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:用8个微卫星标记对闽南黄牛的等位基因频率、多态信息含量、杂合度和有效等位基因数等指标进行统计分析,并在此基础上对其进行聚类分析和分类研究。结果:8个微卫星标记在所检测的闽南黄牛群体中都表现出较丰富的多态性,在4个群体8个微卫星座位共有58个等位基因,每个微卫星标记平均检测到7.2个等位基因(3~11);8个微卫星标记的平均多态信息含量(PIC)为0.6471,各群体的平均多态信息含量(PIC)差并不显著;全部群体平均杂合度为0.2930;各群体平均有效等位基因数为3.37。此表明闽南黄牛在微卫星位点上具有丰富的遗传多样性;根据奈氏标准遗传距离进行UPGMA聚类分析,结果表明4个群体间的遗传变异不大。 相似文献
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试验旨在分析岷县黑裘皮羊群体内遗传多样性,筛选出理想的遗传标记,为岷县黑裘皮羊的选育保种提供理论依据。选取144只岷县黑裘皮羊,颈静脉采血并提取DNA,对24对微卫星引物进行PCR扩增,用毛细管电泳技术进行基因分型,计算其等位基因数、等位基因大小及频率、有效等位基因数、杂合度和多态信息含量。结果显示,24个位点共检测到210个等位基因,平均等位基因数为8.75;群体等位基因频率为0.0104~0.7396,等位基因片段大小为97~285 bp;有效等位基因数为1.7581~8.2433个;群体平均观测杂合度为0.4839;平均期望杂合度为0.6959;平均多态信息含量为0.6527。其中MAF70位点等位基因数、有效等位基因数、期望杂合度和多态信息含量最高;OarFCB128位点观测杂合度最高;OarAE129位点等位基因数最少,SRCRSP9位点期望杂合度、多态信息含量最低;OarFCB304位点观测杂合度最低。Hardy-Weinberg平衡分析结果表明,所选位点中有19个处于非平衡状态。结果表明,岷县黑裘皮羊的遗传背景复杂,群体内遗传多样性丰富,可为其遗传资源的评估和选育保种工作提供理论依据。 相似文献
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试验旨在分析岷县黑裘皮羊群体内遗传多样性,筛选出理想的遗传标记,为岷县黑裘皮羊的选育保种提供理论依据。选取144只岷县黑裘皮羊,颈静脉采血并提取DNA,对24对微卫星引物进行PCR扩增,用毛细管电泳技术进行基因分型,计算其等位基因数、等位基因大小及频率、有效等位基因数、杂合度和多态信息含量。结果显示,24个位点共检测到210个等位基因,平均等位基因数为8.75;群体等位基因频率为0.0104~0.7396,等位基因片段大小为97~285 bp;有效等位基因数为1.7581~8.2433个;群体平均观测杂合度为0.4839;平均期望杂合度为0.6959;平均多态信息含量为0.6527。其中MAF70位点等位基因数、有效等位基因数、期望杂合度和多态信息含量最高;OarFCB128位点观测杂合度最高;OarAE129位点等位基因数最少,SRCRSP9位点期望杂合度、多态信息含量最低;OarFCB304位点观测杂合度最低。Hardy-Weinberg平衡分析结果表明,所选位点中有19个处于非平衡状态。结果表明,岷县黑裘皮羊的遗传背景复杂,群体内遗传多样性丰富,可为其遗传资源的评估和选育保种工作提供理论依据。 相似文献
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《中国养兔杂志》2015,(6)
为了从DNA分子水平揭示苏系长毛兔遗传多态性和遗传结构,选用6个多态性较好的微卫星标记,对96只宜兴拉必得种兔场发育良好的苏系长毛兔群体进行了检测分析。利用等位基因频率计算苏系长毛兔群体的期望杂合度(He)、多态信息含量(PIC)、等位基因数、有效等位基因数。6个微卫星座位全部呈现出高度多态性(PIC0.5),共检测到36个等位基因,每个座位的等位基因数在5~7个之间,平均等位基因数6.00±0.8944个,平均有效等位基因数为3.74±0.8159,该长毛兔群体期望杂合度(He)和多态信息含量(PIC)分别为0.7257±0.0589和0.6758±0.0721。研究结果表明:苏系长毛兔的微卫星座位有丰富的遗传多样性。通过选育可以使该苏系长毛兔群体进一步纯化,从而使其生产性能稳定。 相似文献
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陇东绒山羊微卫星DNA遗传多样性的研究 总被引:2,自引:1,他引:1
利用9个微卫星座位,8%非变性聚丙烯酰胺凝胶电泳PCR扩增产物,对120只陇东绒山羊基因组DNA的遗传多样性进行了检测,对其群体遗传学特性进行分析。结果表明:陇东绒山羊在9个微卫星座位中共检测到90个等位基因,其中微卫星座位LSCV24等位基因数最多,为14个;BMS1248多态信息含量最高,PIC为0.88;DB6座位多态信息含量最低,PIC为0.79。在所标记群体中平均有效等位基因数为7.69±1.65个,平均多态信息含量为0.84±0.03;遗传杂合度均值为0.86±0.03,均高于国内外部分研究结果。说明陇东绒山羊品种遗传多样性丰富,群体遗传变异程度高,育种潜力大。 相似文献
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选取10个微卫星位点,结合荧光标记(FAM) PCR扩增技术,运用毛细管电泳检测扩增产物,利用Microsatellite Toolkit、PopGene软件计算遗传相关指标,探讨广西树鼩群体的遗传多态性。结果发现,在10个微卫星基因座中,共检测到64个等位基因,平均等位基因数为6.4个,平均有效等位基因数为3.7892,平均观察杂合度和平均期望杂合度为0.6156和0.6963,平均多态信息含量为0.6367,其中7个是高度多态位点,3个属中度多态位点。此外,在10个微卫星基因座中,有5个位点偏离Hardy-Weinberg平衡(P<0.01)。结果表明广西树鼩遗传多态性较丰富,所选微卫星位点基本可用于评估广西树鼩群体遗传多样性。 相似文献
