全文获取类型
收费全文 | 89026篇 |
免费 | 5582篇 |
国内免费 | 40篇 |
专业分类
林业 | 3732篇 |
农学 | 3462篇 |
基础科学 | 632篇 |
10915篇 | |
综合类 | 13433篇 |
农作物 | 3288篇 |
水产渔业 | 4910篇 |
畜牧兽医 | 47557篇 |
园艺 | 1161篇 |
植物保护 | 5558篇 |
出版年
2020年 | 732篇 |
2019年 | 949篇 |
2018年 | 1581篇 |
2017年 | 1845篇 |
2016年 | 1661篇 |
2015年 | 1406篇 |
2014年 | 1718篇 |
2013年 | 3717篇 |
2012年 | 2929篇 |
2011年 | 3587篇 |
2010年 | 2339篇 |
2009年 | 2329篇 |
2008年 | 3470篇 |
2007年 | 3208篇 |
2006年 | 3135篇 |
2005年 | 2668篇 |
2004年 | 2575篇 |
2003年 | 2549篇 |
2002年 | 2325篇 |
2001年 | 3340篇 |
2000年 | 3249篇 |
1999年 | 2485篇 |
1998年 | 942篇 |
1997年 | 994篇 |
1996年 | 861篇 |
1995年 | 1018篇 |
1994年 | 889篇 |
1993年 | 893篇 |
1992年 | 1844篇 |
1991年 | 1919篇 |
1990年 | 1852篇 |
1989年 | 1855篇 |
1988年 | 1607篇 |
1987年 | 1594篇 |
1986年 | 1627篇 |
1985年 | 1526篇 |
1984年 | 1225篇 |
1983年 | 1032篇 |
1982年 | 713篇 |
1979年 | 1060篇 |
1978年 | 816篇 |
1975年 | 815篇 |
1974年 | 897篇 |
1973年 | 864篇 |
1972年 | 805篇 |
1971年 | 795篇 |
1970年 | 800篇 |
1969年 | 780篇 |
1968年 | 708篇 |
1967年 | 734篇 |
排序方式: 共有10000条查询结果,搜索用时 203 毫秒
1.
A. Susan M. K. Yadav S. Kar S. Aravindan U. Ngangkham S. Raghu S. R. Prabhukarthikeyan U. Keerthana S. C. Mukherjee J. L. Salam T. Adak A. Banerjee P. C. Rath 《Plant pathology》2019,68(3):537-546
Rice blast disease caused by the fungus Magnaporthe oryzae is one of the most devastating diseases causing huge losses worldwide. In the present study, major blast resistance genes were investigated in landraces originating from northeastern India. Based on phenotypic evaluation, 288 landraces were classified into three distinct groups: resistant (75), moderately resistant (127) and susceptible (86). The genetic frequencies of the 18 major blast resistance genes were between 6.2% and 27.4%, with only two genotypes possessing a maximum of nine blast resistance genes. The cluster and population structure analysis grouped the landraces into two groups. Through principal coordinate analysis, the scatter plots partitioned the resistant and moderately resistant landraces into different groups. Analysis of molecular variance showed maximum (96%) diversity within populations and least (4%) diversity between populations. Association analysis identified six markers, CRG4_2, RM72, tk59-2, pi21_79-3, RM1233 and RM6648, that are significantly associated with blast disease and explained a phenotypic variance of 1.1–6.5%. The associated genes could be used in marker-assisted rice breeding programmes for gene pyramiding to develop rice varietal resistance against blast disease. The present study represents a valuable blast resistance genetic resource that could be used for identification of new R genes, donors for blast resistance, and genomic studies. 相似文献
2.
Plant eradication is difficult, particularly in remote, protected areas. The Southern Ocean Islands are very isolated and highly protected, but the flora contains many alien plants. Small restricted populations have been eradicated, but eradication of established species has proven difficult. A better understanding of the efficacy of control methods at sub‐Antarctic temperatures and their off‐target impacts may increase eradication success. With interest in controlling non‐native Poa annua in the region, we aimed to determine if physical and chemical methods can control P. annua (the sub‐Antarctic biotype) in sub‐Antarctic conditions and examined their impact on native plants. We quantified the effectiveness of physical control methods on P. annua in situ on sub‐Antarctic Macquarie Island through field‐based experiments and assessed their selectivity on P. annua compared with native grasses. We also quantified the effectiveness of several herbicides on P. annua at sub‐Antarctic temperatures and assessed their selectivity on native grasses. Of the four physical disturbance methods tested, none effectively reduced P. annua cover as one‐off treatments. Of the herbicide treatments, glyphosate and trifloxysulfuron sodium were effective and were less damaging to native grass species, indicating potential selectivity. Physical control was of limited effectiveness, but did not affect native species richness. An integrated weed management programme utilising the strategic use of selective herbicides with follow‐up chemical and physical intervention may balance control and biodiversity outcomes. This research highlights the importance of site‐specific testing of control methods and understanding off‐target impacts of control when managing alien plant species in protected areas. 相似文献
3.
