中国荔枝文化遗产的特点、价值及保护——基于岭南荔枝种植系统(增城)的实证研究

中国是荔枝的原产地,是荔枝产业第一大国,拥有全球最丰富、最优质的荔枝品种。同时荔枝历史上有"百果之王"等美称,是中国文化底蕴最为深厚的果品之一。荔枝文化遗产极具中国特色且拥有全球影响力,对其保护与发展开展研究具有重要意义。本文以中国重要农业文化遗产岭南荔枝种植系统(增城)为案例,采用实地调查、深度访谈等研究方法,对荔枝文化遗产的特点、价值及保护进行了探讨。遗产地荔枝栽培历史悠久,‘挂绿’驰名中外,种质与古树资源丰富,拥有完备的生产技术体系,荔枝文化资源厚重多元。岭南荔枝种植系统(增城)是一个生态、经济与文化价值俱佳,具有南亚热带特色的生产和文化系统,但当前面临着城镇化与现代农业发展的冲击、古荔树保护力度不够、遗产价值认知不足等威胁。提出以下遗产保护与发展的建议:选择山枝与水枝的代表性区域,建设田园空间博物馆;实施古荔树保护工程,强化古树的管理与护养;加大荔枝文化普及力度,提升民众文化自觉能力;以荔枝产业园、特色小镇、果场为重点,推动荔枝产业升级发展。荔枝相关遗产地应联合申报全球重要农业文化遗产,以推动中国荔枝文化遗产的进一步保护与宣扬。     

浅析网络时代大学生自我管理能力的培养

良好的自我管理是大学生文明成人,健康成才,和谐成功的基础。自我管理能力是当代大学生必备的素质,也是我国高教体制改革的必然要求。在互联网时代,受到各种信息的冲击,加强大学生自我管理能力的培养是大学生适应社会发展、有效开发自身潜能、提高自身综合素质的根本需要。本文就网络时代下大学生做好自我管理的重要性,现实状况以及网络时代下如何培养大学生的自我管理能力进行简要分析,为网络时代下提高自我管理能力提供现实参考。     

南洋楹种源家系试验

对21个南洋楹种源/家系进行试验.结果表明:参试种源/家系4.5年生的保存率、生长性状以及形质性状存在显著或极显著差异;通过多性状综合评价,选择出具有较高生长量、较优形质性状和较强适应性的优良种源/家系7个,分别是C9、M15、P10、P6、P2、C1和C6,其中C9、M15表现最佳,4.5年生平均树高、胸径和材积分别为13.03 m、13.05 cm和0.090 79 m3,分别比对照高出13.90%、9.02%和12.80%,比对照树种马占相思高出26.63%、10.41%和74.92%.     

增城市集体林权制度改革中的科技服务创新

文章介绍了广东省增城市集体林权制度改革概况,在此基础上讨论了林业科技服务创新在社区实用技术的研究,并就科技推广服务体系建设和政策法规研究等方面如何适应广东集体林权改革进行了探讨。     

Isolation,Identification and Phylogenetic Analysis of Important Functional Genes of Porcine Pseudorabies Virus LC Strain

To find out the reason of the reproductive failure in pregnant sows in a hoggery in Guangdong, the mixture with brain, lymph nodes and lungs of farm abortion stillbirth were identified by PCR, virus isolation and culture, tissue culture infective dose (TCID₅₀) assay, homology and phylogenetic analysis of the important functional genes (gB, gC, gD and gE) and animal test. The results showed that the mixture was proved to be porcine pseudorabies virus (PRV) positive samples. The typical cytopathogenic effect was induced in the third passage of Vero cell and the titer of the fifth passage was 10^-6.8/0.1 mL. The sequence analysis and phylogenetic relationship of gB, gC, gD and gE genes showed that it was a Chinese PRV variant, which was named as LC strain. The typical pseudorabies clinical and pathological symptoms were presented in 12-week-old piglets inoculated with LC strain. The results demonstrated that a local pseudorabies virus had been isolated, suggesting that the Bartha-K61 vaccine was not fully effective for controlling the current epidemic of pseudorabies in China.     

