排序方式: 共有36条查询结果,搜索用时 31 毫秒
1.
Mattana Efisio Peguero Brígido Di Sacco Alice Agramonte Wilvin Encarnación Castillo Wilkin Rafael Jiménez Francisco Clase Teodoro Pritchard Hugh W. Gómez-Barreiro Pablo Castillo-Lorenzo Elena Terrero Encarnación Marianny Way Michael J. García Ricardo Ulian Tiziana 《New Forests》2020,51(4):705-721
New Forests - Native trees from the Caribbean were tested for seed desiccation responses, by adapting the “100-seed test” protocol. Ninety-seven seed lots of 91 species were collected... 相似文献
2.
Raquel Sánchez-Pérez Encarnación Ortega Henri Duval Pedro Martínez-Gómez Federico Dicenta 《Euphytica》2007,155(3):381-391
The inheritance of 16 important agronomic traits and its relationship were studied for four years in a population of 167 almond
[P. dulcis (Mill.) D.A. Webb] seedlings obtained from a cross between the French selection ‘R1000’ (‘Tardy Nonpareil’ × ‘Tuono’) and
the Spanish cultivar ‘Desmayo Largueta’. For some traits (blooming and leafing date) descendants segregated between the value
of both progenitors, meanwhile for others the mean of the offspring was lower (bloom density, productivity and ripening date)
or higher (in-shell/kernel ratio and double kernels). As expected, kernel bitterness and self-incompatibility behaved as monogenic
traits. Some important correlations between traits were detected. The implications of the transmission and the correlation
of these traits in the breeding programmes are discussed. 相似文献
3.
Goicoechea E Van Twillert K Duits M Brandon ED Kootstra PR Blokland MH Guillén MD 《Journal of agricultural and food chemistry》2008,56(18):8475-8483
Mixtures of either sunflower oil or thermodegraded sunflower oil and a standard meal were submitted to an in vitro digestion model. The same experiment was carried out with fluid deep-frying fat and thermodegraded fluid deep-frying fat. The thermodegradation of the oil and fat was provoked by submitting them to 190 degrees C with aeration in a convection oven, and the presence in the headspace of the thermodegraded oil and fat of oxygenated alpha,beta-unsaturated aldehydes (OalphabetaUAs), such as 4-hydroxy-2-nonenal (HNE), 4-oxo-2-nonenal (ONE), and 4,5-epoxy-2-decenal (EDE), was monitored by solid phase microextraction (SPME) followed by gas chromatography-mass spectrometry (GC-MS). The digestion products were separated by centrifugation in a lipidic phase, an aqueous phase, and a pellet phase. The headspace of these three phases was also studied by SPME/GC-MS to check if the toxic and very reactive OalphabetaUAs above-mentioned remained unaltered after the in vitro digestion process or if they had reacted with the various compounds present in the digestion products, so disappearing from the samples. With the same aim the extract in ethyl acetate of the aqueous and pellet phases, and of the lipidic phase after dilution, were studied by GC-MS. All results obtained showed that a certain proportion of the toxic OalphabetaUAs remains unaltered after digestion, dispersed in the three phases above-mentioned, and thus are bioaccessible in the gastrointestinal tract and so could reach the systemic circulation. Compounds that may originate in Maillard type reactions (2-pentylpyridine) are found among digestion products, proving that these reactions are possible in this process if adequate substrates are present. In addition, it has been shown that toxic metabolites from the synthetic antioxidant BHT, present in fat before digestion, remain unaltered after this process and could reach the systemic circulation. 相似文献
4.
Iglesias MT Martín-Alvarez PJ Polo MC de Lorenzo C Gonzalez M Pueyo E 《Journal of agricultural and food chemistry》2006,54(24):9099-9104
This study was carried out to establish the changes in the free amino acid contents of floral honeys, honeydew honeys, and blend honeys during storage at room temperature and to test the capacity of the amino acids to distinguish the origin of the honeys after storage. For this purpose, 54 artisanal honeys (39 floral, 5 honeydew, and 10 blend) were studied. Samples were taken from recently collected honeys and at 3, 6, 9, 12, 16, 20, and 24 months after harvesting. The contents of most of the free amino acids were found to decrease with storage time, with the greatest reduction observed in the first 9 months. The contents of the amino acids aspartic acid, beta-alanine, and proline increased in the first few months after storage, reaching maximum values at 6 months, suggesting the possible existence of enzymatic activities. The application of stepwise discriminant analysis to the free amino acid content data demonstrated that the contents of the amino acids valine, beta-alanine, gamma-aminobutyric acid, serine, isoleucine, alpha-alanine, ornithine, and glutamine correctly assigned 87% of honeys to their group of origin: floral, honeydew, or blend. 相似文献
5.