S. Skov Hansen C. Mattei H. Treffenberg Pettersson M. Grabski 《Equine Veterinary Education》2021,33(11):e433-e437
This report describes successful surgical correction and long-term survival of a case of large colon atresia in a 24-h-old Warmblood colt, referred with signs of unrelenting abdominal pain and a suspicion of meconium impaction. Radiographic and ultrasonographic examination was indicative of large colon meconium impaction with secondary ileus. Due to deterioration of the foal, surgery was recommended. An atretic segment was found between the left ventral and dorsal large (ascending) colon. A band of fibrous tissue with no identifiable lumen connected the segments. Surgical correction was done by performing a stapled side-to-side anastomosis. Twelve days after surgery, the foal was discharged. Twenty-two months after discharge, the owner reported the foal developing as expected compared with its peers, but had a mild, self-limiting episode of colic at 20 months of age. 相似文献
4.
M. A. Macedo A. K. Inoue-Nagata T. N. Z. Silva D. M. S. Freitas J. A. M. Rezende J. C. Barbosa M. Michereff-Filho A. R. Nascimento A. L. Lourenção A. Bergamin Filho 《Plant pathology》2019,68(1):72-84
Efficient management of whitefly-borne diseases remains a challenge due to the lack of a comprehensive understanding of their epidemiology, particularly of the diseases tomato golden mosaic and tomato yellowing. Here, by monitoring 16 plots in four commercial fields, the temporal and spatial distribution of these two diseases were studied in tomato fields in Brazil. In the experimental plots these diseases were caused by tomato severe rugose virus (ToSRV) and tomato chlorosis virus (ToCV), respectively. The incidence of each virus was similar in the plots within a field but varied greatly among fields. Plants with symptoms for both diseases were randomly distributed in three of four spatial analyses. The curves representing the progress of both diseases were similar and contained small fluctuations, indicating that the spread of both viruses was similar under field conditions. In transmission experiments of ToSRV and ToCV by Bemisia tabaci MEAM1 (former biotype B), these viruses had a similar transmission rate in single or mixed infections. It was then shown that primary and secondary spread of ToCV were not efficiently controlled by insecticide applications. Finally, in a typical monomolecular model of disease progress, simulation of the primary dissemination of ToSRV and ToCV showed that infected plants were predominantly randomly distributed. It is concluded that, although the manner of vector transmission differs between ToSRV (persistent) and ToCV (semipersistent), the main dispersal mechanisms are most probably similar for these two diseases: primary spread is the predominant mechanism, and epidemics of these diseases have been caused by several influxes of viruliferous whiteflies. 相似文献
5.
T. Schöneberg K. Kibler F. E. Wettstein T. D. Bucheli H. R. Forrer T. Musa F. Mascher M. Bertossa B. Keller S. Vogelgsang 《Plant pathology》2019,68(1):173-184
High occurrence of Fusarium poae (FP) and Fusarium langsethiae (FL) and their mycotoxins nivalenol (NIV) and T-2/HT-2 have been observed in Swiss oats. Early prediction of mycotoxin levels is important for farmers and the cereal industry to minimize the risk of contaminated food and feed. Therefore, climate chamber experiments were conducted to investigate the influence of different temperatures (10, 15, 20 °C) and durations (4, 8, 12 h) at 99% relative humidity (RH) on the infection of oats with FP and FL. In addition, to discover the most susceptible period of oats, artificial FL inoculations were conducted at different growth stages. Field experiments were performed to observe the dispersal of these fungal species within the field and to investigate the weather conditions that influence the dispersal. The climate chamber experiments revealed higher contamination with NIV and T-2/HT-2 in the 10 °C treatments and with a prolonged humidity duration of 12 h 99% RH. Inoculations of oat plants at early (DC 61) and mid (DC 65) anthesis, led to higher FL infection and T-2/HT-2 accumulation in the grains compared with treatments at earlier growth stages, which might be due to an increased susceptibility during anthesis. No indication for spore dispersal was observed in the field experiments. The results obtained, together with the cropping factors that influence infection and mycotoxin production, could be used as a first step in developing forecasting models to predict the contamination of oats with the mycotoxins NIV and T-2/HT-2. 相似文献
6.
