杉木第3代种子园多元素配方施肥效应研究

杉木种子园为培育高产稳产杉木良种提供重要保障,在林业生产中具有积极意义。通过试验研究,探究杉木3代种子园土壤养分丰缺情况、养分间的平衡规律,确定杉木种子园最佳肥料配比和施肥量,以期为闽北低山丘陵红壤分布区杉木种子园提供推荐施肥配方。采用“3414”配方施肥设计方案,对闽北第3代杉木种子园开展N、P、K、Ca、Mg、B、Mo等元素施肥试验。分别设计大量元素氮、磷、钾三因素施肥水平（并增设施用钼肥处理）、中微量元素钙、镁、硼三因素施肥水平,以球果数量（质量）、出籽率、种子产量、胸径等为评价指标,拟合肥料效应函数方程,研究各养分间的作用规律,并提出最佳施肥配比和施肥量。结果表明：种子园土壤N、P、K、Ca、Mg养分含量较低,B含量属于中等水平,而Mo含量较高;N、P、Mg以及N-K、Ca-B肥料联合对种子园产量的影响达到显著或极显著水平,而单施某一肥料效应较小,其中单施K、Ca、B几乎没有效果,而肥料联合施用效果较好。综合效应分析结果,得出种子园最大产量施肥量组合为：每株施尿素100 g+过磷酸钙897 g+氯化钾150 g+石灰150 g+硫酸镁105 g+硼砂75 g,并配施钼肥。N、P₂O₅、K₂O、CaO、Mg、B、Mo的用量依次为46.0、107.7、90.0、75.0、31.4、2.3、5.0 g,其配比为N:P₂O₅:K₂O:CaO:Mg:B:Mo=1.0:2.3:2.0:1.6:0.7:0.05:0.1。多元素配方施肥能很好地改善杉木种子园产量和质量,单株球果产量、球果单粒重、出籽率及种子园产量等指标均比不施肥的母树有大幅度提高。本研究施肥方案,理论上种子园产量可达45.99~95.65 g/株,大量元素施肥试验的产量达到理论产量的80%~86%,中微量元素施肥试验的产量为理论产量的70%左右。现实种子园平均产量大约是预测产量的40%,通过合理配方施肥有望大幅度提高杉木3代种子园产量。     

Versuche zur Zyklussynchronisation bei Kühen aus Beständen rnit Fertilitätsproblemen und bei Hybriden zwischen Wisent und Rind mit der PRID-Spirale*

Inhalt: An 81 Versuchs- und 92 Kontrolltieren der Rasse “Potnische Schwarzbunte” aus 3 Problembetrieben wurde ein Versuch zur Sterilitätsprophylaxe rnit der PRID-Spirale durchgeführt. Anhand klinischer Befunde und Milchprogesteronwerten wurde die Untergruppe aus Kühen mit Ovardystrophie (18 Versuchs- und 22 Kontrolltiere) gebildet und extra ausgewertet. Der Behandlungsbeginn lag zwischen 60 und 80 Tage post partum. Die Versuchstiere wurden mit der PRID-Spirale 12 Tage lang behandelt, die Kontrolltiere erhielten Injcktionen rnit Kochsalzlösung und solche rnit Ovardystrophie wurden zusätzlich mit einer Eierstocksmassage behandelt. Die Versuchstiere wurden 56 und 72 Stunden nach Entfernung der Spirale blind besamt. Die Brunstin-duktionsrate betrug insgesamt 90,1%, bei denen rnit Ovardystrophie 77,7%. Die An-wendung der PRID-Spirale führte zur Verbesserung der Fertilitätslage. Die Gesamt-trächtigkeitsrate lag mit 87,6% bei den Versuchskühenüber derjenigen der Kontrollkü-he mit 79,3%. Die Behandlung der Tiere rnit Ovardystrophie erbrachte eine Gesamt-trächtigkeitsrate von 88,8% bei den Versuchs- und 72,7% bei den Kontrolltieren. Die Zwischentragezeit betrug bei den Versuchstieren 101,2 Tage und bei den Kontrolltieren 113,3 Tage (p ≤ 0,05). Bei den azyklischen Tieren lag die Zwischentragezeit bei 104,6 Tage bzw. 134,7 Tage (p ≤ 0,05). In einem anderen Versuch wurde 20 Hybriden zwischen Wisent und Hausrind rwecks Zyklussynchronisation die PRID-Spirale verabreicht. Die Brunstinduktionsrate betrug 66,6%, das Erstbesamungsergebnis nach Doppelbesamung 30%, die Gesamtträchtig-keitsrate nach dem Decken der umrindernden Kreuzungstiere rnit einem Bullen 90%. Die Deckperiode konnte verkürzt werden. Contents: Investigations on the synchronization of estrus cycle in cattle from farms with fertility problems and in cross-breeds between bison and cattle with a PRID-de-vice In 81 experimental and 92 control animals of the “Polish Black and White” breed derived from 3 farms with fertility problems an investigation was carried out for sterility prophylaxis using the PRID-device. Based on clinical findings and milk progesterone values cattle with ovarian dystrophy (18 experimental and 22 control animals) from a special group were used. The begin of treatment was between 60 and 80 days post partum. All experimental animals had received a PRID-device for a duration of 12 days. Control animals were injected with physiological saline solution and animals with ovarian dystrophy were treated additionally with ovarian massage. Experimental animals were inseminated 56 and 72 hrs after removal of the device. All animals taken, estrus was induced in 90.1% of the animals, while only 77.7% of animals with ovarian dystrophy came to estrus. Application of the PRID-device led to an improvement of fertility. Pregnancy rates in experimental animals were 87.6%, while only 79.3% of the control animals were pregnant. Treatment of animals with ovarian dystrophy resulted in 88.8% pregnancies for treated and 72.7% for control animals. Experimental animals were non-pregnant for a duration of 101.2 days, while control animals remained non-pregnant for 113.3 days (p < 0.05). Acyclic cattle had a non-pregnant period of 104.6 days and 134.7 days (p < 0.05) respectively. In a second experiment 20 cross-breeds between bison and domestic cattle were treated with a PRID-device for synchronization of the estrus cycle. The rate of estrus induction was 66.6%, the fertility after double insemination 30%, the total pregnancy rate improved after mating with a bull of animals that had not conceived to 90%.     

