Genetic Diversity Analysis of Indonesian Aromatic Rice Varieties (Oryza sativa L.) Using RAPD

Journal of Crop Science and Biotechnology - In this research, we investigated genetic diversity of 21 rice genotypes (Oryza sativa L.) assessed using 38 decamer RAPD primers. A total of 405 bands...     

Genetic and phytochemical analysis of Indonesian black rice cultivars

Journal of Crop Science and Biotechnology - There are many local black rice cultivars spread throughout Indonesia and are only a few of these which formally describe their genetic diversity and...     

Proteomic analysis of wheat flour allergens

Wheat can cause severe IgE-mediated systematic reactions, but knowledge on relevant wheat allergens at the molecular level is scanty. The aim of the present study was to achieve a more detailed and comprehensive characterization of the wheat allergens involved in food allergy to wheat using proteomic strategies, referred to as "allergenomics". Whole flour proteins were separated by two-dimensional gel electrophoresis with isoelectric focusing and lithium dodecyl sulfate-polyacrylamide gel electrophoresis. Then, IgE-binding proteins were detected by immunoblotting with sera of patients with a food allergy to wheat. After tryptic digestion, the peptides of IgE-binding proteins were analyzed by matrix-assisted laser desorption ionization tandem time-of-flight mass spectrometry. In this study, we identified four previously reported wheat allergens or their sequentially homologous proteins [serpin, alpha-amylase inhibitor, gamma-gliadin, and low molecular weight (LMW) glutenin] by a database search. As a result of the high resolution of two-dimensional gel electrophoresis, nine subunits of LMW glutenins were identified as the most predominant IgE-binding antigens. The two-dimensional allergen map can be beneficial in many ways. It could be used, for example, for precise diagnosis of wheat-allergic patients and assessment of wheat allergens in food. Additionally, we compared allergenomics to conventional biochemical methods and evaluated the usefulness of a proteomic strategy for identifying putative allergens to wheat allergy.     

Expression of somatic embryogenesis-related genes in sugarcane (Saccharum officinarum L.)

Journal of Crop Science and Biotechnology - The low frequency of plantlet regenerates from the somatic embryogenesis (SE) callus in sugarcane becomes a problem to produce its seed. Plant growth...     

Impact of transgenic sugarcane overexpressing SoSPS1 gene on bacterial diversity,enzyme activity and minerals content in soil rhizosphere

Journal of Crop Science and Biotechnology - The transgenic sugarcane overexpressing SoSPS1 gene increased sugar content which may alter the root exudation that interact with soil rhizosphere...     

Diversity,Physicochemical, and Structural Properties of Indonesian Aromatic Rice Cultivars

Journal of Crop Science and Biotechnology - The most important component of rice qualities are its appearance, cooking quality, eating quality, and nutritional quality. Indonesian had been...     

Callus Induction and Regeneration from Anther Cultures of Indonesian Indica Black Rice Cultivar

Journal of Crop Science and Biotechnology - The assembly of superior varieties and collection of rice germplasm involves the process of selecting and storing elders that have superior genotypic...