侵蚀红壤中结合态腐殖质及其与水稳性团聚体的关系

以亚热带地区3种侵蚀程度(依据其植被、坡度以及土层厚度不同分为轻度、中度和严重侵蚀)的红壤为供试材料,利用湿筛法获得粒径4、2~4、1~2、0.5~1、0.25~0.5 mm的水稳性团聚体,分析侵蚀红壤和团聚体中3种结合态(紧结态、松结态和稳结态)腐殖质含碳量的分布特征,及其对0.25 mm团聚体稳定性的影响。结果表明:随着红壤侵蚀程度的增强,3种结合态腐殖质的含碳量均逐渐减小。在轻度侵蚀的红壤中,随着团聚体粒径的增大,其紧结态和松结态腐殖质的含碳量逐渐升高,稳结态腐殖质的含碳量逐渐降低;但是在中度和严重侵蚀红壤中,不同粒径团聚体的3种结合态腐殖质的含碳量基本相同。侵蚀红壤的紧结态、松结态腐殖质的含碳量与其0.25 mm水稳性团聚体的含量均呈极显著正相关(r分别为0.88,0.74),说明土壤中腐殖质与矿物质的结合方式和松紧程度不一样,其对侵蚀红壤的结构具有不同的影响。     

几种侵蚀红壤中有机质和团聚体的关系

以3种侵蚀红壤（轻度、中度、严重）为供试材料,利用干湿筛法获得其不同粒径的水稳性团聚体（〉4 mm,2～4 mm,1～2 mm,0.5～1 mm,0.25～0.5 mm）,分析团聚体中有机质的分布。结果显示,随着侵蚀程度的增强,〉0.25mm水稳性团聚体的含量逐渐下降,而团聚体的分散度却依次升高;无论何种侵蚀程度,湿筛后团聚体的组成均以小粒径团聚体（〈0.25 mm）占优势。轻度和中度侵蚀的红壤,其有机质含量随着团聚体粒径的增大而增大,严重侵蚀的则相反;侵蚀红壤有机质含量和〉0.25 mm水稳定性团聚体呈显著正相关,3种侵蚀红壤团聚体对土壤有机质的贡献为轻度侵蚀〉中度侵蚀〉严重侵蚀。     

侵蚀程度对不同粒径团聚体中养分含量和红壤有机质稳定性的影响

以三种侵蚀程度的红壤(轻度、中度、严重)为供试材料,研究其>0.25 mm水稳性团聚体中的养分(全氮、全磷、全钾、有机质)和不同形态有机碳(易氧化态和难氧化态)的含量状况和分布特点。结果表明:随着红壤由轻度、中度到严重侵蚀的变化,>0.25 mm水稳性团聚体中有机质、全氮和全磷含量逐渐降低;>0.25 mm水稳性团聚体中的养分对土壤养分的贡献率为[>4 mm]>[0.5～1 mm]>[1～2 mm]>[2～4 mm]>[0.25～0.5 mm];侵蚀红壤的全氮、全磷和有机质均与>0.25 mm水稳性团聚体含量显著正相关。土壤有机碳、易氧化碳和难氧化碳的含量随着红壤侵蚀程度的增强逐渐降低,并且均与>0.25 mm水稳性团聚体、有机质、全氮、全磷呈极显著正相关。有机质氧化稳定性系数与不同粒径水稳性团聚体、土壤有机质、全氮和全磷均呈负相关。     

不同修复措施下红壤水稳性团聚体中有机质分布特征

试验设置作物对照(CK)、作物+PAM(聚丙烯乙酰)(C1)、作物+稻草覆盖(C2)、作物+带状牧草(C3)、作物+PAM+带状牧草(C4)和作物+稻草覆盖+带状牧草(C5)6个处理,分别于种植花生前(A)、花生收获后(B)和小麦收获后(C)采集表层土样,研究不同修复措施下鄂东南侵蚀红壤团聚体的稳定性及水稳性团聚体中有机质的分布特征。结果表明,C时期各处理>0.25mm水稳性团聚体含量的平均值比A时期提高了24.78%,以C2处理的水稳性团聚体含量最高。不同时期土壤和各级水稳性团聚体中有机质含量表现为B>C>A。随着团聚体粒径(>4mm,4～2mm,2～1mm,1～0.5mm,0.5～0.25mm)减小,水稳性团聚体中有机质含量逐渐降低。土壤有机质含量与各粒径水稳性团聚体有机质含量呈线性正相关,小粒径(2～0.25mm)水稳性团聚体的有机质含量与土壤有机质含量呈极显著正相关(相关系数为0.779 2～0.828 6)。综合考虑土壤有机质含量和团聚体稳定性2个因素,稻草覆盖措施对侵蚀红壤的修复效果较好。     

