不同生育阶段燕麦对干旱胁迫的响应

设中度干旱(MD,土壤田间最大持水量的55±5%)、重度干旱(SD,土壤田间最大持水量的35±5%)两个处理,研究干旱胁迫对温室盆栽燕麦不同生育阶段(苗期、分蘖期、拔节期)的生长性状、叶绿素荧光参数以及根系形态的影响。结果表明,与正常供水(CK)相比,干旱胁迫处理均显著(P<0.05)降低了燕麦苗期、分蘖期、拔节期3个生育阶段地上部生物量、株高以及叶片光合荧光参数Fv/Fm、Fv/Fo。干旱胁迫下,燕麦根系表现出明显的形态可塑性,苗期通过增加根长、分支数和细根数(直径≤0.48mm)来响应干旱胁迫,分蘖期主要通过减少根长、分支数来响应干旱胁迫,拔节期主要通过减缓各级根系的生长并增加根的分级数来响应干旱胁迫。     

不同有机肥氮替代化肥氮比例对马铃薯根系吸收能力和形态的影响

针对甘肃定西地区马铃薯生产长期大量施用化肥造成马铃薯产量降低、品质下降以及环境污染等问题,通过大田试验,研究了不同有机肥氮替代化肥氮比例处理对旱地覆膜马铃薯根系吸收能力和形态的影响。结果表明: 不同施用量有机肥氮替代化肥氮处理显著增加了马铃薯根系活力、活跃吸收面积和总吸收面积,且随着替代比例的增加逐渐增加,在有机肥氮替代化肥氮比例达到30%时达到最大值,而后降低;不同施用量有机肥氮替代化肥氮处理对马铃薯根系总根长、根体积、总根尖数和主根直径有显著影响,特别是增加了0~0.50、0.51~1.00和1.01~1.50mm直径范围内马铃薯根系的总根长;施用有机肥的处理显著增加了马铃薯块茎产量,与纯施化肥相比分别提高了11.32%、16.04%、23.53%和12.69%。综上所述,在定西地区,施氮量为纯氮180kg/hm²,氮、磷、钾配比为4:3:3（N:P₂O₅:K₂O）,有机肥氮替代化肥氮比例为30%是最佳施肥模式。     

人工模拟增雨对油蒿种子形态性状和比叶面积的影响

【目的】研究油蒿的种子大小和比叶面积对不同增雨处理的响应,探明两者之间的关系,以期揭示油蒿植物功能性状对降水量变化的响应机制和演变趋势。【方法】根据内蒙古磴口县多年平均降水量,在植物生长季5—9月设计4个增雨梯度(25%,50%,75%和100%),对天然油蒿灌丛进行增雨试验,利用Winseedle种子和针叶图象分析软件系统,对比了油蒿种子表型形态特征和比叶面积对降水增多的响应,同时通过相关分析研究了油蒿种子大小与比叶面积的关系。【结果】不同增雨量对油蒿种子长度、宽度、体积、表面积及千粒重均有显著影响,在100%增雨处理下种子各性状指标达到最小值,其中种子宽度、体积、表面积与增雨量呈极显著负相关(P<0.01)。种子长度和千粒重与增雨量呈显著负相关(P<0.05),种子长宽比与增雨量的相关性不显著(P>0.05)。不同增雨量对油蒿比叶面积的影响差异性显著(P<0.05),且随着雨量的增加,比叶面积不断增大。种子大小与比叶面积呈负相关关系,但均未达到显著水平(P>0.05)。【结论】降水增加后油蒿种子会先增加后减小来适应环境变化,通过油蒿种子、比叶面积两大植物功能性状组合对增雨量处理响应的研究体现了荒漠植物应对未来气候变化的生存策略和适应机制。     

Isolation of Streptococcus cuniculi from corneal lesion in laboratory-raised mice

Corneal lesions appearing as white mass beneath intact epithelium, with ocular discharge in one mouse, was observed in a batch of laboratory-raised BALB/c mice (n=9 of 56). The affected mice remained active, well-groomed and had normal appetite. Isolates recovered from swab cultures of the external and internal contents of the eye had partial 16S rRNA gene sequence of 99.1% similarity to Streptococcus cuniculi. No previous report of S. cuniculi infection in laboratory rodents has been presented. The isolate was susceptible to all antibiotics tested. We suggest S. cuniculi is an opportunistic bacteria in laboratory mice but are uncertain of its source. Our findings revealed that S. cuniculi is able to colonize laboratory mice and should be considered when mice present with eye lesion or ocular discharge.     

Effect of apple cultivars and storage periods on the virulence of Neofabraea spp.

