绵羊痘治疗方法研究

绵羊痘是急性热性接触性传染病,具有较强的传染性,所有绵羊均可感染,病死率较高,会对羊养殖业造成严重危害。基于此,文章从绵羊痘的症状入手,论述如何诊断绵羊痘,结合绵羊养殖实践,总结绵羊痘的治疗方案,尽早控制绵羊痘病情发展,避免绵羊痘传染范围扩大。     

山羊痘活疫苗防制绵羊痘病的应用

近几年 ,绵羊痘东北部分地区发生 ,我们针对周边地区绵羊痘病疫情 ,及时对存栏 1 2 0 0 0只绵羊进行了绵羊痘弱毒疫苗免疫注射 ,3个月后 ,个别羊群出现痘病零星散发 ,当时正值进入接羔期 ,初生羔羊 1 0天后开始发病 ,羔羊面临大批损失危险。对此 ,我们依据国内外文献资料 :山羊痘疫苗可坚强地抗山羊痘和绵羊痘 ;山羊痘活疫苗使用说明可用于绵羊 ,进行了山羊痘活疫苗免疫绵羊试验示范 ,通过观察免疫效果 ,与国内外资料报道一致 ,并且 ,山羊痘活疫苗免疫绵羊的效果明显优于绵羊痘弱毒疫苗。1　材料与方法1 .1　材料　山羊痘活疫苗 ,吉林省生物…     

春季谨防羊痘的发生

羊痘包括绵羊痘和山羊痘。侵害绵羊的为绵羊痘．侵害山羊的为山羊痘，山羊、绵羊互不传染，绵羊比山羊更容易感染。绵羊痘是由绵羊痘病毒引发。是多种家畜痘病中危害最严重的一种急性、热性、接触性传染病，具有典型病理过程．以在无毛或少毛的皮肤和黏膜上发生特征性痘疹为特征。山羊痘的病原为山羊痘病毒．该病较少见．其临床症状和病理变化与绵羊痘相似．但症状较轻．     

中药治疗绵羊痘

绵羊痘的病原体为绵羊痘病毒,该病是由绵羊痘病引起的一种急性、热性、接触性、发疹传染病。笔者采取中药治疗绵羊痘60多例,治愈率95%。1流行特点     

绵羊痘种毒保存期试验

鸡胚化绵羊痘羊体反应细胞毒(以下简称“绵羊痘种毒”)是制备绵羊痘活疫苗的基础种子，对于预防和控制绵羊痘疫情具有重要意义，定期对其检定十分必要。2000年版《中华人民共和国兽用生物制品规程》(以下简称“《规程》”)规定：绵羊痘种毒在-30℃以下，保存期为10年。为进一步探究绵羊痘种毒的保存期，本试验选取1981年1月、1983年1月、1990年4月和2006年2月冻干并于-70℃保存至今的4株绵羊痘种毒，进行病毒含量测定和最小发痘量测定。结果显示，4株绵羊痘种毒的最小发痘量均达到10^-5/0.5 mL,符合《规程》之规定；病毒含量分别为10^3.3、10^3.8、10^4.8和10^5.5 TCID₅₀/0.1 mL,且与最小发痘量在一定范围内呈正相关。结果表明，冻干绵羊痘种毒在-70℃条件下保存39年仍符合生产活疫苗的免疫原性标准，因此可将绵羊痘种毒的保存期延长至-70℃保存30年，本试验为绵羊痘种毒的保藏检定和疫苗生产提供数据支撑。     

绵羊痘的诊断与防治

绵羊痘是绵羊的主要疫病之一,有很强的传染性,给养羊户带来巨大损失。本文通过对本地区绵羊痘病的流行特点、临床症状、免疫预防和临床治疗方案等进行总结,为提高广大养羊户和基层专业技术人员对绵羊痘病基本知识的了解和危害性的认识,从而为绵羊痘病的综合防治提供参考。     

绵羊痘病的诊治

以一起绵羊痘病的诊治过程为例,介绍了该病的病因、临床症状、病理变化以及中西医结合治疗流行性绵羊痘病的方法。并提出加强饲养管理、做好预防消毒工作是有效防控绵羊痘病的关键。     

绵羊痘病的诊治

以一起绵羊痘病的诊治过程为例,介绍了该病的病因、临床症状、病理变化以及中西医结合治疗流行性绵羊痘病的方法。并提出加强饲养管理、做好预防消毒工作是有效防控绵羊痘病的关键。     