苜蓿作物轮作模式对土壤团聚体稳定性及有机碳的影响

本研究以苜蓿?作物轮作试验为研究对象,探讨了苜蓿?苜蓿(L-L)、苜蓿?休闲(L-F)、苜蓿?小麦(L-W)、苜蓿?玉米(L-C)、苜蓿?马铃薯(L-P)和苜蓿?谷子(L-M)6种轮作模式对陇中黄土高原雨养农田苜蓿土壤团聚体稳定性以及土壤总有机碳含量的影响。结果表明:不同轮作模式下土壤机械稳定性团聚体以≥0.25 mm团聚体为优势团聚体,均占72.17%以上,而土壤水稳性团聚体以0.25 mm团聚体为优势团聚体,均占95.18%以上。随着土层深度的增加,各处理≥0.25 mm的团聚体数量及平均重量直径(MWD)均随之增加,而水稳性大团聚体数量及MWD值无明显规律性。与L-L处理相比,L-C和L-P处理0~30 cm耕层土壤≥0.25 mm的团聚体含量分别增加5.94%和1.12%,L-C处理的MWD表现为最高,而其他轮作处理则不同程度降低了≥0.25 mm团聚体含量及MWD;随着土层深度的增加,6种不同轮作模式的土壤有机碳含量均呈现逐渐降低的趋势,在0~30 cm的耕层土壤,较之L-L处理,L-W、L-C、L-P和L-M处理均从不同程度上降低了土壤有机碳含量,其中L-P处理有机碳含量最低,降低了18.68%。相关性分析表明,土壤总有机碳分别与2~5 mm、1~2 mm、0.5~1 mm和0.25~0.5 mm粒径的水稳性团聚体比例以及MWD表现出极显著正相关,而与0.25 mm粒径的水稳性团聚体呈极显著负相关。综上所述,苜蓿?玉米轮作模式能明显增加土壤团聚体机械稳定性,而不同苜蓿?作物轮作模式对土壤团聚体的水稳性影响较小,土壤有机碳含量在很大程度上影响着土壤水稳性团粒结构的形成与稳定性,二者密切相关。     

长期施肥下不同种植年限碱化草甸土水稳性团聚体及有机碳分布

以松嫩平原碱化草甸土为研究对象,通过长期施用有机肥,对不同种植年限碱化草甸土水稳性团聚体及有机碳的粒径分布特征进行了研究。结果表明,随着种植年限的增加,土壤较大粒级水稳性团聚体(>1mm)含量减少,而0.25~0.5mm粒级水稳性团聚体含量明显增加随种植年限的增加,各粒级碱化草甸土有机碳平均含量呈增加趋势。2~5mm粒级土壤有机碳平均含量最高,随粒级的减小,有机碳含量逐渐减少,0.25~0.5mm粒级水稳性团聚体含量与有机碳含量呈现显著正相关关系种植4a后,碱化草甸土土壤水稳性团聚体及有机碳含量开始呈现稳中有升的趋势。     

化肥与有机物料配施对黄褐土团聚体分布及有机碳含量的影响

通过对黄褐土小麦/玉米轮作定位试验,研究了不同施肥模式对土壤团聚体分布、稳定性及团聚体中有机碳含量、各级团聚体对有机碳的贡献率的影响。结果表明:黄褐土的水稳性团聚体组成主要以0.25 mm粒径为主,施有机物料增加了0.25 mm水稳性团聚体,同时提高团聚体的稳定性。团聚体有机碳主要存在于0.25 mm水稳性团聚体中,并随着水稳性团聚体粒径的增大而明显增加。化肥配施秸秆对黄褐土水稳性团聚体影响较大,显著增加0.053 mm各粒径水稳性团聚体含量,降低0.053 mm水稳性团聚体含量,同时显著提高团聚体平均重量直径(MWD)、几何平均直径(GMD)和稳定率(R0.25),并且化肥配施秸秆对土壤中0.25 mm各粒径团聚体对有机碳的贡献率增加更为明显。而化肥配施鸡粪对土壤中0.25 mm各粒径团聚体中有机碳增加效果最好。     

