冷冻稀释液中添加大豆卵磷脂对梅花鹿冻融精子质量的影响

【目的】 探究在冷冻稀释液中添加大豆卵磷脂代替10%卵黄对梅花鹿精液冷冻保存效果的影响,为梅花鹿人工授精体系的完善提供参考。【方法】 采用电刺激法采集梅花鹿精液,以精液冷冻稀释液中分别添加1%、2%、3%、4%和5%大豆卵磷脂代替10%卵黄作为试验组,添加20%卵黄作为对照组,分别进行各组精液冷冻保存。5 d后,进行精液解冻,检测解冻后各组精子的活力、质膜完整率、顶体完整率、线粒体活性、存活时间,筛选合适浓度的大豆卵磷脂。选取4~5岁健康雌性梅花鹿,肌肉注射300 IU孕马血清促性腺激素（PMSG）和0.4 mg氯前列醇钠进行同期发情处理,发情后第20 h用20%卵黄组与筛选出的大豆卵磷脂组冻精进行人工输精,输精后30 d使用B超检测仪检测妊娠情况,统计妊娠率。【结果】 与对照组相比,1%大豆卵磷脂组冻融后的精子活力、向前活动力、快速前进活力、活率、质膜完整率、顶体完整率及线粒体活性均显著提高（P<0.05）;随着稀释液中大豆卵磷脂浓度的增加,其冻融后精子活力、向前活动力、快速前进活力、活率、质膜完整率、顶体完整率以及线粒体活性呈下降趋势,精子存活时间也随浓度的增加而减少。1%大豆卵磷脂组冻融精子人工授精梅花鹿的妊娠率为61.11%,高于对照组、2%和3%大豆卵磷脂组,但差异均不显著（P>0.05）。【结论】 在梅花鹿精子冷冻稀释液中添加1%大豆卵磷脂替代10%卵黄,能有效提高梅花鹿冻融精子的质量,为进一步筛选新型梅花鹿精液冷冻稀释液提供理论基础。     

冷冻保护剂对家畜精液冷冻保存的研究进展

家畜精液冷冻保存可以实现在不同时间、不同地点的人工授精,能有效提高优良种公畜的利用率,扩大其基因传播范围,同时也是家畜遗传资源易位保存的主要方法之一。在精液冷冻保存过程中,选择合适的稀释液成分对解冻后精子品质的影响十分重要。精子对低温非常敏感,在稀释液中加入冷冻保护剂可有效提升精子解冻后活力。文章综述了近几年渗透性冷冻保护剂(甘油、乙二醇、二甲基甲酰胺、二甲基乙酰胺、二甲基亚砜)和非渗透性冷冻保护剂(卵黄、大豆卵磷脂、蔗糖、脱脂乳)在猪、牛、羊、鸡、兔以及骆驼上的应用与研究,旨在为进一步深入研究家畜精子冻融损伤机制提供理论依据。     

解冻稀释液中添加精浆对冻融猪精子品质的影响

【目的】 探究冷冻前添加热休克蛋白A8(heat shock protein A8, HSPA8)和解冻后添加不同浓度精浆(seminal plasma, SP)对冻融猪精子的影响。【方法】 采用手握法采集长白猪精液, 添加0.5 μg/mL HSPA8到猪精液冷冻保护剂中进行细管分装, 投入液氮中保存3周后进行解冻, 解冻后添加不同浓度精浆(0、10%、30%和50%), 对冻融后长白猪精子的运动能力、质膜完整性、顶体完整性、细胞凋亡、线粒体膜电位、鱼精蛋白缺乏及体外获能水平等进行评估。【结果】 与对照组相比(无HSPA8和精浆), 添加0.5 μg/mL HSPA8处理组(无精浆)的精子直线速度(VSL)、曲线速度(VCL)、平均路径速度(VAP)和前向性运动(STR)均显著提升(P<0.05), 精子直线性运动(LIN)和运动的摆动性(WOB)均无显著差异(P>0.05);精子质量参数中活力、质膜完整性和顶体完整性均显著升高(P<0.05), 细胞凋亡水平与线粒体膜电位均显著降低(P<0.05);精子鱼精蛋白缺失率显著降低(P<0.05);精子蛋白酪氨酸磷酸化水平显著提高(P<0.05)。之后在解冻液中添加不同浓度的精浆, 与添加0.5 μg/mL HSPA8处理组(无精浆)相比, 精浆添加量达到50%时, 精子VSL、VCL、VAP、LIN、STR和WOB均显著提升(P<0.05);精子活力、质膜完整性、顶体完整性和线粒体膜电位均显著提高(P<0.05), 细胞凋亡水平显著降低(P<0.05);精子鱼精蛋白缺失率显著降低(P<0.05);精子蛋白酪氨酸磷酸化水平显著提高(P<0.05)。【结论】 在冷冻基础液中添加0.5 μg/mL HSPA8和解冻稀释液中添加50%精浆联合使用可以有效改善冻融精子质量, 将会对猪精液的冷冻保存及商业化生产提供一定的参考。     

