耐低温耐低氧萌发野败不育系赣野A的选育

赣野A是江西省农科院水稻研究所将东乡野生稻的耐冷性和耐低氧萌发能力导入抗稻瘟病保持系赣香B后再与赣香A测交和回交育成的野败型三系籼稻不育系。该不育系败育彻底,异交结实率高,配合力较强,具有较强的耐冷性和耐低氧萌发能力,在直播杂交稻育种中应用前景广阔。2019年通过了江西省品种审定。     

鸡传染性贫血病毒VP1、VP2基因在杆状病毒表达系统中的表达

将鸡传染性贫血病毒M9905株VP1、VP2基因分别或同时克隆到杆状病毒转移载体pFastBacDUAL中，然后转化到DHIOBAC感受态细胞中与Bacmid杆状病毒穿梭载体进行转座重组，最后将重组子转染Sf9昆虫细胞，得到分别或同时含VP1、VP2基因的重组杆状病毒rBacVP1、rBacVP2及rBacVP1—2。PCR扩增结果证实VP1、VP2基因重组到杆状病毒基因组中；SDS-PAGE电泳分析和间接免疫荧光试验结果表明VP1、VP2基因在重组病毒中得到了表达。     

利用PCR对鸭瘟和鸭"传染性肿头症"的鉴别诊断

本文利用聚合链反应(PCR)技术对鸭瘟和鸭“传染性肿头症”进行鉴别诊断。结果证明该方法具有特异、快速的特点。可对这两种临床症状较为相似的鸭传染性疾病进行快速鉴别诊断。     

大豆新品种黑农44的选育及不同种植方式对其产量和品质的影响

黑农 4 4属于高脂肪、高产品种 ,其基础与亲本的遗传有关 ,在后代选择上主要是按着母本哈85 - 6 437(黑农 37)生态类型选择。不同密度穴播比条播增产 ,在 15～ 2 5万株 /hm2 时穴播较条播增产 0 .9%～ 13.1% ,同时脂肪含量提高 ;黑农 4 4主要栽培要点是 :密度 2 0～ 2 5万株 /hm2 ,采用穴播 ,种肥施磷酸二铵 15 0～ 187.5kg/hm2 ,实行通透管理 ,防治病虫草害。     

家禽核酸疫苗的研究进展

核酸疫苗是当今疫苗研究的最前沿领域，本文就核酸疫苗在家禽传染病中的应用做一综述。     

鸭瘟诊断方法研究进展

鸭瘟是由疱疹病毒引起的鸭、鹅的一种烈性传染病。目前已有多种诊断方法用于鸭瘟的诊断。本文就鸭瘟诊断方法的研究进展作一综述。     

Inhibitory effects of LDL-ACM complex on subcutaneous implanted tumors in nude mice()

AIM: In order to evaluate the applicable value of LDL as a targeted vehicle for chemotherapeutic agents, we investigated and compared the inhibitory effects of LDL-ACM complex and free ACM on nude mice's subcutaneous implanted tumors derived from gastric cancer cell lines, SGC-7901 and NKM-45. METHODS: LDL-ACM complex was prepared and the tumor model of nude mice was established by subcutaneous implantation of SGC-7901 and NKM-45. Then, the groups of nude mice developed subcutaneous implanted tumors were received either LDL-ACM complex or free ACM. Subsequently, the tumor size, weight and leukemia cell counts were measured and the rates of tumor-inhibition and the survival were compared among the groups. RESULTS: The inhibitory effects of LDL-ACM complex on the tumors, especially on SGC-7901 implanted tumors were much more obvious than that of free ACM. It was also indicated that the action of LDL-ACM complex was mediated by LDL receptor. CONCLUSION: These results showed that LDL-ACM complex had significant inhibitory effects on the implanted tumors and the effect might be mediated by LDL receptor.     

中国苜蓿繁育概述

回顾了建国以来国内苜蓿的育种发展状况,进行了苜蓿的种质评价与筛选,地方品种的系统化,育成了抗寒新品种草原1号、草原2号,抗病品种中兰一号,耐盐品种中苜一号,耐牧根蘖品种公农3号、中图3号、中图4号,高产的公农1号等。提出了在我国苜蓿育种方法上,杂交育种与基本育种方法在当代育种中将继续存在,生物技术在育种中的应用将加快育种进程;指出了我国苜蓿育种存在的问题是育种体系不健全,育种手段方法落后,抗性育种缓慢,对引入品种缺少理论与实践的鉴定、评价,国内育成品种少,高层知识力量薄弱等。     

稻-鸭生态系统中二化螟消长动态研究

2003年4～10月,在稻鸭共栖田中,对二化螟发生规律的系统研究结果表明,稻田养鸭能显著减轻二化螟的为害。与对照相比,中稻田养鸭区二化螟为害株率减少13.4%～47.1%;晚稻田减少62.2%。稻鸭共栖能减少化学农药的使用量,具有较好的经济、社会和生态效益。     

Cortical gene expression pattern in rat focal cerebral ischemia

AIM: To investigate the genes differential expression in cortex during rat focal cerebral ischemia.METHODS: cDNA microarray chips containing numerous cDNAs were used to investigate the gene expression pattern between samples of focal cerebral ischemia and sham-control operation rats. RESULTS: Two hundred and eleven genes differentially expressed were screened out, among these genes, up-and down-regulated genes were 199 and 12, respectively. CONCLUSIONS: The analysis of gene expression pattern of focal cerebral ischemia based on cDNA microarray can realize high-throughput screening of the genes associated with the focal cerebral ischemia. The differential expression of genes may be related to the pathogenesis of focal cerebral ischemic diseases.