竹花叶病毒浙江麻竹分离物全基因组序列及siRNA分析

正已知有3种病毒可在自然条件下侵染竹类植物,即竹花叶病毒(bamboo mosaic virus,BaMV)~1]、樱桃坏死锈斑驳病毒(cherry necrotic rusty mottle virus,CNRMV)和苹果茎沟病毒(apple stem grooving virus,ASGV)~2,3]。其中,BaMV是最早在巴西的金竹(Bambusa vulgaris Cv.)和孝顺竹

Complete genome sequence and siRNAs analysis of bamboo mosaic virus isolates from Dendrocalamus latiflorus in Zhejiang province,China

Bamboo mosaic virus (BaMV) is an important virus that endangers bamboo plants and causes serious economic losses. In this study, the whole genome nucleotide sequence (MN635746) of BaMV isolated from Zhejiang Dendrocalamus latiflorus (BaMV-ZJMZ) was obtained by small RNA deep sequencing combined with RT-PCR. The genome of BaMV-ZJMZ is appropriate spacing 6 365 nucleotides (nts) long and contains five open reading frames, which is consistent with the structures of already reported BaMV isolates. BaMV-ZJMZ shares nucleotide sequence identities of 82.0%-83.7% with other 20 BaMV isolates at genomic level, and amino acid sequence similarity of CP gene ranging from 91.5% to 95.5%. In phylogenetic tree based on whole genome nucleotide sequences and CP amino acid sequences, BaMV-ZJMZ isolates formed a separate branch. Meanwhile, the small interference RNA (siRNA) derived from BaMV-ZJMZ were analyzed. It was found that the proportions of siRNA from sense and antisense of BaMV-ZJMZ genome were 72% and 28%, respectively, with 21 nt and 22 nt predominated and the 5'-terminal nucleotide base was biased towards "A”, and the most abundant siRNAs were mainly located at the 5'terminal within both positive and negative strands of BaMV-ZJMZ genome. This is the first report of BaMV isolated from Dendrocalamus latiflorus in Zhejiang, which provides important information for further study on genetic evolution and molecular characteristics of BaMV.