精浆对猪精子冷冻效果影响的研究

为优化猪精子冷冻技术,提高解冻后精子的活力和受精能力,本试验分别以含精浆浓度为10%、20%和30%的冷冻保护剂处理精子,以冷冻前、解冻后精子活力和质膜完整性,解冻后精子进行体外受精(IVF)的卵裂率和囊胚率等作为检测指标,同时以含有卵黄的Tris-柠檬酸－葡萄糖(Tris-citric acid-glucose,TCG)冷冻基础液作为对照研究精浆对猪冷冻精子的保护作用。结果显示,在含有10%精浆浓度的稀释液中,冷冻前质膜完整性,解冻后精子活率、质膜完整性、IVF囊胚率相对于对照组均显著提高(P<0.05);当含有10%精浆的冷冻精液解冻后用于人工授精时,与配母猪妊娠率、窝产仔数、窝产活仔数等仍显著低于鲜精授精组(P<0.05)。上述结果表明,含10%精浆的冷冻保护剂能提高精子的冷冻后活力和IVF胚胎发育率,但用于人工授精配种与鲜精相比还有一定差距。     

不同浓度大豆卵磷脂替代蛋黄对东佛里生奶绵羊精液冷冻保存效果的影响

本试验皆在研究添加不同浓度大豆卵磷脂（SL）冷冻保存东佛里生奶绵羊精液的效果。我们在Tris基础稀释液中,添加18%蛋黄为对照组,添加0.5%、1%、1.5%、2%、2.5%SL设为试验组,检测冷冻精液解冻后的精子活率和顶体完整率。结果显示,添加0.5%、2.5% SL冷冻稀释液稀释的精液,解冻后精子活率和顶体完整率与其他组之间存在显著差异（P<0.05）;添加18%蛋黄和1%～2% SL冷冻稀释液稀释的精液,冷冻解冻后精子活率和顶体完整率之间无显著差异（P>0.05）;添加18%蛋黄和1.0%～1.5% SL冷冻稀释液稀释后的精液,进行人工授精后母羊的妊娠率与对照组无显著差异（P>0.05）。因此,大豆卵磷脂可以作为冷冻保护剂用于东佛里生奶绵羊精液的冷冻保存,其最佳添加浓度为1～2%（g／L）。     

不同冷冻保护液和解冻温度对猪精液冷冻效果的影响

在BF5稀释液中分别添加不同水平的牛血清白蛋白(BSA)(0.5,1,1.5 g/L)、二甲基乙酰胺(DMA)(0.5%,1%,1.5%)、甲基-β-环糊精载胆固醇(CLC)(1,2.5,5 g/L),对成年健康猪精液进行冷冻保存,于不同温度(37,50,70℃)解冻后分别检测冷冻后精子的活率、活力、质膜完整性、项体完整率、线粒体活性.结果显示,0.5 g/L BSA组冷冻后精子活力、质膜完整率、顶体完整率和线粒体活性均高于其他组,但差异不显著(P0.05).1% DMA组冷冻后精子活率和活力显著优于1.5%DMA组(P＜0.05),同时也高于对照组(3%甘油)冻后精子活率和活力,但差异不显著.不同质量浓度CLC组冷冻后精子的活率、活力、质膜完整性和线粒体活性与对照组相比差异不显著.50℃和70℃解冻组精子的活率和线粒体活性显著高于37℃解冻组的精子;50℃解冻组精子的活力和质膜完整率显著高于其他2组.因此,在猪精液冷冻稀释液中,用DMA可以代替甘油作为渗透性保护剂,且以1%DMA,50℃解冻最佳.     

