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正白术(Atractylodes macrocephala Koidz.)为菊科(Compositae)苍术属(Atractylodes)多年生草本植物,其根茎入药具补脾健胃、燥湿利水、止汗安胎等功效,在我国广泛栽培,并以于术(浙江临安于潜)品质最佳[1]。近年来白术生产由于品种单一,长期连作等因素导致病毒病害日趋严重。据报道,黄瓜花叶病毒(Cucumber mosaic virus,CMV)~([2])、蚕豆萎蔫病毒2号(Broad bean wilt virus 2, 相似文献
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植物通过小干扰RNA(siRNA)介导的RNA干扰机制来防御外来病毒感染,通过对感病植物材料小RNA(sRNA)的深度测序,获得这一过程中产生的与病毒序列高度一致的siRNA序列信息,可以快速地鉴定侵染植物的病毒组成。以采集自浙江省桐乡市桑园疑似感染桑花叶卷叶病(原称桑花叶型萎缩病)的桑树叶片为材料提取总RNA,构建sRNA文库并进行深度测序,对测序得到的总sRNA进行组装后,比对鉴定出桑花叶卷叶病相关病毒(mulberry mosaic leaf roll-associated virus,MMLRa V),并分别得到占MMLRa V 2个组分全基因组14.19%和21.86%的核酸序列。进一步以感病桑叶组织的总RNA为模板,通过RT-PCR扩增获得部分MMLRa V基因组序列,经Sanger测序确定采集的感病桑树叶片中只存在MMLRa V。通过生物信息学方法分析表明,MMLRa V来源siRNA在基因组中的长度分布、5'端碱基偏好性及热点区分布等具有明显特点。研究结果显示,利用siRNA深度测序技术鉴定桑树病毒是非常高效的手段。 相似文献
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Phoebe is a traditional precious tree species in China, which has great economic and ecological value. A new disease twig blight was found in the Phoebe planting area of Zhejiang province. Symptoms usually appear on the base or fork of branches with black swollen epidermis and gradually get worse resulting branch wilting or whole plant death when longitudinal epidermis cracking. The diseased samples were collected in 2017-2018 and the fungal isolate was identified as Lasiodiplodia pseudotheobromae based on morphological characteristics of colony and spore and sequence analysis of ITS region and EF1-α gene. The pathogenicity tests on Phoebe showed that L. pseudotheobromae caused the typical symptoms of twig blight. Koch′s postulates were satisfied following reisolation and identification of the isolate from the infected tissues using the methods described above. This is the first report of Phoebe twig blight caused by L. pseudotheobromae in China. 相似文献
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山核桃干腐病病原菌的鉴定 总被引:1,自引:0,他引:1
葡萄座腔菌科Botryosphaeriaceae真菌是很多木本植物溃疡病和枯梢病的重要病原菌。已有报道认为:山核桃干腐病病原菌为茶藨子葡萄座腔菌Botryosphaeria dothidea,但对于葡萄座腔菌科其他真菌与山核桃干腐病的关系还没有深入研究。分离得到了山核桃干腐病相关病原菌,通过比较其形态学特征和核糖体DNA内转录间隔区(rDNA ITS)序列特征,将分离得到的供试菌株分为3组。根据形态学特征和基于ITS序列的系统发育分析,3组菌株分别鉴定为Botryosphaeria dothidea,B.fabicercianum和B.obtusa。其中B.dothidea为优势菌株,分离频率为71.42%,B.fabicercianum和B.obtusa分离频率分别为14.28%。经致病性测定,上述分离菌株均能引起健康山核桃Carya cathayensis枝条发病,但致病性存在差异。在山核桃上发现的B.obtusa和B.fabicercianum为首次报道。 相似文献