施用生物质炭对黑土腐殖质组成及水稳性团聚体分布的影响

为探究生物炭对土壤腐殖质组成和团聚体特征的影响，以东北黑土区植烟土壤为研究对象，设置了3个处理，2019-2020年连续施用低量生物炭5t/hm2（C1）；高量生物炭25t/hm2（C2）和不施生物炭（CK），分析了不同用量生物炭对土壤腐殖质组分及水稳性团聚体分布的影响，并利用傅里叶红外光谱(FTIR)和13C核磁共振光谱(13C-NMR)对土壤胡敏酸化学结构进行表征。结果表明：C1和C2处理分别使富里酸减少了16.90%和40.85%，胡敏酸含量显著增加了14.86%和33.78%，胡敏酸在腐殖酸中所占比例（PQ值）也显著增加；FTIR和13C-NMR分析表明，C2处理的土壤胡敏酸的2920/1620值降低了11.82%，脂族C/芳香C比值降低了13.04%，表明高量生物炭使胡敏酸芳构化程度增强，脂肪结构比例降低；生物炭的添加促使土壤大团聚体（>0.25mm）比例增加，C2处理提升大团聚体的作用更显著。结合相关性分析发现，胡敏酸含量与2~0.25mm大团聚体含量显著正相关，胡敏酸分子的脂肪族官能团特征与>2mm粒级团聚体显著正相关。此外，C1和C2处理显著提高了烟叶产量。从而表明，生物炭能提升土壤腐殖质中胡敏酸含量和结构，有利于土壤大团聚体形成，提高土壤固碳潜力，对作物有一定的增产效果。     

不同利用方式旱地红壤水稳性团聚体及其碳、氮、磷分布特征

针对江西红壤地区不同利用方式引起的土壤质量和肥力的相应变化,研究了不同肥力水平、不同利用方式下红壤旱地水稳性团聚体含量及其养分分布规律。研究表明,荒地土壤中>5 mm水稳性团聚体含量显著高于其他利用方式,花生地和果园土壤则以0.25～0.053 mm的水稳性团聚体为主。各肥力水平下,菜地土壤中除>5 mm水稳性团聚体外,各粒级团聚体中有机碳、全氮和全磷含量均显著高于花生地、果园和荒地土壤。说明菜地土壤长期大量施肥,导致土壤碳、氮、磷养分含量均相对丰富。不同利用方式旱地红壤中,有机碳、全氮主要分布在>5 mm、5～2 mm和2～1 mm的较大粒径水稳性团聚体中。说明随着团聚体粒径增大,其有机碳含量增加,土壤全氮的消长趋势和有机碳一致。土壤全磷较均匀地分布在水稳性团聚体中,如高肥力菜地和荒地土壤各粒级团聚体中全磷含量间均无显著性差异。各利用方式旱地红壤中2～1 mm和1～0.5 mm的水稳性团聚体含量与土壤有机碳、全氮和全磷含量间均达到了极显著正相关。     

秸秆还田对土壤微团聚体特征的影响

采用尼龙网袋法进行秸秆还田的原位模拟,比较不同秸秆还田量在不同深度下还田对土壤微团聚体组成及稳定性、微团聚体有机碳及腐殖酸组分的影响。试验设置秸秆还田量R0(0)、R1(0.44%)、R2(0.88%)、R3(1.32%)和0—15(S1),15—30(S2),30—45(S3)cm 3个不同还田深度交叉处理。结果表明:0.25~0.02mm的微团聚体为优势粒级,秸秆还田使各土层0.25~0.02,0.25mm的团聚体含量增加,而0.02~0.002,0.002mm的微团聚体含量减少,且各粒级含量的变化幅度均在S1层最大。不同秸秆量处理下不同土层,不同粒级的微团聚体的平均重量直径(MWD)较无秸秆还田均有所增加。同时,施用不同量秸秆能提高土壤各粒级微团聚体中有机碳及腐殖酸含量,且在同一土层中随粒级减小,有机碳及腐殖酸含量增加,而在不同土层内,有机碳及腐殖酸含量表现为S1S2S3。胡敏酸与富里酸比值对于不同土层,不同粒级的微团聚体则呈现不同的变化趋势。秸秆还田能促使土壤微团聚体向更大粒级的团聚体转化,有效增强土壤肥力的同时改善了土壤结构。     