牛血清白蛋白对犬精液冷冻保存效果的影响

为了研究牛血清白蛋白(BSA)对犬精液冷冻保存效果的影响,试验配制含有不同浓度(0、3%、5%、7%)BSA的精液冷冻稀释液用于保存犬精液,测定冷冻-解冻后精子活力、质膜完整率、顶体完整率、总抗氧化能力(T-AOC)、丙二醛(MDA)含量及线粒体膜电位(MMP)等指标。结果表明：3%和5%BSA组冷冻-解冻后精子活力、质膜完整率、顶体完整率和T-AOC均显著高于对照组(P<0.05);3%BSA组MDA含量显著低于其他各组(P<0.05),线粒体膜电位值显著高于其他各组(P<0.05);除精子活力外,7%BSA组和对照组之间各项指标均差异不显著(P>0.05)。说明在犬精液冷冻稀释液中添加BSA能有效改善冷冻-解冻后精液品质,对提高精子抗氧化能力及代谢能力都有显著效果,BSA的最适添加浓度为3%。     

大豆卵磷脂与卵黄在山羊精液冷冻过程中的联合作用研究

试验旨在探究在山羊精液冷冻保存过程中单独使用大豆卵磷脂(SL)、卵黄(EY)及其联合使用的效果。以20%EY(V/V)和2%SL(m/V)为对照,在山羊冻精基础稀释液中分别添加10%EY+2%SL、20%EY+1%SL、20%EY+2%SL,冷冻解冻后分别对精子活率、精子活力、质膜完整率、顶体完整率和线粒体活性进行检测,同时利用试剂盒检测活性氧(ROS)、丙二醛(MDA)、超氧化物歧化酶(SOD)含量变化。结果表明:当冷冻稀释液中联合添加10%EY+2%SL,解冻后该组的精子活力、精子活率、质膜完整率、顶体完整率和线粒体活性与20%EY和2%SL对照组无显著性差异,较20%EY+1%SL和20%EY+2%SL联合添加组显著提高;与20%EY和2%SL组相比,联合添加10%EY+2%SL组精子解冻后ROS和MDA含量和SOD活性无显著性差异,但精子抗氧化能力较另外2个联合添加组显著提高。综上,山羊精液冷冻基础稀释液中添加SL可替代或者部分替代EY,EY和SL的联合添加对山羊冻精无协同作用,20%EY联合添加不同浓度的SL则会加大对冷冻精子的氧化损伤。     

辅酶Q10对绒山羊精液冷冻保存效果的影响

旨在探讨辅酶Q10对绒山羊精液冷冻保存效果的影响。利用添加不同浓度辅酶Q10(4、40、400?滋g/mL)的精液冷冻稀释液对绒山羊精液样本进行冷冻保存,待冷冻精液解冻后,采用流式细胞仪和计算机辅助精液分析系统(CASAS)分别检测不同精液样本的精子活率、质膜完整率、顶体完整率、DNA完整率、线粒体膜电位和细胞内ROS水平。结果表明,当冷冻稀释液中添加浓度为40μg/mL辅酶Q10时,经历冷冻—解冻过程的绒山羊精液样本的精子活率、质膜完整率、顶体完整率均显著高于对照组(P<0.05);在冷冻稀释液中添加浓度为40μg/mL或400μg/mL的辅酶Q10均能显著提高线粒体膜电位并降低细胞内ROS水平(P<0.05)。综上所述,在冷冻稀释液中添加40μg/mL的辅酶Q10能够显著提高绒山羊精子抗氧化能力和冷冻保存效果。     

海藻糖、二甲乙酰胺对公猪精液冷冻保存效果的研究

采用5% 二甲乙酰胺(DMA)(V/V)完全替代甘油,比较乳糖、海藻糖对精液冷冻保存效果的影响。结果表明,海藻糖显著提高了冷冻——解冻后精子成活力(49.32%±1.52%)与顶体完整性 (47.33%±1.16%)(P<0.05)。然后利用海藻糖替代乳糖,评价不同浓度的DMA对公猪精液冷冻保存的影响。结果表明,当DMA添加量为4%时,解冻后精子活率、成活力、顶体完整率分别为(45.17±0.56)%、(50.33±0.67)%、(48.30±1.44)%,均显著高于3% DMA、6% DMA添加组(P<0.05),精子活率显著高于5% DMA添加组(P<0.05),但精子成活力、顶体完整性与其差异不显著(P>0.05)。因此,当利用海藻糖作为冷冻保存基础稀释液,DMA最适添加量为4%。     

