基于优势分析法的西印度洋捕捞国和地区渔获量差异初步分析

西印度洋是海洋捕捞作业的重要海域,分析捕捞方渔获种类组成和渔获量是评价渔业资源利用状态的基础,也是确保其资源可持续开发和科学管理的重要支撑。本研究根据FAO提供的1950-2019年西印度洋海域渔获量数据,采用优势分析(Dominance analysis),分析了70年间主要捕捞方渔获种类和渔获量组成,比较不同时间序列下主要捕捞方和种类对渔获量的贡献率。研究表明,西印度洋海域渔获量整体呈上升趋势,根据突变点检测及渔获量增长趋势可划分为1950-1982年、1983-2005年和2006-2019年三个阶段。研究分析表明,各阶段累计渔获量前十的渔获种类、捕捞方不存在显著性差异(P<0.05)长头小沙丁鱼(Sardinella longiceps)累计渔获量为最高,鲣、黄鳍金枪鱼等种类渔获量对总渔获量的贡献度较高;印度、巴基斯坦和伊朗等是沿海国和地区各阶段渔获量最高的捕捞方,西班牙、法国、日本和中国台湾省等是非沿海国和地区渔获量最高的捕捞方,对各阶段印度洋西部海域总渔获量的贡献度基本都超过10%。。研究认为,基于印度洋西部海域渔业资源开发现状及其潜力,各主要捕捞国家和地区应该降低捕捞努力量,养护已过度开发的经济种类,增加对资源量丰富的短生命周期种类的开发力度,确保印度洋西部海域渔业资源可持续利用。     

Production of polyclonal antiserum against recombinant VP28 protein and its application for the detection of white spot syndrome virus in crustaceans

The VP28 gene of white spot syndrome virus (WSSV) was cloned into pRSET B expression vector. The VP28 protein was expressed as a protein with a 6-histidine taq in Escherichia coli GJ1158 with NaCl induction. Antiserum was raised against this recombinant-VP28 protein in rabbits and it recognized VP28 protein in naturally and experimentally WSSV-infected shrimp, marine crabs, freshwater prawns and freshwater crabs. The antiserum did not recognize any of the other known WSSV structural proteins. Various organs such as eyestalks, head muscle, gill tissue, heart tissue, haemolymph, tail tissue and appendages were found to be good materials for detection of WSSV using the antiserum and detection of WSSV was successful in experimentally infected Penaeus monodon and P. indicus at 12 and 24 h post-infection (p.i.), respectively. The antiserum was capable of detecting WSSV in 5 ng of total haemolymph protein from WSSV-infected shrimp.     

近代广东住宅园林布局特点浅析

因受特定的历史背景、人文地理和气候环境的影响,近代广东住宅园林独具特色,既继承了传统岭南园林的环境适应性和人文要素,又扬弃融合了西方园林的造园手法。文章着重研究近代广东住宅园林的布局特点,特别是中西合璧的造园手法所形成的布局样式和风格,为现代园林的设计与实践提供新的视角。     

Expression of recombinant triangular bream (Megalobrama terminalis) pre-IGF-I in Escherichia coli

The insulin-like growth factor I (IGF-I) cDNA (GenBank Accession No. AY247412) of triangular bream (Megalobrama terminalis) was expressed for the first time in Escherichia coli. To construct the expression plasmid, the IGF-I cDNA was subcloned into prokaryotic-expressing vector pGEX-4T-1. The E.coli JM109 was transformed with the recombinant plasmid pGEX-4T-1-IGF-I, and the transgene expression was observed after being induced with isopropyl-β-D-thiogalactoside (IPTG). The results of SDS polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting indicated that the recombinant fusion protein had immune activity and the molecular weight was about 47 kDa. The results of SDS-PAGE and thin layer scanning showed that the yield of fusion protein had been enlarged with prolonging time. When the time of induced expression was 1, 2, 3, 4, 5, and 6 h, the expression amount was approximately 1.4, 4.3, 8.1, 11.3, 16.3 and 18.8% of total bacterial protein respectively.     

