| 2011年鄂西地区水稻稻瘟病菌遗传多样性的分析 |
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| 引用本文： | 李,泌,王春台,周,杰等.2011年鄂西地区水稻稻瘟病菌遗传多样性的分析[J].安徽农业科学,2014(14):4249-4251. |
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| 作者姓名： | 李 泌 王春台 周 杰等 |
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| 作者单位： | 中南民族大学生命科学学院／生物技术国家民委重点实验室,武陵山区特色资源植物种质保护与利用湖北省重点实验室,湖北武汉430074 |
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| 基金项目： | 湖北省自然科学基金创新群体“武陵山区稻瘟病菌收集分类与抗病育种研究”（2011CDA125）;湖北省自然科学基金面上项目“稻瘟病抗性新品种的培育”（2013CFB453）. |
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| 摘 要： | 目的]扩大鄂西地区稻瘟病菌菌库,分析其群体结构.方法]收集鄂西不同地区2011年间的感病稻秆,分离保存稻秆上的穗颈瘟病菌,通过RAPD、rep-Pot2-PCR、REMAP 3种标记对分离的菌株进行分子标记.结果]从9个稻秆上共分离得到29个稻瘟病菌.遗传多样性分析结果表明,相似性水平在75％时,2011年供试菌株可分为5个遗传宗谱,宗谱3（7个菌株）和4（19个菌株）为优势宗谱,其余3个为稀有宗谱.结论] 2011年鄂西地区稻瘟病菌由5个遗传宗谱组成.
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| 关 键 词： | 水稻稻瘟病菌 鄂西地区 遗传多样性 |
Genetic Diversity of Magnaporthe oryzae in Western Hubei Province in 2011 |
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| LI Mi,XU Xin.Genetic Diversity of Magnaporthe oryzae in Western Hubei Province in 2011[J].Journal of Anhui Agricultural Sciences,2014(14):4249-4251. |
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| Authors: | LI Mi XU Xin |
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| Institution: | et al ( Hubei Provincial Key Laboratory for Protection and Application of Special Plants in Wuling Area of China, Key Labora- tory of State Ethnic Affairs Commission for Biological Technology, College of Life Science, South - Central University for Nationalities, Wuhan, Hubei 430074) |
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| Abstract: | Objective] To enlarge the physiological race library of Magnaporthe oryzae in Western Hubei Province,analyze the population structure.Method] The Magnaporthe oryzae spores were isolated from the infected rice panicles,which were collected from western Hubei Province area in 2011.Cluster analysis of the test samples was conducted by RAPD,REMAP and rep-Pot2-PCR.Result] 29 Magnaporthe grisea strains were isolated from 9 panicles.The result of cluster analysis showed there were 5 genetic lineages at similarity coefficient of 0.75.Lineages Ⅲ and Ⅳ,which included 7 strains and 19 strains,respectively,were dominant lineage.Conclusion] Magnaporthe oryzae in West Hubei Province in 2011 was divided into 5 genetic lineages. |
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| Keywords: | Magnaporthe oryzae Western Hubei Province Genetic diversity |
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