Cryopreservation of Dog Semen in a Tris Extender with 1% or 2% Soya Bean Lecithin as a Replacement of Egg Yolk

Egg yolk is usually included in extenders used for preservation of dog semen. Lecithin is an interesting animal‐protein free alternative to egg yolk for semen preservation. The aim of our study was to evaluate soya bean lecithin for cryopreservation of dog semen. Five ejaculate replicates were divided in three equal parts, centrifuged and each pellet diluted with one of the three Tris‐based extenders containing 20% egg yolk, 1% soya bean lecithin or 2% soya bean lecithin. Extended semen was loaded in 0.5‐ml straws, cooled and diluted a second time and frozen in liquid nitrogen vapours. Sperm motility parameters (CASA), acrosome integrity (FITC‐PNA/PI) and sperm membrane integrity (C‐FDA) were evaluated 5 min post‐thaw and after 2 and 4 h of incubation. Total motility was significantly better in the egg yolk extender than in any of the lecithin‐based extender and was better in the 1% lecithin extender than in the 2% lecithin extender. Sperm membrane integrity was significantly better in the egg yolk extender than in any of the lecithin‐based extenders but did not differ significantly between the 1% and 2% lecithin extenders. Acrosome integrity was significantly better in the egg yolk extender than in the 2% lecithin extender but did not differ between the egg yolk extender and the 1% lecithin extender or between the two lecithin extenders. In conclusion, egg yolk was superior to lecithin in our study. The extender with 1% lecithin preserved sperm motility better than the extender with 2% lecithin.     

添加低剂量大豆卵磷脂稀释液低温保存绵羊精液的优化研究

本实验旨在探讨添加低剂量大豆卵磷脂稀释液对绵羊精液低温保存效果。实验一采用6个浓度(0%、0.25%、0.5%、0.75%、1.0%、1.25%)大豆卵磷脂替代TRIS专利稀释液中卵黄低温保存绵羊精液,在第0、1、4、7、10、12、18天检测精子活率、顶体完整率;实验二选用实验一中最优添加组(0.5%组)和TRIS专利稀释液制成粉剂低温保存绵羊精液,在保存第0、1、4、7、10、12天对精子活率和顶体完整率进行测定,并在保存第10天对绵羊进行人工授精。结果表明:实验一中,0%组从第1天精子活率低于其他组(P<0.05),0.25%组精子活率观测值从第10天开始低于0.25%以上浓度组(P<0.05),0.5%、0.75%、1.0%、1.25%组保存第10天精子活率均大于50%,0.5%组保存第18天精子活率高于1.0%、1.25%组(P<0.05);各组顶体完整率缓慢下降,各时间点均无显著性差异;实验二中,0.5%组与TRIS组在各时间点的精子活率、顶体完整率与受胎率均无显著性差异,保存第10天精子活率均高于50%;0.5%组受胎率为65.49%,略低于TRIS组67.65%(P>0.05)。本实验条件下,绵羊精液低温保存稀释液中添加0.5%大豆卵磷脂替代卵黄效果最佳。     

Comparison of Different Glycerol and Egg Yolk Concentrations Added to Tris‐based Extender for the Collared Peccaries (Tayassu tajacu) Semen Freezing

This study aimed to evaluate various concentrations of egg yolk (5, 10, or 20%) in combination with different concentrations of glycerol (3% or 6%) added to a Tris‐based extender on the post‐thaw characteristics of sperm obtained from Tayassu tajacu. For this purpose, semen from 10 sexually male mature collared peccaries was collected by electroejaculation and evaluated for sperm motility, vigour, viability, morphology and functional membrane integrity. The ejaculates were initially extended in Tris‐fructose plus egg yolk (5%, 10% or 20%). After cooling, the semen was added to Tris‐egg yolk plus glycerol (6% or 12%), resulting in a final concentration of 3% or 6% glycerol of the extender. Straws were frozen using liquid nitrogen and thawed in a water bath at 37°C for 30 s. The frozen–thawed semen was evaluated as reported for fresh semen. After thawing, a significant decrease was verified for sperm motility and vigour, for all the samples in comparison with fresh semen. However, no differences were evidenced among treatments for any sperm characteristics evaluated (p > 0.05), except for the combination between 10% egg yolk and 6% glycerol, which provided the worst preservation of functional membrane integrity (p < 0.05). The interactions between higher concentrations of egg yolk (20%) and glycerol (6%) and also between lower concentrations of the same substances (5% egg yolk and 3% glycerol) added to the Tris‐based extender negatively affected the preservation of the normal sperm morphology after thawing (p < 0.05). In conclusion, the use of Tris‐based extender added to 10% or 20% egg yolk plus 3% glycerol is recommended for effective sperm cryopreservation in collared peccaries.     

