Investigations on the Occurrence of Hereditary Diseases in the Danish Cattle Population 1989-1991

Agerholm, J.S., A. Basse and K. Christensen: Investigations on the occurrence of hereditary diseases in the Danish cattle population 1989-1991. Acta vet. scand. 1993, 34, 245-253.– The methods of the Danish Bovine Genetic Disease Programme are outlined, and the results obtained during the first 3 years in function are described. The most common disease reported was spinal muscular atrophy in calves of the Red Danish Dairy breed with 312 reports. Necropsy was performed on 162 cases, and spinal muscular atrophy was diagnosed in 82 of these. Bovine progressive degenerative mye-loencephalopathy, rectovaginal constriction, syndrome of arthrogryposis and palatoschisis, hereditary chondrodysplasia (2 different types), syndactylism, epitheliogenesis imperfecta, and osteogenesis imperfecta was diagnosed with 1 case each. Lethal trait A46 was diagnosed in 4 calves. Some of these diseases have not previously been described in Denmark, and epitheliogenesis imperfecta was for the first time diagnosed in the Hereford breed. Chromosome translocation 1/29 was detected in the Blonde d’Aquitaine (BAQ), Limousine, and Red Danish Dairy breed. The aberration occurred frequently in BAQ. Furthermore, a complex chromosome translocation t(l;8;9)(q45;ql3;q26) was detected in the Red Danish Dairy breed.     

猪肺炎支原体膜蛋白P46基因在大肠杆菌中的表达

把猪肺炎支原体(Mycoplasma hyopneumoniae)国际标准株232的P46基因克隆进pGEM-T-EASY载体上,通过PCR方法把该基因中三个编码Trp的密码子TGA突变为TGG,然后将该基因亚克隆到载体pMAL-P2X上,得到重组表达载体pMAL-P2X-P46.用该重组载体转化大肠杆菌TB1,得到表达重组菌TB1-pMAL-P2XA-P46,用终浓度为0.3 mM的IPTG在37℃下诱导表达,获得可溶性表达的融合蛋白MBP-P46,在免疫印迹试验中,兔抗MBP高免血清和兔抗猪肺炎支原体高免血清都能与目的蛋白发生阳性反应,证明猪肺炎支原体P46基因在大肠杆菌里获得了可溶性表达.该融合蛋白对于建立特异性和敏感性好的EIISSA方法具有重要意义.     

锥—46抑制[^3H]次黄嘌呤掺入伊氏锥虫核酸的研究

用液体闪烁计数法对锥—46的抗锥虫作用的机理做了初步研究。发现T-46与Berenil很相似,均可显著抑制[~3H]次黄嘌呤掺入伊氏锥虫,抑制作用与浓度及时间呈正相关。T-46和Berenil对DNA合成的IC_(60)分别为1.33和1.73μg·ml~(-1)。本实验还提示T-46抗锥虫作用可能与损伤DNA模板有关。     

秋冬季节柿属植物树体内酚类物质含量的变化

本测试结果表明 ,原产日本中部的栽培柿‘富有’(Diospyros kaki cv.Fuyou)的抗寒性为 (- 2 5±0 .7)℃ ,而原产于亚热带台湾的台东豆柿 (D.taitoensis)的抗寒性仅为 (- 18± 0 .6 )℃。试材枝内酚类物质总含量在 9～ 11月中旬上升至最大值 ,富有柿增加了 0 .7倍 ,台东豆柿增加了 0 .8倍。此后至 2月中旬维持在较高水平 ,3月中旬以后迅速下降。 9～ 11月中旬富有柿枝内黄酮类物质含量显著上升了近 4倍 ,台东豆柿增加了近 1倍 ;此后至 2月中旬维持在较高水平 ,3月中旬以后迅速下降。 2树种枝条皮层中的酚类物质总含量分别为其木质部的 5倍左右。酚类物质的大部分为缩合型单宁 ,其含量变化曲线近似于酚类物质总含量的曲线。试材枝内简单酚类物质和可溶性单宁含量极低 ,且在秋冬季节无显著变化。与富有柿相比 ,台东豆柿枝内酚类物质中简单酚类物质比例高 ,而缩合单宁含量所占比例较低。结果显示 ,柿属植物树体内酚类物质组成及含量与其抗寒力的获得有一定关系     

四川冕宁县缺硒饲粮对雏鸡的影响及不同品种雏鸡适宜补硒量的研究

采用四川严重缺只硒地区冕宁县实用雏鸡饲粮饲喂泸宁鸡(当地地方鸡)、白洛克和来杭三个品种共682只雏鸡,作了两个试验,结果表明:谷实类缺硒饲粮(含硒0.0051ppm)引起雏鸡严重营养性胰腺萎缩和纤维化;泸宁鸡和白洛克缺硒死亡率分别为83％和63％,添加维生素E可起到轻微缓解作用(缺硒死亡率52％);补硒显著促进生长和改善饲料利用率。饲粮中添加动物性饲料(使基础日粮含硒0.1018ppm)后雏鸡未出现明显缺硒病变,但补硒显著提高谷胱甘肽过氧化物酶(GSH-px活力、组织硒含量和生长速度。雏鸡对缺硒的敏感程度、组织硒含量和GSH-px活力存在明显品种差异。以GSH-px活力和组织硒含量作为评定标准结合对生长速度的考察表明:对于生长较快的泸宁鸡和白洛克,基础饲粮补加0.20ppm硒是适宜的,而对于蛋用型来杭鸡则以添加0.10ppm效果较好。     

