桑悬浮细胞原生质体培养的研究

采用继代培养三个月后的桑子叶悬浮细胞为材料,进行了原生质体的分离和培养。桑悬浮细胞在纤维素酶、果胶酶、半纤维素酶的混合溶液中,酶解获得产量高、活力强的原生质体。原生质体在K8p(附加6—BA、NAA、2,4—D、LH)液体培养基中,再生细胞经多次分裂,得到肉眼可见的小愈伤组织。再通过增殖继代培养,获得浅黄色、具有明显颗粒结构的愈伤组织,转至各种激素含量的MSB固体培养基中,尚未获得绿苗分化。     

BA和激素对试管番茄愈伤组织形态发生的影响

用番茄‘Bonny Best’品种的子叶和下胚轴作为外植体,在MS附加不同水平的BA和激素的培养基中,愈伤组织形态发生的情况是不同的。本试验结果表明:在相同的植物生长调节剂水平下,番茄子叶外植体较下胚轴外植体更容易形成愈伤组织和生根;番茄子叶和下胚轴愈伤组织的形成要求不同的激素水平,较高的水平适合于子叶愈伤组织的生长,而下胚轴愈伤组织的生长要求较低的激素水平;较高浓度的BA诱导子叶和下胚轴外植体形成白色紧密的愈伤组织,而且茎的分化出现较早,较低水平的BA产生疏松的愈伤组织,分化效迟;在MS BA22.2-44.4μM IAA1-5μM的培养基上,番茄子叶和下胚轴切段可直接分化成茎;在无激素MS培养基上,下胚轴切段可直接成苗;疏松的愈伤组织是进行悬浮培养的良好材料,可利用它们进行原生质体培养和抗性克隆的筛选。     

Effects of Boron and Calcium Supply on Calcium Fractionation in Plants and Suspension Cells of Rape Cultivars with Different Boron Efficiency

Abstract

In this study, the effects of boron (B) and calcium (Ca) supply on Ca fractionation in suspension cells and different tissues of rape (Brassica napus L.) plants of two cultivars with different B efficiency were studied, with a purpose to elucidate the mechanism by which B affects Ca concentration in plants. As Ca supply increased in nutrient solution or culture medium, the relatively easily extractable Ca fractions, that is H₂O and 80% ethanol extractable Ca in leaves, 1 mol L^?1 NaCl extractable Ca in upper leaves, roots and suspension‐cell were significantly increased. While the recalcitrant Ca fractions extracted by 2% acetic acid, 0.6 mol L^?1 HCl and Ca in the residue were not affected by Ca supply. Increasing B supply in nutrient solution or culture media significantly reduced 1 mol L^?1NaCl extracted Ca in suspension cell and roots of both cultivars, which were most likely related to the alteration of cell wall metabolism. Calcium extracted by 2% acetic acid, 0.6 mol L^?1 HCl and Ca in residue in suspension‐cell and roots of B inefficient cultivar Bakow were easily improved by B deficiency as compared to that of B efficient cultivar Tezao16. Increasing of these relative recalcitrant Ca fractions was related to the different response of cultivars to the B deficiency, which may reflected different extent that Ca deposited in the two cultivars due to impaired membrane integrity under B deficiency. The effects of B on Ca concentration in lower and upper leaves of the two cultivars were quite different and were the integrated effects of B on Ca metabolism, Ca transport in plants and growth of certain organ. Increasing B supply increased total Ca concentration in upper leaves of Bakow and reduced that of Tezao16, which might relate to the different adaptability of the two cultivars to comparatively higher B supply.     

氟苯尼考双混悬型乳剂的体外药效学研究(英文)

[目的]为了探明氟苯尼考双混悬型乳剂的体外药效学特征。[方法]以氟苯尼考注射液为对照组对氟苯尼考双混悬型乳剂进行5种细菌的最低抑菌浓度(MIC)、最低杀菌浓度(MBC)和耐药突变选择窗(MSW)的测定,对鼠伤寒沙门氏菌进行抗生素后效应(PAE)和抗生素后亚抑菌浓度效应(PASME)的测定。[结果]相对于普通注射液氟苯尼考双混悬型乳剂对5种细菌的MIC和MBC无明显变化,但其明显缩小了对5种细菌的MSW(P<0.01),并且明显的延长了对鼠伤寒沙门氏菌PAE和PASME(P<0.01)。[结论]氟苯尼考双混悬型乳剂在体外能够减小细菌耐药几率,明显延长抗生素对细菌的后效应时间,从而有效地提高抗菌效果。     

