长期施肥对旱地红壤微团聚体磷素有效性的影响

【目的】土壤微团聚体是磷素储存与周转的重要载体,明确长期施肥下旱地红壤微团聚体中赋存磷素的形态及其生物有效性,为磷肥的高效合理施用提供理论依据。【方法】以位于鹰潭农田生态系统国家野外科学观测研究站院内的长期肥料定位试验(1988～2014)为依托,分别以有机无机配施区的1/2 NPK(CK)、低量NPK+稻秆(RS)与低量NPK+猪厩肥(PM)处理及无机肥区的NK与NPK处理的旱地红壤为研究材料,采用水分散-吸管法逐级提取了土壤中0.25～0.05 mm、0.05～0.01 mm、0.01～0.005 mm与<0.005 mm粒级微团聚体,对比分析了各粒级微团聚体中全磷、有效磷及磷活化系数的变化差异。基于土壤磷素分级结果,分析了旱地红壤中大小粒级微团聚体中极有效磷、中等有效磷及非有效磷对长期施肥的响应。借助于结构方程模型探讨了微团聚体中各形态磷、磷活化系数、颗粒组成、有机质、铁铝氧化物等因子与有效磷的互应关系。【结果】长期配施猪粪可以显著增加旱地红壤各粒级微团聚体中全磷、有效磷、磷活化系数及极有效磷、中等有效磷及非有效磷的含量,且<0.005 mm粒级微团聚体中的各组分磷增...     

不同利用方式旱地红壤水稳性团聚体及其碳、氮、磷分布特征

针对江西红壤地区不同利用方式引起的土壤质量和肥力的相应变化,研究了不同肥力水平、不同利用方式下红壤旱地水稳性团聚体含量及其养分分布规律。研究表明,荒地土壤中>5 mm水稳性团聚体含量显著高于其他利用方式,花生地和果园土壤则以0.25～0.053 mm的水稳性团聚体为主。各肥力水平下,菜地土壤中除>5 mm水稳性团聚体外,各粒级团聚体中有机碳、全氮和全磷含量均显著高于花生地、果园和荒地土壤。说明菜地土壤长期大量施肥,导致土壤碳、氮、磷养分含量均相对丰富。不同利用方式旱地红壤中,有机碳、全氮主要分布在>5 mm、5～2 mm和2～1 mm的较大粒径水稳性团聚体中。说明随着团聚体粒径增大,其有机碳含量增加,土壤全氮的消长趋势和有机碳一致。土壤全磷较均匀地分布在水稳性团聚体中,如高肥力菜地和荒地土壤各粒级团聚体中全磷含量间均无显著性差异。各利用方式旱地红壤中2～1 mm和1～0.5 mm的水稳性团聚体含量与土壤有机碳、全氮和全磷含量间均达到了极显著正相关。     

侵蚀程度对不同粒径团聚体中养分含量和红壤有机质稳定性的影响

以三种侵蚀程度的红壤(轻度、中度、严重)为供试材料,研究其>0.25 mm水稳性团聚体中的养分(全氮、全磷、全钾、有机质)和不同形态有机碳(易氧化态和难氧化态)的含量状况和分布特点。结果表明:随着红壤由轻度、中度到严重侵蚀的变化,>0.25 mm水稳性团聚体中有机质、全氮和全磷含量逐渐降低;>0.25 mm水稳性团聚体中的养分对土壤养分的贡献率为[>4 mm]>[0.5～1 mm]>[1～2 mm]>[2～4 mm]>[0.25～0.5 mm];侵蚀红壤的全氮、全磷和有机质均与>0.25 mm水稳性团聚体含量显著正相关。土壤有机碳、易氧化碳和难氧化碳的含量随着红壤侵蚀程度的增强逐渐降低,并且均与>0.25 mm水稳性团聚体、有机质、全氮、全磷呈极显著正相关。有机质氧化稳定性系数与不同粒径水稳性团聚体、土壤有机质、全氮和全磷均呈负相关。     