猪伪狂犬病病毒LC株的分离鉴定及其重要功能基因的进化分析

为确诊广东某猪场母猪流产发病原因,本研究收集该猪场流产死胎的脑、淋巴结、肺脏混合液,进行PCR鉴定、病毒分离培养、半数组织培养感染剂量（tissue culture infective dose,TCID₅₀）测定、猪伪狂犬病病毒（pseudorabies virus,PRV）重要功能基因（gB、gC、gD、gE）序列测定和进化分析及动物回归试验。结果显示,病料混合液为PRV阳性,接种Vero细胞传至第3代即出现稳定的细胞病变（CPE）,第5代TCID₅₀达到10^-6.8/0.1 mL,PRV gB、gC、gD、gE基因序列测定、同源性及进化树分析显示为,PRV中国变异株,命名为LC株。动物试验显示,LC株对12周龄猪具有一定致病性,可形成PRV典型临床症状及病理变化。本研究分离到一株PRV流行毒株,推测当前使用疫苗Bartha-K61株尚无法完全控制新毒株的流行。     

Effects of TREM-2 silencing on migration and invasion abilities of rheumatoid arthritis fibroblast-like synoviocytes

AIM: To investigate the effects of triggering receptor expressed on myeloid cells-2 (TREM-2) silencing on migration and invasion abilities of rheumatoid arthritis fibroblast-like synoviocytes (RA-FLS). METHODS: Small interference RNA (siRNA) specifically targeting TREM-2 gene was transfected into RA-FLS. The interference efficiency of TREM-2 siRNA on the production of TREM-2 mRNA and protein was determined by RT-PCR and Western blot. The cell activity was assessed by CCK-8 assay. The migration and invasion abilities of RA-FLS were determined by Transwell assay. The releases of MMP-2 and MMP-9 in RA-FLS were analyzed by ELISA. The influence of TREM-2 on PI3K/AKT signal pathway was measured by Western blot. RESULTS: TREM-2 siRNA significantly decreased the mRNA and protein expression of TREM-2. No difference of cell activity between TREM-2 siRNA group and control group was observed. Transwell migration assay showed that RA-FLS through the Transwell membrane in TREM-2 siRNA group were more than the blank control group and the NC-siRNA group. In Transwell invasion assay, RA-FLS through the Transwell membrane in TREM-2 siRNA group were more than the blank control group and the NC-siRNA group. After transfected with TREM-2 siRNA, the MMP-2 secretion and phosphorylation of AKT increased significantly, while the MMP-9 secretion was not changed. CONCLUSION: TREM-2 may play an important role in the migration and invasion of RA-FLS through regulating the activation of PI3K/AKT signal pathway.     

夏季采用湿帘通风系统的密闭式肉种鸡舍温热环境因子的变化

选择华南农业大学增城正大肉鸡发展中心的肉种鸡舍为研究对象,在夏季分别对该鸡舍的气温、气湿、风速等温热环境因子进行测定,用以评价该鸡舍采用的湿帘通风系统对鸡舍内温热环境因子的调节效果。结果表明,夏季采用湿帘通风系统的鸡舍平均舍温比舍外气温低3~4℃,相对湿度保持在80%左右;夏季采用湿帘通风系统可以有效地降低舍温,主要表现为前端降温效果明显,后部降温效果有所下降;中间比两侧的降温效果好。表明夏季采用湿帘通风系统对种鸡舍内的温热环境控制较好。     

南瓜汁的酶解法制取及其在发酵面制品中的应用

研究了南瓜酶法制汁工艺,确定了最佳酶法制汁工艺参数。结果表明:面粉中添加一定量的南瓜能够改善制品的色泽,增加制品的光泽,还能改善产品的质地和风味。但添加量过高会直接影响制品的感官品质,降低面坯的发酵速度;添加过少则失去研究的意义。500 g面粉中添加125 g南瓜汁、3 g酵母调制面团,经2 h发酵,所得制品最优。     

表达猪生长激素基因的重组腺病毒的构建及对猪生长的促进效果观察

利用复制缺陷型重组腺病毒作为基因表达载体,将猪生长激素（pGH）基因直接转导到猪体内细胞,研究猪生长激素基因在猪体内的促生长作用.用同源重组的方法,构建含pGH基因的重组腺病毒（Ad-pGH）.将其感染293细胞和PK-15细胞,均可检测到pGH.用1 mL此重组腺病毒（1010TCID50）一次性肌肉注射60日龄的杂交长白猪.结果表明,试验组的猪平均增重比对照组的猪增加37%（注射后第4周）和19%（注射后第6周）;饲料报酬提高28%（注射后第4周）和18%（注射后第6周）.试验结果证明,由重组腺病毒表达的pGH具有生物学活性,在猪体内起到明显的促进生长的作用.重组腺病毒介导的pGH基因在猪体内的表达可维持约4周.