Japanese Quince Seeds as a Promising Rich Source of Proteins and Essential Amino Acids

Plant Foods for Human Nutrition - The Japanese quince (Chaenomeles japonica) seeds (JQS) are generated in large amounts as a by-product in considerable quantities during industrial quince fruit...     

Selected Uncommon Legumes as a Source of Essential Fatty Acids,Tocopherols, Tocotrienols,Sterols, Carotenoids,and Squalene

Plant Foods for Human Nutrition - The seeds of nine species belonging to the Fabaceae family (Cassia alata, Cassia uniflora, Cassia obtusifolia, Cassia tora, Crotalaria albida, Crotalaria juncea,...     

Release of flavonoids from lupin globulin proteins during digestion in a model system

Lupin seed globulin proteins form complexes with flavonoids, predominantly apigenin C-glycosides. Enzymes typical for the gastrointestinal tract were used to hydrolyze lupin seed globulins. Release of native flavonoids as a result of the proteolysis reaction was observed. Different analytical methods such as size exclusion chromatography, HPLC-MS, and fluorescence spectroscopy (steady-state fluorescence, fluorescence anisotropy, fluorescence lifetimes) were used for a detailed characterization of this phenomenon. Flavonoids liberated from lupin globulin proteins as a result of pancreatin-catalyzed digestion were bound by γ-conglutin resistant to this enzyme. Two possible mechanisms of this interaction may be suggested: hydrogen bonding between oligosaccharide chains of glycoproteins and the sugar moieties of the flavonoid glycosides or electrostatic attraction between positively charged γ-conglutin and flavonoids partially ionized at pH 7.5.