抑制肠道气呼吸大鳞副泥鳅主要呼吸器官的组织病理

为了阐明肠道气呼吸对泥鳅的生理作用,本研究通过抑制大鳞副泥鳅肠道气呼吸,探究其主要呼吸器官的组织病理变化。结果显示,被抑制肠道气呼吸的大鳞副泥鳅通常在1周左右死亡。当被抑制肠道气呼吸的大鳞副泥鳅出现垂死时,采集对照组和实验组的鳃、皮肤、前肠、中肠、后肠以及直肠进行苏木素—伊红(H.E)染色、阿利新蓝—高碘酸雪夫氏(AB-PAS)染色组织切片观察和扫描电镜观察,主要的病理变化:①H.E染色结果显示实验组大鳞副泥鳅鳃丝末端充血,背部皮肤表皮层的毛细血管收缩并减少,且真皮层细胞呈畸形,前肠黏膜褶膨大,后肠浆膜层有血红细胞渗出,后肠、直肠结缔组织显著增厚;②AB-PAS染色结果显示,实验组大鳞副泥鳅鳃、背部皮肤、后肠、直肠组织中嗜酸性空泡细胞均增多,前肠和中肠固有层酸性黏蛋白含量增多,黏膜下层中性黏蛋白含量减少;③扫描电镜结果显示,实验组大鳞副泥鳅鳃丝鳃小片表面皱缩,表皮受损脱落,背部皮肤表面分泌孔增加,中肠内腔表面突起增多,后肠和直肠絮状颗粒增多。研究表明,抑制大鳞副泥鳅肠道气呼吸会引发其主要呼吸器官上皮组织出现黏液细胞增多、血红细胞溢出等病理变化,甚至导致机体死亡,由此可知,肠道气呼吸行为是大鳞副泥鳅的必要生理活动。本研究将为大鳞副泥鳅的健康养殖及幼苗培育提供新的参考。     

The ecosystem services concept: a new Esperanto to facilitate participatory planning processes?

Landscape Ecology - Several case studies investigated the role of ecosystem services in participatory planning processes. However, no systematic study exists that cuts across a large number of...     

Population dynamics of the potato cyst nematode,Globodera pallida,in relation to temperature,potato cultivar and nematicide application

The impact of increasing temperatures on the population dynamics of the soil-dwelling nematode Globodera pallida, a persistent and economically important pest of potatoes, was investigated. The reproductive factor (final population⁄initial population) and length of life cycle were found to be temperature sensitive. Pot experiments performed over 4 months allowed comparison of the effect on development of G. pallida of two temperature regimes: an average temperature comparable to current field conditions (14.3 °C) and an average temperature above current field conditions (17.3 °C). A larger second generation of juveniles was observed at 17.3 °C compared to 14.3 °C. Multiplication of G. pallida at field sites in Shropshire and East Lothian (average soil temperatures of 15.5 and 14.1 °C, respectively, during potato cropping) was also examined. A quantitative PCR assay and visual examination of roots were used to monitor the dynamics of the G. pallida populations in both field sites at 4-weekly intervals. Four cultivars, Desirée, Cara, Maris Piper and Estima, were grown with and without nematicide treatments. Nematicide treatments suppressed population increases at both sites. Females were observed on the roots of cvs Cara and Desirée at the end of the growing season in Shropshire, but not at East Lothian, and are likely to represent a second generation.     

Site‐specific management is crucial to managing Mikania micrantha

Increasingly, weeds have been taking on global distributions. With the proliferation of invasive weeds has come the challenge of managing these species over broad geographical regions, with diverse habitats and political jurisdictions. Here, we review the management of Mikania micrantha Kunth (Asteraceae; mile‐a‐minute) throughout its invaded range, extending through most of the Pacific islands and southern and south‐east Asia. Context matters when determining the best course of action for managing M. micrantha, as it has invaded a large variety of agricultural and natural systems. In Queensland, Australia and Florida, USA, M. micrantha has been targeted in relatively successful eradication campaigns, highlighting the importance of early detection and rapid response methods, while elsewhere in its invaded range, populations are either still increasing or showing limited signs of decline. An inter‐regional approach to research and management should incorporate successful management strategies employed throughout the invaded range including, but not limited to, chemical and cultural control practices, manual and mechanical control, classical biological control using the rust fungus Puccinia spegazzinii, plant–plant competition and integrated approaches utilising two or more control methods concurrently. Additional knowledge of M. micrantha genetics is required to determine if management approaches could be fine‐tuned for particular populations. Countries bordering the Mekong River formed a network in 2011 to co‐ordinate the management of invasive species such as M. micrantha. Expanding such a collaborative approach to other regions could further reduce populations of M. micrantha and limit its spread.     

Naringin-loaded Poly(ε-caprolactone)/Gelatin Electrospun Mat as a Potential Wound Dressing: In vitro and In vivo Evaluation

In the present study, naringin, a flavonoid isolated from the grape and citrus fruit species, was incorporated with poly(ε-caprolactone)/gelatin composite mats in order to develop a potential wound dressing. The composite mats were prepared by electrospinning of poly(ε-caprolactone)/gelatin (1:1 (w/w)) solution incorporated with 1.50 %, 3 % and 6 % (w/w) of naringin. The electrospun mats were evaluated regarding their morphology, contact angle, water-uptake capacity, water vapor transmission rate, tensile properties, drug release, cellular response and in vivo wound healing activity. The study showed that after 2 weeks, the full-thickness excisional wounds of Wistar rats treated with the naringin-loaded dressings achieved a wound closure of higher than 94 % and the dressing containing 6 % (w/w) naringin had almost 100 % wound closure. The sterile gauze, as the control group, showed nearly 86 % of wound closure after this period of time. Our results provided evidence that supports the possible applicability of naringin-loaded wound dressing for successful wound treatment.     

