the plant stand to 15% and shoot dry weight to 18%, but this reduction was totally prevented by applying Trichoderma harzianum at 2×10⁵ viable propagules g^-1 potting mix. Gliocladium virens and Enterobacter cloacae also alleviated the damping-off. E. cloacae did not adversely affect the action of the fungal antagonists. The co-existence of the bacterial and fungal antagonists was revealed on the root surface and inner surface of the testa by scanning electron microscopy, indicating the compatibility of the biocontrol agents.