Apoplastic redox metabolism: Synergistic phenolic oxidation and a novel oxidative burst

The plant apoplast is an important mediator of communication between the cell cytoplasm and its surroundings. Plant cell suspensions offer a convenient model system to gain insight into apoplastic physiology. Here, we describe a novel phenomenon that took place when two naturally occurring phenolics were added together to either soybean or tobacco cell suspensions. Acetosyringone (AS) and/or hydroxyacetophenone (HAP), phenolics found in the extracellular/apoplast of tobacco cells, were added to soybean or tobacco cell suspensions undergoing an oxidative burst. Individually, AS appeared to be utilized as a typical peroxidase substrate to scavenge hydrogen peroxide, while HAP was utilized at a much lower rate. However, when added together the rate of utilization of both phenolics increased and surprisingly resulted in the production of hydrogen peroxide. We have further characterized this novel phenomenon in suspension cells. This study demonstrates that certain phenolics in plants can cause co-oxidation which, as in animals, could alter the structure and bioactivity of surrounding phenolics.     

四川‘竹根姜’胚性细胞悬浮系的建立与植株再生

 选用四川省地方品种‘竹根姜’的茎尖组织建立了体细胞胚性细胞悬浮系, 并通过悬浮培养获得了姜再生植株。结果表明悬浮细胞干质量受pH影响。接种量和AgNO₃ 对悬浮细胞的生长都有影响:最佳接种量为1.0% , 最适AgNO₃浓度为6.0 mg·L ^{- 1}。胚性细胞悬浮系增殖后, 接种到前期筛选的成熟分化培养基上获得了再生植株。     

药用植物半夏细胞悬浮培养和植株再生体系建立研究

为了建立甘肃产药用植物半夏的细胞悬浮培养和植株再生体系,通过对其不同外质体筛选、愈伤组织诱导和悬浮培养的愈伤组织类型、激素水平、起始密度、震荡速率等影响因素以及细胞生长状态、生长曲线和pH值变化等指标的研究.结果表明:以叶柄为外质体,选择"松散型"愈伤组织和2×105个/mL的起始培养密度,在附加2,4-D 2.0 mg/L BA 0.5 mg/L NAA0.5 mg/L的1/2 MS培养基上培养,温度(23±2)℃,转速90 rpm,pH值6.0,黑暗培养为最适悬浮培养体系;经检测细胞生长曲线呈"S"型,培养液pH随着细胞的生长先上升后下降,18 d时细胞基数达到最大值;悬浮继代培养后,通过降低或停止震荡转速,在添加BA 0.5 mg/L NAA0.5 mg/L的MS液体和固体培养基上可诱导分化出芽和根,进一步形成完整的植株.     

430g/L戊唑醇水悬浮剂的研制

戊唑醇是一种广谱内吸型杀菌剂,对多种作物的病害有较好防治效果。本文介绍了农药悬浮剂发展、加工及实验理论依据,并采用优化组合法对表面活性剂进行了筛选。试验结果表明:该产品悬浮率90%以上,热贮(54℃±2℃,14d)分解率<5%,产品各项指标符合悬浮剂的要求。     

尤溪口闽江多跨悬索桥

本文简述尤溪口闽江多跨悬索桥的主要结构、施工安装及其工作原理。     

胡萝卜悬浮细胞系及高效再生系统的建立

以胡萝卜块根为材料诱导愈伤组织,并建立悬浮细胞系,然后将悬浮细胞和愈伤组织分别转入分化培养基进行分化培养。结果表明,胡萝卜块根形成层愈伤组织诱导率为100%,最佳激素种类和用量为2,4!D0.1mg/L;根据外观可将愈伤组织大致分为4种类型,I型是直接建立悬浮细胞系的良好来源,Ⅲ型经继代培养后可用于悬浮细胞系的建立;静止法是胡萝卜悬浮细胞系简单而有效的继代方法;胡萝卜愈伤组织分化的最佳培养基是MS或B5基本培养基,MS培养基上的再生苗可直接生根,而B5培养基上的再生苗在原培养基上很难生根,需要转移到MS培养基上才能生根。