Endogenous organic acid variations in different chickpea (Cicer arietinum L.) genotypes

Abstract

Changes in cropping systems during the past century have led to selective pressure on weed flora. Species and ecotypes with characteristics enabling them to survive in high-input farmland have increased in numbers, at the cost of plants lacking these characters. Since the 1950s, the perennial weed species Cirsium arvense (L.) Scop. and Sonchus arvensis L. have mainly been controlled by the herbicide group synthetic auxins like MCPA. During recent decades, C. arvense seems to have become less susceptible to MCPA in both Europe and North America but the reasons are unclear.

To study the importance of selective pressure on weed ecotypes, both short- and long-term studies were carried out in Uppsala, Sweden. The first consisted of two growth-characteristic greenhouse experiments. The hypothesis was that ecotypes of C. arvense and S. arvensis from high-input farmland were different and displayed a more competitive growth pattern than did ecotypes from low-input farmland.

The second study was a field experiment with four ecotypes of C. arvense from low-input farmland to study if selective pressure was in force, over a period of six years. The four ecotypes had different growth characteristics and herbicide sensitivity and they were exposed to crop competition and MCPA treatments during the experimental period. The hypothesis was that ecotypes with a more competitive growth pattern and MCPA tolerance would survive to a greater extent than would other ecotypes. For C. arvense, the results from the growth-characteristic experiment showed that the growth pattern of ecotypes from high-input farmland differed, showing a more directly elongated growth pattern with fewer spines on the leaves compared with ecotypes from low-input farmland, which usually were of rosette-type. Results from the field experiment with C. arvense showed that after six years MCPA-sensitive and/or rosette-type ecotypes had almost disappeared while ecotypes with a more directly elongated growth pattern and less sensitive to MCPA survived to a much greater extent. The conclusion was therefore that when exposed to selective pressure like crop competition and herbicide treatments, ecotypes of C. arvense with a more directly elongated growth pattern and less sensitive to herbicide treatment survived to a greater extent compared with ecotypes missing these traits. Ecotypes from high-input farmland had generally fewer leaf spines than did ecotypes from low-input farmland. This may suggest a trade-off between spine formation and rapid competitive growth. In the growth-characteristic experiment with S. arvensis, no differences between ecotypes from high- and low-input farmland regarding growth characteristics or leaf spines could be detected. This might partly be due to a lower exposure of S. arvensis to selective pressure compared with C. arvense, since S. arvensis generally is less sensitive to MCPA.     

有机酸处理条件对玉米秸秆热解特性的影响

酸洗预处理能有效改善K^+对生物质热解的影响,该文利用热重分析仪和裂解-气相色谱质谱联用仪进行了玉米秸秆的热解试验,研究了不同有机酸酸洗浓度(3%、5%和7%)、酸洗温度(25、50和75℃)和酸洗时间(1、2和3 h)对玉米秸秆热解特性的影响。结果表明:酸洗能显著降低玉米秸秆内在K^+的含量;经过不同条件的有机酸洗预处理后,玉米秸秆的TG/DTG(thermogravimetry/differential thermogravimetry)曲线均向高温段移动,最大热解速率随着酸洗浓度和酸洗温度的增加逐渐增大,随着酸洗时间的增加先增大后降低,在酸洗温度为75℃时,最大热解速率达到最大值15.49%/min;与此同时,玉米秸秆热解主要产物为酚类、酮类和呋喃类化合物,酸洗后,其酚类物质产率明显增加,在酸洗浓度为7%时达到最大值16.75%,而酮类和呋喃类化合物产率减少,分别在酸洗时间为1 h和酸洗浓度为7%时达到最小值0.10%和7.13%。酸洗后,焦炭产率减少,在酸洗浓度为3%时达到最小值18.79%。通过研究不同处理条件下有机酸对玉米秸秆热解特性的影响,为生物质预处理中酸溶液的选择提供了参考。     