牧压和肥力对亚热带山地牧草净生产的影响

在亚热带山地湿润环境条件下,研究牧压和土壤肥力对人工草地净生产的影响.三年试验结果表明,绵羊在轮牧下,产草量和磷肥对全年及各月牧草净生产具有显著影响.禁牧时,夏季地上生物量积累到一定程度后,只有很少量的净生产.放牧时,产草量在1000 kg DM/hm2左右时,净生产最大,当产草量低于300 kg DM/hm2以下时,净生产量则降低.每年施一次P2O5 36～60 kg/hm2,可以显著增加牧草净生产,尤以春末夏初时施肥更为显著.最低产草量出现在不施肥处理.     

草地土壤肥力及其营养管理的必要性

介绍了草地土壤肥力的来源,土壤基本养分对草地生产的作用,草地植物与动物对养分的需求量及缺乏症状,说明了加强草地营养管理以及维持草地生态系统营养平衡的重要性,指出增强对土壤肥力和营养管理是提高草地生产能力、保证草地生态系统平衡、保障草地可持续利用的关键技术措施。     

春麦田除草剂的应用与杂草群落演替

作者采用定点定位试验,对春麦田除草剂应用与杂草群落演替趋势进行研究。结果表明:在杂草群落多样性麦田,小麦连作并分别连续5年施用同一种除草剂,原杂草群落中占优势的靶标杂草得以控制,而非靶标杂草和抗、耐药性杂草因失去竞争和制约对象而猖獗发展,发生量较原来增加几倍至几十倍,并形成优势种群,对小麦造成新的更严重的危害。一种除草剂在同田块连续施用4年,由于杂草群落演替,抗、耐药杂草兴起,除草效果显著下降而失去其使用意义。作者提出除草剂配套使用、轮用、混用等措施,并配合以合理的轮作制度,以减轻杂草群落长期受到单一的定向选择性压力。     

基于GGE双标图对苜蓿品种丰产性和稳定性综合评价

为了解不同苜蓿（Medicago sativaL.）品种在宁夏南部山区的丰产性、稳产性，以及试点的代表性和区分力，本研究利用方差分析、GGE双标图模型对区域3个不同试点的6个苜蓿品种的产量进行分析。结果表明：苜蓿产量最高的试验区是原州区彭堡镇彭堡三队，最低的是隆德县关庄乡前庄村，前者比后者高43.7%；产量最高的品种是‘甘农7号’，最低的是‘阿尔冈金’，前者比后者高44.9%；联合方差分析显示，基因型与环境互作（G×E）、环境（E）、基因型（G）对品种产量影响显著（P<0.05），其中G×E是引起苜蓿产量差异的最主要原因，它引起的变异是E的1.19倍，是G的1.58倍。利用GGE双标图模型分析可知，高产稳产的品种是‘三得利’、‘苜蓿王’；3个不同区域可划分为2个生态区，原州区头营镇徐河村和原州区彭堡镇彭堡三队为1个，隆德县关庄乡前庄村为1个；综合代表性和区分力，较理想的试点为头营镇徐河村。     