Fruit fly species (Diptera: Tephritidae) of the genus Bactrocera are among the most serious orchard pests worldwide but are not native to Austria. The unexpected finding of one Bactrocera zonata adult in Vienna in 2011 initiated increasing survey efforts in Austria since 2012. Traps with male attracting lures were employed at several sites during the fruiting periods and catches were analysed. At sites in Vienna (urban area) Bactrocera specimens were trapped between 2012 and 2018, whereas no Bactrocera specimens were caught in agricultural areas or commercial orchards outside the city. Twelve specimens were identified as B. zonata and nine specimens as B. dorsalis sensu lato, though for the latter it was not possible to distinguish between B. dorsalis s.l. and B. carambolae using molecular analysis (ITS1). Molecular sequencing showed that the specimens caught were genetically different (with only a few exceptions), indicating genetically different origins. It is evident that the repeated findings of Bactrocera specimens in Vienna, where winter temperatures do not allow the establishment of tropical fruit flies, are linked to repeated entries of juveniles with infested fruits (in luggage or consignments). The results of our study reveal the need to strengthen phytosanitary import requirements for fruit commodities and travellers’ luggage in order to protect fruit production in Austria and probably in other countries. 相似文献
7.
H. A. Narouei-Khandan S. P. Worner S. L. H. Viljanen A. H. C. van Bruggen E. E. Jones 《Plant pathology》2020,69(1):17-27
Myrtle rust (caused by Austropuccinia psidii) affects more than 500 known host species in the Myrtaceae family. Three different modelling approaches (CLIMEX, MaxEnt and Multi-Model Framework) were used to project the habitat suitability for myrtle rust at both global and local scales. Current data on the global occurrence of myrtle rust were collected from online literature and expert solicitation. Long-term averages of climate data (1960–1990) were sourced from WorldClim and CliMond websites. Recent reports of myrtle rust in New Zealand were used for validation of model outputs but not in model training and testing. The model outputs were combined into a consensus model to identify localities projected to be suitable for myrtle rust according to two or three models (hotspots). In addition to the locations where the pathogen is currently present, all models successfully projected independent occurrence data in New Zealand suitable for establishment of the pathogen. Climate suitability for the pathogen was primarily related to temperature followed by rainfall in MaxEnt and the CLIMEX model. The results confirmed the optimum temperature range of this pathogen in the literature (15–25 °C). Additional analysis of the precipitation variables indicated that excessive rain (more than 2000 mm in warmest quarter of the year) combined with high temperatures (>30 °C) constrain pathogen establishment. The results of the current study can be useful for countries such as New Zealand, China, South Africa and Singapore where the pathogen has not fully spread or established. 相似文献
8.
James W. Wynne Chris Stratford Joel Slinger Francisca Samsing Megan Rigby Russell McCulloch Petra Quezada-Rodriguez Richard S. Taylor 《Journal of fish diseases》2020,43(1):39-48
Hydrogen peroxide (H2O2) is a commonly used treatment for a range of parasitic diseases of marine finfish, including amoebic gill disease (AGD). While this treatment is partially effective at reducing parasite load, H2O2 can have detrimental effects on the host under certain conditions. Treatment temperature and dose concentration are two factors that are known to influence the toxicity of H2O2; however, their impact on the outcome of AGD treatment remains unclear. Here, we investigated the effects of treatment temperature (8, 12 or 16°C) and dose concentration (750, 1,000, 1,250 mg/L) on the efficacy of H2O2 to treat AGD. We demonstrated that a 20-min bath treatment of H2O2 at all doses reduced both parasite load and gross gill score significantly. Parasite load and gross gill score were lowest in the 1,000 mg/L treatment performed at 12°C. At the high dose and temperature combinations, H2O2 caused moderate gill damage and a significant increase in the plasma concentration of electrolytes (sodium, chloride and potassium). Taken together, our study demonstrates that higher H2O2 treatment temperatures can adversely affect the host and do not improve the effectiveness of the treatment. 相似文献
9.
10.
拖拉机动量飞轮主动防侧翻控制与模型试验研究 总被引:1,自引:0,他引:1
针对拖拉机侧翻致死致伤事故仍时有发生的问题,基于旋转刚体加速时的反向施矩原理,以反作用动量飞轮为执行元件,提出了从主动安全角度解决拖拉机侧翻问题的研究方法。通过构建拖拉机动力学系统数学模型,解析了整机侧翻行为的动态演变机理。为保证拖拉机主体结构的完整性,将动量飞轮置于拖拉机前部,取代传统静态配重的同时可主动提供防侧翻力矩。应用Matlab/Simulink软件,对反作用飞轮的回稳过程进行了基于PID控制的有效性仿真分析,同时设计并搭建了比例模型试验平台,对主动施矩飞轮的回稳控制效果进行了试验验证。结果表明,装备飞轮的拖拉机与无控制组对比,在一次试验中可多次实现整机的防侧翻控制,使整机防侧翻性能得到明显改善,且不同行驶工况下的试验数据与仿真结果的相关性较强,充分验证了本文拖拉机侧翻动力学模型的有效性。 相似文献