植被恢复对亚热带侵蚀红壤团聚体养分分布的影响

为深入了解不同植被恢复年限下土壤团聚体养分分布特征,以典型红壤侵蚀区福建省长汀县河田地区恢复年限分别为0,5,10,15,30,80a的坡地土壤为研究对象,分别对0—20cm和20—40cm土层不同粒径团聚体养分含量进行测定,并分析了它们与不同团聚体的相关关系。结果表明:(1)植被恢复过程中,土壤团聚体有机碳、全氮、全磷、全钾、速效磷和速效钾含量的变化范围分别为2.06~27.71g/kg,0.54~2.12g/kg,0.034~0.171g/kg,2.20~6.89g/kg,0.31~3.30mg/kg和7.35~85.71g/kg;(2)有机碳、全氮、全磷和速效磷含量随着团聚体粒径的减小总体上表现出显著升高趋势(P0.05),全钾和速效钾含量无明显差异(P0.05);(3)随植被恢复年限增加,各粒径团聚体中有机碳、全氮、全磷、速效磷含量总体上呈显著升高趋势(P0.05),全钾含量先升高后降低,而速效钾含量表现出波动增加趋势;(4)恢复初期(0a和5a)不同土层间团聚体养分含量无明显变化(P0.05),其它恢复年限0—20cm土层团聚体有机碳、全氮、全磷、速效磷和速效钾含量显著高于20—40cm土层(P0.05);(5)团聚体对土壤养分的贡献率表现为(5mm)(2~5mm)(0.5~1mm)(1~2mm)(0.25~0.5mm)(0.25mm),2mm粒径养分贡献率达34.18%~49.93%,土壤养分含量与0.25mm粒径相关性较强(P0.01)。植被恢复在降低土壤侵蚀的同时,土壤团聚体养分含量明显增加,土壤结构得以改善,养分固持能力得到加强。     

几种侵蚀红壤中有机质和团聚体的关系

以3种侵蚀红壤（轻度、中度、严重）为供试材料,利用干湿筛法获得其不同粒径的水稳性团聚体（〉4 mm,2～4 mm,1～2 mm,0.5～1 mm,0.25～0.5 mm）,分析团聚体中有机质的分布。结果显示,随着侵蚀程度的增强,〉0.25mm水稳性团聚体的含量逐渐下降,而团聚体的分散度却依次升高;无论何种侵蚀程度,湿筛后团聚体的组成均以小粒径团聚体（〈0.25 mm）占优势。轻度和中度侵蚀的红壤,其有机质含量随着团聚体粒径的增大而增大,严重侵蚀的则相反;侵蚀红壤有机质含量和〉0.25 mm水稳定性团聚体呈显著正相关,3种侵蚀红壤团聚体对土壤有机质的贡献为轻度侵蚀〉中度侵蚀〉严重侵蚀。     

长期施肥对旱地红壤团聚体磷素固持与释放能力的影响

在中国科学院红壤生态试验站26年的旱地红壤长期肥料定位试验中,选取无机肥试验区的NPK、NK处理,有机无机肥配施试验区的对照(CK)、CK+稻秆(RS)、CK+花生秸秆还田(PS)、CK+绿肥(FR)及CK+猪厩肥(PM)等7个肥料处理土壤,采用湿筛法逐级提取并得到粒级依次为2 mm、2~1 mm、1~0.25 mm与0.25~0.053 mm的团聚体土壤样品;通过室内分析获得了土壤及各粒级团聚体的全磷(TP)、有效磷(Available P)、水溶性磷(CaCl_2-P)、土壤磷素吸持指数(PSI)及土壤磷素饱和度(DPS)等指标值,并探讨了上述测定指标间的相关关系。结果表明:长期施用磷肥可有效保持旱地红壤的供磷水平,配施猪厩肥可显著增加旱地红壤及大小团聚体的TP、有效磷及CaCl_2-P含量、降低土壤PSI并显著增大旱地红壤DPS,加大了旱地红壤磷素的流失风险;随着土壤中1 mm粒级团聚体数量的增多,旱地红壤磷素储量显著增加,磷素固持能力显著下降,土壤磷释放潜能随之增大。由DPS、有效磷及CaCl_2-P的分段线性拟合方程可以推断得出,当旱地红壤中有效磷为168~260 mg kg~(-1)时或DPS28%,土壤磷素具有潜在流失风险;当有效磷≥260 mg kg~(-1)或DPS≥28%,土壤磷素具有极高的流失风险,应立即停止施用磷肥尤其是有机磷肥,并重新调整施肥方案,以避免土壤磷素流失及其对水体环境的污染。     

