大黄鱼生长激素在毕赤酵母中的表达

为大量获得具有生物活性的大黄鱼生长激素(Pseucdosciaena crocea，Growth Hormone，pcGH),利用本实验室分离的天然大黄鱼生长激素基因在毕赤酵母表达系统中的表达进行了研究。把pcGH克隆到酵母整合型质粒载体中构建重组表达载体pPIC9K-pcGH，电击转化将pcGH转化进his4缺陷型毕赤酵母GSll5菌株染色体上，经甲醇诱导得以高效表达，SDS-PAGE证实了表达产物为大黄鱼生长激素，表达量约为62.5 mg/L。

Expression of Pseucdosciaena crocea Growth Hormone in Yeast Pichia Pastoris

This study aimed at high-yield expression of the pcGH gene which was separated from natural large yellow croaker in the yeast Pichia pastoris in order to produce amount of large yellow croaker growth hormone (Pseucdosciaena crocea, Growth Hormone, pcGH) with the feature of biological activity for further use. The pcGH gene was first cloned into the integrative expression vector of methylotrophic yeast P. Pastoris to form the recombinant vector pPIC9K-pcGH, and the linearized recombinant plasmid was then transformed into the chromosome of the HIS4 mutant yeast GS115 by electroporation, and it could finally get high-yield expression induced by methanol. SDS-PAGE confirmed that the expression product was pcGH, and it led to a level as high as of 62.5 mg/L.