绵羊痘的PCR检测与诊断

绵羊痘是痘病的其中之一,也是世界动物卫生组织通报和跨界的重要羊病之一,给世界养羊业带来巨大的经济损失。绵羊痘的主要特征是在绵羊的皮肤和黏膜上形成痘疹。本研究根据数据库中最新发表的绵羊痘病毒P32基因序列,设计并合成了1对能特异性扩增绵羊痘病毒基因片段的特异性引物序列,建立了检测与诊断绵羊痘的PCR方法,扩增产物大小为500 bp。结果显示,绵羊痘均能扩增出条带,其他健康羊只均不能扩增出条带。应用本研究建立的方法对临床样本进行检测与诊断,10份临床疑是病料中6份均可扩增出500 bp的绵羊痘病毒特异性片段。该方法可用来绵羊痘的诊断、检测、预防控制及流行病学调查。     

羊痘的诊断与防治

正羊痘包括绵羊痘和山羊痘,绵羊痘是各种家畜痘病中危害最为严重的一种急性、热性、接触性传染病,其特征是病羊皮肤和黏膜出现特异性的痘疹;山羊痘较少见,常侵害个别羊群,传染慢,症状和病变与绵羊痘相似,病势较轻。加强饲养管理,采取严格引进措施和防检疫措施,可有效防止疫病发生。1病原病原为痘病毒科的绵羊痘病毒和山羊痘病毒,山羊痘是山羊痘病毒引进的一种急性接触性传染     

癸氧喹酯干混悬剂含量测定的改进

采用高效液相色谱法测定癸氧喹酯干混悬剂的含量,在2-250μg/mL范围内,峰面积的常用对数与进样量浓度的常用对数呈良好的线性关系,R^2=1(n=5),平均回收率为99.24%~99.51%,RSD在0.05%~0.28%。此方法分析时间短,样品前处理简便、定量结果准确,重现性好,结果满意,为其质量控制提供了依据。     

商会自治的基石——商会自治规范研究

在现代法律秩序中,商会自治规范是制定法的基础和必要的补充,甚至在某些方面替代了制定法;商会自治规范主要包括商会组织规范、行为规范、惩罚规范以及争端解决规范等;其效力仅及于其内部成员;商会自治规范和制定法之间存在冲突,但也存在整合的基础。     

猪毛色遗传的研究进展

本文概述了猪的毛色类型、猪的毛色遗传模式,着重综述了猪毛色基因分子基础的研究进展,指出存在问题并就未来发展方向做了思考。     

中华人民共和国农业部公告 第267号

为贯彻落实《兽药生产质量管理规范》(简称《兽药GMP》),进一步推动兽药GMP实施进程,我部制定了《兽药生产质量管理规范检查验收办法》,现予公告。本公告自2003年6月1日起施行。附件:兽药生产质量管理规范检查验收办法二○○三年四月十日第一章 总则 第一条 为推动《兽药生产质量管理规范》(以下简称兽药GMP)的实施,规范兽药GMP检查验收工作,制定本办法。 第二条 农业部负责全国兽药GMP管理和检查验收工作;负责制修订兽药GMP检查验收管理规定;负责兽药GMP检查员队伍建设和监督管理工作,负责国际兽药贸易中GMP互认工作。 …     

鸡败血支原体垂直传播模式及其阻断方法

以国际标准强毒Ｒ株人工感染非免疫产蛋鸡,定时扑杀,分别从鼻窦、眶下孔、气管、肺、气囊、卵巢和输卵管分离ＭＧ,并收集感染鸡所产蛋分离ＭＧ。结果表明,人工感染４８小时后上、下呼吸道及肺已被全面感染,９６小时气囊已被感染,１２０小时输卵管已能分离到ＭＧ,卵巢始终分离不到ＭＧ。人工感染鸡自１４４小时便能在其所产蛋中分离出ＭＧ。药物治疗能在７２小时内消除感染,油乳剂苗则需２４天后逐渐降低蛋内ＭＧ分离率,药物卵内注射、种蛋药浴、高温处理均能杀死卵内ＭＧ,但以研制的种蛋浸泡剂药浴效果为最好。     

Comparison of 2 methods of centesis of the bursa of the biceps brachii tendon of horses