Organic Acids and Diffusive Flux of Organic and Inorganic Phosphorus in Sandy-Loam and Clayey Latosols

This study evaluated the effects of organic acids on the diffusive flux of phosphorus (DFP) along time. Treatments were a factorial 2?×?2?×?2?×?2?×?5 design: two soils (clayey Red Latosol and a sandy-loam Red-Yellow Latosol), two organic acids (citric acid, CA, and humic acid, HA), and two phosphorus (P) sources [monopotassium phosphate (KH₂PO₄) and inositol hexaphosphate (IHP)] either with or without heating (100 °C) of the soil. The soil plus treatments, in all combinations, were placed in contact with an anion exchange resin and the P in the resin was measured after 2, 4, 6, 10, and 15 days. The DFP was greater when the soils were treated with CA than when treated with HA. Citric acid was more effective in increasing the DFP from KH₂PO₄, whereas HA was the organic ligand that promoted a larger DFP when the source was IHP.     

Interactive effects of organic acids in the rhizosphere

Organic acids released into the rhizosphere may perform many beneficial functions to the plant including metal detoxification and enhancement of nutrient acquisition. Typically, these organic acids are studied in isolation; however, roots simultaneously exude a cocktail of organic acids and other substances, and their combined impact on rhizosphere processes may be quite different. It has been hypothesized that some exudates may play secondary roles (e.g. inhibitors of microbial activity, blockage of sorption sites), which might enhance the longevity and nutrient-mobilization capacity of others. Here we investigated how the decomposition, sorption and P-solubilizing effects of citrate, malate and oxalate are affected by the presence of malonate and shikimate. We found that in a range of agricultural soils the decomposition of citrate, malate and oxalate was rapid, but not influenced by the presence of large quantities of shikimate or malonate. This suggests that the individual organic acids are taken up by different transport mechanisms or components of the microbial community. At large concentrations, malonate decreased sorption of citrate, malate and oxalate on the soil, whilst shikimate had little effect. The capacity of citrate, malate and oxalate to desorb P was significantly greater in cocktails containing malonate compared with the single organic acid; no effect was seen with shikimate. We conclude that neither malonate nor shikimate at realistic concentrations will significantly affect the biodegradation of citrate, malate or oxalate in the rhizosphere, and while malonate did enhance P desorption, this effect is additive rather than synergistic. Overall, we found little evidence that malonate and shikimate act as secondary regulators of citrate, malate and oxalate behavior in soil.     

Low pH and aluminum tolerance of Bradyrhizobium strains isolated from acid soils in Indonesia

Sixty-seven strains of Bradyrhizobium isolated from soybean plants growing on acid soils in West Java and Sumatra, Indonesia, were examined for the effect of the pH and aluminum concentration on their growth in nutrient media, compared with 61 strains of Bradyrhizobium from soils in Japan. The results in this study indicated that the indigenous population of Bradyrhizobium in the soils of Indonesia showed a large difference in acid- and Al-tolerance from that of Japan. Eighty-five and 48% of the isolates from Japanese soils and Indonesian soils, respectively, were unable to grow in YEM broth at pH below 4.5. The acid-tolerance was correlated with AI-tolerance of the isolates on YEM agar plates at pH 4.4. Seventy-five percent of the isolates that grew in YEM broth at pH 4.5 were also resistant to 400 µM Al on the YEM plates. Acetylene reduction assay of the root nodules revealed that 3 of the acid- and Al-tolerant isolates from Indonesian soils showed a significantly high nitrogen fixation activity.     

Effect of humic acid and fulvic acid based liquid and foliar fertilizers on the yield of wheat crop

Abstract

An organic-mineral liquid fertigation fertilizer containing humic acid, nitrogen (N)–phosphorus (P)–potassium (K), zinc (Zn), sulfur (S), manganese (Mn) and a liquid foliar fertilizer consisting of fulvic acid and gibberellic acids were formulated and applied to wheat. The purpose of this study was to develop an efficient and cost effective liquid and foliar fertilizer and compare their potential with commercially available urea and DAP. The fulvic acid and humic acids were obtained by alkaline extraction of lignite coal. All the treatments were significant over the control. The application of the liquid fertigation fertilizer and liquid foliar fertilizer along with 50?kg of urea per acre showed the best results biological yield (grain plus straw yield). It was found that the highest yield was obtained in T₆ treatment and the second best results were obtained with T₂ treatment, but has a high cost, while treatment T₆ was the most significant economical and yielded a high income when compared to other treatments.     