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为了对摩拉水牛、尼里-拉菲水牛进行合理有效的保护,研究其遗传多样性,掌握其遗传背景,试验利用15个微卫星位点对96个个体(尼里-拉菲、摩拉水牛各48头)进行遗传多样性检测。结果表明:在尼里-拉菲水牛15个微卫星位点上共检测到92个等位基因,平均等位基因为6. 133 3,平均有效等位基因为2. 942 3,平均多态信息含量(PIC)为0. 565 5,平均期望杂合度值为0. 618 4;在摩拉水牛15个微卫星位点上共检测到76个等位基因,平均等位基因为5. 133 3,平均有效等位基因为2. 545 4,平均PIC为0. 493 7,平均期望杂合度值为0. 541 3。说明摩拉水牛、尼里-拉菲水牛群体的遗传多样性比较丰富,尼里-拉菲水牛群体的遗传多样性高于摩拉水牛群体。 相似文献
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魏静 《四川畜牧兽医学院学报》2009,(4):28-32
在现代法律秩序中,商会自治规范是制定法的基础和必要的补充,甚至在某些方面替代了制定法;商会自治规范主要包括商会组织规范、行为规范、惩罚规范以及争端解决规范等;其效力仅及于其内部成员;商会自治规范和制定法之间存在冲突,但也存在整合的基础。 相似文献
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本文概述了猪的毛色类型、猪的毛色遗传模式,着重综述了猪毛色基因分子基础的研究进展,指出存在问题并就未来发展方向做了思考。 相似文献
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以国际标准强毒R株人工感染非免疫产蛋鸡,定时扑杀,分别从鼻窦、眶下孔、气管、肺、气囊、卵巢和输卵管分离MG,并收集感染鸡所产蛋分离MG。结果表明,人工感染48小时后上、下呼吸道及肺已被全面感染,96小时气囊已被感染,120小时输卵管已能分离到MG,卵巢始终分离不到MG。人工感染鸡自144小时便能在其所产蛋中分离出MG。药物治疗能在72小时内消除感染,油乳剂苗则需24天后逐渐降低蛋内MG分离率,药物卵内注射、种蛋药浴、高温处理均能杀死卵内MG,但以研制的种蛋浸泡剂药浴效果为最好。 相似文献
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REASONS FOR PERFORMING STUDY: Centesis of the bicipital bursa using an 8.9 cm long spinal needle has been reported but the alternative of employing a 3.8 cm long hypodermic needle requires validation. OBJECTIVE: To compare the efficacy of 2 different methods of centesis of the bicipital bursa and to evaluate the usefulness of ultrasonographic imaging to determine the location of solution administered when centesis of the bursa is attempted. METHODS: For Trial 1, 6 clinicians, who had no previous experience of centesis of the bicipital bursa, attempted to inject a solution composed of an aqueous radiopaque contrast medium and physiological saline solution (PSS) into the bicipital bursae of 2/12 horses using the previously described distal approach to inject one bursa and a proximal approach to inject the contralateral bursa. The bicipital tendon and bursa were examined ultrasonographically before and after injection; and both shoulders were examined radiographically to identify the location of the medium. In Trial 2, another 6 clinicians, also with no previous experience of centesis, repeated Trial 1, using 6 horses, but the radiopaque contrast medium was mixed with air instead of PSS. RESULTS: Accuracy of centesis using the proximal approach was 39% and that of the distal approach 28%. Ultrasonographic examination of the shoulder allowed the location of solution and air to be accurately predicted in all 12 shoulders examined. CONCLUSIONS: Clinicians who have had no previous experience performing centesis of the bicipital bursa are unlikely to be successful in centesis using either approach. Radiographic examination after injecting a radiopaque contrast medium may be necessary to assess the success of centesis especially if bursal fluid is not obtained during centesis. Injecting air along with the radiopaque contrast medium provides more accurate ultrasonographic confirmation of centesis and better radiographic definition than does injection without air. 相似文献