A comparison of duck and chicken egg yolk for cryopreservation of stallion sperm

OBJECTIVE: Duck and chicken egg yolk were compared for their protective effects against cold shock during the cryopreservation of stallion sperm in a lactose-EDTA-glycerol cryodiluent. DESIGN: A completely randomised design was used. Procedure Ejaculates from five stallions (n = 14 ejaculates) were split and diluted to either 20 or 200 x 10(6) sperm/mL in a lactose-EDTA extender containing either duck or chicken egg yolk. The extended semen was then frozen in liquid nitrogen. The percentage of sperm total motility and forward progressive motility were assessed before freezing and at 0 and 1 hr after thawing. Morphology data were also collected at 0 and 1 hr post thaw. RESULTS: Total and forward progressive motility were higher when the sperm were frozen in the presence of duck rather than chicken egg yolk. Furthermore, the total and forward progressive motility and percentage of morphologically normal sperm were higher when frozen at a concentration of 200 than 20 x 10(6)/mL. CONCLUSION: The results of this study demonstrate that the motility parameters of stallion sperm are improved when the semen is frozen in lactose EDTA extender supplemented with duck egg yolk rather than chicken egg yolk. Moreover, sperm motility and the percentage of morphologically normal sperm were higher after freezing at a concentration of 200 x 10(6)/ml rather than 20 x 10(6)/ml.     

Optimization of Ram Semen Cryopreservation Using a Chemically Defined Soybean Lecithin‐Based Extender

The purpose of the present study was to investigate the effects of a chemically defined soybean lecithin‐based semen extender as a substitute for egg yolk‐based extenders in ram semen cryopreservation. In this study, 28 ejaculates were collected from four Zandi rams in the breeding season and then pooled together. The pooled semen was divided into six equal aliquots and diluted with six different extenders: (i) Tris‐based extender (TE) containing 0.5% (w/v) soybean lecithin (SL0.5), (ii) TE containing 1% (w/v) soybean lecithin (SL1), (iii) TE containing 1.5% (w/v) soybean lecithin (SL1.5), (iv) TE containing 2% (w/v) soybean lecithin (SL2), (v) TE containing 2.5% (w/v) soybean lecithin (SL2.5) and (vi) TE containing 20% (v/v) egg yolk (EYT). After thawing, sperm motility and motion parameters, plasma membrane and acrosome integrity, apoptosis status and mitochondrial activity were evaluated. The results shown that total and progressive motility (54.43 ± 1.33% and 25.43 ± 0.96%, respectively) were significantly higher in SL1.5 when compared to other semen extenders. Sperm motion parameters (VAP, VSL, VCL, ALH and STR) were significantly higher in SL1.5 compared to other extender, with the exception of SL1 extender. Plasma membrane integrity (48.86 ± 1.38%) was significantly higher in SL1.5 when compared to other semen extenders. Also, percentage of spermatozoa with intact acrosome in SL1.5 (85.35 ± 2.19%) extender was significantly higher than that in SL0.5, SL2.5 and EYT extenders. The results showed that the proportion of live post‐thawed sperm was significantly increased in SL1.5 extender compared to SL0.5, SL2 and EYT extenders. In addition, SL1, SL1.5 and SL2.5 extenders resulted in significantly lower percentage of early‐apoptotic sperm than that in EYT extender. There were no significant differences in different semen extenders for percentage of post‐thawed necrotic and late‐apoptotic spermatozoa. Also, the results indicated that there are slight differences for percentage of live spermatozoa with active mitochondria between extenders. In conclusion, SL1.5 extender was better than other extenders in most in vitro evaluated sperm parameters.     