有机肥增减施后红壤水稻土团聚体有机碳的变化特征

利用一个长达35 a水稻土长期定位试验,在保证原有定位试验继续正常开展的前提下,将部分原化肥处理增施有机肥,部分原有机肥处理改施化肥或者增施有机肥,研究有机肥增减施后长期不同施肥红壤性水稻土团聚体有机碳变化特征及影响。结果显示:红壤性水稻土以0.25 mm团聚体为主;长期不同施肥下土壤团聚体有机碳含量高低排序均表现为:0.25 mm团聚体0.25～0.053 mm团聚体(0.053 mm)团聚体,长期施用有机肥可提高红壤水稻土各粒级有机碳含量和2mm团聚体有机碳的贡献率。施肥对红壤性水稻土各粒径团聚体有机碳影响的大小排序为:0.053 mm0.25～0.053 mm(2 mm)2～0.25 mm;游离氧化铁和络合态铝对2～0.25 mm粒径团聚体有机碳有着重要影响,游离氧化铁在2 mm团聚体的形成中发挥作用。增加有机肥施用量可提高2 mm各粒级团聚体有机碳含量,减施有机肥则显著降低各粒级团聚体有机碳含量。不管减施还是增施有机肥均导致2mm团聚体有机碳贡献率降低;同时,减施有机肥后2 mm的各级团聚体有机碳贡献率提高,而增施有机肥后2～0.25 mm团聚体有机碳贡献率提高。     

长期施肥对红壤性水稻土团聚体特征的影响

利用干筛法研究了红壤性水稻土连续施肥23年后,各粒径土壤团聚体含量及其特征值变化.结果表明:施用有机肥(NPKM、OM)与CK相比,可提高粒径3～5 mm土壤团聚体含量25.4%～24.6%,并分别降低粒径0.05～0.25 mm、＜0.05mm团聚体含量70.6%～71.1%和113.6%～121.7%,差异达显著水平;单施化肥处理(N、NP、NPK)与CK相比,各粒径土壤团聚体含量无明显差异,表明施肥对红壤性水稻土团聚体的影响主要受有机肥的控制.施用有机肥显著提高红壤性水稻土团聚体的平均重量直径(MWD)、几何平均直径(GMD),而降低团聚体的分形维D值:水稻土团聚体的MWD、GMD与＞3 mm团聚体含量和水稻年产量存在显著的正相关,而团聚体分形维D值则与＞3 mm团聚体含量和水稻年产量存在显著的负相关,因而土壤团聚体的MWD、GMD和分形维D值可较客观地反映土壤肥力的状态.不同施肥处理的水稻年产量与粒径3～5 mm团聚体含量呈显著正相关而与0.05～0.25 mm、＜0.05 mm团聚体含量呈显著负相关,表明红壤性水稻土团聚体干筛分析条件下,粒径3～5 mm、0.05～0.25 mm、＜0.05 mm团聚体含量可作为红壤性水稻土土壤肥力表征的物理性指标.     

