Goats are susceptible to Bubaline alphaherpesvirus 1 infection: Results of an experimental study

Herpesvirus infections are generally subjected to strong host species restriction, although virological and serological investigations have revealed the possibility of cross-species infections in closely related animal species. In this study we evaluated susceptibility of goats to infection by Bubaline alphaherpesvirus 1 (BuHV-1). Four goats were inoculated intra-nasally with BuHV-1 and monitored clinically, virologically and serologically for 42 days. None of the goats displayed clinical signs although all the animals variably shed the virus by the nasal route during the first 12 days after infection. BuHV-1 was also detected in the white blood cells of two animals in the first week post infection. The results suggest that goats are susceptible to BuHV-1 infection and that they could play an epidemiological role in the circulation/transmission of the virus among domestic and wild ruminants and impact to some extent on the control plans for herpesviruses in cattle.     

基于模糊评判法的长安区滑坡崩塌灾害易发性分区评价

在对长安区滑坡崩塌地质灾害发育现状及形成条件分析的基础上,结合地质灾害易发性分区评价方法,采用模糊综合评判法对长安区滑坡崩塌地质灾害进行了易发性区划评价。结果表明,长安区滑坡崩塌易发性分区分为6个高易发区域、3个中易发区、1个低易发区(C)和1个不易发区(D),高易发区总面积为190.94 km2,中易发区为382.93 km2,低易发区为299.21 km2,不易发区为707.56km2。     

Characterization of Leptospira infection in suckling and weaning rat pups

Rats are known to be the most important reservoirs of Leptospira spp. However, the leptospiral dose and age at which rats become resistant to Leptospira infection are not yet well elucidated. Aimed to characterize leptospirosis in rat pups, we found that suckling pups (4-, 7-, and 14-day old) are susceptible to leptospires and resistance starts from the weaning age (23-day old). Susceptibility of rat pups was also affected by the infecting dose of the organisms. Jaundice, decrease in body weight, and neurological symptoms prior to moribundity was evident in infected suckling pups. However, 23-day-old infected pups did not manifest any pathological changes and were able to survive the infection similar to adult rats. Based on these results, we propose the suckling rat pup as a novel animal model of human leptospirosis to investigate pathogenesis, development of host resistance, and the mechanisms involved in rats becoming maintenance hosts for leptospires.     

离心式玉米破碎敏感性测试仪设计和性能测试

设计了离心式玉米破碎敏感性测试仪，选择了操作参数，并进行了性能试验。结果显示，离心式破碎敏感性测试仪与Ｗｉｓｃｏｎｓｉｎ破碎测试仪性能相似，比Ｓｔｅｉｎ破碎测试仪性能可靠。对不同湿度的玉米具有较高的敏感性，对不同品种和不同处理条件下的玉米适应性强，变异系数小，再现性好，可迅速和准确测定玉米破碎敏感性     

小麦收获前种子发芽敏感性配合力的研究

选用5个收获前穗发芽敏感性不同的白皮小麦亲本品种,进行双列杂交,研究了发芽敏感性的配合力。结果表明:小麦收获前种子发芽敏感性同时受加性和非加性基因效应影响,而加性基因效应起主导作用;亲本品种发芽敏感性的一般配合力存在极显著差异,杂交组合的特殊配合力和反交效应方差则有时显著,有时不显著;白皮小麦杂交当代种子,以     

大豆品种的再生性能及对EHA 101农杆菌的敏感性

大豆转化可利用农杆菌和子叶节转化系统,bar基因作为选择标记,草丁膦作为选择试剂.用5-6 d发芽的种子的子叶节作外植体,在子叶节处划5-6下,用含pPTN 140的农杆菌EHA 101感染后,共培养3 d,用含抗生素的洗液洗去外植体上的农杆菌,将外植体放入5 mg/L草丁膦的长芽培养基,两周后统计不同大豆品种的再生率,4周后做GUS染色对含pPTN1     