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Effects of size of ingestively masticated fragments of plant tissues on kinetics of digestion of NDF
Ingestively masticated fragments were collected and sized via sieving. Different sizes of esophageal masticate and ruminal digesta fragments, and ground fragments of larger masticated pieces were incubated in vitro, and undigested NDF remaining at intervals of up to 168 h of incubation was determined. The ruminal age-dependent time delay (tau) for onset of digestion of NDF was positively correlated (P < 0.004) with the mean sieve aperture estimated to retain 50% of the fragments between successive sieve apertures (MRA). Degradation rate of potentially degradable NDF (PDF) and level of indigestible NDF were not related (P > 0.10) to MRA of masticated and ground fragments. Estimates of tau were positively related to MRA, with slopes of bermudagrass < corn silage < ruminal fragments of corn silage. It was concluded that fragment size-, and consequently, ruminal age-dependent onset of PDF degradation of a mixture of different fragment sizes results in an age-dependent rate of degradation of the more rapidly degrading of two subentities of PDF. Models are proposed that assume a tau before onset of simultaneous degradation of PDF from two pools characterized as having gamma-modeled age-dependency and age-constant rates. The ruminal age-dependent pool seems to be associated with the faster-degrading pool, and its rate parameter increases with range in MRA in the population of fragments. Conceptually, the ruminal age-dependent rate parameter for PDF degradation seems to represent a composite of several effects: 1) effects of the size-dependent tau; 2) range in MRA of the population of ingestively masticated fragments; and 3) subentities of PDF that degrade via more rapid age-dependent rates compared with subentities of PDF that degrade via age-constant rates. The estimated fractional rates of ruminative comminution of ingestively masticated fragments (0.060 to 0.075/h) were of a magnitude similar to the mean fractional rates of PDF digestion (0.030 to 0.085/h), which implies that ruminative comminution may be first-limiting to fractional rate of PDF digestion. The in vivo roles of ingestive and ruminative mastication of fragments on PDF degradation must be considered in any kinetic system for estimating PDF digestion in the rumen. These results and others in the literature suggest that the rate of surface area exposure rather than intrinsic chemical attributes of PDF may be first-limiting to degradation rate of PDF in vivo. 相似文献