We obtained two lines of Chinese head cabbage (Brassica rapa L. ssp. pekinensis) selfed progenies containing both an anti-sense gene of BcpLH and a gene for resistance to kanamycin by micro-injecting buds of their primary transformants (T0) with Agrobacterium tumefaciens strain LBA4404. 31 positive plants resistant to kanamycien were recovered. Southern blot analysis confirmed the presence of T-DNA in two transgenic plants. One (DHZ-13-1) exhibits the characteristics of out-toward rosette and cauline leaves, and nested flower model in which secondary complete flower developed from the base of the primary ovary and the third flower from the ovary in the secondary flower, and so on, while another(DHZ-6-1) has no phenotype change. ABA and IAA affected the root growth of progeny of DHZ-13-1, but 6-BA was insensitive to hypocotyl growth during its seedling development. 相似文献
This study assessed the effects of stocking density (D02, 0.2 individuals/m2; D03. 0.3 individuals/m2; D04, 0.4 individuals/m2; D05, 0.5 individuals/m2; D06, 0.6 individuals/m2) on Chinese mitten crab (Eriocheir sinensis; 6.25?±?0.11 g) growth performance, tissue indices, antioxidant enzyme activity (total superoxide, catalase, glutathione peroxidase, total antioxidant capacity, and malondialdehyde), and mRNA expression of antioxidant-related genes (SOD, CAT, GPx) in rice crab culture systems. Our results demonstrated that increasing stocking densities decreased the final weight (FBW), weight gain rate (WGR), specific growth rate (SGR), feed conversion efficiency (FCR), and protein efficiency ratio (PER) of the crabs. The hepatosomatic index (HSI) of female crabs in D04 group was higher than that of other groups. The gonadosomatic index (GSI) of the male crabs in the D04 group was significantly higher than that of the other groups(P?<?0.05). The meat yield (MY), total edible yield (TEY), and condition factor (CF) of the female and male crabs in the D04 group were higher than those of the other groups. The antioxidant enzyme activity (T-SOD, CAT, GSH-Px, and T-AOC) of male and female crabs in the D04 group was significantly higher than that of the other groups, and the MDA content of the D04 group was significantly lower than that of the other groups (P?<?0.05). The stocking density of 0.4 individuals/m2 effectively promoted the efficient mRNA expression of SOD, CAT, and GPx genes in the hepatopancreas of male and female crabs. In summary, the optimal stocking density of E. sinensis in the rice crab culture system is 0.4 individuals/m2.