Victoria Blight,defense turned upside down

Genes that confer disease resistance to biotrophic pathogens typically encode nucleotide-binding, leucine-rich-repeat proteins (NB-LRRs). These proteins confer resistance by detecting the presence of virulence effectors secreted by biotrophic pathogens. Recognition triggers NB-LRR activation and subsequently, the defense response which often includes localized host cell death. The fungus, Cochliobolus victoriae, is a necrotrophic pathogen that causes a disease called Victoria Blight. Virulence of this fungus is dependent on its production of a peptide called “victorin” that has been traditionally described as a toxin. Only plants that respond to victorin are susceptible to Cochliobolus victoriae whereas those that do not are resistant to the fungus. Genetic and molecular analyses have revealed that victorin functions like a biotrophic effector recognized by a NB-LRR resistance protein in Arabidopsis. Further, numerous plant species express victorin sensitivity suggesting there are numerous NB-LRRs that recognize victorin. Thus, through expression of victorin, C. victoriae is able to exploit plant defense to cause disease and is capable of evoking this response in an array of different plants.     

Susceptibility by amberjack (Seriola dumerili), yellowtail (S. quinqueradiata) and Japanese flounder (Paralichthys olivaceus) to Neobenedenia girellae (Monogenea) infection and their acquired protection

This study investigated: 1) susceptibility differences to infection by Neobenedenia girellae (Capsalidae) between amberjack Seriola dumerili (Carangidae), yellowtail S. quinqueradiata and Japanese flounder Paralichthys olivaceus (Paralichthyidae); 2) growth and egg production of N. girellae on each fish species; 3) acquired protection of each fish species against this parasite. The number of N. girellae on S. dumerili was significantly higher than on S. quinqueradiata and P. olivaceus when these fishes were exposed to oncomiracidia in the same aquarium. Neobenedenia girellae growth on S. dumerili was fastest and, thus the number of eggs laid by parasites on S. dumerili was greater than on the other two species. Seriola dumerili and P. olivaceus, which were previously infected with N. girellae and treated by freshwater bath, acquired partial protection against re-infection by N. girellae. The relative re-infection of three S. dumerili individuals out of eleven individuals was markedly low compared with the initial infection, and the relative initial infection and re-infection on two P. olivaceus out of eleven individuals was markedly low. The results of this study could be useful to control N. girellae infections when cultivating S. dumerili, S. quinqueradiata and P. olivaceus.     

柚矮化病的品种感病性调查

以特征性症状春梢叶片扭曲畸形和枝条茎陷点为诊断依据，调查结果表明，５５个柚类品种中有２２个发病，占调查品种数的４０％；２８个沙田柚砧穗组合中有２２个发病，占调查砧穗组合数的７８．５％。以沙田柚实生苗和酸柚实生苗作指示植物，对温州蜜柑上的类似矮化病的叶片扭曲症状进行鉴定的结果，指示植物上表现出与接种沙田柚矮化病病原的指示植物相似的春梢叶片扭曲及茎陷点症状。     

甘蓝蚜对杀虫剂的敏感性测定

采用微量点滴法,测定了甘蓝蚜对杀虫剂的敏感性。研究结果表明:不同地形条件菜园甘蓝蚜种群对药剂敏感性存在差异,山区种群对药剂的敏感性高于平原种群,在供试药剂中各种群对齐螨素、吡虫啉和辛硫磷的敏感性表现较高;油菜上的甘蓝蚜种群对药剂的敏感性较高,其中对啶虫脒、吡虫啉、马拉硫磷、齐螨素、辛硫磷、氯氰菊酯存在显著的差异;甘蓝结球期甘蓝蚜种群对药剂敏感性高于甘蓝莲座期,对啶虫脒、吡虫啉、马拉硫磷、齐螨素、辛硫磷、氯氰菊酯存在显著的差异。