不同施肥处理对红壤水稻土团聚体有机碳分布的影响

通过23年的长期田间定位试验观测,研究了施肥对红壤水稻土不同发生层团聚体组成、有机碳含量及有机碳库分布的影响.试验设不施肥（CK）、无机肥（NPK）、有机肥（猪粪＋紫云英）（OM）和无机肥与有机肥配施（NPKM）等4个处理.结果表明,＞3 mm团聚体含量随土层深度呈增加趋势,而其他粒径的团聚体含量则呈下降趋势.施肥处理有利于1～3 mm和0.25～1 mm团聚体含量增加.各处理中,各层同粒径团聚体有机碳含量从高到低的顺序为：A＞P＞W1、W2.不同粒径团聚体中有机碳含量有明显差异,除＜0.05 mm团聚体外,粒径愈细,有机碳含量愈高.1～3 mm和0.25～1 mm团聚体含量与全土有机碳含量呈显著正相关.不同施肥处理有机碳储量表现出NPKM＞OM＞NPK＞CK的趋势.同施肥处理同发生层不同粒径团聚体有机碳储量从高到低的顺序为：＞3 mm、1～3 mm、0.25～1 mm、0.05～0.25 mm和＜0.05 mm,且差异显著.施肥处理增加的新碳主要向1～3 mm、0.25～1 mm团聚体富集.     

侵蚀红壤腐殖酸组分特点及其对水稳性团聚体的影响

在华中地区采集不同侵蚀程度(轻度、中度、严重)的红壤,利用干湿筛法获得不同粒径的水稳性团聚体(0.25 mm)。通过测定不同粒径水稳性团聚体(0.25 mm)的腐殖酸组分(胡敏酸和富里酸)碳量,以及向不同侵蚀程度的各粒径团聚体(0.25 mm)中添加不同浓度(0.06、0.6 g L-1)的胡敏酸,分析侵蚀红壤腐殖酸组分特点及其对水稳性团聚体的影响。结果表明:随着侵蚀程度的增加,供试红壤和水稳性团聚体(0.25 mm)的腐殖酸组分含量减少;轻度和中度侵蚀红壤中,大粒径(4和2~4 mm)水稳性团聚体的胡敏酸碳量较小粒径(1~2、0.5~1和0.25~0.5 mm)的高;同种侵蚀程度下,除严重侵蚀红壤中2~4 mm团聚体的富里酸碳量与1~2 mm团聚体中的有显著差异外,不同粒径团聚体中富里酸碳量差别不大。3种侵蚀程度红壤的胡富比总体上小于1,其中中度侵蚀红壤和各粒径水稳性团聚体的胡富比均大于其他两种侵蚀程度的。腐殖酸组分与水稳性团聚体(0.25 mm)含量呈极显著正相关,与胡敏酸碳量的相关系数最大(r=0.85**,n=19)。添加不同浓度(0.06和0.6 g L-1)胡敏酸后,3种侵蚀程度红壤各粒径水稳性团聚体(0.25 mm)的含量均增加,且小粒径团聚体(1~2、0.5~1和0.25~0.5 mm)的增加量要高于大团聚体(2~4、4 mm)的。     

Soil-profile distribution of primary and secondary plant-available nutrients under conventional and no tillage

Nutrient distributions under no tillage (NT) compared with conventional disk-and-bed tillage (CT) management in the warm, humid region of the southeastern USA need to be assessed so that future placement, quantity, and type of fertilizers can be altered, if necessary, to efficiently match crop demands. We determined soil-profile distributions of pH, N, P, S, K, Ca, Mg, Na, Zn, Fe, Mn, and Cu to a depth of 0.9 m at the end of 8.5 years of continuous CT and NT management on a Weswood silty clay loam (fine, mixed, thermic Fluventic Ustochrept) in southcentral Texas. Most dramatic changes occurred within the 0–0.05 m depth, where soil under NT had lower pH, Fe, and Cu than under CT, but greater P, K, Zn, and Mn. Greater P and K under NT than under CT also occurred below the till-zone (0.15–0.3 m). At a depth of 0–0.3 m, soil under NT contained greater amounts of extractable P, K, Zn, Fe, Mn, and Cu than under CT. Nitrogen fertilization had little effect on nutrient distributions, except resulting in greater extractable K at 0–0.05 m and greater nitrate at 0–0.15 m. Few changes in soil-profile distributions were observed for extractable S, Ca, Mg, and Na. Long-term continuous use of NT on this fine-textured, high-fertility (except for N) soil had no apparent adverse effects on nutrient distributions relative to CT, but enhanced conservation and availability of P, K, Zn, Fe, Mn, and Cu near the soil surface where crop roots proliferate.     

