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Ji Guo-zhen Wang Li 《东北农业大学学报(英文版)》2014,21(4):31-36
Daidzein is always added into poultry feed to make the production performance and immunity of poultry better. In this study, a total of 600 40-week-old Hy-Line variety brown layers were randomized into five groups and fed with a corn-soybean-mixed basal diet supplement with 0, 10, 50, 100, and 500 mg · kg^-1 daidzein, respectively. Then, two PCR-based typing methods(RAPD-PCR and ERIC-PCR) were combined to analyze the ileum content and explore the changes of ileum microflora biodiversity. The results of RAPD-PCR and ERIC-PCR showed that bands under 10 mg · kg^-1 and 50 mg · kg^-1 were the most, and their similarity was the largest. Bands under 500 mg · kg^-1 were the least and similarity with other groups was the minimum. Ileum microflora biodiversity under 10 mg · kg^-1 or 50 mg · kg^-1 was richer than that under 500 mg · kg^-1. A corn-soybean-mixed basal diet supplement with 10 mg · kg^-1 to 50 mg · kg^-1 of daidzein might be beneficial to Hy-Line variety brown layers intestinal bacteria. 相似文献
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Chang‐Hoon Shin Hien Thi Dieu Bui Samad Rahimnejad Ji‐Hoon Cha Byung‐Woo Yoo Bo‐Kyeun Lee Hyung‐Jin Ahn Soo‐Il Choi Yun‐Jeong Choi Yong‐Ho Park Jeong‐Dae Kim Kyeong‐Jun Lee 《Journal of the World Aquaculture Society》2014,45(3):258-268
This study was conducted to evaluate the effects of dietary supplementation of Barodon, an anionic alkali mineral complex, on growth, feed utilization, humoral innate immunity and disease resistance of olive flounder. A basal experimental diet was used as a control and supplemented with 0.1, 0.2, 0.3, 0.4, or 0.5% Barodon. Triplicate groups of fish (26.4 ± 0.2 g) were fed one of the diets to apparent satiation twice daily for 10 wk. The growth performance was enhanced (P < 0.05) linearly and quadratically in fish fed diets containing Barodon compared with that in fish fed the control. Feed utilization was significantly improved by Barodon supplementation. Serum lysozyme and antiprotease activities were increased quadratically in Barodon fed groups. Also, significantly higher superoxide dismutase activity was found in Barodon‐fed fish. Dietary supplementation of 0.1–0.3% Barodon resulted in significant enhancement of fish disease resistance against Streptococcus iniae. The findings in this study indicate that dietary supplementation of Barodon can enhance growth, feed utilization, innate immunity, and disease resistance of olive flounder and that the optimum level seems to be 0.1% in diets. 相似文献
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本研究通过细胞融合、间接ELISA方法筛选出4株可稳定分泌犬瘟热病毒(canine distemper virus,CDV)和犬细小病毒(canine parvovirus,CPV)单克隆抗体的杂交瘤细胞株,以此为基础研制了一种可同时检测CDV和CPV的胶体金二联检测卡,并对其特异性、灵敏度及准确性进行了测试。结果显示:4株杂交瘤细胞可稳定传代,分泌的单克隆抗体纯度高,效价均在1:320以上。制备的胶体金二联检测卡特异性强,只与CDV和CPV产生特异性条带,而不与其他犬类病毒反应;灵敏度高,最低检测限可达到102拷贝/μL;准确性好,与荧光定量PCR结果的符合率高于88.0%,与市面上单一病毒检测卡的符合率高于97.2%。结果表明,本研究制备的CDV、CPV胶体金二联检测卡特异性强、灵敏度高、准确性好,兼具操作简便、肉眼可判读、结果易保存和无需特殊仪器设备等优势,可用于两种疾病的临床快速诊断和大规模检测工作。 相似文献