Bull’s eye rot is a typical quiescent postharvest apple disease in major fruit-growing areas. The susceptibility of different apple cultivars to Neofabraea spp. (N. vagabunda and N. malicorticis) was assessed, with Granny Smith showing the most resistance and Cripps Pink the most susceptibility. To assess the factors involved in conidial germination, Neofabraea spp. were grown on crude protein extracts (CPEs) collected from apple fruits at different storage periods. Fungal germ tube growth rate and pathogenic enzyme (cellulase and xylanase) activity were assessed. Results showed that CPEs collected after 2 and 4 months of storage progressively stimulated conidial germination and germ tube elongation, while a lesser effect was observed from CPEs after 1 month of storage. Xylanase proved to be the main degrading enzyme secreted by all the isolates, while cellulase was produced only by N. vagabunda isolates. Overall, the isolate ID02 was the most virulent, based on more rapid germ tube elongation and greater activity of the lytic enzymes.     

甜瓜质地差异及相关细胞壁酶活性变化

对两种不同质地类型甜瓜果实发育过程中果肉质地的变化及相关酶活性进行研究。结果表明,花后35~40 d为软、脆两种甜瓜质地形成的关键时期。在该时期,软肉型甜瓜‘NSL’细胞面积与间隙增大,果肉细胞排列疏松,细胞面积为脆肉型甜瓜的115.35%,软肉型甜瓜‘NSL’与脆肉型甜瓜‘XZM’质构参数差异显著,脆肉型甜瓜果实4种细胞壁酶活性总体低于软肉型甜瓜。花后35 d,软肉型甜瓜‘NSL’细胞壁扩展酶基因(CmEXP3、CmEXP5、CmEXP9)相对表达量为最大值,显著高于脆肉型甜瓜‘XZM’。     

金寨毛竹纤维形态及化学成分

通过离析法制取毛竹纤维,观察并记录纤维长度和宽度,计算纤维长宽比,按照国家标准分别测定化学成分质量分数,以此对3~5年生金寨毛竹不同竹干高度、径向的纤维形态和化学成分进行研究。结果表明:3~5年生金寨毛竹平均纤维长度均大于1500μm,且随着竹龄的增加而增加,而在高度上变异规律不明显;平均纤维长宽比均大于100,在竹干高度为5.5 m处达到最大值;灰分质量分数随竹龄的增加而减小,随竹干高度的增加而增加;综纤维素质量分数随竹龄的增加而增加,随竹干高度的增加趋于稳定;苯醇抽提物和纤维素质量分数随竹龄增加先减小再增加。综上可知,金寨毛竹是优良的造纸原料。     

Morphological differences among Raphanus raphanistrum populations and their relationship to related crops

Wild radish (Raphanus raphanistrum) has developed introgressed populations after hybridization with its cultivated counterpart (R. sativus) in California. Hybridization with various Brassica and Sinapis species is also possible. To determine if hybridization is responsible of the genetic diversity of European populations, six wild radish populations with distinct morphological traits were sampled from geographically distant regions in Europe. Plants were cultivated in an oilseed rape field and in insect‐proof cages. Silique and flower morphology, growth, and reproductive traits were measured. The wild radish populations could be discriminated by the morphological traits, but not related to geographic regions. In particular, populations of one region showed wide variability in terms of silique shape and growth behaviour, and small‐sized flowers. Although the origin of morphological diversity in wild radish is unclear, i.e. native or due to gene flow from the cultivated radish or other Brassicaceae, significant morphological divergence was found that could have relevant effects on plant ecology and adaptation.     

混合盐胁迫对马蔺幼苗根系构型及生长量的影响

为探讨不同种类盐胁迫对马蔺幼苗生长状况的影响,采用室内培养的方法,研究总质量浓度为0.3%的NaCl、NaHCO₃单盐溶液及其不同配比的混合盐溶液胁迫对马蔺(Iris lactea var. chinensis)幼苗生长量、地上部干鲜重比值、地下部干鲜重比值以及根系构型的影响。结果表明,随着胁迫时间的延长,0.3%NaCl溶液对叶数和株高增长量的影响最小,其次为3种混合盐溶液,各处理在第14天时株高增幅达到最大。经0.3%NaHCO₃处理后的幼苗地上部干鲜重的比值为空白对照的2.17倍,地下部干鲜重则为空白对照的56.61%,随着混合溶液中NaHCO₃浓度的上升,地上部干鲜重比值表现出上升的趋势,地下部干鲜重比值则表现出下降的趋势。经35天处理后,0.3%NaHCO₃胁迫的马蔺幼苗根系总长、根系表面积较对照均显著下降,降幅分别为39.73%、26.08%,而各处理间的根系体积却无显著差异。研究结果显示,0.3%NaHCO₃溶液对马蔺幼苗的胁迫作用最强,2种单盐对根系的胁迫作用强于混合盐胁迫,而0.3%NaCl溶液对生长量的胁迫作用最弱。