Excavating sponges that are destructive to farmed pearl oysters in Western and Northern Australia

Species of the family Clionaidae (Porifera: Demospongiae) that excavate shell of the silver‐lip pearl oyster, Pinctada maxima, in north Western and Northern Australia are described. Two species belong to the genus Cliona and one species to the closely related genus Pione. Cliona orientalis has only recently been reported from Australia in living and dead coral on the Great Barrier Reef, and this is the first report of this species from north Western and Northern Australia. Cliona dissimilis is reported from Australia for the first time. Pione velans was first described from Shark Bay, Western Australia and this study extends its distribution from Albany, south Western Australia to Port Bremer in the Northern Territory. The most common species found was C. dissimilis. Pione velans and C. orientalis were also present, although the latter species was rare. Cliona dissimilis and P. velans were found to be sexually reproductive in some shells with specimens of C. dissimilis with oocytes in May and P. velans in September 1999. Egg development was synchronous, indicating that the sponges were oviparous and would broadcast gametes in a spawning event.     

副溶血弧菌的外膜蛋白及其抗原性研究↑（*）

本文对１３株不同来源的副溶血弧菌的外膜蛋白及其抗原性进行了比较研究。１３株副溶血弧菌的外膜蛋白有相似的ＳＤＳ－ＰＡＧＥ图谱。大多数菌株有五条主要的蛋白质，其大致分子量分别为：ａ、６９ｋＤａ；ｂ、６３ｋＤａ；ｃ、４０ｋＤａ；ｄ、３０ｋＤａ；ｅ、２８ｋＤａ。其中蛋白质ｂ是所有菌株共有的。与吸附后的兔抗副溶血弧菌血清Ｗｅｓｔｅｒｎ印迹法结果显示抗血清与多数副溶血弧菌菌株的外膜蛋白有一条共同的免疫反应带，其大致分子量为４４ｋＤａ。     

橡胶树肌动蛋白解聚因子原核表达及纯化

本研究根据HbADF序列设计引物扩增基因的编码区,并将其插入到原核表达载体pET28a上,成功构建重组质粒pET28a-HbADF。将重组质粒转化大肠杆菌BL21(DE3)宿主菌,经1 mmol/L IPTG诱导,获得相对分子量为20 ku的融合蛋白。表达蛋白以可溶和包涵体两种形式存在,通过亲和层析方法纯化可溶蛋白并获得HbADF融合蛋白,用抗HIS标签的单抗对纯化蛋白进行了Western blot鉴定。该结果为进一步研究HbADF蛋白特性及功能奠定基础。     

粤西地区甘蔗、香蕉产业发展现状调研分析

简要分析粤西地区甘蔗和香蕉产业发展现状和存在问题,针对存在问题及其今后的发展重点,提出粤西地区甘蔗、香蕉产业发展重点及对策,以促进粤西地区甘蔗和香蕉产业稳健发展。     

中华蜜蜂囊状幼虫病病毒非结构蛋白抗体的制备及其应用

应用Gateway重组技术构建CSBV非结构蛋白,RNA依赖的RNA聚合酶(RNA-dependent RNA polymerase,RdRp)的原核表达载体,将重组表达载体转化大肠杆菌Rosetta,经异丙基硫代-β-D-半乳糖苷(Isopropylβ-D-1-Thiogalactopyranoside,IPTG)诱导获得RdRp基因原核表达蛋白.以重组蛋白为抗原免疫新西兰兔制备相应的多克隆抗体,Western blot检测表明,该抗体能与RdRp重组蛋白和感病中华蜜蜂幼虫蛋白特异性结合,说明获得的抗体特异性高.利用制备的抗体进行免疫荧光标记,发现其能特异地定位在感病中华蜜蜂血淋巴细胞内.     

2011年鄂西地区水稻稻瘟病菌遗传多样性的分析

[目的]扩大鄂西地区稻瘟病菌菌库,分析其群体结构.[方法]收集鄂西不同地区2011年间的感病稻秆,分离保存稻秆上的穗颈瘟病菌,通过RAPD、rep-Pot2-PCR、REMAP 3种标记对分离的菌株进行分子标记.[结果]从9个稻秆上共分离得到29个稻瘟病菌.遗传多样性分析结果表明,相似性水平在75％时,2011年供试菌株可分为5个遗传宗谱,宗谱3（7个菌株）和4（19个菌株）为优势宗谱,其余3个为稀有宗谱.[结论] 2011年鄂西地区稻瘟病菌由5个遗传宗谱组成.