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黄土丘陵区白羊草与达乌里胡枝子混播草地土壤呼吸日变化特征 总被引:1,自引:0,他引:1
土壤呼吸是反映土壤质量和肥力性状的重要指标,阐明禾―豆混播草地土壤呼吸作用的变化规律及其影响因素,可以为准确评估混播草地的环境效应及响应提供依据。在2011和2012年6月份,采用动态密闭气室分析法,比较了白羊草(Bothriochloa ischaemum)与达乌里胡枝子(Lespedeza davurica)不同比例间作混播草地总土壤呼吸速率的日变化特征、混播植物贡献率以及温度敏感性等。结果表明,不同混播比例草地的总土壤呼吸速率日变化为单峰型曲线,峰值出现在12:00~14:00。总土壤呼吸速率日均值高低顺序为单播白羊草白羊草和达乌里胡枝子间作单播达乌里胡枝子。不同间作比例草地的总土壤呼吸速率与白昼气温相关显著(P0.05),而与5、10和15 cm土层白昼土壤温度关系不明显;单播条件下,白羊草草丛的土壤呼吸对温度的敏感性高于达乌里胡枝子;各草地土壤呼吸Q10值的变化范围为1.48~2.61,以单播白羊草最高,单播达乌里胡枝子和两者间作混播草地间无显著差异(P0.05)。 相似文献
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Beef quality traits of heifer in comparison with steer,bull and cow at various feeding environments 下载免费PDF全文
Bandugula Venkata Reddy Allur S. Sivakumar Dawoon W. Jeong Yang‐Byung Woo Sang‐June Park So‐Young Lee Ji‐Yeon Byun Chang‐Ho Kim Soo‐Hyun Cho Inho Hwang 《Animal Science Journal》2015,86(1):1-16
The present review has been focused largely on the sex type differences in beef quality among heifers, cows, steers and bulls in various feeding environments. Genetic groups, feeding systems and gender are the major factors that change carcass characteristics and fatty acid profiles of cattle. Studies identified that heifer beef has super characteristics in eating quality and a better healthy composition in fatty acids than steer, cow and bull. Diet influences the variation of fatty acid profile; particularly the level of polyunsaturated fatty acids (PUFA) interacts with breed and sex. Animals finished in pasture systems were reported to show better ratios of PUFA/ saturated fatty acids and n‐6/n‐3. Carcasses of roughage‐fed beef are lighter and have less marbling and lower quality grades but have higher cutability than carcasses of grain‐fed bulls. Heifers and cows are reported to deposit more fat than steers and bulls. Among males, lower production of testosterone by steers favors more fat thickness compared with bulls. Marbling greatly varies among cattle belonging to different sexes, and particularly, females have genetic makeup that efficiently controls deposition. The current review identified that heifers can be a premium beef brand, while steer beef currently take a large part of market share across the world. 相似文献
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芳樟工厂化育苗技术研究 总被引:10,自引:1,他引:10
通过对芳樟工业原料林良种茎尖的离体培养和繁殖 ,探讨芳樟茎尖诱导、分化、不定芽生长分化、生根的适宜的培养基和适宜的培养条件。结果表明 :适宜芳樟茎尖诱导培养基为MS +6 -BAa~ 2amg .L-1+NAAbmg .L-1,诱导率达 70 %以上 ,适宜分化丛生芽的理想培养基为改良MS +6 -BAa′~ 2a′mg .L-1+NAA0~ 2bmg .L-1+GA0~cmg .L-1芽年增殖系数达4 5 12 以上 ,有效芽苗率达 80 %以上 ,适合生根培养基为 1/2改良MS +NAAb′mg .L-1+IBA0~b′mg .L-1或 1/2改良MS +IBAb′mg .L-1+NAA0~b′mg .L-1,或R +IBAc2 mg .L-1+NAAc1 mg .L-1生根率达 98%以上。移栽土壤基质以黄心土加沙(10∶1)理想 ,移栽成活率可达 85 %以上 相似文献
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