益生芽孢杆菌B.licheniformis29质粒Pbl29性质的研究

质粒pBL29为闭合环状质粒，酶切分析证明质粒pBL29有大量的单酶切位点，如EcoR I、Sma I、Hind Ⅲ、Bgt Ⅱ、Pst I、Xba I、BamH I等，并根据限制性酶切各片段的分子量作出了质粒pBL29的内切酶图谱。对质粒pBL29进行测序和分析，证明了其大小为3711bp，具有大量的单酶切位点、编码卡那霉素抗性基因以及富含A／T序列的复制起点。     

食药用真菌翅鳞伞菌丝体蛋白活性的研究

对翅鳞伞菌丝体的胞内粗蛋白进行分离、纯化、筛选,获得了抗水稻纹枯病菌的翅鳞伞纯化菌丝体蛋白PSPB-2。通过荧光显微镜观察PSPB-2对人肺癌细胞株（A549）的影响,发现PSPB-2会引起A549出现典型的细胞凋亡现象。进一步研究发现PSPB-2有限制性核酸内切酶的活性。说明菌丝体蛋白PSPB-2具有抗真菌作用的同时又具有抗肿瘤作用。     

葡萄无核基因SCAR标记的序列构成与酶切分析

利用葡萄无核基因的特异探针１８ｂｐ,通过ＰＣＲ扩增获得了与葡萄无核基因相连锁的ＳＣＡＲ标记约５９０ｂｐ,采用自动荧光ＤＮＡ测序仪对该片段的核苷酸组成进行双向测序,来自无核白的无核基因特异标记由５６９对核苷酸组成。该标记可以作为合成探针和设计特异引物进行ＰＣＲ扩增的基础,用于检测葡萄育种材料和无核品种的无核性。     

鹌鹑腺病毒的分离及病原特性的研究

1994年～ 1995年 ,从云南呈贡县爆发的一群有减蛋综合征症状 ,血清含有EDSV血凝抑制抗体的鹌鹑泄殖腔分离到一株病毒 ,并命名为QAVc 94株。经形态学、培养特性、血凝特性、理化特性、免疫电镜、酶切图谱分析、交叉血凝抑制试验以及动物接种结果确定 ,QAVc 94株属于禽腺病毒Ⅲ群中血清型相同而基因型不同的鹌鹑减蛋综合征病毒。病毒颗粒略似球形 ,无囊膜 ,直径为 70～ 75nm ,核酸大小为 34 7kb ,分子量为 2 2 9× 10 6Da ,其TCID50 为 4 5。     

花椰菜单染色体的显微分离和LA-PCR扩增

 以花椰菜品种‘闽花60天’根尖为材料,采用微细玻璃针法随机分离了40多条花椰菜的单染色体,并用LA-PCR(linker-adaptorPCR)对分离的单染色体进行了体外扩增,研究了单染色体的Sau3A酶切时间对LA-PCR扩增片段大小的影响。结果表明,通过对单染色体的不完全酶切可以显著提高LA-PCR扩增片段的大小;Dot-blotting验证的结果表明,单染色体的扩增产物确实来自其基因组,但Dot-blotting不能有效地鉴别花椰菜的9条染色体。     

Value of endonuclease domain containing 1 in progression of prostate cancer

AIM: To analyze the difference of endonuclease domain containing 1 (ENDOD1) expression between benign prostatic hyperplasia (BPH) tissues and prostate cancer (PCa) tissues and to investigate the effect of ENDOD1 on the biological function of human prostate cancer cells. METHODS: The BPH samples (n=20) and PCa samples (n=21) were processed and analyzed according to the instruction of immunohistochemical (IHC) staining. The mRNA and protein levels of ENDOD1 in the normal prostate epithelial cells and prostate cancer cells were evaluated by RT-qPCR and Western blot, respectively. The recombinant plasmids pCMV-N-Flag-ENDOD1 was constructed and was transfected into the human prostate cancer cells. The proliferation, apoptosis, migration and invasion abilities of the prostate cancer cells were evaluated by MTT assay, flow cytometry, Transwell migration and Matrigel invasion assays, respectively. RESULTS: The analysis of variance of the immunoreactivity score showed that PCa tissues with high Gleason score displayed significantly lower ENDOD1expression than that with low Gleason score and BPH (P<0.05). The expression of ENDOD1 at mRNA and protein levels in PC3 cells and DU145 cells was significantly lower than that in the LNCap cells (P<0.05). The proliferation of DU145 transfected with ENDOD1 was inhibited. The flow cytometry indicated that ENDOD1 over-expression in the DU145 cells resulted in a notable increase in G₀/G₁ phase arrest (P<0.05), but the apoptotic rates showed no statistical difference. The results of Transwell assay showed that migration and invasion abilities of the cells were also inhibited after transfection with over-expressing ENDOD1 plasmid (P<0.05). CONCLUSION: The expression of ENDOD1 significantly decreased in prostate cancer with high Gleaon score. Meanwhile, the ENDOD1 is specifically down-regulated in androgen independent prostate cancer (AIPC) cell lines. Over-expression of ENDOD1 remarkably inhibits the proliferation, migration and invasion abilities of AIPC.