不同卵黄成分、大豆卵磷脂及β-环糊精胆固醇对马精子冷冻效果的影响

本研究旨在比较添加不同浓度的卵黄成分（全卵黄（EY）、卵黄浆蛋白（EYP）、低密度脂蛋白（LDL）及水溶性卵黄蛋白（livetin））、大豆卵磷脂（SL）及β-环糊精胆固醇（CLC）对马精子冷冻效果的影响。选择4匹成年英纯血种公马进行采精,试验1：以INRA82+3.5%的混合抗冻剂作为基础冷冻液,添加0、2%、4%和8%浓度的EY、LDL、EYP或水溶性卵黄蛋白;试验2：以4%全卵黄组为对照,添加1%、2%和4% SL;试验3：在INRA82+3.5%的混合抗冻剂+4% EY基础液中添加0、0.75、1.5和3 mg/mL浓度的CLC。结果显示,EYP、LDL及水溶性卵黄蛋白3种卵黄组分均对马精子冷冻具有显著保护作用（P<0.05）,但不同卵黄组分之间及与EY组相比保护效果无显著差异（P>0.05）;添加低浓度（1%）的SL精子冻融后活力与对照组（4% EY组）相比无显著差异（P>0.05）,但添加高浓度SL组（2%和4%）冻融后精子TM、PM及RAP值均显著低于对照组（P<0.05）;添加不同浓度的CLC解冻后精子活力与未添加组无显著差异（P>0.05）。研究结果表明,卵黄中EYP、LDL和水溶性卵黄蛋白均对精子保护有效,保护效果与EY无显著差异;1% SL可获得与卵黄相似的保护效果;在含有卵黄的基础冷冻液中添加CLC未能提高精子冻后活力。     

鸵鸟卵黄对猪精子冷冻后质量的影响

为了提高猪冷冻精液品质和精子抵抗低温打击的能力,本研究以5%、10%、15%、20%和25%等不同浓度的鸵鸟卵黄作为冷冻保护剂,以20%的鸡蛋卵黄和20%的鸽蛋卵黄为对照,将冷冻-解冻后的精子活率、质膜完整率和顶体完整率作为评价指标,分析鸵鸟卵黄对猪精子的抗冷冻保护作用。结果表明：稀释液中添加20%鸽蛋卵黄时,精子活率、顶体完整率和质膜完整性分别为52.11%、55.62%和54.94%,显著高于其他组（P〈0.05）。虽然稀释液中添加15%鸵鸟卵黄时,冷冻-解冻后精子活率、顶体完整率和质膜完整率显著高于5%、10%、20%和25%鸵鸟卵黄组,但仍然显著低于稀释液中添加20%鸽蛋卵黄处理组。本研究表明,鸵鸟卵黄在冷冻过程中对猪精子具有一定的保护作用,但相对于鸽子蛋和鸡蛋卵黄效果并不理想。     

纳米化红景天多糖对猪精液冷冻效果的影响

在冷冻稀释液中分别添加分数为1％、5％、10％、15％、20％的纳米化红景天多糖（Nonomaterialsrhodiolasa—chalinensispolysaccaride,NRSP）溶液,以添加6mg／L红景天多糖（Rhodiolasachalinensispolysaccaride,RSP）为对照组,不添加RSP为空白组,检测其对猪冷冻精子活力、顶体完整率、质膜完整率等生理结构指标,以及精子抗氧化能力的影响。结果表明,添加体积分数为15％NRSP组的精子活力（0．69）、顶体完整率（51．42％）、质膜完整率（25．95％）均高于其他组,并显著高于空白组（P〈0．05）。此外添加15％NRSP组的解冻后直线运动精子百分比（46．33％）要显著高于其他各组（P〈0．05）;添加6mg／LRSP组丙二醛（MDA）浓度最低（10．83±0．39）／2mol／L,并且显著低于空白组（31．85土1．36）μmol／L,（P〈0．05）,但与15％的NRSP组（11．60±0．42）μmol／L无显著差异（P〉0．05）。添加20％的RNSP组超氧化物歧化酶（SOD）活力最高（343．05-＋-19．64）μ／mgprot,显著高于其他添加组（P〈0．05）。精子活力、顶体完整率、质膜完整率之间存在显著的正相关关系（P〈0．05）,而精子活力和顶体完整性与MDA浓度呈显著负相关（P〈0．05）,与SOD活力呈显著正相关（P〈0．05）,质膜完整率与两者含量相关性不显著（P〉0．05）。