Julio Martin-Mata Raúl Moral Angel Torres-Climent Encarnación Martínez-Sabater Concepción Paredes 《Communications in Soil Science and Plant Analysis》2015,46(4):188-196
The aim of the present article is to show the possibilities of chemometric tools and the parallel factor analysis (PARAFAC) model, as well as to understand the complexities of the fluorescence emission-excitation matrix (EEM) of water-soluble organic matter (WSOM) extracted from sewage sludge samples obtained with different origins and stabilization procedures. The variation in the composition of WSOM in the different sewage sludge samples could be correlated with the conditions of stabilization; therefore, the use of fluorescence spectroscopy to monitor these changes can be a useful tool to optimize the management of this residual flux, avoiding environmental pollution. However, the “contour density” EEM maps obtained with fluorescence present continuous spectra with different peaks, valleys, and shoulders where manual peak picking can be complicated and subjective, especially in complex matrixes such as sewage sludge. With the PARAFAC algorithm, it is possible to resolve the emission and excitation spectra of the different fluorescence components present in the samples and their contribution to the total fluorescence. Also, the contribution (scores) of the different fluorophors can be coupled with another chemometric tool to provide an effective classification method using the stabilization conditions of sewage sludge as main criterion. In this study, a wide survey of sewage sludge samples (287) was characterized by fluorescence emission-excitation matrix. The final molecular composition of the sewage sludge samples was not related to the stabilization (aerobic vs anaerobic) treatments. However, a sewage sludge classification based on a combined spectroscopic-chemometric approach was obtained, which could be used for the optimization of sewage sludge use in agriculture, minimizing the environmental risks. 相似文献
6.
Detection of the Defoliating Pathotype of Verticillium dahliae in Infected Olive Plants by Nested PCR 总被引:2,自引:0,他引:2
Jesús Mercado-Blanco Dolores Rodríguez-Jurado Encarnación Pérez-Artés Rafael M. Jiménez-Díaz 《European journal of plant pathology / European Foundation for Plant Pathology》2002,108(1):1-13
Spread of Verticillium wilt into newly established olive orchards in Andalucía, southern Spain, has caused concern in the olive industry in the region. This spread may result from use of Verticillium dahliae-infected planting material, which can extend distribution of the highly virulent, defoliating (D) pathotype of V. dahliae to new areas. In this study, a molecular diagnostic method for the early in planta detection of D V. dahliae was developed, aimed especially at nursery-produced olive plants. For this purpose, new primers for nested PCR were designed by sequencing a 992-bp RAPD marker of the D pathotype. The use of the specific primers and different nested-PCR protocols allowed the detection of V. dahliae pathotype D DNA in infected root and stem tissues of young olive plants. Detection of the pathogen was effective from the very earliest moments following inoculation of olive plants with a V. dahliae pathotype D conidia suspension as well as in inoculated, though symptomless, plants. 相似文献
7.
María Inés Santín-Montanyá Encarnación Zambrana-Quesada José Luis Tenorio-Pasamón 《Archives of Agronomy and Soil Science》2018,64(12):1705-1713
Weed abundance in crops undergoes frequent changes, often due to changes in tillage practices. Annual species, with quick germination, a short vegetative stage, profuse seed production and long-lived seeds become problematic under zero-tillage systems. Portulaca oleracea L. and Amaranthus blitoides L. are widespread weeds in the Mediterranean area, prominent in irrigated crops. We studied the total weed abundance in the field, and specifically these two species (Portulaca oleracea and Amaranthus blitoides) with high frequency of occurrence in monoculture maize, from 2012 to 2014, in the field and soil seedbank. Results showed significant differences between zero-tillage (ZT) and conventional tillage (CT) systems on total weed abundance and relative abundance of Portulaca oleracea. Total weed abundance decreased in ZT plots (from 136 plants m?2 to 25 and 46 plants m?2, in 2013 and 2014 respectively). The same trend was observed in Portulaca oleracea recorded in ZT plots, but the abundance of Amaranthus blitoides did not vary in this system. Weed seedling germination and weed seed numbers both of total weed seedbank and Portulaca oleracea, were greater in ZT plots compared to CT, regarding Amaranthus blitoides seedling germination and seed count, the values did not increase with ZT, in continuous maize crops. 相似文献
8.