优质高产杂交中籼新组合辐优155的选育与应用研究

[目的]为杂交中籼新组合辐优155的推广应用提供参考.[方法]通过辐优155经安徽省2007、2008年2年区试和2009年生试,比较辐优155与对照品种Ⅱ优838主要农艺性状、产量、稻米品质及抗性,并分析了其大田栽培技术及制种技术要点,以探讨其生产潜力及推广价值.[结果]辐优155是安徽省铜陵县农业科科学研究所用辐88A与恢155配组育成的杂交中籼新组合,具有株型适中、穗大粒多、米质优、抗稻瘟病、中抗白叶枯病等特点,2010年通过安徽省农作物品种审定委员会审定.[结论]辐优155产量高,品质好,适宜在安徽全省种植.     

人CD46基因启动子的克隆和启动特性研究

为研究人CD46(hCD46)基因启动子的启动特性,研制具有hCD46组织特异性表达特征的转基因小鼠,设计扩增hCD46基因启动子的PCR引物,用HeLa细胞基因组DNA为模板扩增hCD46基因启动子DNA片段,测序结果表明:其序列与GenBank中hCD46完整基因5′端某片段的同源性高达99.9%。用此DNA片段替换表达载体pcD-NA3EGFP中的CMV启动子,且在该片段与EGFP基因之间插入兔β-球蛋白基因的第2内含子RGI。重构的表达载体在2种鼠源细胞CHO和SP2/0中均可启动EGFP表达,表达量与人体内同源组织中hCD46的相似,说明该研究克隆了结构和功能正确的hCD46基因启动子。     

Effects of baicalin on CA46 cell xenografts in nude mice

AIM: To study the effects of baicalin on CA46 cell xenografts in nude mice. METHODS: The nude mice with CA46 cell xenografts were treated with drugs via intraperitoneal injection daily, and were divided into 5 groups: negative control group, 15 mg/kg baicalin group, 30 mg/kg baicalin group, 60 mg/kg baicalin group and 4 mg/kg etoposide (VP-16) positive control group. After 12-day treatment, the weight of CA46 cell xenografts stripped from some nude mice in the 5 groups was used to evaluate the effect of baicalin on xenograft growth in the nude mice. The apoptosis, necrosis and pathological changes of the xenograft cells were examined under light microscope and transmission electronic microscope respectively. The expression levels of phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt) signaling pathway-related proteins extracted from xenografts were determined by Western blotting. The other nude mice with CA46 cell xenografts in the 5 groups continued to be treated with the drugs until death in order to evaluate the effect of balcalin on survival time of the nude mice with CA46 cell xenografts. RESULTS: Baicalin remarkably inhibited the growth of CA46 cell xenografts, induced apoptosis and necrosis of xenograft cells, and reduced the protein expression of phospho-Akt (p-Akt), nuclear factor-kappa B (NF-κB), mammalian target of rapamycin (mTOR) and phospho-mTOR (p-mTOR) in the xenografts after 12-day treatment. Furthermore, baicalin prolonged the survival time of the nude mice with CA46 cell xenografts in a dose-dependent manner. CONCLUSION: Baicalin inhibits the growth and induces apoptosis of CA46 cell xenografts in the nude mice, and prolongs the survival time of the nude mice with CA46 cell xenografts through the mechanism of down-regulating PI3K/Akt/NF-κB and PI3K/Akt/ mTOR signaling pathways.     

氮肥不同施入量对水稻新品种富友33生育及产量的影响

采用小区对比试验方法,探讨了氮肥不同施入量对水稻新品种富友33生育及产量的影响。结果表明,增加氮肥施入量,可促进富友33单位面积收获穗数、齐穗期叶面积指数与剑叶净光合速率、成熟期干物质积累量的增加;不利于其成穗率、齐穗期高效叶面积率与有效叶面积率、齐穗后干物质积累量占籽粒产量百分比以及收获指数的提高。在本试验条件下,施氮量(N)240.0 kg/hm2的处理E3获得单产9.22 t/hm2,比E4、E2、E5、E1处理分别增产了0.2%、3.1%、3.4%和7.3%。     

Synthetic tetramer of a Phytophthora sojae-inducible fragment from soybean GmaSKTI36 promoter improves its pathogen induction activities

Using pathogen-induced promoters to control expression of the functional genes in transgenic plants may greatly increase the chances of boosting disease resistance. However, the number of the inducible promoters is limited. Here, we found that soybean GmaSKTI36 gene is strongly induced upon Phytophthora sojae infection. Functional analysis showed that its promoter could mediate rapid and strong induction of GUS expression upon pathogen infection in both Nicotiana benthamiana leaves and soybean hairy roots. Then, a 122 bp fragment that was critical to the activity was successfully identified by a progressive 5′ deletion analysis. Importantly, we found that a synthetic promoter by tetramerizing this fragment could confer strong P. sojae induction activities. Overall, the results suggested that the GmaSKTI36 promoter, the 122 bp fragment, and the synthetic promoter are potentially useful pathogen-inducible promoters.