碎甘薯秧离散元仿真参数测量与标定*

针对甘薯秧蔓机械化回收过程中离散元仿真研究缺乏准确参数值的问题,采用直接测量和虚拟标定相结合的方法对碎甘薯茎秆和叶片离散元仿真参数进行研究。采用物理试验法获得碎甘薯秧的本征参数、碰撞恢复系数等参数值及碎甘薯秧颗粒的静摩擦系数参数范围,并为离散元法仿真设计了不同的参数组合。通过堆积角优化仿真试验确定甘薯叶片本征参数及其他不易直接测量的离散元仿真参数。Plackett-Burman试验表明,甘薯茎秆—甘薯茎秆和甘薯茎秆—45钢的静摩擦系数、甘薯茎秆—甘薯茎秆和甘薯茎秆—甘薯叶的滚动摩擦系数均显著影响堆积角。运用最陡爬坡试验和Box-Behnken优化试验标定了对碎甘薯秧堆积角有显著影响的参数值,以得到的参数进行颗粒堆积仿真试验,测得堆积角平均值为40.51°,与实测值相对误差为0.972%,说明物理试验加优化仿真试验来标定离散元参数是可行的,标定所得的参数可作为甘薯秧茎叶离散元仿真参数。     

马铃薯致病疫霉拮抗细菌研究进展

为了解决马铃薯致病疫霉侵染马铃薯导致其死亡,进而造成马铃薯单位种植产量低的问题。本文从拮抗菌入手,归纳分析细菌类拮抗菌中的主要拮抗菌中芽孢杆菌、假单胞菌和粘细菌对马铃薯致病疫霉的抑制作用。同时,在总结前人研究的基础上,笔者认为在拮抗菌应用方面,可以采用多对一的新型平板对峙筛菌模式来模拟复杂条件下拮抗菌的拮抗作用,以期得到稳定的拮抗菌;并指出应从定植能力和根际微生物影响方面对拮抗菌实际应用的可行性进行研究;此外,提出未来对拮抗菌抑制马铃薯致病疫霉的探究应从表型层面的研究深入到分子层面,以期能成功从源头找寻抑制马铃薯致病疫霉的答案。     

欧美国家针叶树容器苗生产与启示

针叶树是芬兰、瑞典、挪威、美国、加拿大等欧美国家主要的商品林树种。容器苗是针叶树重要的育苗类型。与裸根苗比较，容器苗具有育苗周期更短、造林季节可延长、苗木适应性更广、苗木质量更一致的优点。因此，容器育苗成为精准林业发展的必然趋势。为了借鉴欧美容器育苗的技术方法，提高我国容器苗的质量，为我国针叶树容器苗发展提供参考，文中主要从4个方面综述了欧美国家检测与提高苗木质量的方法，即列举了针叶树常用的苗木质量检测指标，总结了育苗容器与育苗基质的改良进展，介绍了养分、水分、光照与温度等培养条件的优化手段，简要概述了容器苗病虫害防控的进展，提出了我国相关树种容器苗生产的启示与借鉴。     

Temporal and spatial progress of the diseases caused by the crinivirus tomato chlorosis virus and the begomovirus tomato severe rugose virus in tomatoes in Brazil

Efficient management of whitefly-borne diseases remains a challenge due to the lack of a comprehensive understanding of their epidemiology, particularly of the diseases tomato golden mosaic and tomato yellowing. Here, by monitoring 16 plots in four commercial fields, the temporal and spatial distribution of these two diseases were studied in tomato fields in Brazil. In the experimental plots these diseases were caused by tomato severe rugose virus (ToSRV) and tomato chlorosis virus (ToCV), respectively. The incidence of each virus was similar in the plots within a field but varied greatly among fields. Plants with symptoms for both diseases were randomly distributed in three of four spatial analyses. The curves representing the progress of both diseases were similar and contained small fluctuations, indicating that the spread of both viruses was similar under field conditions. In transmission experiments of ToSRV and ToCV by Bemisia tabaci MEAM1 (former biotype B), these viruses had a similar transmission rate in single or mixed infections. It was then shown that primary and secondary spread of ToCV were not efficiently controlled by insecticide applications. Finally, in a typical monomolecular model of disease progress, simulation of the primary dissemination of ToSRV and ToCV showed that infected plants were predominantly randomly distributed. It is concluded that, although the manner of vector transmission differs between ToSRV (persistent) and ToCV (semipersistent), the main dispersal mechanisms are most probably similar for these two diseases: primary spread is the predominant mechanism, and epidemics of these diseases have been caused by several influxes of viruliferous whiteflies.     