Thresholds,sensitive stages and genetic variability of finger millet to high temperature stress

Finger millet [Eleusine coracana (L.) Gaertn.] is an important coarse cereal crop grown in the arid and semi‐arid regions and often experiences high temperature (HT) stress. The objectives of this research were (i) to quantify effects of season‐long HT stress on physiological and yield traits, (ii) to identify the developmental stages most sensitive to HT stress and (iii) to quantify the genetic variability for HT stress tolerance in finger millet. Research was conducted in controlled environment conditions. HT stress decreased the chlorophyll index, photosystem II activity, grain yield and harvest index. Maximum decrease in number of seeds per panicle and grain yield per plant was observed when stress was imposed during booting, panicle emergence or flowering stages. Maximum genotypic variation was explained by panicle width and number of seeds per panicle at optimum temperature (OT) and grain yield per plant at HT and number of seeds at HT. Based on the stress response and grain yield, tolerant or susceptible genotypes were identified. Finger millet is sensitive to HT stress during reproductive stages, and there was genotypic variability among the finger millet genotypes for number of seeds per panicle and grain yield under HT, which can be exploited to enhance stress tolerance.     

Ecological and landscape effects on genetic distance in an assemblage of headwater fishes

Environmental divergence along hierarchically structured longitudinal gradients may constitute barriers to gene flow in river networks for headwater specialised species. While known, this phenomenon has not been well studied, especially with regard to degree of headwater specialisation. We examined six headwater species that differ in habitat specialisation to assess whether patterns of differentiation vary according to geographic or environmental distance. We also identified regional environmental or anthropogenically induced fragmentation effects by comparing within‐drainage patterns of genetic distance across replicate watersheds. We used a comparative modelling framework to determine whether isolation by distance or isolation by resistance of large river habitats was a better predictor of genetic distance across species. The influence of reservoir presence and regional network characteristics that may influence the hydrology and size of large river habitats were also assessed. Resistance effects from large rivers were closely related to headwater specialisation, with increased specialisation leading to increased resistance and loss of drainagewide population connectivity. These results affirm that dendritic networks naturally fragment headwater specialised species. Further isolation from anthropogenic fragmentation was detected in two of the six drainages, indicating interactions with system‐specific conditions. Landscape variables related to the hydrology of large rivers also affected genetic distance in predicted ways, supporting the importance of large rivers in genetically structuring headwater species in drainage networks.     

Breaking the ice: the introduction of biofouling organisms to Antarctica on vessel hulls

1. Few reports exist that describe marine non‐native species in the Southern Ocean and near‐shore waters around the Antarctic continent. Nevertheless, Antarctica's isolated marine communities, which show high levels of endemism, may be vulnerable to invasion by anthropogenically introduced species from outside Antarctica via vessel hull biofouling.
2. Hull surveys of the British Antarctic Survey's RRS James Clark Ross were undertaken between 2007 and 2014 at Rothera Research Station on the Antarctic Peninsula (Lat. 67°34'S; Long. 68°07'W) to investigate levels of biofouling. In each case, following transit through scouring sea‐ice, over 99% of the vessel hull was free of macroscopic fouling communities. However, in some surveys microbial/algal biofilms, balanomorph barnacles and live individuals of the cosmopolitan pelagic barnacle, Conchoderma auritum were found in the vicinity of intake ports, demonstrating the potential for non‐native species to be transported to Antarctica on vessel hulls.
3. Increasing ship traffic volumes and declining duration of sea ice in waters to the north and west of the Antarctic Peninsula mean the region may be at increased risk of non‐native species introductions. Locations at particular risk may include the waters around popular visitor sites, such as Goudier Island, Neko Harbour, Whalers Bay, Cuverville Island and Half Moon Island, and around northern peninsula research stations.
4. Simple and cost‐effective mitigation measures, such as intentionally moving transiting ships briefly through available offshore sea ice to scour off accessible biofouling communities, may substantially reduce hull‐borne propagule pressure to the region. Better quantification of the risk of marine non‐native species introductions posed by vessel hulls to both Arctic and Antarctic environments, as sea ice patterns and shipping traffic volumes change, will inform the development of appropriate regional and international management responses.

Copyright © 2016 The Authors. Published by Wiley Periodicals, Inc.     

Swim bladder inflation as a possible cause of saddleback-like syndrome malformation in hatchery-reared red spotted grouper <Emphasis Type="Italic">Epinephelus akaara</Emphasis>

Saddleback-like syndrome is one of the most frequently observed malformations in hatchery-reared red spotted grouper Epinephelus akaara. Saddleback-like syndrome is characterized by a V-shaped dorsal-profile depression behind the head region accompanied by deformities or misalignments of the first to fourth neural spines on the vertebral column and the first to fourth pterygiophores of the dorsal fin. Abnormal swim bladder inflation in several finfish species leads to malformations, particularly of the vertebral column. The present study examined the influence of swim bladder inflation on saddleback-like syndrome in hatchery-reared red spotted grouper. The incidence of saddleback-like syndrome increased as swim bladder inflation rate increased. Additionally, the incidence of saddleback-like syndrome among juveniles with inflated swim bladders was significantly higher than that among juveniles with uninflated swim bladders. The larval swim bladder is located just below the first to fifth vertebrae and the first to fifth dorsal spines, where saddleback-like syndrome occurs. Our results suggest that an inflated swim bladder increases the risk of saddleback-like syndrome in hatchery-reared red spotted grouper.