Granado-Lorencio F Donoso-Navarro E Sánchez-Siles LM Blanco-Navarro I Pérez-Sacristán B 《Journal of agricultural and food chemistry》2011,59(21):11819-11824
Bioactive compounds are used in the design and development of new food products with potential health benefits, although little is known regarding their bioavailability and interactions. This study assessed the stability, in vitro bioaccessibility, and human bioavailability of β-cryptoxanthin from β-cryptoxanthin-rich drinks with and without added phytosterols developed for this purpose. The developed drinks showed no difference in the content of β-cryptoxanthin, and they were stable over 6 months. In vitro, hydrolysis of β-cryptoxanthin esters and the amount of free β-cryptoxanthin at duodenal and micellar phases were similar regardless of the presence of phytosterols. In the human study, the daily intake provoked significant increments of β-cryptoxanthin in serum regardless of the type of the drink. In conclusion, in vitro and in vivo human studies have shown that the bioavailability of β-cryptoxanthin is not significantly affected by the presence of phytosterols when they are simultaneously supplied in a drink. 相似文献
9.
Winfried Schröder Stefan Nickel Simon Schönrock Roman Schmalfuß Werner Wosniok Michaela Meyer Harry Harmens Marina V. Frontasyeva Renate Alber Julia Aleksiayenak Lambe Barandovski Oleg Blum Alejo Carballeira Maria Dam Helena Danielsson Ludwig De Temmermann Anatoly M. Dunaev Barbara Godzik Katrin Hoydal Zvonka Jeran Gunilla Pihl Karlsson Pranvera Lazo Sebastien Leblond Jussi Lindroos Siiri Liiv Sigurður H. Magnússon Blanka Mankovska Encarnación Núñez-Olivera Juha Piispanen Jarmo Poikolainen Ion V. Popescu Flora Qarri Jesus Miguel Santamaria Mitja Skudnik Zdravko Špirić Trajce Stafilov Eiliv Steinnes Claudia Stihi Ivan Suchara Lotti Thöni Hilde Thelle Uggerud Harald G. Zechmeister 《Annals of Forest Science》2017,74(2):31
Key message
Moss surveys provide spatially dense data on environmental concentrations of heavy metals and nitrogen which, together with other biomonitoring and modelling data, can be used for indicating deposition to terrestrial ecosystems and related effects across time and areas of different spatial extension.Context
For enhancing the spatial resolution of measuring and mapping atmospheric deposition by technical devices and by modelling, moss is used complementarily as bio-monitor.Aims
This paper investigated whether nitrogen and heavy metal concentrations derived by biomonitoring of atmospheric deposition are statistically meaningful in terms of compliance with minimum sample size across several spatial levels (objective 1), whether this is also true in terms of geostatistical criteria such as spatial auto-correlation and, by this, estimated values for unsampled locations (objective 2) and whether moss indicates atmospheric deposition in a similar way as modelled deposition, tree foliage and natural surface soil at the European and country level, and whether they indicate site-specific variance due to canopy drip (objective 3).Methods
Data from modelling and biomonitoring atmospheric deposition were statistically analysed by means of minimum sample size calculation, by geostatistics as well as by bivariate correlation analyses and by multivariate correlation analyses using the Classification and Regression Tree approach and the Random Forests method.Results
It was found that the compliance of measurements with the minimum sample size varies by spatial scale and element measured. For unsampled locations, estimation could be derived. Statistically significant correlations between concentrations of heavy metals and nitrogen in moss and modelled atmospheric deposition, and concentrations in leaves, needles and soil were found. Significant influence of canopy drip on nitrogen concentration in moss was proven.Conclusion
Moss surveys should complement modelled atmospheric deposition data as well as other biomonitoring approaches and offer a great potential for various terrestrial monitoring programmes dealing with exposure and effects.10.
Carmen Gómez-Lama Cabanás Encarnación Pérez-Artés 《European journal of plant pathology / European Foundation for Plant Pathology》2014,139(3):445-451
Fusarium oxysporum f. sp. dianthi (Fod) causes vascular wilt, the most important carnation disease worldwide. We have analyzed vegetative compatibility in a collection of Fod isolates, obtained from both soils and carnation plants in the most important growing areas in Spain, by pairing all isolates in all possible combinations. Results showed that isolates of race 1 and race 2 were distributed among three Vegetative Compatibility Groups (VCG) which correlated with the molecular Groups previously described. Isolates of race 1 and race 2 in molecular Group I grouped in VCG 0021, isolates of race 1 type were in VCG 0022, and isolates of race 1 and race 2 in molecular Group II constituted a new VCG (002-), not previously reported. Isolates in each VCG contained the same mating type gene (MAT1-1 or MAT1-2), with the exception of the new VCG 002- that contained both idiomorphs. This work identifies a new VCG in Fod populations and reports for the first time the presence of isolates of race 1 and race 2 in the same VCG. 相似文献