马铃薯枯萎病和干腐病药剂防治筛选试验报告

马铃薯产业是宁夏“1+4”特色优势产业,是西吉县所有农作物中种植面积最大、涉及农户最多、比较效益最高的农民脱贫致富的主导产业。近年来西吉县马铃薯产业由商品薯种植大县向种薯繁育大县的转变,推动了农业增效,农民增收的快速发展。但各类土传性病害的发生影响了马铃薯产业的发展,同时磋商了农民种植的积极性。为了测定筛选出对马铃薯枯萎病和干腐病防治效果好、成本较低药剂,为大田防治提供科学依据。     

Comparison of flagellin and an oil-emulsion adjuvant in inactivated Newcastle disease vaccine in stimulation of immunogenic parameters

The present study was designed to investigate the potential application of native (N) and recombinant (truncated modified [tmFliC] and full-length [flFliC]) flagellin proteins along with inactivated Newcastle disease virus (NDV). Fifty six SPF chickens were immunized twice with PBS (control), inactivated NDV (Ag), inactivated NDV/flFliC (AgF), inactivated NDV/tmFliC (AgT), inactivated NDV/N (AgN), commercial vaccine containing Montanide (Vac) and Vac/N (VacN), with a two-week interval. Blood was collected weekly and spleens were harvested after chickens were sacrificed. Interleukin-6 (IL-6) and tumor necrotic factor-α (TNF-α) gene expression in peripheral blood mononuclear cells were analyzed by Real-Time PCR. Antibody response was assessed by haemagglutination inhibition (HI). Cellular activity was quantified by MTT assay. Results showed that the most IL-6 and TNF-α gene expression was observed in AgF group (P < 0.01). The lowest gene expression among vaccinated groups was observed in Ag group for IL-6 and Ag and Vac group for TNF-α. The highest HI titer was observed in Vac, VacN, AgF and AgT groups. The AgF group showed the highest cellular activity (P < 0.01). In conclusion, flagellin-adjuvanted groups showed a pro-inflammatory effect and acted similarly to or better than the Vac group. Hence, flagellin can be proposed as a potential adjuvant for ND vaccine.     

我国黄瓜抗病品种选育技术研究进展

黄瓜在我国蔬菜产业中占有重要地位,然而连片种植、重茬栽培等条件下容易滋生病菌,尤其是随着我国保护地蔬菜生产规模的扩大,棚室内的温湿度条件更利于病害的发生与流行,黄瓜生产上面临着严重的病虫害威胁,包括生理性病害、感染性病害及虫害等。本文从抗病品种选育的角度分析了黄瓜病害抗病品种选育技术,并简要介绍了其他防治方法,展望了相关研究的发展方向,为黄瓜病虫害的防治提供参考。     

5种生物制剂对马铃薯种薯萌芽、极端天气下植株生长和产量性状的影响

旨在满足马铃薯生产中茬口衔接、机械化生产技术应用、不利气候下稳产等对马铃薯出苗早、齐、壮的需求,以‘费乌瑞它’为供试品种,用基于有益活菌或工程菌提取物的5种生物制剂进行种薯处理,对多重性状进行了对比分析。5种生物制剂较常规化学制剂,均能够不同程度地促进种薯萌芽和芽根同生,出苗期提前2~7天,播种后49天的出苗率提高3.33%~17.78%。其中,表现最好的为酵母核苷酸衍生物和VDAL,种薯萌发和生根均显著高于对照。霜冻后,生物剂拌种处理在恢复前期促进植株生长,由此促进恢复后期的块茎发育,较常规化学处理增产8.39%~24.03%,体现了不同程度的保产效果。多马道黑、酵母核苷酸衍生物、根肽和VDAL体现出较好的保产效果,可作为种薯处理剂投入马铃薯生产。     

病程相关蛋白在采后果蔬诱导抗病性中的研究进展

果蔬受到真菌、细菌或病毒侵染时,自身能诱导产生病程相关蛋白(Pathogenesis-related proteins,PRs)。根据它们的结构亲缘关系和生物活性,PRs被分为17个功能家族。在控制果蔬采后病害的研究中,利用激发子诱导产生果蔬抗性已逐渐成为果蔬采后病害防治中一种安全、高效的保鲜方法,这也成为果蔬采后抗病研究的热点和发展趋势。本文综述了果蔬病程相关蛋白的分类、功能、诱导表达,以及蛋白组学技术在果蔬采后PRs表达水平上的应用,以期为果蔬病程相关蛋白的研究提供借鉴和参考。