Plant defence inducers rapidly influence the diversity of bacterial communities in a potting mix

Plant hormones and their functional analogues are used in agriculture to combat plant diseases and pests. These chemicals are generally targeted at shoots, but soils inevitably receive a dose during application. In this study, we used 16S rRNA gene amplicon pyrosequencing to determine whether the diversity of bacterial communities in a potting mix (referred here as ‘model soil’) is influenced by exposure to salicylic acid (SA), methyl jasmonate (MeJA), ethylene (ET) and abscisic acid (ABA). The number of operational taxonomic units (OTUs; richness) and Simpson's Diversity Index values (evenness) associated with each sample was not influenced by the hormone treatments, but changes in community composition were observed. Relative to the control, all hormones more than doubled the abundance of a Limnobacter population, ABA led to a four-fold increase in a Cellvibrio population, ET led to a c. 70% decrease in an Algoriphagus population, and MeJA and ABA applications halved the abundance of a Massilia population. These changes may influence plant productivity.     

不同来源腐殖酸的组成和性质的研究

本研究从三种有机物料草炭、褐煤、风化煤中提取腐殖酸,对其组成和性质进行了测定,同时对胡敏酸进行酒精沉淀分级。研究结果表明:褐煤胡敏酸氧化度和芳香度最高,其次为风化煤胡敏酸,最后是草炭胡敏酸;风化煤富里酸的氧化度和芳香度最高,其次为褐煤富里酸和草炭富里酸。胡敏酸各级分的回收率均随着级分数的升高而增加。回收率以风化煤胡敏酸最高,依次为褐煤胡敏酸、草炭胡敏酸。     

Chemical Composition of Ten Blackcurrant (Ribes nigrum L.) Cultivars

Abstract

Ten blackcurrant cultivars were evaluated for some quality components. On average, the sugar content was 9.0%, of which fructose made up 45%, glucose 40% and sucrose 15%. Soluble solids were 15.4% as an average for the ten cultivars in three years. The acid content was 5.1% of fresh weight, 88% was citric acid and 12% malic acid. The ascorbic acid content varied from 67 mg per 100 g fresh fruit in Hedda, to 204 mg per 100 g in Blackdown. Except for Hedda and Øjebyn, which both had low values of ascorbic acid and colour properties, the quality components of the cultivars were found to be within an acceptable range.     

Soil Humic and Fulvic Acids from Different Land-Use Systems Evaluated By E4/E6 Ratios

Changes in land-use systems such as the introduction of trees on agricultural land can give rise to changes in the physicochemical properties of the soils, also affecting the quantity and quality of organic matter incorporated into the system. The present study assesses humic substances (HS) in the soil such as humic acid (HA) and fulvic acid (FA) by looking at the relationship between the optic densities determined at 465 and 665 nm (E4/E6 ratio). Topsoil samples (0–20 cm) from pine woodlands 60 years of age were compared with agricultural soils of similar age in the central-south region of the Province of Buenos Aires, Argentina. The pH of the topsoil from beneath the pine trees was highly acidic (5.0 vs. 6.2) and a significant increase in the level of organic carbon (OC) was registered. The carbon to nitrogen (C/N) ratio was also higher (by one order of magnitude) beneath the pine trees, although the humification conditions of the soil organic matter (SOM) were good in the soils of both studied land-use systems. The E4/E6 ratio was higher in the HA and FA 2º (second extraction) beneath the pines, indicating a smaller molecular size of the HS bound to the clay minerals. This fact can be attributed to the higher concentration of hydrogen ions beneath the pines and consequently the loss of polyvalent cations, mainly calcium. The most labile organic molecules (FA 1º – first extraction) were of a larger size in soils beneath the pines, most likely owing to a specific characteristic of the Pinus genus, although the fraction in question constitutes a minority fraction among the HS. Clear differences were established between the E4/E6 ratios in HA and FA, making this a highly useful method for determining molecular changes in HS as a result of changes in land use.