Assessing the effectiveness of conservation measures: Resolving the “wicked” problem of the Steller sea lion

“Wicked” problems are those that are complex and that change when solutions are applied. Many conflicts in conservation fall into this category. The study approached the problem of how to constrain the apparent wickedness of a problem in the conservation management of a species by using simple empirical indicators to carry out iterative assessment of the risk to a population and to document how this risk evolves in relation to the addition of new data and the implementation of management actions. Effects of high levels of uncertainty within data and also concerning population structure were examined through stochastic simulation and by exploration of scenarios. Historical trends in the example used, the Steller sea lion, showed rapid declines in abundance in some regions during the 1980s. The current total population is 130,000-150,000 Steller sea lions through Alaska and British Columbia and this number has been stable since about 1990 in spite of regional differences in population dynamics. Regional differences in the sequence of changes in the number of pups and non-pups, suggested that an internal re-distribution of juveniles could have happened between 1980 and 1990. Current productivity also appears close to the long-term mean. Stochastic population projection using various scenarios showed that, based upon this history, the risk of extinction for the population has declined and is below reasonable thresholds for considering the population to be endangered. The trends in risk suggest that management actions taken since 1990 have probably been effective. Consequently, the conservation management objectives for the Steller sea lion are probably being met. The approach provides a mechanism, based upon experience and scenario analysis, for exploring future policy options and may help to constrain the debate amongst stakeholders about the cost-benefit trade-offs associated with different options.     

“绿色革命”以来大气CO2浓度升高对C3作物营养品质的影响

自20世纪60年代"绿色革命"以来,育种技术和农耕技术的发展促进了农作物产量的大幅提升,然而作物的营养品质出现下降趋势。在相似的遗传背景下,大气CO_2浓度升高会使单位体积农作物产品的营养元素含量下降,因此"绿色革命"至今,农作物产品的营养元素下降可能受大气CO_2浓度升高影响。通过植物生长箱模拟"绿色革命"初期和目前的大气CO_2浓度水平(310μmol/mol和400μmol/mol),针对主要C_3作物水稻、小麦和大豆,研究"绿色革命"以来大气CO_2浓度升高对其籽粒的C、N、Fe、Zn元素含量的影响,结果表明:CO_2浓度升高对3种作物籽粒的C元素含量几乎没有影响,变化幅度在±1.5%之间;籽粒的N、Fe、Zn元素含量普遍呈现下降趋势,但均未达到显著水平。     

Potential of wild species for genetic enhancement of some semi-arid food crops

Discovery and incorporation of genes from wild species provide means to sustain crop improvement, particularly when levels of resistance in the cultigens are low and virulent strains of pests and pathogens overcome the host plant resistance. The extent of utilization and the potential of the wild genepool for genetic enhancement were reviewed in five important food crops viz. sorghum, pearl millet, chickpea, pigeonpea and groundnut grown in the semi-arid tropics. Introgression from compatible wild germplasm in the primary gene pool resulted in transfer of new cytoplasmic male sterility systems in pearl millet and pigeonpea, development of high protein, cleistogamous flower and dwarf pigeonpea lines and foliar disease resistant groundnut cultivars. Utilization of wild species in secondary and tertiary gene pools has been generally limited due to sterility, restricted recombination or cross incompatibility. Nevertheless, these species are extremely important as they contain high levels of resistance to several important biotic and abiotic stresses. Several of them, like those belonging to the Parasorghum section and the rhizomatous Arachis species are sources of multiple resistances and hold great promise to sustain crop productivity.     

Enhanced establishment and growth of giant cardon cactus in an eroded field in the Sonoran Desert using native legume trees as nurse plants aided by plant growth-promoting microorganisms and compost