REASONS FOR PERFORMING STUDY: Centesis of the bicipital bursa using an 8.9 cm long spinal needle has been reported but the alternative of employing a 3.8 cm long hypodermic needle requires validation. OBJECTIVE: To compare the efficacy of 2 different methods of centesis of the bicipital bursa and to evaluate the usefulness of ultrasonographic imaging to determine the location of solution administered when centesis of the bursa is attempted. METHODS: For Trial 1, 6 clinicians, who had no previous experience of centesis of the bicipital bursa, attempted to inject a solution composed of an aqueous radiopaque contrast medium and physiological saline solution (PSS) into the bicipital bursae of 2/12 horses using the previously described distal approach to inject one bursa and a proximal approach to inject the contralateral bursa. The bicipital tendon and bursa were examined ultrasonographically before and after injection; and both shoulders were examined radiographically to identify the location of the medium. In Trial 2, another 6 clinicians, also with no previous experience of centesis, repeated Trial 1, using 6 horses, but the radiopaque contrast medium was mixed with air instead of PSS. RESULTS: Accuracy of centesis using the proximal approach was 39% and that of the distal approach 28%. Ultrasonographic examination of the shoulder allowed the location of solution and air to be accurately predicted in all 12 shoulders examined. CONCLUSIONS: Clinicians who have had no previous experience performing centesis of the bicipital bursa are unlikely to be successful in centesis using either approach. Radiographic examination after injecting a radiopaque contrast medium may be necessary to assess the success of centesis especially if bursal fluid is not obtained during centesis. Injecting air along with the radiopaque contrast medium provides more accurate ultrasonographic confirmation of centesis and better radiographic definition than does injection without air.     

不同样本商品蜂蜜中硒含量的测定

用硝酸和高氯酸消化蜂蜜,使硒游离出来,在微酸性环境下,硒和2,3-二氨基萘(DAN)生成有较强荧光的物质,用环己烷萃取,在激发波长378nm,荧光波长518nm处测定其荧光强度。蜂蜜中硒含量范围:0.10～0.82μg/g。表明:蜂蜜应视为天然富硒营养品。     

乳酸杆菌益生作用机制的研究进展

乳酸杆菌作为益生菌广泛用于人和动物。本文综述了乳酸杆菌改善宿主健康的机制。乳酸杆菌可通过产生抗菌物质如乳酸、过氧化氢、细菌素,或者通过竞争营养或肠道黏附位点来抑制致病菌;通过诱导黏附素的分泌或阻止细胞凋亡而增强肠道的屏障功能,从而保护肠道。文章重点讨论了乳酸杆菌表面成分（表面蛋白、脂磷壁酸和肽聚糖）与肠道受体（Ｃ型凝集素受体、Ｔｏｌｌ样受体和 Ｎｏｄ样受体）,阐述了他们结合后启动免疫调节信号,调控肠道免疫功能以发挥改善健康作用的机制。     

Effects of size of ingestively masticated fragments of plant tissues on kinetics of digestion of NDF

Ingestively masticated fragments were collected and sized via sieving. Different sizes of esophageal masticate and ruminal digesta fragments, and ground fragments of larger masticated pieces were incubated in vitro, and undigested NDF remaining at intervals of up to 168 h of incubation was determined. The ruminal age-dependent time delay (tau) for onset of digestion of NDF was positively correlated (P < 0.004) with the mean sieve aperture estimated to retain 50% of the fragments between successive sieve apertures (MRA). Degradation rate of potentially degradable NDF (PDF) and level of indigestible NDF were not related (P > 0.10) to MRA of masticated and ground fragments. Estimates of tau were positively related to MRA, with slopes of bermudagrass < corn silage < ruminal fragments of corn silage. It was concluded that fragment size-, and consequently, ruminal age-dependent onset of PDF degradation of a mixture of different fragment sizes results in an age-dependent rate of degradation of the more rapidly degrading of two subentities of PDF. Models are proposed that assume a tau before onset of simultaneous degradation of PDF from two pools characterized as having gamma-modeled age-dependency and age-constant rates. The ruminal age-dependent pool seems to be associated with the faster-degrading pool, and its rate parameter increases with range in MRA in the population of fragments. Conceptually, the ruminal age-dependent rate parameter for PDF degradation seems to represent a composite of several effects: 1) effects of the size-dependent tau; 2) range in MRA of the population of ingestively masticated fragments; and 3) subentities of PDF that degrade via more rapid age-dependent rates compared with subentities of PDF that degrade via age-constant rates. The estimated fractional rates of ruminative comminution of ingestively masticated fragments (0.060 to 0.075/h) were of a magnitude similar to the mean fractional rates of PDF digestion (0.030 to 0.085/h), which implies that ruminative comminution may be first-limiting to fractional rate of PDF digestion. The in vivo roles of ingestive and ruminative mastication of fragments on PDF degradation must be considered in any kinetic system for estimating PDF digestion in the rumen. These results and others in the literature suggest that the rate of surface area exposure rather than intrinsic chemical attributes of PDF may be first-limiting to degradation rate of PDF in vivo.