不同肥力水平下种植密度对浚单29产量性状及产量的影响

[目的]探寻浚单29不同肥力水平地区的适宜种植密度。[方法]研究在高产田与一般田2种肥力水平下浚单29种植密度与产量及其相关产量性状的关系。[结果]2种肥力水平下通过种植密度变化影响穗部性状来影响产量,相同密度,一般田肥力水平下,密度对穗部性状影响大于高产田对穗部性状影响。[结论]在一般田肥力水平下,浚单29最适宜密度为6.75万株/hm2,高产田肥力水平下,浚单29最适宜密度为7.50万株/hm2。     

海南荔枝主产区果园土壤养分状况分析

调查分析海南荔枝主产区荔枝园土壤养分状况,为荔枝的养分管理与合理施肥提供理论依据和科学指导。选择具有一定种植规模、有代表性的荔枝园 49 个,采集0~30 cm土层土壤样品进行养分丰缺状况的测试分析各种性质。结果表明,海南荔枝园土壤pH大部分呈酸性,16.3 % 荔枝园土壤pH适宜荔枝生长,土壤阳离子交换量为中等水平,63.3 % 果园土壤有机质含量处于适宜值及以上;土壤全氮、碱解氮、有效磷、速效钾含量丰富;土壤交换性钙、有效硫含量丰富,42.7 % 果园土壤交换性镁含量缺乏,4.1 % 果园极缺乏;土壤微量元素铁、锰、铜、锌、钼含量较为丰富,但73.5 % 果园土壤有效硼含量低于适宜值。不同产区荔枝园土壤养分状况存在差异,海口果园土壤有机质和有效养分氮、钾、钙、镁含量最高,约2/3产区果园土壤偏缺交换性镁,仅海口、澄迈、定安土壤交换性镁含量在适宜值以上,各产区荔枝园土壤有效铁、锰、铜、锌、钼微量元素丰富,土壤有效硼在琼海果园含量丰富,澄迈、万宁、陵水、儋州、白沙荔枝园土壤有效硼偏缺。综合分析可知,各产区土壤肥力由高到低依次为：海口>定安>澄迈>文昌>琼海>儋州>万宁>陵水>白沙。综上所述,海南荔枝园应根据土壤养分情况平衡施肥,改良土壤酸度,增施有机肥,重视镁、硼肥施用,适当减施化肥以获得更好的产量效应。     

Quality of Fresh,Cooled, and Frozen Semen From Stallions Supplemented with Antioxidants and Fatty Acids

This study assessed the effect of oral supplementation with the primary antioxidants and fatty acids involved in spermatogenesis (L-carnitine, selenium, vitamin E, omega-3, and omega-6) on the seminal quality in fresh, cooled, and frozen semen of stallions (n = 8), using a randomized design. The animals were divided into Group I (n = 4) and Group II (n = 4) for a 30-week experiment. The two groups alternated between nutraceutical supplementation and a placebo over the course of the experiment. Semen collections were performed in two sets: once in the middle of the experiment, before the two groups switched treatments, and once at the end. The volume, appearance, sperm concentration, spermatozoa kinetics, and membrane integrity of fresh semen were evaluated. The spermatozoa kinetics and membrane integrity of cooled (for 24, 36, and 48 hours) and frozen semen were also evaluated. No differences were observed in volume, appearance, and sperm concentration between treatment and control. However, compared with placebo, nutraceutical supplementation increased (P < .05) total motility, trajectory speed, as well as plasma and acrosomal membrane integrity in spermatozoa from fresh semen. In cooled semen, nutraceutical treatment also increased (P < .05) total motility, speed, and membrane integrity of spermatozoa compared with the control. In frozen semen, supplementation increased (P < .05) spermatozoa progressive motility and plasma membrane integrity. Our results suggest a positive, synergistic effect of the antioxidant L-carnitine and selenium on spermatozoa kinetics. Similarly, the increase in plasma and acrosomal membrane integrity could be attributed to higher concentrations of polyunsaturated fatty acids (a key cell-membrane component), combined with the prevention of excess lipid peroxidation by antioxidants. In conclusion, supplementation with nutraceuticals containing fatty acids and antioxidants improved the quality of fresh, cooled, and frozen stallion semen. Therefore, nutraceutical use should increase the success of artificial insemination with cooled and cryopreserved semen.