To evaluate the feasibility of long-term desert reforestation technology of mixed vegetation, cardon cactus (Pachycereus pringlei) seedlings from indoor and outdoor nurseries were planted in the field adjacent to one seedling of potential legume nurse trees: mesquite amargo (Prosopis articulata), yellow palo verde (Parkinsonia microphylla), and blue palo verde (Parkinsonia florida). Some of the planting holes were also supplemented with common dairy compost. Additionally, the combinations of legume tree–cactus were inoculated with either a consortium of desert arbuscular mycorrhizal (AM) fungi, plant growth promoting bacteria (PGPB; the diazotroph Azospirillum brasilense Cd, and the phosphate solubilizer Paenibacillus sp.), or a mixture of all. The field experiments were evaluated periodically during 30 months for survival and growth. Cardons reared in an outdoor screen house survived better in the field than those reared in a controlled growth chamber and hardened later outdoors. Association with any legume nurse tree increased survival and enhanced growth of untreated cardons. For cardons growing alone, application of either compost, AM fungi, and all the treatments combined increased survival. For these plants, no treatment affected plant growth during the first 3 months after transplanting. Later, all treatments, except for AM fungi, enhanced plant growth. However, only 2 years after transplanting the enhanced growth effect of AM fungi was also significant. In the presence of the legume nurse trees, transient positive effects on cardon growth were recorded. General evaluation after 30 months of cultivation showed that the treatments positively affected cardon growth when growing alone or in combination only with mesquite amargo but not with the other two legume trees. This study proposes that young legume trees have the capacity to enhance survival and growth of cardon cactus, depending on the legume cactus combination. Additional treatments such as compost or PGPB can either amplify the effect or else attenuate it.     

Karyotypic and genome size variation in Cajanus cajan (L.) Millsp. (pigeonpea) and some wild relatives

Detailed karyotypes and 4C DNA amounts have been studied in five cultivars of Cajanus cajan and 20 species belonging to Cajanus, Rhynchosia, Dunbaria, Flemingia and Paracalyx. C. cajan shows intraspecific variability and its karyotype is most similar to that of C. cajanifolius (sect. Cajanus) in the morphology and number of satellite chromosomes and the lack of any correlation between chromosome size within the complement and asymmetry. Karyotypes of C. lineatus and C. sericeus belonging to sect. Atylia are similar with respect to maximum r-index and the ratio of longest and shortest chromosomes in their respective complements. C. acutifolius (sect. Frutocosa) is distinct in having a very low ratio (1.44) between the longest and the shortest chromosomes, while in C. albicans, C. goensis, C. scarabaeoides (sect. Cantharospermum) there is no chromosome pair with r-index >2.0. C. mollis and C. volubilis (sect. Volubilis) show similarity with regard to ratio between the longest and the shortest chromosomes and a distinct chromosome pair with an arm ratio of 2.7 and C. platycarpus is distinct (sect. Rhynchosoides) in having the smallest ratio (1.36) between the longest and the shortest chromosomes. 4C DNA amounts in Cajanus and Rhynchosia vary between 3.28 pg to 11.69 pg and 4.92 pg to 11.13 pg respectively, while in Dunbaria, Flemingia and Paracalyx these vary between 4.70 and 7:06 pg. In the genus Cajanus karyotypic features and 4C DNA amounts agree with the sectional classification and bring out a close relationship between C. cajan and C. cajanifolius. This is supported by studies based on seed protein patterns, isozyme analysis, trypsin and chymotrypsin inhibitor patterns, RFLP and RAPD data and crossability relationships. A clear relation between DNA amount and abnormalities effecting the extent of pairing and recombination in interspecific hybrids shows the importance of this study in developing future breeding programme involving C. cajan and its wild relatives that are of potential value.     

Faculty positions:Center for Agricultural Resources Research,Chinese Academy of Sciences

正The Center for Agricultural Resources Research(CARR),the Institute of Genetics and Developmental Biology(IGDB),Chinese Academy of Sciences,invites applicants for several research group leader positions.CARR is one of the research organizations in Chinese Academy of Sciences(CAS).We seek nominations and applications     

Effect of some fungicide seed treatments on the survival of Bradyrhizobium japonicum and on the nodulation and yield of soybean [Glycine max. (L) Merr.]

Several commercial fungicide seed treatments were evaluated for their possible effect on the survival of Bradyrhizobium japonicum on seeds and on the nodulation and yield of soybeans in a greenhouse and a field experiment. quinolate Pro (carbendazim and oxine copper), Vitavax 200FF (carboxin and thiram), and Monceren (pencycuron) had a small effect or no effect on the survival of B. japonicum and on the nodulation and yield of soybeans. They can thus be considered compatible with soybean seed inoculation. Germipro UFB (carbendazim and iprodione), Apron 35J (metalaxyl), and Tachigaren (hymexazol) decreased B. japonicum survival and the nodulation and yield of soybeans and thus cannot be considered compatible with soybean seed inoculation.     

Einfluss der düngungsmethoden auf die entwicklung von zuckerrübenkrankheiten

Während neunjähriger (1994-2002) Untersuchungen in der Versuchsanstalt Ba?cyny (Fahlerde, Fall-Gleyboden, ausgebildet aus leichtem Lehmboden) wurde der Befall mit Krankheiten an der Zuckerrübe in Abhängigkeit von der Düngungsmethode bewertet. Folgende Methoden wurden angewendet: A - Mineraldünger, B - Stalldung und Mineraldünger, C - Stroh-, Gründung und Mineraldünger, D - Kompostdünger und Biopräparate (ökologische Düngung). Auf den Objekten A, B, C wurden bei konstanter Phosphor-Kalium-Düngung die Stickstoffgaben von 0 bis 180 kg.ha^?1 differenziert. Auf dem ökologischen Objekt D ohne Mineraldünger wurden statt Stickstoff die Biopräparate P₅₀₀ und P₅₀₁ verabreicht. Der geringste Befall von Krankheiten wurde an der Zuckerrübe auf jenem Objekt festgestellt, das mit Kompost und den Biopräparaten gedüngt worden war. Im Rahmen der konventionellen Düngungsmethoden wurde allgemein die höchste Intensität der Krankheiten an der mit Stroh, Gründung und Mineraldünger gedüngten Zuckerrübe beobachtet. Ausnahmen von dieser Regel bildete das Vorkommen von echtem Mehltau und Keimlingsfäule. Auf den Objekten A, B, C nahm die Intensität der Krankheiten mit der Steigerung der Stickstoffgabe zu. Auch die auf Objekt D verabreichten Biopräparate stimulierten die Entwicklung der Krankheiten. The effect of various fertilization methods on the diseases of sugar beet was estimated on the basis of nine-year studies (1994-2002) conducted in the Experimental Production Farm in Ba?cyny on loess-based, sedimentary-gley, light-clay soil. The following fertilization methods were applied: A - mineral fertilization, B - mineral and manure fertilization, C - straw, green manure and mineral fertilization, D - ecological compost and biodynamic preparations fertilization. In the objects A, B and C fertilized with phosphorus and potassium, various doses of nitrogen ranging from 0 to 180 kg/ha^?1 were used. In the ecological object D, where mineral fertilization was not used, biodynamic preparations P₅₀₀ and P₅₀₁ were applied instead of nitrogen. The lowest ratio of the diseases of sugar beet was observed in the object fertilized with the compost and biodynamic preparations. In view of the conventional fertilization methods, generally the highest incidence of diseases was noted in the sugar beet fertilized with straw, green manure and mineral fertilizers. The exception was the occurrence of powdery mildew and the black leg. In objects A, B and C the incidence of the diseases increased with the increase in nitrogen doses. The applied biodynamical preparations in the object D stimulated the development of diseases too.     

Prospects for control of Pythium damping-off of lettuce with Trichoderma,Gliocladium and Enterobacter spp.

Summary Damping-off of lettuce (Lactuca sativa) caused by Pythium ultimum was studied in pots containing a non-sterile potting mix in the glasshouse. Fifty P. ultimum sporangia g^-1 compost reduced the plant stand to 15% and shoot dry weight to 18%, but this reduction was totally prevented by applying Trichoderma harzianum at 2×10⁵ viable propagules g^-1 potting mix. Gliocladium virens and Enterobacter cloacae also alleviated the damping-off. E. cloacae did not adversely affect the action of the fungal antagonists. The co-existence of the bacterial and fungal antagonists was revealed on the root surface and inner surface of the testa by scanning electron microscopy, indicating the compatibility of the biocontrol agents.